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The map label for this gene is ilvB [H]

Identifier: 23100692

GI number: 23100692

Start: 3373657

End: 3375417

Strand: Reverse

Name: ilvB [H]

Synonym: OB3237

Alternate gene names: 23100692

Gene position: 3375417-3373657 (Counterclockwise)

Preceding gene: 23100693

Following gene: 23100688

Centisome position: 92.97

GC content: 39.07

Gene sequence:

>1761_bases
ATGAAAAGACTAGTTTCTAATTTGTTAGTTGAATATTTGGAAGATAGAGGTGTGGAACATATATTTGGATTGTGTGGTCA
CACCAACATAGCATTCCTATCGCAGCTTGAGAAAAGTTCGATTAACTTTGTCAATGTACGTCACGAACAAATAGCAGCCC
ATATGGCAGATGGATATGCGAGAGCAAAAAAAGAGACCTCAGTAGTTTTAAGTCATTTAGGCCCAGGATTAACGAATGCC
GCAACCGGGGTTGCAAATGCAGCTTTAGATAACATACCAATGGTTGTTATTGCAGGAGATGTACCTAGCCATTATTATGG
AAAACATCCTCATCAAGAAGTTAACTATCATGCAGATGGATCCCAATATGAAATCTATCGTCCATTCGTGAAACGTGCAT
GGCGTGTAGATCGTCCAGATTTATTTCCAGAGATTATCGAAAAAGCATTTCAACTTGCTGAAAGTGGTAAACCTGGTCCA
GTATTAGTTTCCGTACCAATGGATATGTTCTCAAAAGAAATAGATGACACACTGTTTGAGAAAACAAAGCATCATACGAG
ATCGATTGCCAAACCATCTATTAATGAGGAAAAAGCGAAAGAAATTATTCTATCACTCTTAGAAGCTAAATCTCCTGTAA
TTTATGCAGGCGGTGGGGTTATTTTAGCGGATGCAGCAGAAGAATTACTTGAATTTGTGAATCATTTTGATATACCAGTA
GCACATACCTTAATGGGTAAAGGAGTTATCCCAGATGATAATGAATTAGTATTAGGGATGACAGGTTTTTGGGGTACGAA
ATTTATTAATGAAAAAACAAGAACTGCTGATCAAATCTTTGCTTTAGGAACGCAGTTTGCCGAAGCGGATAGCAGCTCTT
GGGAACCGGAATACACATTTAATATTCCTGATACGAAGCTTATACAAATAGATATAGATCCGAATGAAATCGGGAGAAAT
TATCCTGTTGAAATAGGGGTAGTAGCTGATTTAAAAGAGGCTCTTACCGTATTAAATCGTGTAGCCAAAGAGATCGCTCC
TGATGGCGTATCGAATAGTAAAATCAAGGAAGAAATTAAAAACTACCGTAAAGAATTTACACAGAGTAACCTAGAAGCAA
GCGAATCGAATAGTTATCCACTATCTCCCCAAAGGATTTTAGCGGATGTAAGGTCCGTTATCCCAAGGGATGCATATATT
ACAACTGATGTTGGTTGGAATAAAAACGGAGTCGGACAGCAATTTCCAATTTATGAACCAGGAAGTATTCTCACACCTGG
TGGCTATGCAACAATGGGATTTGGCGCACCTGCAGCAATGGGGGCTAAAATTGCCAAGAAAGATAAAGTAGTAATTTCAT
TAGTAGGAGACGGAGGATTTGGACAAAACCCAGCACTTTTAGCCACTGCCGTGGAAGAGAATATTCCGGTAATATGGATC
GTAATGAATAACTCATCCTATGGCACGATTGCTGGGTTAGAAAAAGCGCACTTTGACACAACCTATGGAACAGTTTTCAA
GCGTGGGGAAGACACTTATTCTCCAAATTATGCAGAAATTGCAAAAGGCTATGGCGTTGAAGCGGTAAAAATTCAGTCTG
CGGATGAATTTAAACCCGCACTGGAACAAGCGATTGCATCCAATAGACCATTTGTATTAGATGTGCCGATGGTAAATAAT
CCAGTCCCTACAGATGGGCATTGGAATATCATGGATATCTATTCTCCAGGCAAAAAAGTTCATCATGAAAGTGTTCAATA
A

Upstream 100 bases:

>100_bases
TTCAAAAATTCTTTCTGTAAACGAATATGAAAAGGCATACACGTATGCGGTATCAGGAGATTATGGGAAAATTATTTTGA
AATTCTAGGAGGTAATGTAC

Downstream 100 bases:

>100_bases
AACAGATATACAAGGGGCTAGGACATAATTAGCCTTAAGAAGAAAAAAGATTCCAGTTATCAATTAGATAGCTGGAATCT
TTTTTCATGGTCATCTATAC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKRLVSNLLVEYLEDRGVEHIFGLCGHTNIAFLSQLEKSSINFVNVRHEQIAAHMADGYARAKKETSVVLSHLGPGLTNA
ATGVANAALDNIPMVVIAGDVPSHYYGKHPHQEVNYHADGSQYEIYRPFVKRAWRVDRPDLFPEIIEKAFQLAESGKPGP
VLVSVPMDMFSKEIDDTLFEKTKHHTRSIAKPSINEEKAKEIILSLLEAKSPVIYAGGGVILADAAEELLEFVNHFDIPV
AHTLMGKGVIPDDNELVLGMTGFWGTKFINEKTRTADQIFALGTQFAEADSSSWEPEYTFNIPDTKLIQIDIDPNEIGRN
YPVEIGVVADLKEALTVLNRVAKEIAPDGVSNSKIKEEIKNYRKEFTQSNLEASESNSYPLSPQRILADVRSVIPRDAYI
TTDVGWNKNGVGQQFPIYEPGSILTPGGYATMGFGAPAAMGAKIAKKDKVVISLVGDGGFGQNPALLATAVEENIPVIWI
VMNNSSYGTIAGLEKAHFDTTYGTVFKRGEDTYSPNYAEIAKGYGVEAVKIQSADEFKPALEQAIASNRPFVLDVPMVNN
PVPTDGHWNIMDIYSPGKKVHHESVQ

Sequences:

>Translated_586_residues
MKRLVSNLLVEYLEDRGVEHIFGLCGHTNIAFLSQLEKSSINFVNVRHEQIAAHMADGYARAKKETSVVLSHLGPGLTNA
ATGVANAALDNIPMVVIAGDVPSHYYGKHPHQEVNYHADGSQYEIYRPFVKRAWRVDRPDLFPEIIEKAFQLAESGKPGP
VLVSVPMDMFSKEIDDTLFEKTKHHTRSIAKPSINEEKAKEIILSLLEAKSPVIYAGGGVILADAAEELLEFVNHFDIPV
AHTLMGKGVIPDDNELVLGMTGFWGTKFINEKTRTADQIFALGTQFAEADSSSWEPEYTFNIPDTKLIQIDIDPNEIGRN
YPVEIGVVADLKEALTVLNRVAKEIAPDGVSNSKIKEEIKNYRKEFTQSNLEASESNSYPLSPQRILADVRSVIPRDAYI
TTDVGWNKNGVGQQFPIYEPGSILTPGGYATMGFGAPAAMGAKIAKKDKVVISLVGDGGFGQNPALLATAVEENIPVIWI
VMNNSSYGTIAGLEKAHFDTTYGTVFKRGEDTYSPNYAEIAKGYGVEAVKIQSADEFKPALEQAIASNRPFVLDVPMVNN
PVPTDGHWNIMDIYSPGKKVHHESVQ
>Mature_586_residues
MKRLVSNLLVEYLEDRGVEHIFGLCGHTNIAFLSQLEKSSINFVNVRHEQIAAHMADGYARAKKETSVVLSHLGPGLTNA
ATGVANAALDNIPMVVIAGDVPSHYYGKHPHQEVNYHADGSQYEIYRPFVKRAWRVDRPDLFPEIIEKAFQLAESGKPGP
VLVSVPMDMFSKEIDDTLFEKTKHHTRSIAKPSINEEKAKEIILSLLEAKSPVIYAGGGVILADAAEELLEFVNHFDIPV
AHTLMGKGVIPDDNELVLGMTGFWGTKFINEKTRTADQIFALGTQFAEADSSSWEPEYTFNIPDTKLIQIDIDPNEIGRN
YPVEIGVVADLKEALTVLNRVAKEIAPDGVSNSKIKEEIKNYRKEFTQSNLEASESNSYPLSPQRILADVRSVIPRDAYI
TTDVGWNKNGVGQQFPIYEPGSILTPGGYATMGFGAPAAMGAKIAKKDKVVISLVGDGGFGQNPALLATAVEENIPVIWI
VMNNSSYGTIAGLEKAHFDTTYGTVFKRGEDTYSPNYAEIAKGYGVEAVKIQSADEFKPALEQAIASNRPFVLDVPMVNN
PVPTDGHWNIMDIYSPGKKVHHESVQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=593, Percent_Identity=26.6441821247892, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21361361, Length=525, Percent_Identity=26.0952380952381, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=32.3214285714286, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1786717, Length=543, Percent_Identity=31.8600368324125, Blast_Score=260, Evalue=2e-70,
Organism=Escherichia coli, GI1790104, Length=552, Percent_Identity=30.9782608695652, Blast_Score=246, Evalue=4e-66,
Organism=Escherichia coli, GI1787096, Length=556, Percent_Identity=26.6187050359712, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=24.3291592128801, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=592, Percent_Identity=26.0135135135135, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=578, Percent_Identity=26.643598615917, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531301, Length=578, Percent_Identity=26.643598615917, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=28.7215411558669, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6320816, Length=554, Percent_Identity=24.7292418772563, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320588, Length=585, Percent_Identity=22.7350427350427, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25.1748251748252, Blast_Score=159, Evalue=5e-39,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64338; Mature: 64338

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLVSNLLVEYLEDRGVEHIFGLCGHTNIAFLSQLEKSSINFVNVRHEQIAAHMADGYA
CHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
RAKKETSVVLSHLGPGLTNAATGVANAALDNIPMVVIAGDVPSHYYGKHPHQEVNYHADG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
SQYEIYRPFVKRAWRVDRPDLFPEIIEKAFQLAESGKPGPVLVSVPMDMFSKEIDDTLFE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
KTKHHTRSIAKPSINEEKAKEIILSLLEAKSPVIYAGGGVILADAAEELLEFVNHFDIPV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCCH
AHTLMGKGVIPDDNELVLGMTGFWGTKFINEKTRTADQIFALGTQFAEADSSSWEPEYTF
HHHHHCCCCCCCCCCEEEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
NIPDTKLIQIDIDPNEIGRNYPVEIGVVADLKEALTVLNRVAKEIAPDGVSNSKIKEEIK
ECCCCEEEEEECCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
NYRKEFTQSNLEASESNSYPLSPQRILADVRSVIPRDAYITTDVGWNKNGVGQQFPIYEP
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
GSILTPGGYATMGFGAPAAMGAKIAKKDKVVISLVGDGGFGQNPALLATAVEENIPVIWI
CCEECCCCCEECCCCCCHHHCCHHCCCCCEEEEEEECCCCCCCCCEEEEEHHCCCCEEEE
VMNNSSYGTIAGLEKAHFDTTYGTVFKRGEDTYSPNYAEIAKGYGVEAVKIQSADEFKPA
EECCCCCCEEECCHHHHCCCHHHHHEECCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHH
LEQAIASNRPFVLDVPMVNNPVPTDGHWNIMDIYSPGKKVHHESVQ
HHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCHHHCCCCC
>Mature Secondary Structure
MKRLVSNLLVEYLEDRGVEHIFGLCGHTNIAFLSQLEKSSINFVNVRHEQIAAHMADGYA
CHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHH
RAKKETSVVLSHLGPGLTNAATGVANAALDNIPMVVIAGDVPSHYYGKHPHQEVNYHADG
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCC
SQYEIYRPFVKRAWRVDRPDLFPEIIEKAFQLAESGKPGPVLVSVPMDMFSKEIDDTLFE
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
KTKHHTRSIAKPSINEEKAKEIILSLLEAKSPVIYAGGGVILADAAEELLEFVNHFDIPV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHCCCCH
AHTLMGKGVIPDDNELVLGMTGFWGTKFINEKTRTADQIFALGTQFAEADSSSWEPEYTF
HHHHHCCCCCCCCCCEEEEECCCCCHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
NIPDTKLIQIDIDPNEIGRNYPVEIGVVADLKEALTVLNRVAKEIAPDGVSNSKIKEEIK
ECCCCEEEEEECCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
NYRKEFTQSNLEASESNSYPLSPQRILADVRSVIPRDAYITTDVGWNKNGVGQQFPIYEP
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCC
GSILTPGGYATMGFGAPAAMGAKIAKKDKVVISLVGDGGFGQNPALLATAVEENIPVIWI
CCEECCCCCEECCCCCCHHHCCHHCCCCCEEEEEEECCCCCCCCCEEEEEHHCCCCEEEE
VMNNSSYGTIAGLEKAHFDTTYGTVFKRGEDTYSPNYAEIAKGYGVEAVKIQSADEFKPA
EECCCCCCEEECCHHHHCCCHHHHHEECCCCCCCCCHHHHHHCCCCEEEEECCCHHHHHH
LEQAIASNRPFVLDVPMVNNPVPTDGHWNIMDIYSPGKKVHHESVQ
HHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]