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The map label for this gene is tyrS [H]

Identifier: 23501810

GI number: 23501810

Start: 898790

End: 900043

Strand: Reverse

Name: tyrS [H]

Synonym: BR0926

Alternate gene names: 23501810

Gene position: 900043-898790 (Counterclockwise)

Preceding gene: 23501812

Following gene: 23501808

Centisome position: 42.7

GC content: 58.29

Gene sequence:

>1254_bases
ATGTCCGGTTTCAAATCCGATTTTCTTCGCACGCTTTCCGAACGTGGCTTCATCCATCAGATTTCCGATGAAAGCGGCCT
CGACGAATTGTTGGCCAAGGAGACCGTGACGGCCTATATCGGCTTTGATCCCACGGCGCCCAGCCTCCATGCCGGCGGCC
TGATCCAGATCATGATGCTCTATTGGCTCCAGCAGACCGGCCACAAGCCGGTGGCGCTGATGGGCGGCGGCACGGGCATG
GTGGGCGATCCGTCCTTCAAGGACGAAGCGCGCAAACTCATGACCGAGGATACGATTGCCGAAAACATGGCGAGCATCAA
GCGCGTCTTCGCCAATTACCTCACCTTTGGTGACGGAGCGAACGATGCGCTGATGGTCAACAATGGCGAGTGGCTGCGCA
ATATCAATTACCTGGAATTCCTGCGCGATGTGGGGCGGCATTTTTCGGTCAACCGGATGCTCTCCTTCGATTCGGTGAAG
TTGCGCCTCGACCGTGAGCAGTCGCTTTCCTTCCTCGAATTCAACTACATGATCCTTCAGGCCTACGACTTCGTGGAACT
GAACAAGCGTTACGGCCTGCGCCTACAAATGGGCGGCTCGGACCAATGGGGCAATATTGTCAATGGTATTGACCTCGGCC
ACCGGATGGGCACACCGCAGCTTTACGCCCTCACCTCGCCGCTCCTGACCACTGCGTCCGGCCAGAAAATGGGCAAGTCG
CTTGGCGGCGCCATCTGGCTCAATGCAGATATGCTCAGTGCCTATGACTTCTGGCAATATTGGCGCAATACGGAAGACGC
GGACGTGGAACGCTTCCTCAAGCTCTACACGACGCTGCCGCTGGACGAGATCGCAAGGCTCGCAGAACTCGGCGGCGCGG
AAATCAACGAAGCCAAGAAGATACTGGCGACCGAAGTGACCGCCATGCTGCATGGGCGCGATGCCGCCGAAGAAGCAGCC
GAAACCGCACGCAAGACCTTTGAAGACGGCGAACTTTCGGAAAACCTGCCCACAGTCGGCGTGCACAAGGCGACGCTTAA
TGACGGGATCGGTGTTCTGGCGCTGATGGTTCTGGCCGAACTTTGCACCACCAACGGCGAAGCACGCCGCCATGTGGAAG
GCGGGGCGGTGCGCATCAACGATGAGCCGGTCAGCGATCCGCGCATGGTTGTCAATGCTGCAGCACTAAACGGTCAGGGC
CTCATCAAGCTGTCGCTCGGCAAGAAACGCCATGTTCTGATCCGCCCCGCATAA

Upstream 100 bases:

>100_bases
TTATTCCCGGTTGATTGATGCTAAAGGCGGACATATCACAAGGCAGTAAGTACTTGCTTACCGTCAATTTCAATTTCGCC
TCATCAGTCAGGAAAAGGGC

Downstream 100 bases:

>100_bases
AGCGGCGGAATAAAGCATCAAAAAACCGGGCCTCGCGCCCGGTTTTGTTTATCGGGCCTTATTGGAACTCGAAGATGGAA
CGGAAAATGCCCGGCGCGAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MSGFKSDFLRTLSERGFIHQISDESGLDELLAKETVTAYIGFDPTAPSLHAGGLIQIMMLYWLQQTGHKPVALMGGGTGM
VGDPSFKDEARKLMTEDTIAENMASIKRVFANYLTFGDGANDALMVNNGEWLRNINYLEFLRDVGRHFSVNRMLSFDSVK
LRLDREQSLSFLEFNYMILQAYDFVELNKRYGLRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGQKMGKS
LGGAIWLNADMLSAYDFWQYWRNTEDADVERFLKLYTTLPLDEIARLAELGGAEINEAKKILATEVTAMLHGRDAAEEAA
ETARKTFEDGELSENLPTVGVHKATLNDGIGVLALMVLAELCTTNGEARRHVEGGAVRINDEPVSDPRMVVNAAALNGQG
LIKLSLGKKRHVLIRPA

Sequences:

>Translated_417_residues
MSGFKSDFLRTLSERGFIHQISDESGLDELLAKETVTAYIGFDPTAPSLHAGGLIQIMMLYWLQQTGHKPVALMGGGTGM
VGDPSFKDEARKLMTEDTIAENMASIKRVFANYLTFGDGANDALMVNNGEWLRNINYLEFLRDVGRHFSVNRMLSFDSVK
LRLDREQSLSFLEFNYMILQAYDFVELNKRYGLRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGQKMGKS
LGGAIWLNADMLSAYDFWQYWRNTEDADVERFLKLYTTLPLDEIARLAELGGAEINEAKKILATEVTAMLHGRDAAEEAA
ETARKTFEDGELSENLPTVGVHKATLNDGIGVLALMVLAELCTTNGEARRHVEGGAVRINDEPVSDPRMVVNAAALNGQG
LIKLSLGKKRHVLIRPA
>Mature_416_residues
SGFKSDFLRTLSERGFIHQISDESGLDELLAKETVTAYIGFDPTAPSLHAGGLIQIMMLYWLQQTGHKPVALMGGGTGMV
GDPSFKDEARKLMTEDTIAENMASIKRVFANYLTFGDGANDALMVNNGEWLRNINYLEFLRDVGRHFSVNRMLSFDSVKL
RLDREQSLSFLEFNYMILQAYDFVELNKRYGLRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGQKMGKSL
GGAIWLNADMLSAYDFWQYWRNTEDADVERFLKLYTTLPLDEIARLAELGGAEINEAKKILATEVTAMLHGRDAAEEAAE
TARKTFEDGELSENLPTVGVHKATLNDGIGVLALMVLAELCTTNGEARRHVEGGAVRINDEPVSDPRMVVNAAALNGQGL
IKLSLGKKRHVLIRPA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=429, Percent_Identity=34.2657342657343, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1787925, Length=419, Percent_Identity=44.1527446300716, Blast_Score=352, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI25145802, Length=403, Percent_Identity=30.5210918114144, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=322, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=35.6164383561644, Blast_Score=235, Evalue=8e-63,
Organism=Drosophila melanogaster, GI20130383, Length=432, Percent_Identity=32.4074074074074, Blast_Score=241, Evalue=9e-64,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 46099; Mature: 45968

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFKSDFLRTLSERGFIHQISDESGLDELLAKETVTAYIGFDPTAPSLHAGGLIQIMML
CCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHH
YWLQQTGHKPVALMGGGTGMVGDPSFKDEARKLMTEDTIAENMASIKRVFANYLTFGDGA
HHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NDALMVNNGEWLRNINYLEFLRDVGRHFSVNRMLSFDSVKLRLDREQSLSFLEFNYMILQ
CCEEEEECCHHHHCCCHHHHHHHHHHCCCHHHEEECCCEEEEECHHHCCCHHHHHHHEEE
AYDFVELNKRYGLRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGQKMGKS
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHH
LGGAIWLNADMLSAYDFWQYWRNTEDADVERFLKLYTTLPLDEIARLAELGGAEINEAKK
CCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
ILATEVTAMLHGRDAAEEAAETARKTFEDGELSENLPTVGVHKATLNDGIGVLALMVLAE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHH
LCTTNGEARRHVEGGAVRINDEPVSDPRMVVNAAALNGQGLIKLSLGKKRHVLIRPA
HHHCCCHHHEECCCCEEEECCCCCCCCHHEEEEEEECCCCEEEEECCCCEEEEEECC
>Mature Secondary Structure 
SGFKSDFLRTLSERGFIHQISDESGLDELLAKETVTAYIGFDPTAPSLHAGGLIQIMML
CCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHH
YWLQQTGHKPVALMGGGTGMVGDPSFKDEARKLMTEDTIAENMASIKRVFANYLTFGDGA
HHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NDALMVNNGEWLRNINYLEFLRDVGRHFSVNRMLSFDSVKLRLDREQSLSFLEFNYMILQ
CCEEEEECCHHHHCCCHHHHHHHHHHCCCHHHEEECCCEEEEECHHHCCCHHHHHHHEEE
AYDFVELNKRYGLRLQMGGSDQWGNIVNGIDLGHRMGTPQLYALTSPLLTTASGQKMGKS
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEECCCHHHCCCCHHHHHH
LGGAIWLNADMLSAYDFWQYWRNTEDADVERFLKLYTTLPLDEIARLAELGGAEINEAKK
CCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
ILATEVTAMLHGRDAAEEAAETARKTFEDGELSENLPTVGVHKATLNDGIGVLALMVLAE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHCCCHHHHHHHHHHH
LCTTNGEARRHVEGGAVRINDEPVSDPRMVVNAAALNGQGLIKLSLGKKRHVLIRPA
HHHCCCHHHEECCCCEEEECCCCCCCCHHEEEEEEECCCCEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA