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The map label for this gene is amtB [H]

Identifier: 28198921

GI number: 28198921

Start: 1222493

End: 1223947

Strand: Reverse

Name: amtB [H]

Synonym: PD1024

Alternate gene names: 28198921

Gene position: 1223947-1222493 (Counterclockwise)

Preceding gene: 28198922

Following gene: 28198904

Centisome position: 48.57

GC content: 48.87

Gene sequence:

>1455_bases
ATGCACTCTAACTCTTGCCATTTTAGTGATATGAAGAATGGTTTTTTCGCAATCTGTATATTTATCGCGTTGGCTTTGTT
GGCAGGTGGCAATGCCATCGGTCGTGCGGAGGCTCAGGTACAAACCAATCCTTTGCCTAGCGAGCAAGTCACCACCGAAT
TGTTGATGTCTGAAGCAACCCCTTCCGACGCGGTTGCAATACCTAAAACGACTTCACCCCCCGTGGTAGACAAGGGGGAC
GTTGCTTGGATGCTCACCTCAACGCTATTAGTGCTACTGATGGTGTTACCAGGCTTGGCACTGTTTTACGGCGGCATGTT
GCGTTCTAAAAACGTACTATCAATGATGGTACAAATTGGTGTGGTGTTTTCTCTGATAGCCATCTTGTGGGTGCTATACG
GCTATAGCCTTGCTTTTGCTGATGGTGACATGTTTATTGGTACATTCAATAAGGCATTTCTTTATGGCGTAGACACTGCA
ACGTTGGTAGATACCTTCTCTAAAGGGTTCAAACTGCCCGAGTACGTGTACGTTGCTTTCCAATTGACTTTTGCAGGTAT
CACCGCTGCATTGATTGTCGGTGCTTTTGCAGAACGCGTGAAATTTGCTGCGGTACTGCTGTTTATTTTCATTTGGTTTT
CCTTCGGCTACCTGCCACTGGCACACATGGTGTGGTCGACGCCAGGTTTTCTGTTGAGCAAGGGGGCCTTGGATTTTGCC
GGTGGGACAGTTGTACATATCAATGCTGGTATTGCTGGATTGATCGGTGCTTACTTTGTTGGTAAGCGTATTGGTTTTGG
CCAGGTTGCCGTCAAGCCGCATAACGTCCCCATGACTTTCACTGGTGCTGCACTGCTATGGGTCGGTTGGTTCGGCTTCA
ATGCTGGTTCGGCGTTAGAAGCCAATAGCATTGCTGCCTTGGCTTTCATCAACACCATGCTTGCGACTGCTGCTGCGATT
CTCGTTTGGTCGTTGCTCGAAAAGATGACCAAGGGAAAATTCTCAGCTCTGGGTGTTGCTTCAGGCATGATTGCTGGTTT
AGTAGGCGTCACTCCAGCGGCGGGTACGGTTGGTCCATTTGGTGCAATTGCGATTGGTGCTGTTGCCGCCGTGTGCTGTA
TCTGGGGCATCACTGGACTGAAAAGATTATTGAAGGTAGACGATACCTTGGACGTATTCGGCGTGCATGGCATTGGCGGC
ATTGTTGGTGCGATTTTGACTGGGGTTTTTACCGATAAGGCGCTAGGCGGTCAGGGATATGCTGAGGGGATGACAATGAG
CAATCAAGTCATGATTCAAGCCATGAGTGTGGGGATCAGCATCGTGTGGATTGGAATCGTTTCGCTGGTAGGTTTCTTAA
TTGTCAAACTGCTGTTTGGTTTGCGTGTAAGCGAAGAAGCTGAACGTGAAGGATTGGACATTACATCGCACGGTGAGTCG
GCGTACGAGTCCTGA

Upstream 100 bases:

>100_bases
ATCCGGATCGCCGCTGTTACCGGAAAAATCGGTGACGGTAAAATTTTTGTCACTAAAATCGAACAGGTTGTAAGAATCCG
TACTGGTGAGATCGACATTG

Downstream 100 bases:

>100_bases
GTCTTGTTCAGGTCACACTAAAATATTATTGCCGTGGTGCTATTACCGTGCCACGAAGTCCATGTGATGGTCATCCCCAT
AACGTTACTTATTGAATCTA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MHSNSCHFSDMKNGFFAICIFIALALLAGGNAIGRAEAQVQTNPLPSEQVTTELLMSEATPSDAVAIPKTTSPPVVDKGD
VAWMLTSTLLVLLMVLPGLALFYGGMLRSKNVLSMMVQIGVVFSLIAILWVLYGYSLAFADGDMFIGTFNKAFLYGVDTA
TLVDTFSKGFKLPEYVYVAFQLTFAGITAALIVGAFAERVKFAAVLLFIFIWFSFGYLPLAHMVWSTPGFLLSKGALDFA
GGTVVHINAGIAGLIGAYFVGKRIGFGQVAVKPHNVPMTFTGAALLWVGWFGFNAGSALEANSIAALAFINTMLATAAAI
LVWSLLEKMTKGKFSALGVASGMIAGLVGVTPAAGTVGPFGAIAIGAVAAVCCIWGITGLKRLLKVDDTLDVFGVHGIGG
IVGAILTGVFTDKALGGQGYAEGMTMSNQVMIQAMSVGISIVWIGIVSLVGFLIVKLLFGLRVSEEAEREGLDITSHGES
AYES

Sequences:

>Translated_484_residues
MHSNSCHFSDMKNGFFAICIFIALALLAGGNAIGRAEAQVQTNPLPSEQVTTELLMSEATPSDAVAIPKTTSPPVVDKGD
VAWMLTSTLLVLLMVLPGLALFYGGMLRSKNVLSMMVQIGVVFSLIAILWVLYGYSLAFADGDMFIGTFNKAFLYGVDTA
TLVDTFSKGFKLPEYVYVAFQLTFAGITAALIVGAFAERVKFAAVLLFIFIWFSFGYLPLAHMVWSTPGFLLSKGALDFA
GGTVVHINAGIAGLIGAYFVGKRIGFGQVAVKPHNVPMTFTGAALLWVGWFGFNAGSALEANSIAALAFINTMLATAAAI
LVWSLLEKMTKGKFSALGVASGMIAGLVGVTPAAGTVGPFGAIAIGAVAAVCCIWGITGLKRLLKVDDTLDVFGVHGIGG
IVGAILTGVFTDKALGGQGYAEGMTMSNQVMIQAMSVGISIVWIGIVSLVGFLIVKLLFGLRVSEEAEREGLDITSHGES
AYES
>Mature_484_residues
MHSNSCHFSDMKNGFFAICIFIALALLAGGNAIGRAEAQVQTNPLPSEQVTTELLMSEATPSDAVAIPKTTSPPVVDKGD
VAWMLTSTLLVLLMVLPGLALFYGGMLRSKNVLSMMVQIGVVFSLIAILWVLYGYSLAFADGDMFIGTFNKAFLYGVDTA
TLVDTFSKGFKLPEYVYVAFQLTFAGITAALIVGAFAERVKFAAVLLFIFIWFSFGYLPLAHMVWSTPGFLLSKGALDFA
GGTVVHINAGIAGLIGAYFVGKRIGFGQVAVKPHNVPMTFTGAALLWVGWFGFNAGSALEANSIAALAFINTMLATAAAI
LVWSLLEKMTKGKFSALGVASGMIAGLVGVTPAAGTVGPFGAIAIGAVAAVCCIWGITGLKRLLKVDDTLDVFGVHGIGG
IVGAILTGVFTDKALGGQGYAEGMTMSNQVMIQAMSVGISIVWIGIVSLVGFLIVKLLFGLRVSEEAEREGLDITSHGES
AYES

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=413, Percent_Identity=57.6271186440678, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=29.1196388261851, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=440, Percent_Identity=28.8636363636364, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564042, Length=433, Percent_Identity=29.3302540415704, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=24.5412844036697, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321559, Length=430, Percent_Identity=33.953488372093, Blast_Score=231, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6325396, Length=419, Percent_Identity=35.5608591885441, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324187, Length=426, Percent_Identity=33.5680751173709, Blast_Score=216, Evalue=7e-57,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=26.303317535545, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50769; Mature: 50769

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSNSCHFSDMKNGFFAICIFIALALLAGGNAIGRAEAQVQTNPLPSEQVTTELLMSEAT
CCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCC
PSDAVAIPKTTSPPVVDKGDVAWMLTSTLLVLLMVLPGLALFYGGMLRSKNVLSMMVQIG
CCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VVFSLIAILWVLYGYSLAFADGDMFIGTFNKAFLYGVDTATLVDTFSKGFKLPEYVYVAF
HHHHHHHHHHHHHCCHHEEECCCEEEEECCCEEEEECCHHHHHHHHHHCCCCCHHEEHHH
QLTFAGITAALIVGAFAERVKFAAVLLFIFIWFSFGYLPLAHMVWSTPGFLLSKGALDFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
GGTVVHINAGIAGLIGAYFVGKRIGFGQVAVKPHNVPMTFTGAALLWVGWFGFNAGSALE
CCEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCEEECHHHHHHHHHHCCCCCCCCC
ANSIAALAFINTMLATAAAILVWSLLEKMTKGKFSALGVASGMIAGLVGVTPAAGTVGPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
GAIAIGAVAAVCCIWGITGLKRLLKVDDTLDVFGVHGIGGIVGAILTGVFTDKALGGQGY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
AEGMTMSNQVMIQAMSVGISIVWIGIVSLVGFLIVKLLFGLRVSEEAEREGLDITSHGES
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
AYES
CCCC
>Mature Secondary Structure
MHSNSCHFSDMKNGFFAICIFIALALLAGGNAIGRAEAQVQTNPLPSEQVTTELLMSEAT
CCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCC
PSDAVAIPKTTSPPVVDKGDVAWMLTSTLLVLLMVLPGLALFYGGMLRSKNVLSMMVQIG
CCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
VVFSLIAILWVLYGYSLAFADGDMFIGTFNKAFLYGVDTATLVDTFSKGFKLPEYVYVAF
HHHHHHHHHHHHHCCHHEEECCCEEEEECCCEEEEECCHHHHHHHHHHCCCCCHHEEHHH
QLTFAGITAALIVGAFAERVKFAAVLLFIFIWFSFGYLPLAHMVWSTPGFLLSKGALDFA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCC
GGTVVHINAGIAGLIGAYFVGKRIGFGQVAVKPHNVPMTFTGAALLWVGWFGFNAGSALE
CCEEEEECCCHHHHHHHHHHHCCCCCCCEEECCCCCCEEECHHHHHHHHHHCCCCCCCCC
ANSIAALAFINTMLATAAAILVWSLLEKMTKGKFSALGVASGMIAGLVGVTPAAGTVGPF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCH
GAIAIGAVAAVCCIWGITGLKRLLKVDDTLDVFGVHGIGGIVGAILTGVFTDKALGGQGY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCC
AEGMTMSNQVMIQAMSVGISIVWIGIVSLVGFLIVKLLFGLRVSEEAEREGLDITSHGES
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
AYES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]