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The map label for this gene is rne [H]

Identifier: 23501798

GI number: 23501798

Start: 883373

End: 886141

Strand: Reverse

Name: rne [H]

Synonym: BR0912

Alternate gene names: 23501798

Gene position: 886141-883373 (Counterclockwise)

Preceding gene: 23501800

Following gene: 23501796

Centisome position: 42.04

GC content: 58.9

Gene sequence:

>2769_bases
ATGTCCAACAAAATGCTTATAGATGCCTCCCACCCGGAGGAGACCCGCGTTATCGTCACGCGCGGCAACAAGATTGAAGA
ATTCGATTTCGAATCGGAACATAAGAAACAGCTGAAAGGCAATATCTACCTCGCACGCGTCACCCGTGTCGAACCGTCGC
TTCAGGCTGCATTCGTGGAATATGGCGGCAATCGCCATGGCTTCCTGGCATTTTCCGAAATTCATCCCGACTATTACCAG
ATTCCGGTCGCCGACCGCCTTGCCCTGCTTGAAGCGGAAGCCAAGGCCGCACGCGCTGAAGACGACGAGGACGAACCGCG
CGCCAAAAGCGACCGCAGTGAACGCAACGACCGCGCGCGCCGCAATCGCCGCCGTGGCGGCCGCCGCGATGGCCAGAATG
GCGAAGCGCAGGCAAACGGTGCTGACAAGGACCTGCCGGCGGCAGAGCCGGTCGGTCCGGGCTTTGCGGATTACGTTGCG
GGAACGCCCGCCGCTGCTCTCACGCAGAAAGGCGATATAATTTCCGAAGCCCTCGACAATGGCGATGACGATGATGTCGA
GAACGAAGAGGACATGGTCGAATCGGTCGGCTCGGAAGACGCTCTGGAAGAGCTTCCGACCCACACCCGCACCCATCGCC
GCCAGTATAATATTCAGGAAGTCATCAAGCGCCGCCAGATTCTTCTGGTGCAGGTGGTCAAGGAAGAGCGCGGCAACAAG
GGCGCTGCTCTCACCACCTATCTTTCGCTTGCCGGACGTTATTCGGTACTGATGCCCAACACCGCGCGCGGTGGCGGCAT
TTCGCGCAAGATCACCAATCCGCAGGACCGCAAGCGCCTGAAAGAGATCGTGAAGGAACTCGAAGTGCCGCAGGGCATGA
GTGTGATCCTGCGCACGGCAGGCGCAAACCGCACCAAGGCGGAAGTCAAGCGCGACTATGAATATCTGATGCGCCTGTGG
GAAAACGTGCGCACGCTGACGCTGCAATCGACAGCGCCCGCGCTCGTCTATGAAGAAGGCAGCCTCATCAAGCGCTCTAT
CCGTGATCTTTACAACAAGGACATCACGGAAATACTCGTTTCCGGCGAGAACGGCTATCGCGAGGCCAAGGATTTCATGC
GTATGCTCATGCCGAGCCATGCGAAGGTCGTCCAGCCTTACCGCGATCATATTCCGATCTTCACGCGCAACGGCGTGGAA
GCGCAGCTTGACCGTATGCTTGTGCCGCAGGTGACGCTCAAATCGGGCGGCTACCTCATCATCAACCAGACGGAAGCCCT
GGTTGCCATCGACGTCAATTCGGGCCGCTCGACCCGCGAACATTCGATTGAGGACACCGCGCTTCAGACCAATCTGGAAG
CAGCCGAAGAAGTTGCGCGCCAGTTGCGCCTTCGCGACCTTGCGGGCCTGATCGTCGTTGACTTCATCGACATGGAAGAA
AAGCGCAACAATCGCGCTGTCGAAAAGAAGATGAAGGAATGCCTGAAGGATGACCGCGCCCGCATCCAGGTGGGCCGCAT
CTCGCATTTCGGCCTTCTGGAAATGTCGCGCCAGCGCATCCGCGCATCGGTTCTGGAAAGCACCATGCAGGTCTGCCCGC
ATTGCGGCGGCACCGGACATATCCGTTCGGATTCCTCCATGGCGCTCCATATCATCCGTGGTATCGAAGACTATCTGCTG
CGCCATTCGGGCTTTGATATTCATGTCCGCACCCCGGCAGCGTCCGCACTTTATGTGCTCAATCACAAGCGCCAGATTCT
GGCCGATCTGGAGGGGCGCTTTGGCGTCGAGATCAGCATTGATGCCGACGAAAGTGTAGGAAACCAGCACTTTGTCATTG
ATAAGGGTGCGCCTTCTACCCGCCCCGTCACCCCGTCGGCAGTACAGCCGATGGTCTATGAGGACGATATCGAAGACCCT
GAAATCCCGGTCGAAGAAGACGAGACCGAAGAAGAAGCAAGGACCGATGTTCAGGCTGGCGAAGAGCGCAGCGAAGAAAA
CGACCGCAAGCGCCGCCGTCGTCGCCGTCGTCGCGGTGGCCGCGAACGTGACGGTCGTGATCTGTCCGCGCAGGAAGAAG
CCGTTTCGGAGGAAGCATCCGAAGAGGATAATGAAACCGATCAGGCCAATGAGGCCGTGTCATCCGCTTCCATGAGCGAG
GAAGATCGCCGCAAGAAGCGTCGTCGCGGCCGCCGTGGCGGACGGAAGAACCGGCGTGAAGACGACAACCGTGCGCGCAG
AATTCCTGATCCGGAATTCGTCGGTTACACACCTGTTCTGCCGGTAAAGGCCGAAGAAAGCATCGGCGAAGTAGTGGAAA
CGGTTGAAACCGTGGAAGCACCTGTCGTTGAGGCCGCGCCGGAAGAAAAGCCTGAAAAGCCCGCCCGCAAGCCGCGTGCC
CGCAAAACAGCAAAAACCGAGGAAGCAGCGGAAGCAGTGATCACTGCGGAGGCTGAGGAAAAGCCTGAAAAGCCCGCCCG
CAAAACGCGCACACGCAAGAAGGCTGTCGACGAAGCGCCTGCAGATGAAGCCGCAACGCCCGCCGAGGAAGTGGTACCTG
AAAAGCCGAAGCGCCGTACACGCAAAGCTGCCGCCAAGCCGGTTGTGGAAGCCGAAGACGTTGCCGAGGTGAAGGAAACG
GTTGCGGCAGAAACGCAGGTGGAAACCATCGCGGAACCGAAAAGCACAGAGCCGGTCGTAACCTCCAGCGAGAGCGAAGA
CCGGAAGCCGAAGCGTTCGGGCTGGTGGCAGAAGAAGGGCTTTTTCTAA

Upstream 100 bases:

>100_bases
CCAGAATCATCCGGCAGATGCATCGCAGTCAGGACAAAAGCCTTCAGGGCTTCGTCCGGCGCACGCGGCTTGCGTCTTCG
CGCCGGCAGGAAAGACAATA

Downstream 100 bases:

>100_bases
GAAGCCGGGCCGCCTTCCCGCTGCCCTGTTACGGATGACAAAAAACCGGCGGAAACGCCGGTTTTTCTTTATGTTTTTGC
CGCAGAACGGCTCCCTTTCA

Product: ribonuclease Rne/Rng domain-containing protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 922; Mature: 921

Protein sequence:

>922_residues
MSNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
IPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRARRNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVA
GTPAAALTQKGDIISEALDNGDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK
GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMSVILRTAGANRTKAEVKRDYEYLMRLW
ENVRTLTLQSTAPALVYEEGSLIKRSIRDLYNKDITEILVSGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVE
AQLDRMLVPQVTLKSGGYLIINQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE
KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGHIRSDSSMALHIIRGIEDYLL
RHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISIDADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDP
EIPVEEDETEEEARTDVQAGEERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE
EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEAPVVEAAPEEKPEKPARKPRA
RKTAKTEEAAEAVITAEAEEKPEKPARKTRTRKKAVDEAPADEAATPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKET
VAAETQVETIAEPKSTEPVVTSSESEDRKPKRSGWWQKKGFF

Sequences:

>Translated_922_residues
MSNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
IPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRARRNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVA
GTPAAALTQKGDIISEALDNGDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK
GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMSVILRTAGANRTKAEVKRDYEYLMRLW
ENVRTLTLQSTAPALVYEEGSLIKRSIRDLYNKDITEILVSGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVE
AQLDRMLVPQVTLKSGGYLIINQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE
KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGHIRSDSSMALHIIRGIEDYLL
RHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISIDADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDP
EIPVEEDETEEEARTDVQAGEERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE
EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEAPVVEAAPEEKPEKPARKPRA
RKTAKTEEAAEAVITAEAEEKPEKPARKTRTRKKAVDEAPADEAATPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKET
VAAETQVETIAEPKSTEPVVTSSESEDRKPKRSGWWQKKGFF
>Mature_921_residues
SNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQI
PVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRARRNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVAG
TPAAALTQKGDIISEALDNGDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNKG
AALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMSVILRTAGANRTKAEVKRDYEYLMRLWE
NVRTLTLQSTAPALVYEEGSLIKRSIRDLYNKDITEILVSGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVEA
QLDRMLVPQVTLKSGGYLIINQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEEK
RNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGHIRSDSSMALHIIRGIEDYLLR
HSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISIDADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDPE
IPVEEDETEEEARTDVQAGEERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSEE
DRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEAPVVEAAPEEKPEKPARKPRAR
KTAKTEEAAEAVITAEAEEKPEKPARKTRTRKKAVDEAPADEAATPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKETV
AAETQVETIAEPKSTEPVVTSSESEDRKPKRSGWWQKKGFF

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=444, Percent_Identity=42.7927927927928, Blast_Score=358, Evalue=1e-99,
Organism=Escherichia coli, GI87082235, Length=339, Percent_Identity=37.1681415929204, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 103773; Mature: 103642

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVE
CCCCEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQIPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRAR
HCCCCCCEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
RNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVAGTPAAALTQKGDIISEALDN
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHCC
GDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK
CCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMSVILRTA
CCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
GANRTKAEVKRDYEYLMRLWENVRTLTLQSTAPALVYEEGSLIKRSIRDLYNKDITEILV
CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHEE
SGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVEAQLDRMLVPQVTLKSGGYLI
CCCCCHHHHHHHHHHHCCCHHHHHCCHHHCCCEEECCCCHHHHHHHHCCCEEECCCCEEE
INQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE
EECCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGH
HHCCHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IRSDSSMALHIIRGIEDYLLRHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISI
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
DADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDPEIPVEEDETEEEARTDVQAG
CCCCCCCCCEEEEECCCCCCCCCCHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCC
EERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEA
HHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
PVVEAAPEEKPEKPARKPRARKTAKTEEAAEAVITAEAEEKPEKPARKTRTRKKAVDEAP
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHCCC
ADEAATPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKETVAAETQVETIAEPKSTEPVV
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
TSSESEDRKPKRSGWWQKKGFF
ECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNKMLIDASHPEETRVIVTRGNKIEEFDFESEHKKQLKGNIYLARVTRVEPSLQAAFVE
CCCEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQIPVADRLALLEAEAKAARAEDDEDEPRAKSDRSERNDRAR
HCCCCCCEEEECCCCCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
RNRRRGGRRDGQNGEAQANGADKDLPAAEPVGPGFADYVAGTPAAALTQKGDIISEALDN
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHCCHHHHHHHCC
GDDDDVENEEDMVESVGSEDALEELPTHTRTHRRQYNIQEVIKRRQILLVQVVKEERGNK
CCCCCCCCHHHHHHHCCCHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GAALTTYLSLAGRYSVLMPNTARGGGISRKITNPQDRKRLKEIVKELEVPQGMSVILRTA
CCHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
GANRTKAEVKRDYEYLMRLWENVRTLTLQSTAPALVYEEGSLIKRSIRDLYNKDITEILV
CCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCHHHHHHHHHHHCCCHHHHEE
SGENGYREAKDFMRMLMPSHAKVVQPYRDHIPIFTRNGVEAQLDRMLVPQVTLKSGGYLI
CCCCCHHHHHHHHHHHCCCHHHHHCCHHHCCCEEECCCCHHHHHHHHCCCEEECCCCEEE
INQTEALVAIDVNSGRSTREHSIEDTALQTNLEAAEEVARQLRLRDLAGLIVVDFIDMEE
EECCCEEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRNNRAVEKKMKECLKDDRARIQVGRISHFGLLEMSRQRIRASVLESTMQVCPHCGGTGH
HHCCHHHHHHHHHHHHCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IRSDSSMALHIIRGIEDYLLRHSGFDIHVRTPAASALYVLNHKRQILADLEGRFGVEISI
CCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEE
DADESVGNQHFVIDKGAPSTRPVTPSAVQPMVYEDDIEDPEIPVEEDETEEEARTDVQAG
CCCCCCCCCEEEEECCCCCCCCCCHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHCC
EERSEENDRKRRRRRRRRGGRERDGRDLSAQEEAVSEEASEEDNETDQANEAVSSASMSE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCH
EDRRKKRRRGRRGGRKNRREDDNRARRIPDPEFVGYTPVLPVKAEESIGEVVETVETVEA
HHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
PVVEAAPEEKPEKPARKPRARKTAKTEEAAEAVITAEAEEKPEKPARKTRTRKKAVDEAP
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHCCC
ADEAATPAEEVVPEKPKRRTRKAAAKPVVEAEDVAEVKETVAAETQVETIAEPKSTEPVV
CCCCCCCHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
TSSESEDRKPKRSGWWQKKGFF
ECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]