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The map label for this gene is manZ [H]

Identifier: 23100828

GI number: 23100828

Start: 3508243

End: 3509157

Strand: Reverse

Name: manZ [H]

Synonym: OB3373

Alternate gene names: 23100828

Gene position: 3509157-3508243 (Counterclockwise)

Preceding gene: 23100829

Following gene: 23100827

Centisome position: 96.66

GC content: 43.72

Gene sequence:

>915_bases
ATGGCTCGAGAAGAATTGAAATTAACAAAAAGAGATCGGATTGCGATTTGGTGGCGATCCACGTTCATTCAGGGTTCTTG
GAACTATGAACGTATGCAAAACGGTGGTTGGGCATTTTCCATGATTCCTGCCATCAAGAGATTGTATAAAACAAAAGAAG
ATCGTGCTGCAGCACTACAACGTCATTTGGAATTCTTTAATACTCATCCATATGTAGCTTCTCCGATTATTGGTGTAACG
TTAGCGCTTGAAGAAGAACGGGCAAACGGCGCGCCAGTGGATAACAAGGCAATTCAAGGTGTAAAAGTTGGTATGATGGG
ACCTCTAGCAGGTATTGGGGATCCAATGTTCTGGTTCACAGTAAAACCAATCCTTGGTGCATTAGCGGCTTCGCTTGCGC
TTAGTGGTAACATACTCGGACCGATTCTTTACTTTGTATTATGGAATCTTATTCGTATGGGCTTCAAATGGTATTCTCAG
GAACTTGGTTACAAAGCTGGCTCGAAAATTACCGAGGACTTATCGGGCGGATTACTACAGGATATAACCAAAGGTGCTTC
GATACTCGGTATGTTCATCCTCGGTGCTTTAGTAAATAGGTGGGTAGCTGTCTCATTTGCTCCGACCGTATCCGAGGTTC
AATTGGACGAAGGTGCCTATATCGATTGGGAAAGCTTACCTTCAGGAGCGGAAGGAATTCGAACCGCGCTGATTCAGCAA
GAACAGGGTCGATCACTGACCCAGACCGATGTGACGACCTTACAGGACAACCTGGATAGCTTAATTCCTGGTTTAGCTGG
TTTGCTACTTACACTTCTATGTATGTGGCTGCTAAGGAAGAAAGTTTCTCCAATCATTATGATTCTTGGATTGTTCGTAG
TAGGTATAGTATTCCACGTTATCGGATTAATGTAA

Upstream 100 bases:

>100_bases
GCACTTACAAAACAAGGCGGTTCTGGTAATGGTGGAAACGGAAACACTGGTGATCCGCTAGGTGAAATCATAGATAACTA
CTAAAAAGGAGGGACTAAAA

Downstream 100 bases:

>100_bases
GAAATAATTGAGATTGGCTCAAATAGATAAGTACCTCCTTGTATGATAGTCCTATCAAGCTAAGGAGGTATTTTTCTTGT
GTTTCAGGAACGCAGGACAT

Product: PTS system mannose-specific enzyme II D component

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAREELKLTKRDRIAIWWRSTFIQGSWNYERMQNGGWAFSMIPAIKRLYKTKEDRAAALQRHLEFFNTHPYVASPIIGVT
LALEEERANGAPVDNKAIQGVKVGMMGPLAGIGDPMFWFTVKPILGALAASLALSGNILGPILYFVLWNLIRMGFKWYSQ
ELGYKAGSKITEDLSGGLLQDITKGASILGMFILGALVNRWVAVSFAPTVSEVQLDEGAYIDWESLPSGAEGIRTALIQQ
EQGRSLTQTDVTTLQDNLDSLIPGLAGLLLTLLCMWLLRKKVSPIIMILGLFVVGIVFHVIGLM

Sequences:

>Translated_304_residues
MAREELKLTKRDRIAIWWRSTFIQGSWNYERMQNGGWAFSMIPAIKRLYKTKEDRAAALQRHLEFFNTHPYVASPIIGVT
LALEEERANGAPVDNKAIQGVKVGMMGPLAGIGDPMFWFTVKPILGALAASLALSGNILGPILYFVLWNLIRMGFKWYSQ
ELGYKAGSKITEDLSGGLLQDITKGASILGMFILGALVNRWVAVSFAPTVSEVQLDEGAYIDWESLPSGAEGIRTALIQQ
EQGRSLTQTDVTTLQDNLDSLIPGLAGLLLTLLCMWLLRKKVSPIIMILGLFVVGIVFHVIGLM
>Mature_303_residues
AREELKLTKRDRIAIWWRSTFIQGSWNYERMQNGGWAFSMIPAIKRLYKTKEDRAAALQRHLEFFNTHPYVASPIIGVTL
ALEEERANGAPVDNKAIQGVKVGMMGPLAGIGDPMFWFTVKPILGALAASLALSGNILGPILYFVLWNLIRMGFKWYSQE
LGYKAGSKITEDLSGGLLQDITKGASILGMFILGALVNRWVAVSFAPTVSEVQLDEGAYIDWESLPSGAEGIRTALIQQE
QGRSLTQTDVTTLQDNLDSLIPGLAGLLLTLLCMWLLRKKVSPIIMILGLFVVGIVFHVIGLM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=301, Percent_Identity=50.1661129568106, Blast_Score=308, Evalue=3e-85,
Organism=Escherichia coli, GI1789529, Length=303, Percent_Identity=32.6732673267327, Blast_Score=164, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: NA

Molecular weight: Translated: 33583; Mature: 33452

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAREELKLTKRDRIAIWWRSTFIQGSWNYERMQNGGWAFSMIPAIKRLYKTKEDRAAALQ
CCCHHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
RHLEFFNTHPYVASPIIGVTLALEEERANGAPVDNKAIQGVKVGMMGPLAGIGDPMFWFT
HHHHHHCCCCCHHCCHHHEEEEEEHHCCCCCCCCCHHHCCEEECCCCCCCCCCCCHHHHH
VKPILGALAASLALSGNILGPILYFVLWNLIRMGFKWYSQELGYKAGSKITEDLSGGLLQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHH
DITKGASILGMFILGALVNRWVAVSFAPTVSEVQLDEGAYIDWESLPSGAEGIRTALIQQ
HHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHEEECCCCEECHHHCCCCHHHHHHHHHHH
EQGRSLTQTDVTTLQDNLDSLIPGLAGLLLTLLCMWLLRKKVSPIIMILGLFVVGIVFHV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLM
HHCC
>Mature Secondary Structure 
AREELKLTKRDRIAIWWRSTFIQGSWNYERMQNGGWAFSMIPAIKRLYKTKEDRAAALQ
CCHHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
RHLEFFNTHPYVASPIIGVTLALEEERANGAPVDNKAIQGVKVGMMGPLAGIGDPMFWFT
HHHHHHCCCCCHHCCHHHEEEEEEHHCCCCCCCCCHHHCCEEECCCCCCCCCCCCHHHHH
VKPILGALAASLALSGNILGPILYFVLWNLIRMGFKWYSQELGYKAGSKITEDLSGGLLQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHH
DITKGASILGMFILGALVNRWVAVSFAPTVSEVQLDEGAYIDWESLPSGAEGIRTALIQQ
HHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHEEECCCCEECHHHCCCCHHHHHHHHHHH
EQGRSLTQTDVTTLQDNLDSLIPGLAGLLLTLLCMWLLRKKVSPIIMILGLFVVGIVFHV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLM
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]