Click here to switch to the map view.

The map label for this gene is uvrD [H]

Identifier: 28572404

GI number: 28572404

Start: 272079

End: 273836

Strand: Direct

Name: uvrD [H]

Synonym: TW245

Alternate gene names: 28572404

Gene position: 272079-273836 (Clockwise)

Preceding gene: 28572402

Following gene: 28572406

Centisome position: 29.38

GC content: 46.64

Gene sequence:

>1758_bases
TTGACAATTGATGTTTTTTCGGGACTTAGCGACCAACAGTGTGATGCTGTCCGTACCCTGAGGGGGCCTGTAAAAATAAT
TGCCGGCGCCGGAAGTGGCAAAACACTGACAATTACAAGAAGAATAGTCAATGGCATTATCTCCAATGAGTATGATTCCG
GGCATGTTATGGCTTTGAGTTTCACTCGCCGCGCGAGCATGCAACTCAGGAGTCGCCTTGTAGAGCACGGCGTTAAAGGG
GTTTATTCAGGCACTTTTCACAGCGCGGCACTTTCACAGCTCAGGTTTTTTTGGGAGACCTTTCACGGAAAGCCTCCAAG
ACAGATAATTCGCGATAAGGTGAATTTACTGCGGGACGCAGCACGAGGAATAAACTTTAGCTTTTCGGATGAGTCATTGT
GTCAGGTTGCTTCGGAAATAGAATGGGCAAAACAAAACGGCTACGACTTGAGTGGATACGATAAGTGCAATCACAGAATT
TCTGGTATGAGTGCCAGTCAGGCGCGCACTCTCATGCTTGCTTATGAGGATGTAAAGAACAATAGAAGGCTAATCGACTT
TGAGGATATACTTTCGCTCACAGTTGGCATGCTGTCTGTTCAGCCGAGAGCCGTTGCTCGTGTGCGAGACAGGTTTAGGG
TATTTTTGATTGATGAATTTCAGGATGTGTCACCCCTACAATTTGAGCTCCTGCGAATTTGGCTTGGGCGCAGGCGTGAT
ATTTGTGTTGTCGGAGATCCTAATCAGTGTATATATTCGTTTGCAGGGGCAAAAAGGGATTATCTTGTCAAATTTGCCGA
CTATTTTCCGGGCGCGCGAGAGGTTTTTCTTAGCCGTAATTACAGATCTCGTCCGGAAATAGTGTGTTTATCTAGGCACA
TTATTGACTCTCACTCACCGGATATCGGACTGTACCAGCCCACCGAGGGTATCTCTCGCAATTATGCCGATACAAAAACG
GCTACTCCTGCTCAGGATATGCGGGTAAATGCCGGAAATAGAGCGAAATGTCATTGCACGGCACCCCCTGGCACCAAAGG
CGCGGTGAGATGTGGTAATTCCGATTTGGGTTATCGTGAAACCCCTATTCGTTGGCATACTTTTGAAAACCAGATTCAGG
AAATGCATTTTATTGCTCAGAGTCTGAAGAATATTTCGCAGAGCTGTGCTGTGCTCTACAGAACGGCAATTCAGGGCGAG
CGGTTGGCAAAATATTTACACACATCAGGAATAATTTATAGAAGCCAAGAAAGCCGGCTGTTTCATGAAACTGATGTTGC
ACAGATTGCCTGCAAGCGGGTTATTGAAAAGACGATTGAATGTAACGTCAGGGTGGTTGATTTGGTTGATAGTGTTGTGT
CTGGAATATTCCCGCGCAAGCCAGATGCACTAACAGGCGAGTACGATGGTTGGGTTGCCCTTCAGGCATTGCGATTGTGC
GCTAGGCGTTTCAATGGCAATGCTTCGTCTTTTGCCACATATTTGCTCGAAATGCAGCGGCATAGGCGTGAGCCTGAAAT
GCCCTTCGCAACCCTCGCAACCTTACATTCAACAAAGGGTCTTGAGTGGGACACTGTATACATAATCGGTGTCAACGAAG
GTCTAATTCCACATCACACCGGTGACGAAGATGAGGAAAAACGCCTATTTTATGTGGGCGTTACCCGCGCGAGAAAAAAC
ATTGTATTCAGCAGCACACGCACTCCCAGTAGGTATTTAACTGAATTGTGCTTATCACCTATATCTGCCAAGGAATAG

Upstream 100 bases:

>100_bases
ATTGGTGAGAGCGCAATCATGACCCTTAAAAGGCTAAGACTTTTTTGTGAATTGAGGGCGCAGCCACGCAAATATTGGCA
ATGTTTTTGATGGGAAATAA

Downstream 100 bases:

>100_bases
GCATGCATGCCTGAATTTATATGCGCTATGTGCCTTGATTAGCTTTTACTTGCGTCCTTTCCGTTGCTATTTGTATCACC
TTCATCGTCACCTGTGCTAT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTIDVFSGLSDQQCDAVRTLRGPVKIIAGAGSGKTLTITRRIVNGIISNEYDSGHVMALSFTRRASMQLRSRLVEHGVKG
VYSGTFHSAALSQLRFFWETFHGKPPRQIIRDKVNLLRDAARGINFSFSDESLCQVASEIEWAKQNGYDLSGYDKCNHRI
SGMSASQARTLMLAYEDVKNNRRLIDFEDILSLTVGMLSVQPRAVARVRDRFRVFLIDEFQDVSPLQFELLRIWLGRRRD
ICVVGDPNQCIYSFAGAKRDYLVKFADYFPGAREVFLSRNYRSRPEIVCLSRHIIDSHSPDIGLYQPTEGISRNYADTKT
ATPAQDMRVNAGNRAKCHCTAPPGTKGAVRCGNSDLGYRETPIRWHTFENQIQEMHFIAQSLKNISQSCAVLYRTAIQGE
RLAKYLHTSGIIYRSQESRLFHETDVAQIACKRVIEKTIECNVRVVDLVDSVVSGIFPRKPDALTGEYDGWVALQALRLC
ARRFNGNASSFATYLLEMQRHRREPEMPFATLATLHSTKGLEWDTVYIIGVNEGLIPHHTGDEDEEKRLFYVGVTRARKN
IVFSSTRTPSRYLTELCLSPISAKE

Sequences:

>Translated_585_residues
MTIDVFSGLSDQQCDAVRTLRGPVKIIAGAGSGKTLTITRRIVNGIISNEYDSGHVMALSFTRRASMQLRSRLVEHGVKG
VYSGTFHSAALSQLRFFWETFHGKPPRQIIRDKVNLLRDAARGINFSFSDESLCQVASEIEWAKQNGYDLSGYDKCNHRI
SGMSASQARTLMLAYEDVKNNRRLIDFEDILSLTVGMLSVQPRAVARVRDRFRVFLIDEFQDVSPLQFELLRIWLGRRRD
ICVVGDPNQCIYSFAGAKRDYLVKFADYFPGAREVFLSRNYRSRPEIVCLSRHIIDSHSPDIGLYQPTEGISRNYADTKT
ATPAQDMRVNAGNRAKCHCTAPPGTKGAVRCGNSDLGYRETPIRWHTFENQIQEMHFIAQSLKNISQSCAVLYRTAIQGE
RLAKYLHTSGIIYRSQESRLFHETDVAQIACKRVIEKTIECNVRVVDLVDSVVSGIFPRKPDALTGEYDGWVALQALRLC
ARRFNGNASSFATYLLEMQRHRREPEMPFATLATLHSTKGLEWDTVYIIGVNEGLIPHHTGDEDEEKRLFYVGVTRARKN
IVFSSTRTPSRYLTELCLSPISAKE
>Mature_584_residues
TIDVFSGLSDQQCDAVRTLRGPVKIIAGAGSGKTLTITRRIVNGIISNEYDSGHVMALSFTRRASMQLRSRLVEHGVKGV
YSGTFHSAALSQLRFFWETFHGKPPRQIIRDKVNLLRDAARGINFSFSDESLCQVASEIEWAKQNGYDLSGYDKCNHRIS
GMSASQARTLMLAYEDVKNNRRLIDFEDILSLTVGMLSVQPRAVARVRDRFRVFLIDEFQDVSPLQFELLRIWLGRRRDI
CVVGDPNQCIYSFAGAKRDYLVKFADYFPGAREVFLSRNYRSRPEIVCLSRHIIDSHSPDIGLYQPTEGISRNYADTKTA
TPAQDMRVNAGNRAKCHCTAPPGTKGAVRCGNSDLGYRETPIRWHTFENQIQEMHFIAQSLKNISQSCAVLYRTAIQGER
LAKYLHTSGIIYRSQESRLFHETDVAQIACKRVIEKTIECNVRVVDLVDSVVSGIFPRKPDALTGEYDGWVALQALRLCA
RRFNGNASSFATYLLEMQRHRREPEMPFATLATLHSTKGLEWDTVYIIGVNEGLIPHHTGDEDEEKRLFYVGVTRARKNI
VFSSTRTPSRYLTELCLSPISAKE

Specific function: Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=313, Percent_Identity=28.1150159744409, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI48994965, Length=307, Percent_Identity=28.6644951140065, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR002121
- InterPro:   IPR014016
- InterPro:   IPR010997 [H]

Pfam domain/function: PF00570 HRDC; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 66217; Mature: 66086

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDVFSGLSDQQCDAVRTLRGPVKIIAGAGSGKTLTITRRIVNGIISNEYDSGHVMALS
CEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCCCCEEEEE
FTRRASMQLRSRLVEHGVKGVYSGTFHSAALSQLRFFWETFHGKPPRQIIRDKVNLLRDA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ARGINFSFSDESLCQVASEIEWAKQNGYDLSGYDKCNHRISGMSASQARTLMLAYEDVKN
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHCCCCHHHHHEEEEEEHHHCC
NRRLIDFEDILSLTVGMLSVQPRAVARVRDRFRVFLIDEFQDVSPLQFELLRIWLGRRRD
CCEEECHHHHHHHHHHHHCCCHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ICVVGDPNQCIYSFAGAKRDYLVKFADYFPGAREVFLSRNYRSRPEIVCLSRHIIDSHSP
EEEECCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEHHHHCCCCC
DIGLYQPTEGISRNYADTKTATPAQDMRVNAGNRAKCHCTAPPGTKGAVRCGNSDLGYRE
CCCCCCCCCCCCCCCCCCCCCCCHHHCEECCCCCCEEEECCCCCCCCEEEECCCCCCCCC
TPIRWHTFENQIQEMHFIAQSLKNISQSCAVLYRTAIQGERLAKYLHTSGIIYRSQESRL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCH
FHETDVAQIACKRVIEKTIECNVRVVDLVDSVVSGIFPRKPDALTGEYDGWVALQALRLC
HHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
ARRFNGNASSFATYLLEMQRHRREPEMPFATLATLHSTKGLEWDTVYIIGVNEGLIPHHT
HHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCC
GDEDEEKRLFYVGVTRARKNIVFSSTRTPSRYLTELCLSPISAKE
CCCCCCCEEEEEEEEHHHCCEEEECCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TIDVFSGLSDQQCDAVRTLRGPVKIIAGAGSGKTLTITRRIVNGIISNEYDSGHVMALS
EEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCEEEEEHHHHHHHHCCCCCCCCEEEEE
FTRRASMQLRSRLVEHGVKGVYSGTFHSAALSQLRFFWETFHGKPPRQIIRDKVNLLRDA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ARGINFSFSDESLCQVASEIEWAKQNGYDLSGYDKCNHRISGMSASQARTLMLAYEDVKN
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHCCCCHHHHHEEEEEEHHHCC
NRRLIDFEDILSLTVGMLSVQPRAVARVRDRFRVFLIDEFQDVSPLQFELLRIWLGRRRD
CCEEECHHHHHHHHHHHHCCCHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCC
ICVVGDPNQCIYSFAGAKRDYLVKFADYFPGAREVFLSRNYRSRPEIVCLSRHIIDSHSP
EEEECCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEHHHHCCCCC
DIGLYQPTEGISRNYADTKTATPAQDMRVNAGNRAKCHCTAPPGTKGAVRCGNSDLGYRE
CCCCCCCCCCCCCCCCCCCCCCCHHHCEECCCCCCEEEECCCCCCCCEEEECCCCCCCCC
TPIRWHTFENQIQEMHFIAQSLKNISQSCAVLYRTAIQGERLAKYLHTSGIIYRSQESRL
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCH
FHETDVAQIACKRVIEKTIECNVRVVDLVDSVVSGIFPRKPDALTGEYDGWVALQALRLC
HHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
ARRFNGNASSFATYLLEMQRHRREPEMPFATLATLHSTKGLEWDTVYIIGVNEGLIPHHT
HHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCC
GDEDEEKRLFYVGVTRARKNIVFSSTRTPSRYLTELCLSPISAKE
CCCCCCCEEEEEEEEHHHCCEEEECCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]