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The map label for this gene is lipA3

Identifier: 62185503

GI number: 62185503

Start: 1057390

End: 1058322

Strand: Direct

Name: lipA3

Synonym: CAB903

Alternate gene names: 62185503

Gene position: 1057390-1058322 (Clockwise)

Preceding gene: 62185502

Following gene: 62185504

Centisome position: 92.4

GC content: 42.77

Gene sequence:

>933_bases
ATGAATGACACTCCTAATGACATACAAAAACCCAAACAAGGGAAACGTTTTTCAGAACGTCTCCCTCACTGGCTAAGGCA
AGCTTTACCCAAAGGCTCAGTCTTTGACTTTACCGACAAAACTATAAAACGTACTGGCATGGCTACAGTATGTGAGGAAG
CGCTCTGCCCTAATCGTACGCGCTGTTGGTCAAGAAAGACAGCAACATACTTAGCTTTAGGAGATGCCTGCTCTCGACGT
TGTGGATTTTGTAACATTGATTTCACAAAAAAACCCCTACCTCCAGATCCTGATGAACCAAGAAAAATAGCAGAATCCGC
AAAGATCCTTCAGCTTAAGCATATCGTATTAACTATGGTCGCTCGCGACGACTTAGAAGATGGAGGGGCGAGTTGTTTGG
TACGTATTATCGATACTCTTCACAAAGAGCTCCCCGAATCTACTGTAGAAATGCTTGCTTCCGACTTTCAAGGAAATGTC
GATGCCTTGCATACCCTGCTAGATTCAGGATTAACCATTTATAATCACAACGTGGAAACAGTTGAACGTCTCACCCCTGT
AGTTCGACATAAAGCCACATACCGGCGTTCTTTATTTATGTTGGAGCAAGCCGCGTTATACCTCCCCGATCTTAAAATCA
AATCCGGCATCATGGTGGGGTTAGGAGAACAAGAAAGCGAGGTAAAACAAACACTCAAAGATCTTGCAGATCATGGTGTA
CGTATTGTCACAATAGGACAGTATTTACGTCCTTCACGATTGCACATACCTGTAAAGAGCTACGTTACTCCTGAGACTTT
TGATTACTACCGGAGAGTAGGAGAATCCTTAGGCCTCTTTGTTTATGCAGGGCCTTTCGTAAGATCCAGCTTCAACGCAG
ATATTGTTTTACAAGATATGGAAAATAAACAATCTAAAATGGCAAAAACGTAG

Upstream 100 bases:

>100_bases
TCTATGAAACTATACACGCTCATCCAACACTTGCTGAGGTTTGGGCTGAGAGTGCGTTATTAGCAACAAATCACCCTCTT
CATTTACCTCCTCCAAAACT

Downstream 100 bases:

>100_bases
AACTCCTGTGCATTTTCGCTGTCACTTTTTTAAAAGCACCTGTGTGTTAACGAGGCTTTTTTAGATCTAAATTTGTACGA
CTATAACTGCATATTAAAGA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNDTPNDIQKPKQGKRFSERLPHWLRQALPKGSVFDFTDKTIKRTGMATVCEEALCPNRTRCWSRKTATYLALGDACSRR
CGFCNIDFTKKPLPPDPDEPRKIAESAKILQLKHIVLTMVARDDLEDGGASCLVRIIDTLHKELPESTVEMLASDFQGNV
DALHTLLDSGLTIYNHNVETVERLTPVVRHKATYRRSLFMLEQAALYLPDLKIKSGIMVGLGEQESEVKQTLKDLADHGV
RIVTIGQYLRPSRLHIPVKSYVTPETFDYYRRVGESLGLFVYAGPFVRSSFNADIVLQDMENKQSKMAKT

Sequences:

>Translated_310_residues
MNDTPNDIQKPKQGKRFSERLPHWLRQALPKGSVFDFTDKTIKRTGMATVCEEALCPNRTRCWSRKTATYLALGDACSRR
CGFCNIDFTKKPLPPDPDEPRKIAESAKILQLKHIVLTMVARDDLEDGGASCLVRIIDTLHKELPESTVEMLASDFQGNV
DALHTLLDSGLTIYNHNVETVERLTPVVRHKATYRRSLFMLEQAALYLPDLKIKSGIMVGLGEQESEVKQTLKDLADHGV
RIVTIGQYLRPSRLHIPVKSYVTPETFDYYRRVGESLGLFVYAGPFVRSSFNADIVLQDMENKQSKMAKT
>Mature_310_residues
MNDTPNDIQKPKQGKRFSERLPHWLRQALPKGSVFDFTDKTIKRTGMATVCEEALCPNRTRCWSRKTATYLALGDACSRR
CGFCNIDFTKKPLPPDPDEPRKIAESAKILQLKHIVLTMVARDDLEDGGASCLVRIIDTLHKELPESTVEMLASDFQGNV
DALHTLLDSGLTIYNHNVETVERLTPVVRHKATYRRSLFMLEQAALYLPDLKIKSGIMVGLGEQESEVKQTLKDLADHGV
RIVTIGQYLRPSRLHIPVKSYVTPETFDYYRRVGESLGLFVYAGPFVRSSFNADIVLQDMENKQSKMAKT

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=291, Percent_Identity=39.8625429553265, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI37577164, Length=255, Percent_Identity=38.8235294117647, Blast_Score=187, Evalue=1e-47,
Organism=Escherichia coli, GI1786846, Length=288, Percent_Identity=40.9722222222222, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI32564533, Length=288, Percent_Identity=42.3611111111111, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324770, Length=293, Percent_Identity=43.3447098976109, Blast_Score=258, Evalue=8e-70,
Organism=Drosophila melanogaster, GI221513272, Length=308, Percent_Identity=40.2597402597403, Blast_Score=239, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_CHLAB (Q5L4V4)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220288.1
- ProteinModelPortal:   Q5L4V4
- SMR:   Q5L4V4
- GeneID:   3337451
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB903
- HOGENOM:   HBG284542
- OMA:   ARCPNIT
- PhylomeDB:   Q5L4V4
- ProtClustDB:   PRK05481
- BioCyc:   CABO218497:CAB903-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35057; Mature: 35057

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTPNDIQKPKQGKRFSERLPHWLRQALPKGSVFDFTDKTIKRTGMATVCEEALCPNRT
CCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCH
RCWSRKTATYLALGDACSRRCGFCNIDFTKKPLPPDPDEPRKIAESAKILQLKHIVLTMV
HHHCCCCHHHEEECHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ARDDLEDGGASCLVRIIDTLHKELPESTVEMLASDFQGNVDALHTLLDSGLTIYNHNVET
HHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHHH
VERLTPVVRHKATYRRSLFMLEQAALYLPDLKIKSGIMVGLGEQESEVKQTLKDLADHGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCE
RIVTIGQYLRPSRLHIPVKSYVTPETFDYYRRVGESLGLFVYAGPFVRSSFNADIVLQDM
EEEEECCCCCCCCEECCHHHCCCCHHHHHHHHHHHHCCEEEEECHHHHCCCCCCEEEECH
ENKQSKMAKT
HHHHHHHCCC
>Mature Secondary Structure
MNDTPNDIQKPKQGKRFSERLPHWLRQALPKGSVFDFTDKTIKRTGMATVCEEALCPNRT
CCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCH
RCWSRKTATYLALGDACSRRCGFCNIDFTKKPLPPDPDEPRKIAESAKILQLKHIVLTMV
HHHCCCCHHHEEECHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ARDDLEDGGASCLVRIIDTLHKELPESTVEMLASDFQGNVDALHTLLDSGLTIYNHNVET
HHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCHHH
VERLTPVVRHKATYRRSLFMLEQAALYLPDLKIKSGIMVGLGEQESEVKQTLKDLADHGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCE
RIVTIGQYLRPSRLHIPVKSYVTPETFDYYRRVGESLGLFVYAGPFVRSSFNADIVLQDM
EEEEECCCCCCCCEECCHHHCCCCHHHHHHHHHHHHCCEEEEECHHHHCCCCCCEEEECH
ENKQSKMAKT
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA