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The map label for this gene is glnB [H]

Identifier: 28198922

GI number: 28198922

Start: 1223937

End: 1224275

Strand: Reverse

Name: glnB [H]

Synonym: PD1025

Alternate gene names: 28198922

Gene position: 1224275-1223937 (Counterclockwise)

Preceding gene: 28198923

Following gene: 28198921

Centisome position: 48.59

GC content: 44.25

Gene sequence:

>339_bases
ATGAAACTGATTACTGCCATCATTCGACCGTTCAAGCTTGATGAGGTGCGAGAAGCACTATCAAAAGTTGGCGTGTCAGG
TATTACCGTGACTGAGGTCAAGGGCTTTGGACAGCAAAAGGGTCATACTGAATTATATCGAGGGACTGAGTACGTCATTG
ATTTTTTGCCGAAAATCAAGATCGAAATGGTGGTTACCGATGAGCACTTGGATGCGGTCATTGAAGTGATCCGGATCGCC
GCTGTTACCGGAAAAATCGGTGACGGTAAAATTTTTGTCACTAAAATCGAACAGGTTGTAAGAATCCGTACTGGTGAGAT
CGACATTGATGCACTCTAA

Upstream 100 bases:

>100_bases
CGTAAGTTAGAATCCGCTTTCACTTAGAGGATGCTGCTTTGGCTTCTGATTAATCCGATATTACTGTCTGCTAGATGCAT
TCCACGATTGGGGTATGCGA

Downstream 100 bases:

>100_bases
CTCTTGCCATTTTAGTGATATGAAGAATGGTTTTTTCGCAATCTGTATATTTATCGCGTTGGCTTTGTTGGCAGGTGGCA
ATGCCATCGGTCGTGCGGAG

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLITAIIRPFKLDEVREALSKVGVSGITVTEVKGFGQQKGHTELYRGTEYVIDFLPKIKIEMVVTDEHLDAVIEVIRIA
AVTGKIGDGKIFVTKIEQVVRIRTGEIDIDAL

Sequences:

>Translated_112_residues
MKLITAIIRPFKLDEVREALSKVGVSGITVTEVKGFGQQKGHTELYRGTEYVIDFLPKIKIEMVVTDEHLDAVIEVIRIA
AVTGKIGDGKIFVTKIEQVVRIRTGEIDIDAL
>Mature_112_residues
MKLITAIIRPFKLDEVREALSKVGVSGITVTEVKGFGQQKGHTELYRGTEYVIDFLPKIKIEMVVTDEHLDAVIEVIRIA
AVTGKIGDGKIFVTKIEQVVRIRTGEIDIDAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=64.2857142857143, Blast_Score=157, Evalue=2e-40,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=66.0714285714286, Blast_Score=149, Evalue=4e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12436; Mature: 12436

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLITAIIRPFKLDEVREALSKVGVSGITVTEVKGFGQQKGHTELYRGTEYVIDFLPKIK
CCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCHHHHHHHCCCEE
IEMVVTDEHLDAVIEVIRIAAVTGKIGDGKIFVTKIEQVVRIRTGEIDIDAL
EEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MKLITAIIRPFKLDEVREALSKVGVSGITVTEVKGFGQQKGHTELYRGTEYVIDFLPKIK
CCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCHHHHHHHCCCEE
IEMVVTDEHLDAVIEVIRIAAVTGKIGDGKIFVTKIEQVVRIRTGEIDIDAL
EEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8931324; 11481430 [H]