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The map label for this gene is yagI [H]

Identifier: 23100688

GI number: 23100688

Start: 3368996

End: 3369763

Strand: Reverse

Name: yagI [H]

Synonym: OB3233

Alternate gene names: 23100688

Gene position: 3369763-3368996 (Counterclockwise)

Preceding gene: 23100692

Following gene: 23100687

Centisome position: 92.82

GC content: 33.85

Gene sequence:

>768_bases
ATGAATGATTCTCCAAAAGGAATTAGAACCTTACAAAGATCGATTGATATATTAAATTGTTTTATCGAGAAGAATTCTGA
ATTAACATTAACTGAAATATCCCTATACACAGGATTAGCCAAGTCTACTACTACAAGATTATTATCCACTCTAGAGATGA
ATAATTTTGTAGAAAAGGATGAAGTAAATGCTAAATACAGGCTAGGTAAGCAAATTTATTTTCTCGGATTTGTAGCGGGA
CAAACTTTTGAACTGAATTCATTAGCTAAATCAACTATGGAAAGATTGAGGGAACAGACAAAAGAAACGGTAAACCTTTA
TATATTGGATGGAAAACATCGAGTATGTGTCCAGCAATTTGAGAGTTTGCAATCTGTTAAACATATGATTAGTGTAGGTC
AAAAACTACCGTTAACAGTAGGAGCAAGCGGGAAAGTATTTCTAGCCTATCAATCAAAAGAATTTATTGAAGATGCGATG
GATACACAGCCATTAAAAAAGAGTAAAGTAGATTTGAAGAATGAACTGGATTTAATTATAAAGGAAAAATATGCAGTTAG
TATTGAGGAAAGAGAATCTGGTACATCAGCTGCAGCAGCACCTATTTTTAATTTTCAAAATGAAGTTGTTGCCGTTTTAT
CTGTGTCTGGACCTGCAAGCAGATTAAAACCAAGGGAATGGCCCGAGTTGAGAAAGATGGTGATGGAGGCTTCCGTTGAG
ATCTCTAAAAATCTAGGATATATAAGTAATCGAGCTCATGAAAATTAA

Upstream 100 bases:

>100_bases
AGAGGTTATTTATTAGAGAATTGAAATAGCAATGTTAATAATCTATTATAAAATTACGATGGGATCTATATAAGAATGAG
GGAGGGGAGAAAAGTTGGAA

Downstream 100 bases:

>100_bases
TATAAGTACTTATGATGGTTGGTATCCTATAATGTTGAAAAATCTCTTATTCTTTCCATAAGGGATTTTGTTATTTTATA
GGTAACTACAGTCAGTTATT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNDSPKGIRTLQRSIDILNCFIEKNSELTLTEISLYTGLAKSTTTRLLSTLEMNNFVEKDEVNAKYRLGKQIYFLGFVAG
QTFELNSLAKSTMERLREQTKETVNLYILDGKHRVCVQQFESLQSVKHMISVGQKLPLTVGASGKVFLAYQSKEFIEDAM
DTQPLKKSKVDLKNELDLIIKEKYAVSIEERESGTSAAAAPIFNFQNEVVAVLSVSGPASRLKPREWPELRKMVMEASVE
ISKNLGYISNRAHEN

Sequences:

>Translated_255_residues
MNDSPKGIRTLQRSIDILNCFIEKNSELTLTEISLYTGLAKSTTTRLLSTLEMNNFVEKDEVNAKYRLGKQIYFLGFVAG
QTFELNSLAKSTMERLREQTKETVNLYILDGKHRVCVQQFESLQSVKHMISVGQKLPLTVGASGKVFLAYQSKEFIEDAM
DTQPLKKSKVDLKNELDLIIKEKYAVSIEERESGTSAAAAPIFNFQNEVVAVLSVSGPASRLKPREWPELRKMVMEASVE
ISKNLGYISNRAHEN
>Mature_255_residues
MNDSPKGIRTLQRSIDILNCFIEKNSELTLTEISLYTGLAKSTTTRLLSTLEMNNFVEKDEVNAKYRLGKQIYFLGFVAG
QTFELNSLAKSTMERLREQTKETVNLYILDGKHRVCVQQFESLQSVKHMISVGQKLPLTVGASGKVFLAYQSKEFIEDAM
DTQPLKKSKVDLKNELDLIIKEKYAVSIEERESGTSAAAAPIFNFQNEVVAVLSVSGPASRLKPREWPELRKMVMEASVE
ISKNLGYISNRAHEN

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=31.0483870967742, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082359, Length=248, Percent_Identity=29.0322580645161, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788131, Length=254, Percent_Identity=27.9527559055118, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786715, Length=255, Percent_Identity=29.0196078431373, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1789999, Length=255, Percent_Identity=26.2745098039216, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788581, Length=253, Percent_Identity=26.8774703557312, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28746; Mature: 28746

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSPKGIRTLQRSIDILNCFIEKNSELTLTEISLYTGLAKSTTTRLLSTLEMNNFVEKD
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVNAKYRLGKQIYFLGFVAGQTFELNSLAKSTMERLREQTKETVNLYILDGKHRVCVQQF
CCCHHHHHCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
ESLQSVKHMISVGQKLPLTVGASGKVFLAYQSKEFIEDAMDTQPLKKSKVDLKNELDLII
HHHHHHHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHCCCCCHHHHCCHHHHHHHEE
KEKYAVSIEERESGTSAAAAPIFNFQNEVVAVLSVSGPASRLKPREWPELRKMVMEASVE
ECCEEEEEHHCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHH
ISKNLGYISNRAHEN
HHHCCCHHHHCCCCC
>Mature Secondary Structure
MNDSPKGIRTLQRSIDILNCFIEKNSELTLTEISLYTGLAKSTTTRLLSTLEMNNFVEKD
CCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHH
EVNAKYRLGKQIYFLGFVAGQTFELNSLAKSTMERLREQTKETVNLYILDGKHRVCVQQF
CCCHHHHHCCEEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
ESLQSVKHMISVGQKLPLTVGASGKVFLAYQSKEFIEDAMDTQPLKKSKVDLKNELDLII
HHHHHHHHHHHCCCCCCEEECCCCCEEEEECCHHHHHHHHCCCCCHHHHCCHHHHHHHEE
KEKYAVSIEERESGTSAAAAPIFNFQNEVVAVLSVSGPASRLKPREWPELRKMVMEASVE
ECCEEEEEHHCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHH
ISKNLGYISNRAHEN
HHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]