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The map label for this gene is glnA [H]

Identifier: 28198923

GI number: 28198923

Start: 1224529

End: 1225938

Strand: Reverse

Name: glnA [H]

Synonym: PD1026

Alternate gene names: 28198923

Gene position: 1225938-1224529 (Counterclockwise)

Preceding gene: 28198925

Following gene: 28198922

Centisome position: 48.65

GC content: 49.36

Gene sequence:

>1410_bases
ATGTCGCTGGAAAATGTTGAAAAGCTTGTCAAGGATAAAAAGATCGAATTCGTCGATTTGCGCTTTGTTGATATGCGCGG
CGTACAACAGCACGTCACCTTTCCGGTGAACATTCTTGAACAATCGCTGTTCAAGGATGGAAAGATGTTCGACGGTAGCT
CGATTGCTGGTTGGAAGGGGATCAATGAGTCGGACATGGTACTGCTGCCTGACGCTAGCACCGCTTATCTGGATCCTTTT
TACGCTGATCCAACGCTGGTCATGACATGCGACATCCTTGATCCAGCGACGATGCAGAGTTATGTGCGTGATCCCCGAGG
TATCGCCAAACGTGCCGAAATCTACTTAAATTCTTTAGGTATTGCTGACCAAGCATTTTTCGGTCCGGAACCTGAATTTT
TTATCTTCGATTCAGTCCGTTTTGCTAATGATATGGGTCATGCTTTTTTCCAAATTGCTTCCGAAGAGGCTGCCTGGAAC
ACAGGCGCTGAGTTTGAGGGAGGTAACAGTGGTTACCGCCCGACGGTAAAGGGTGGATACTTCCCAGTTCCACCGACTGA
CTCGCTGCACGATTTGCGTGCCGAGATGGTGAAGACCCTGGAGCAGGTTGGCATTGAGACCGAGGTGCATCATCACGAAG
TCGCCACTGCTGGACAATGTGAGATCGGCACTAAGTTCGACTCATTGGTCAAAAAGGCTGATCAGCTGATGACGATGAAA
TACATCCTTAAAAACGTTGCTTACCGCAATGGGAAGACTGTCACCTTTATGCCTAAACCGATCGTTGGTGATAACGGTTC
TGGCATGCATGTACATCAGTCGCTCTCTAAAGGAGGGGTCAACTTGTTCTCCGGTGACGACCATGCTGGTTTGTCGCAGC
TGGCACTGTATTACATCGGCGGTATTTTCAAGCACGCTCATGCGATCAACGCGTTTTCCAATTCGGGAACAAATAGCTAT
AAGCGTTTGGTTCCGGGCTTCGAAGCACCCGTAATGTTGGCTTATTCGGCACGTAACCGCTCGGCCTCGTGCCGTATTCC
ATGGGTTTCTAGCCCGAAGGCACGCCGCATTGAAATGCGTTTCCCCGATCCATTGCAGTCTGGTTACCTCACCTTTACTG
CGCTGATGATGGCTGGACTGGATGGCATTAAAAACAAGATCCATCCAGGCCCACCCAATGACAAAGATCTGTATCACCTG
CCGCCTGAAGAGGAAAAATTGATCCCGCAGGTTTGTTCAAGTTTGGATCAGGCTCTTGAAGCATTGGACAAAGACCGCGA
TTTCTTGAAGGCAGGCAGTGTGATGAGTGACGATTTTATTAACGGCTATATTGCGCTGAAGATGCAGGAAGTGACGCAGT
TCCGCGCGGCCACACATCCGCTGGAATACCAGATGTACTACGCCAACTAA

Upstream 100 bases:

>100_bases
TACCCGAAAAAAATCAATGGATTGCAAGGTTCACAGACTTGGTATGCTAATTGCTTCAGCTGCTTCTGACTGTTACCCCA
ATCCTAAAAGGTTTCCTCTG

Downstream 100 bases:

>100_bases
GTATGTAGTGACAGCGATGGTGGAATTTTTACCTTCCACCATCACTATTTCTTCCATTTTCTTAGGGGTAGGGGAGGATC
GGGAGTCAAGCGACCTTCTG

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSLENVEKLVKDKKIEFVDLRFVDMRGVQQHVTFPVNILEQSLFKDGKMFDGSSIAGWKGINESDMVLLPDASTAYLDPF
YADPTLVMTCDILDPATMQSYVRDPRGIAKRAEIYLNSLGIADQAFFGPEPEFFIFDSVRFANDMGHAFFQIASEEAAWN
TGAEFEGGNSGYRPTVKGGYFPVPPTDSLHDLRAEMVKTLEQVGIETEVHHHEVATAGQCEIGTKFDSLVKKADQLMTMK
YILKNVAYRNGKTVTFMPKPIVGDNGSGMHVHQSLSKGGVNLFSGDDHAGLSQLALYYIGGIFKHAHAINAFSNSGTNSY
KRLVPGFEAPVMLAYSARNRSASCRIPWVSSPKARRIEMRFPDPLQSGYLTFTALMMAGLDGIKNKIHPGPPNDKDLYHL
PPEEEKLIPQVCSSLDQALEALDKDRDFLKAGSVMSDDFINGYIALKMQEVTQFRAATHPLEYQMYYAN

Sequences:

>Translated_469_residues
MSLENVEKLVKDKKIEFVDLRFVDMRGVQQHVTFPVNILEQSLFKDGKMFDGSSIAGWKGINESDMVLLPDASTAYLDPF
YADPTLVMTCDILDPATMQSYVRDPRGIAKRAEIYLNSLGIADQAFFGPEPEFFIFDSVRFANDMGHAFFQIASEEAAWN
TGAEFEGGNSGYRPTVKGGYFPVPPTDSLHDLRAEMVKTLEQVGIETEVHHHEVATAGQCEIGTKFDSLVKKADQLMTMK
YILKNVAYRNGKTVTFMPKPIVGDNGSGMHVHQSLSKGGVNLFSGDDHAGLSQLALYYIGGIFKHAHAINAFSNSGTNSY
KRLVPGFEAPVMLAYSARNRSASCRIPWVSSPKARRIEMRFPDPLQSGYLTFTALMMAGLDGIKNKIHPGPPNDKDLYHL
PPEEEKLIPQVCSSLDQALEALDKDRDFLKAGSVMSDDFINGYIALKMQEVTQFRAATHPLEYQMYYAN
>Mature_468_residues
SLENVEKLVKDKKIEFVDLRFVDMRGVQQHVTFPVNILEQSLFKDGKMFDGSSIAGWKGINESDMVLLPDASTAYLDPFY
ADPTLVMTCDILDPATMQSYVRDPRGIAKRAEIYLNSLGIADQAFFGPEPEFFIFDSVRFANDMGHAFFQIASEEAAWNT
GAEFEGGNSGYRPTVKGGYFPVPPTDSLHDLRAEMVKTLEQVGIETEVHHHEVATAGQCEIGTKFDSLVKKADQLMTMKY
ILKNVAYRNGKTVTFMPKPIVGDNGSGMHVHQSLSKGGVNLFSGDDHAGLSQLALYYIGGIFKHAHAINAFSNSGTNSYK
RLVPGFEAPVMLAYSARNRSASCRIPWVSSPKARRIEMRFPDPLQSGYLTFTALMMAGLDGIKNKIHPGPPNDKDLYHLP
PEEEKLIPQVCSSLDQALEALDKDRDFLKAGSVMSDDFINGYIALKMQEVTQFRAATHPLEYQMYYAN

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=63.2478632478632, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52160; Mature: 52029

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLENVEKLVKDKKIEFVDLRFVDMRGVQQHVTFPVNILEQSLFKDGKMFDGSSIAGWKG
CCHHHHHHHHHCCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
INESDMVLLPDASTAYLDPFYADPTLVMTCDILDPATMQSYVRDPRGIAKRAEIYLNSLG
CCCCCEEEECCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
IADQAFFGPEPEFFIFDSVRFANDMGHAFFQIASEEAAWNTGAEFEGGNSGYRPTVKGGY
CCHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
FPVPPTDSLHDLRAEMVKTLEQVGIETEVHHHEVATAGQCEIGTKFDSLVKKADQLMTMK
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
YILKNVAYRNGKTVTFMPKPIVGDNGSGMHVHQSLSKGGVNLFSGDDHAGLSQLALYYIG
HHHHHHHHCCCCEEEEECCCEECCCCCCCHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
GIFKHAHAINAFSNSGTNSYKRLVPGFEAPVMLAYSARNRSASCRIPWVSSPKARRIEMR
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEC
FPDPLQSGYLTFTALMMAGLDGIKNKIHPGPPNDKDLYHLPPEEEKLIPQVCSSLDQALE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
ALDKDRDFLKAGSVMSDDFINGYIALKMQEVTQFRAATHPLEYQMYYAN
HHHCCHHHHHHCCCCCHHHCCCEEEEEEHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SLENVEKLVKDKKIEFVDLRFVDMRGVQQHVTFPVNILEQSLFKDGKMFDGSSIAGWKG
CHHHHHHHHHCCCCEEEEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
INESDMVLLPDASTAYLDPFYADPTLVMTCDILDPATMQSYVRDPRGIAKRAEIYLNSLG
CCCCCEEEECCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
IADQAFFGPEPEFFIFDSVRFANDMGHAFFQIASEEAAWNTGAEFEGGNSGYRPTVKGGY
CCHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCE
FPVPPTDSLHDLRAEMVKTLEQVGIETEVHHHEVATAGQCEIGTKFDSLVKKADQLMTMK
ECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
YILKNVAYRNGKTVTFMPKPIVGDNGSGMHVHQSLSKGGVNLFSGDDHAGLSQLALYYIG
HHHHHHHHCCCCEEEEECCCEECCCCCCCHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
GIFKHAHAINAFSNSGTNSYKRLVPGFEAPVMLAYSARNRSASCRIPWVSSPKARRIEMR
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEC
FPDPLQSGYLTFTALMMAGLDGIKNKIHPGPPNDKDLYHLPPEEEKLIPQVCSSLDQALE
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
ALDKDRDFLKAGSVMSDDFINGYIALKMQEVTQFRAATHPLEYQMYYAN
HHHCCHHHHHHCCCCCHHHCCCEEEEEEHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]