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The map label for this gene is manX [H]

Identifier: 23100830

GI number: 23100830

Start: 3510017

End: 3510985

Strand: Reverse

Name: manX [H]

Synonym: OB3375

Alternate gene names: 23100830

Gene position: 3510985-3510017 (Counterclockwise)

Preceding gene: 23100831

Following gene: 23100829

Centisome position: 96.71

GC content: 42.83

Gene sequence:

>969_bases
ATGGTAGGAATTATCATTGCTACTCATGGTGAATTTGCCGAGGGTATCTTGCAATCTGGAGAGATGATCTTTGGAAAACA
AGAGAATGTAGAAGCTGTTACATTGATGCCGAGCGAAGGCCCTGATGATGTAAGGGCAAAAATGGAAAAAGCAATTGCCT
CTTTTGACAATCAAGACGAAGTTTTATTCTTAGTCGATCTTTGGGGTGGTACTCCTTTCAACCAAGCAAGCAGTTTAATG
GAAGCGCATCAAGATAAATGGGCGATCGTTGCTGGATTAAACTTAGCTATGTTGATTGAAGCTTTTGCGACACGCTTTTC
CAAGGAATCAGCGCATGAAATTGCTGTACATATTTTAGACACAGCAAAAGAAGCGGTGAAGGTAAAACCGGAAGAATTAG
AGCCAGCAGATGCTGCTGCAGATACTGCTCAGCAGGTGAATACAGGTGCTCCTGGTAAATTTGAATATGTACTAGCCCGT
ATTGACTCTCGCTTGCTGCATGGACAAGTAGCGACTGCGTGGACAAAGAACACACTGCCTACTCGTATCATCGTTGTATC
GGATGAAGTAGCGAAGGATGAATTGCGTAAGAAATTAATTCAGCAAGCTGCTCCATCAGGGGTGAAGGCACACGTTGTTC
CGATTAATAAAATGATTGAACTTGCCAAAGATGATAAACATTTTGGCGGTCAGCGAGCATTACTGCTTTTTGAAAATCCG
CATGATGTGCTTAAAGCAGTAGAAGGCGGTGTTCCATTAGAAACAATCAATGTTGGTTCGATGGCTCACTCGACTGGTAA
GGTGCAGCCGAATAAAGTATTGGCCTTTAGCCAAGCGGATCTCGACGCATTTAAAAAGCTAAAAGAACATGGATTGTCAT
TCGACGTACGTAAAGTACCGAACGATTCCAGAGGTAATATGGATGAGATTATGAGAAGGGCTCAGTCAGAGCTAGATAGA
CAGAGATAA

Upstream 100 bases:

>100_bases
CTAATCAAAAGTATAGCCGCCCTAGATAAGGGAATAAAATATACTTTAGGGATACCATGTTTATGTGAGTTTATATAATA
AAAATAGGGAGGTAAAAAAG

Downstream 100 bases:

>100_bases
TCCTAATCATCAGTAAAAAAAGGAGGATTAATGATTATGGAATTAACTATTATTCAAATAATATTAATTATTATTGTAGC
ATTTTTAGCTGGTGTAGAAG

Product: PTS system mannose-specific enzyme II AB component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MVGIIIATHGEFAEGILQSGEMIFGKQENVEAVTLMPSEGPDDVRAKMEKAIASFDNQDEVLFLVDLWGGTPFNQASSLM
EAHQDKWAIVAGLNLAMLIEAFATRFSKESAHEIAVHILDTAKEAVKVKPEELEPADAAADTAQQVNTGAPGKFEYVLAR
IDSRLLHGQVATAWTKNTLPTRIIVVSDEVAKDELRKKLIQQAAPSGVKAHVVPINKMIELAKDDKHFGGQRALLLFENP
HDVLKAVEGGVPLETINVGSMAHSTGKVQPNKVLAFSQADLDAFKKLKEHGLSFDVRKVPNDSRGNMDEIMRRAQSELDR
QR

Sequences:

>Translated_322_residues
MVGIIIATHGEFAEGILQSGEMIFGKQENVEAVTLMPSEGPDDVRAKMEKAIASFDNQDEVLFLVDLWGGTPFNQASSLM
EAHQDKWAIVAGLNLAMLIEAFATRFSKESAHEIAVHILDTAKEAVKVKPEELEPADAAADTAQQVNTGAPGKFEYVLAR
IDSRLLHGQVATAWTKNTLPTRIIVVSDEVAKDELRKKLIQQAAPSGVKAHVVPINKMIELAKDDKHFGGQRALLLFENP
HDVLKAVEGGVPLETINVGSMAHSTGKVQPNKVLAFSQADLDAFKKLKEHGLSFDVRKVPNDSRGNMDEIMRRAQSELDR
QR
>Mature_322_residues
MVGIIIATHGEFAEGILQSGEMIFGKQENVEAVTLMPSEGPDDVRAKMEKAIASFDNQDEVLFLVDLWGGTPFNQASSLM
EAHQDKWAIVAGLNLAMLIEAFATRFSKESAHEIAVHILDTAKEAVKVKPEELEPADAAADTAQQVNTGAPGKFEYVLAR
IDSRLLHGQVATAWTKNTLPTRIIVVSDEVAKDELRKKLIQQAAPSGVKAHVVPINKMIELAKDDKHFGGQRALLLFENP
HDVLKAVEGGVPLETINVGSMAHSTGKVQPNKVLAFSQADLDAFKKLKEHGLSFDVRKVPNDSRGNMDEIMRRAQSELDR
QR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=320, Percent_Identity=41.875, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082217, Length=148, Percent_Identity=35.8108108108108, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789527, Length=150, Percent_Identity=34.6666666666667, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR004720 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35260; Mature: 35260

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGIIIATHGEFAEGILQSGEMIFGKQENVEAVTLMPSEGPDDVRAKMEKAIASFDNQDE
CEEEEEECCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
VLFLVDLWGGTPFNQASSLMEAHQDKWAIVAGLNLAMLIEAFATRFSKESAHEIAVHILD
EEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TAKEAVKVKPEELEPADAAADTAQQVNTGAPGKFEYVLARIDSRLLHGQVATAWTKNTLP
HHHHHHCCCCHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHEECCCCC
TRIIVVSDEVAKDELRKKLIQQAAPSGVKAHVVPINKMIELAKDDKHFGGQRALLLFENP
EEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCEEEEEEECH
HDVLKAVEGGVPLETINVGSMAHSTGKVQPNKVLAFSQADLDAFKKLKEHGLSFDVRKVP
HHHHHHHCCCCCEEEEECCCHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECC
NDSRGNMDEIMRRAQSELDRQR
CCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVGIIIATHGEFAEGILQSGEMIFGKQENVEAVTLMPSEGPDDVRAKMEKAIASFDNQDE
CEEEEEECCCHHHHHHHHCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCC
VLFLVDLWGGTPFNQASSLMEAHQDKWAIVAGLNLAMLIEAFATRFSKESAHEIAVHILD
EEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHH
TAKEAVKVKPEELEPADAAADTAQQVNTGAPGKFEYVLARIDSRLLHGQVATAWTKNTLP
HHHHHHCCCCHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHEECCCCC
TRIIVVSDEVAKDELRKKLIQQAAPSGVKAHVVPINKMIELAKDDKHFGGQRALLLFENP
EEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCEEEEEEECH
HDVLKAVEGGVPLETINVGSMAHSTGKVQPNKVLAFSQADLDAFKKLKEHGLSFDVRKVP
HHHHHHHCCCCCEEEEECCCHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECC
NDSRGNMDEIMRRAQSELDRQR
CCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA