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The map label for this gene is ydjJ [H]

Identifier: 23100713

GI number: 23100713

Start: 3394250

End: 3395278

Strand: Reverse

Name: ydjJ [H]

Synonym: OB3258

Alternate gene names: 23100713

Gene position: 3395278-3394250 (Counterclockwise)

Preceding gene: 23100714

Following gene: 23100712

Centisome position: 93.52

GC content: 39.75

Gene sequence:

>1029_bases
ATGAAAGCGGTATTTGTAGAAGATGCTGAGAAAATTATTGTGAAAGATATAGAGGTCCCCGAGTTAAAACCGAATGAGGT
GCAAATCAAAGTAAATGTCGCTGGGATTTGCGGTTCCGATATTCATACGTATAAAGGCTTACATCCATTTCGTAAACCCC
CGGTCGTTATTGGACATGAAGTAGCTGGAGAAGTTGTAGAAGTTGGATCATCCGTTACTAAATTCAAGGTTGGTGATCGA
GTAACGGTGGAGCCGCAAATCGGTACAGGTGAGAGTGAAGGAGTAATGACGGGAAATGTAAATTACTCTGATACAAGACT
AGCTCCAGGAATGGGAGACTGGTTAGGGACAATGGCTGAATATTTCGCATCGCCTGAAGAACAAGTAATTAAATTACCAG
ATTCCGTTAGTTATGATCAAGGTGTATTAGTCGAACCTCTTGCTGTAGGTGTACATGCAGCGTTTAAAGGCGATGTTCAA
CCAACCGATCGAGTAGCAATTTTGGGAGCCGGACCAATTGGCCTTCTTACACTTATCGCTGTGAAAGCTAAAGGCGTAAA
AGATATTGTTGTTACGGATGTGCTCGACTATTCACTACAAGTGGCAAAAGAAATGGGTGCTTCAGAAGTTATTAATATTA
AGGATAATTCAAACTGGATTCATGATGCGAAGCAGAGCTTAGATAAAGGATCTTTTGATAAAGTATTTATCACTGCGGGT
GTTCCTGGTATTGTAAATGACTCTCTTAATCTACTAAGAAAAGGTGGACGAGTGGTTACCGTAGCCATGTTCCATGGACA
ACAGTCTGTTGATATCGAACAGTTACAGCAAAATGAAAAAGAAATTATTGGGTGCATGACATATAACCGTCCGGATACAG
AAGAAGCCCTAGAAATAATCGCAAGCGGGAAACTTGATGTAGAGAAGGTTATTTCCCATAAATTGCATTATGAAGAAGCA
GCAAAGGGTTTTACAATTGTAGATAAAAAGAAAGATAAATCCGTTAAAGTACTTGTTTCATTTGAATAG

Upstream 100 bases:

>100_bases
GGAGATATAAAGTGCGTAGAGTTAATGAAAAAGCATCTATTGGACTCCAAGATGATGTACTTTCAATAACTCCTGCTGAT
ATAAGAGGGAGAGATAGTTT

Downstream 100 bases:

>100_bases
GGGGATAAAAATGAAATCAAAAATAATCGTATTACTTTTATCGGCTGTAGCTTTACTATTAGCTGCGTGTAGTCAAGGAA
CATCGAGTGCTGAGGAACCA

Product: sorbitol dehydrogenase

Products: L-sorbose; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKAVFVEDAEKIIVKDIEVPELKPNEVQIKVNVAGICGSDIHTYKGLHPFRKPPVVIGHEVAGEVVEVGSSVTKFKVGDR
VTVEPQIGTGESEGVMTGNVNYSDTRLAPGMGDWLGTMAEYFASPEEQVIKLPDSVSYDQGVLVEPLAVGVHAAFKGDVQ
PTDRVAILGAGPIGLLTLIAVKAKGVKDIVVTDVLDYSLQVAKEMGASEVINIKDNSNWIHDAKQSLDKGSFDKVFITAG
VPGIVNDSLNLLRKGGRVVTVAMFHGQQSVDIEQLQQNEKEIIGCMTYNRPDTEEALEIIASGKLDVEKVISHKLHYEEA
AKGFTIVDKKKDKSVKVLVSFE

Sequences:

>Translated_342_residues
MKAVFVEDAEKIIVKDIEVPELKPNEVQIKVNVAGICGSDIHTYKGLHPFRKPPVVIGHEVAGEVVEVGSSVTKFKVGDR
VTVEPQIGTGESEGVMTGNVNYSDTRLAPGMGDWLGTMAEYFASPEEQVIKLPDSVSYDQGVLVEPLAVGVHAAFKGDVQ
PTDRVAILGAGPIGLLTLIAVKAKGVKDIVVTDVLDYSLQVAKEMGASEVINIKDNSNWIHDAKQSLDKGSFDKVFITAG
VPGIVNDSLNLLRKGGRVVTVAMFHGQQSVDIEQLQQNEKEIIGCMTYNRPDTEEALEIIASGKLDVEKVISHKLHYEEA
AKGFTIVDKKKDKSVKVLVSFE
>Mature_342_residues
MKAVFVEDAEKIIVKDIEVPELKPNEVQIKVNVAGICGSDIHTYKGLHPFRKPPVVIGHEVAGEVVEVGSSVTKFKVGDR
VTVEPQIGTGESEGVMTGNVNYSDTRLAPGMGDWLGTMAEYFASPEEQVIKLPDSVSYDQGVLVEPLAVGVHAAFKGDVQ
PTDRVAILGAGPIGLLTLIAVKAKGVKDIVVTDVLDYSLQVAKEMGASEVINIKDNSNWIHDAKQSLDKGSFDKVFITAG
VPGIVNDSLNLLRKGGRVVTVAMFHGQQSVDIEQLQQNEKEIIGCMTYNRPDTEEALEIIASGKLDVEKVISHKLHYEEA
AKGFTIVDKKKDKSVKVLVSFE

Specific function: Unknown

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=320, Percent_Identity=32.8125, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=26.2872628726287, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4501929, Length=368, Percent_Identity=26.0869565217391, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI4501933, Length=368, Percent_Identity=26.3586956521739, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=25.7372654155496, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI156523966, Length=332, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4501939, Length=343, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788073, Length=331, Percent_Identity=38.368580060423, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790045, Length=347, Percent_Identity=30.835734870317, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787863, Length=344, Percent_Identity=30.5232558139535, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI226510992, Length=326, Percent_Identity=26.9938650306748, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1788075, Length=348, Percent_Identity=26.7241379310345, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788407, Length=337, Percent_Identity=29.080118694362, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87082125, Length=358, Percent_Identity=26.8156424581006, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790718, Length=326, Percent_Identity=28.5276073619632, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1786825, Length=396, Percent_Identity=26.010101010101, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081918, Length=309, Percent_Identity=30.0970873786408, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786552, Length=368, Percent_Identity=25.2717391304348, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI48994910, Length=348, Percent_Identity=26.7241379310345, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786518, Length=328, Percent_Identity=26.8292682926829, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082401, Length=297, Percent_Identity=26.9360269360269, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17562876, Length=325, Percent_Identity=34.7692307692308, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17562878, Length=314, Percent_Identity=33.1210191082803, Blast_Score=147, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71988145, Length=299, Percent_Identity=28.0936454849498, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17562584, Length=300, Percent_Identity=27.3333333333333, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319955, Length=331, Percent_Identity=36.2537764350453, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322619, Length=331, Percent_Identity=36.2537764350453, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323099, Length=345, Percent_Identity=30.7246376811594, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319257, Length=357, Percent_Identity=29.1316526610644, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319258, Length=377, Percent_Identity=28.1167108753316, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319621, Length=305, Percent_Identity=30.1639344262295, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323729, Length=307, Percent_Identity=29.6416938110749, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320033, Length=354, Percent_Identity=24.8587570621469, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=306, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=306, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137530, Length=334, Percent_Identity=34.1317365269461, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17737897, Length=342, Percent_Identity=31.2865497076023, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=25.2717391304348, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI221457811, Length=192, Percent_Identity=31.25, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550770, Length=192, Percent_Identity=31.25, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45551930, Length=192, Percent_Identity=31.25, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.14

Molecular weight: Translated: 37003; Mature: 37003

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVFVEDAEKIIVKDIEVPELKPNEVQIKVNVAGICGSDIHTYKGLHPFRKPPVVIGHE
CCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCHHHHCCCCCCCCCCEEECHH
VAGEVVEVGSSVTKFKVGDRVTVEPQIGTGESEGVMTGNVNYSDTRLAPGMGDWLGTMAE
HHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
YFASPEEQVIKLPDSVSYDQGVLVEPLAVGVHAAFKGDVQPTDRVAILGAGPIGLLTLIA
HHCCCCHHEEECCCCCCCCCCCEECHHHHEEEEEECCCCCCCCCEEEEECCHHHHHHHHH
VKAKGVKDIVVTDVLDYSLQVAKEMGASEVINIKDNSNWIHDAKQSLDKGSFDKVFITAG
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEC
VPGIVNDSLNLLRKGGRVVTVAMFHGQQSVDIEQLQQNEKEIIGCMTYNRPDTEEALEII
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCHHHEEEEEECCCCCHHHHHHHH
ASGKLDVEKVISHKLHYEEAAKGFTIVDKKKDKSVKVLVSFE
HCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEC
>Mature Secondary Structure
MKAVFVEDAEKIIVKDIEVPELKPNEVQIKVNVAGICGSDIHTYKGLHPFRKPPVVIGHE
CCEEEECCCCEEEEECCCCCCCCCCEEEEEEEEEEEECCCHHHHCCCCCCCCCCEEECHH
VAGEVVEVGSSVTKFKVGDRVTVEPQIGTGESEGVMTGNVNYSDTRLAPGMGDWLGTMAE
HHHHHHHHCCCEEEEECCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHH
YFASPEEQVIKLPDSVSYDQGVLVEPLAVGVHAAFKGDVQPTDRVAILGAGPIGLLTLIA
HHCCCCHHEEECCCCCCCCCCCEECHHHHEEEEEECCCCCCCCCEEEEECCHHHHHHHHH
VKAKGVKDIVVTDVLDYSLQVAKEMGASEVINIKDNSNWIHDAKQSLDKGSFDKVFITAG
HHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEC
VPGIVNDSLNLLRKGGRVVTVAMFHGQQSVDIEQLQQNEKEIIGCMTYNRPDTEEALEII
CCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCHHHEEEEEECCCCCHHHHHHHH
ASGKLDVEKVISHKLHYEEAAKGFTIVDKKKDKSVKVLVSFE
HCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-iditol; NAD+

Specific reaction: L-iditol + NAD+ = L-sorbose + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]