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The map label for this gene is bioF [H]

Identifier: 62185299

GI number: 62185299

Start: 796657

End: 797805

Strand: Direct

Name: bioF [H]

Synonym: CAB686

Alternate gene names: 62185299

Gene position: 796657-797805 (Clockwise)

Preceding gene: 62185298

Following gene: 62185300

Centisome position: 69.61

GC content: 39.69

Gene sequence:

>1149_bases
ATGCCAATCTGTGACCTGTTAGAACAAAGATTAGAAAAACAAAAACAGAAAAAGATTCACCGCACATTAAAAACTGTCTC
TGCTTCTATAGATTTCACGTCTAATGATTATCTTGGCTTTGCTAAATCTTTAGAGTTAAAACAAAGATTTTCTCATGCAT
GGGATTGTATGCATTCCCTGGGTTCTACAGGATCGCGTCTCCTCACAGGGCAATCTCTCCTAATAAATCGTGTAGAACAC
GACATTGCTGACTATCATCATATGGAAAGTGCTTTAGTTTTTAATTCTGGATACATGGCCAATCTCGGATTAATTTCAGC
ACTAGTTTCTAAAGAAGACCGTGTAATCTACGATATCTATATCCACGCTTCTATCCATGATGGCATTCGCCTTGCGCAAG
CCCAGGGGATCCCTTTCCGTCATAATGATATGCAACATTTAGAAAAGCGTTTATCACAACCGCACGGTGGACAAACCTTT
GTTTGCGTAGAATCGGTATATTCTCTTCATGGTTCAGTATCTCCCCTAAAAACTATCTGTGCTTTATGTAAAAAATACTC
TGCGCATCTTCTTGTTGATGAGGCACATGCTCTAGGAGTCTTTGGCAATATAGGGGAGGGATTCGTCACTTCTTTAGGGC
TTCAAAATGACGTATCTGCAACCGTATATACTTTTGGAAAAGCTTTAGGGATTCACGGTGCTGCAGTTGCCGGAAAAGCT
CTGCTTAAAGACTACCTCATTAATTTCTCTCGTCCCTTTATTTATACCACGGCTCTGCCTCCCCATGCGTTTGCATTGAT
CCAATTAGCCTATCAATATAACGAAAAAGCGCATGTCCTTAGAGAAAATCTCCAACAACTGATCTCTTATTTCCGAGCAA
AAGCACAAGACATGGGGTTACCTATGTTTAAAGACAATAGCAAGACGCCGATTCAGTCCTTATGTATACCAGGAAGCACT
CAAGTACGCGAAGCAGCAAAAACACTGCAAGATTTTGGCTTTGATGTTCGTCCTATTGTCAGTCCCACTGTAAAACAAAA
AGAAGAATTATTACGGATCTGTCTTCACTCGTTTAATACCCAAAGTGAAATCACAGAATTCTTGAATAAATTATACGCAG
TTCAAAAAGGCTTATGCATATTATCATAG

Upstream 100 bases:

>100_bases
TCCTCACTGTAGATAACAACGATATGGATGCAGACACTGCCATGTTGAATTTACTTGGCATGCGCCACCGTCCATCATTT
TCAATGGAAAGAGGGCAACC

Downstream 100 bases:

>100_bases
CAGGAATTGATACAGACGTCGGCAAGACATTCGTTAGCGCCATCTTAACGGTCCTACTTCAAGCAGAATATTGGAAGCCT
ATACAATCTGGCTCCTTAAA

Product: putative 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MPICDLLEQRLEKQKQKKIHRTLKTVSASIDFTSNDYLGFAKSLELKQRFSHAWDCMHSLGSTGSRLLTGQSLLINRVEH
DIADYHHMESALVFNSGYMANLGLISALVSKEDRVIYDIYIHASIHDGIRLAQAQGIPFRHNDMQHLEKRLSQPHGGQTF
VCVESVYSLHGSVSPLKTICALCKKYSAHLLVDEAHALGVFGNIGEGFVTSLGLQNDVSATVYTFGKALGIHGAAVAGKA
LLKDYLINFSRPFIYTTALPPHAFALIQLAYQYNEKAHVLRENLQQLISYFRAKAQDMGLPMFKDNSKTPIQSLCIPGST
QVREAAKTLQDFGFDVRPIVSPTVKQKEELLRICLHSFNTQSEITEFLNKLYAVQKGLCILS

Sequences:

>Translated_382_residues
MPICDLLEQRLEKQKQKKIHRTLKTVSASIDFTSNDYLGFAKSLELKQRFSHAWDCMHSLGSTGSRLLTGQSLLINRVEH
DIADYHHMESALVFNSGYMANLGLISALVSKEDRVIYDIYIHASIHDGIRLAQAQGIPFRHNDMQHLEKRLSQPHGGQTF
VCVESVYSLHGSVSPLKTICALCKKYSAHLLVDEAHALGVFGNIGEGFVTSLGLQNDVSATVYTFGKALGIHGAAVAGKA
LLKDYLINFSRPFIYTTALPPHAFALIQLAYQYNEKAHVLRENLQQLISYFRAKAQDMGLPMFKDNSKTPIQSLCIPGST
QVREAAKTLQDFGFDVRPIVSPTVKQKEELLRICLHSFNTQSEITEFLNKLYAVQKGLCILS
>Mature_381_residues
PICDLLEQRLEKQKQKKIHRTLKTVSASIDFTSNDYLGFAKSLELKQRFSHAWDCMHSLGSTGSRLLTGQSLLINRVEHD
IADYHHMESALVFNSGYMANLGLISALVSKEDRVIYDIYIHASIHDGIRLAQAQGIPFRHNDMQHLEKRLSQPHGGQTFV
CVESVYSLHGSVSPLKTICALCKKYSAHLLVDEAHALGVFGNIGEGFVTSLGLQNDVSATVYTFGKALGIHGAAVAGKAL
LKDYLINFSRPFIYTTALPPHAFALIQLAYQYNEKAHVLRENLQQLISYFRAKAQDMGLPMFKDNSKTPIQSLCIPGSTQ
VREAAKTLQDFGFDVRPIVSPTVKQKEELLRICLHSFNTQSEITEFLNKLYAVQKGLCILS

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI83977440, Length=398, Percent_Identity=29.3969849246231, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI83977442, Length=398, Percent_Identity=29.3969849246231, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI83977444, Length=398, Percent_Identity=29.3969849246231, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI284448556, Length=356, Percent_Identity=27.8089887640449, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI7657118, Length=360, Percent_Identity=28.0555555555556, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4758668, Length=383, Percent_Identity=28.9817232375979, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4502025, Length=399, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI40316939, Length=399, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI119220554, Length=389, Percent_Identity=29.0488431876607, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI5454084, Length=254, Percent_Identity=24.0157480314961, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786993, Length=346, Percent_Identity=34.6820809248555, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1790046, Length=366, Percent_Identity=28.1420765027322, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71994529, Length=375, Percent_Identity=26.4, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32566772, Length=367, Percent_Identity=26.158038147139, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560914, Length=366, Percent_Identity=27.5956284153005, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=366, Percent_Identity=27.5956284153005, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71982625, Length=248, Percent_Identity=22.5806451612903, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982617, Length=248, Percent_Identity=22.5806451612903, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=350, Percent_Identity=32, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6320267, Length=376, Percent_Identity=27.1276595744681, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323954, Length=269, Percent_Identity=20.4460966542751, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24662918, Length=359, Percent_Identity=28.6908077994429, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17137420, Length=364, Percent_Identity=30.2197802197802, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17136286, Length=365, Percent_Identity=27.1232876712329, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24653280, Length=249, Percent_Identity=23.6947791164659, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653276, Length=249, Percent_Identity=23.6947791164659, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653278, Length=249, Percent_Identity=23.6947791164659, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 42639; Mature: 42507

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPICDLLEQRLEKQKQKKIHRTLKTVSASIDFTSNDYLGFAKSLELKQRFSHAWDCMHSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GSTGSRLLTGQSLLINRVEHDIADYHHMESALVFNSGYMANLGLISALVSKEDRVIYDIY
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCEEEEEE
IHASIHDGIRLAQAQGIPFRHNDMQHLEKRLSQPHGGQTFVCVESVYSLHGSVSPLKTIC
EEEEHHCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCHHHHHH
ALCKKYSAHLLVDEAHALGVFGNIGEGFVTSLGLQNDVSATVYTFGKALGIHGAAVAGKA
HHHHHCCCCEEEECHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
LLKDYLINFSRPFIYTTALPPHAFALIQLAYQYNEKAHVLRENLQQLISYFRAKAQDMGL
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PMFKDNSKTPIQSLCIPGSTQVREAAKTLQDFGFDVRPIVSPTVKQKEELLRICLHSFNT
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
QSEITEFLNKLYAVQKGLCILS
HHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure 
PICDLLEQRLEKQKQKKIHRTLKTVSASIDFTSNDYLGFAKSLELKQRFSHAWDCMHSL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GSTGSRLLTGQSLLINRVEHDIADYHHMESALVFNSGYMANLGLISALVSKEDRVIYDIY
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCEEEEEE
IHASIHDGIRLAQAQGIPFRHNDMQHLEKRLSQPHGGQTFVCVESVYSLHGSVSPLKTIC
EEEEHHCCHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCHHHHHH
ALCKKYSAHLLVDEAHALGVFGNIGEGFVTSLGLQNDVSATVYTFGKALGIHGAAVAGKA
HHHHHCCCCEEEECHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
LLKDYLINFSRPFIYTTALPPHAFALIQLAYQYNEKAHVLRENLQQLISYFRAKAQDMGL
HHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
PMFKDNSKTPIQSLCIPGSTQVREAAKTLQDFGFDVRPIVSPTVKQKEELLRICLHSFNT
CCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
QSEITEFLNKLYAVQKGLCILS
HHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2110099 [H]