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The map label for this gene is paaJ [H]

Identifier: 23100127

GI number: 23100127

Start: 2742591

End: 2743742

Strand: Reverse

Name: paaJ [H]

Synonym: OB2672

Alternate gene names: 23100127

Gene position: 2743742-2742591 (Counterclockwise)

Preceding gene: 23100128

Following gene: 23100126

Centisome position: 75.57

GC content: 40.71

Gene sequence:

>1152_bases
TTGCGTGAAGCAGTAATAGTAGAAGCGGTGCGTACACCAGTCGGGAAGCGAAACGGTTCACTTAGTGAAATTAGAGCAGA
GGATTTGGCAGCGATGCCTTTAAAAGAGTTAGTAACTCGTACTGGATTGGATTCAGGATTAATTGAAGATGTCATTATGG
GTTGTGTCACCCAAAGTGGTGAACAAGCCTTTGATATAGGCAGGCAAGCTGCTTTAATCGCTGGATATCCGATAGAAGTT
CCAGGCACAACAATAGATAGACAATGTGGTTCGAGTCAACAAGCTATTCATTTTGCAGCTCAGGCAATTTTAAGCGGAGA
CATGGATATAGTTGTTGCTGCTGGAATAGAAAGTATGTCTCGTGTTCCGATGGGGTCAAATCAACAAGGAGTAAAGCTAA
GTAAATCATTGACGACGAAATATGAAATTATTAATCAAGGATTGTCGGCAGAAAGAATTGCAAAGCAATGGAATATTTCC
CGAGAAGAAATGGATGTATTTGCTTTAGAAAGCCATCAAAAAGCAATTGAAGCCAGAAACAATGGTTATTTTACAGAACA
ACTTATGTCAGTAGAGATCACAAGAGCTGATGGAACAAAAGTGAGCATTGATTCTGATGAAGGTCCAAGAGAAAATTCCT
CAATGGACAAATTAGCTGGATTATCATCGCCGTTTGAAAAGAATGGTAGTGTAACTGCTGGGAATGCGAGTCAAATTAGT
GATGGTGCAGCTGCATTATTGATTATGTCCCGAGAAAAGGCACAAGAATTGGGGTTAAAACCACGCTTTCGAATTATAGC
TCGTTCTGTAATTGGATCAGATCCAACATTAATGCTAACTGGTCCTGTCTCTGCTACAAAAAAGGTGCTTGAAAAAGCAA
ATGTAAAGTTAGAAGAAATCGATGTATTTGAAGTGAATGAAGCGTTTGCTTCTGTGCCTTTAATGTGGCTGAAAGAAACG
GGAGCGGATCCGAAGAAAATGAATCAAGATGGAGGTGCGATTGCATTAGGGCATCCTCTCGGTGCTAGTGGTGCCCGATT
AATGACTACTATGTTGCACACATTGGAAAGAACAGGTGGAAGATATGGTCTTCAAACGATGTGTGAAGGACATGGTATGG
CAAACGCCACCATTATCGAAAGATTGGATTAA

Upstream 100 bases:

>100_bases
CCTTTTTTCAGAGGGTGATTTGACAATGGTTGCAAGTTAAGAGTCAAGTGATAGACATGAATATTTTGGTACTAACCTGA
TCATTAAAGGAGGAGTTCGT

Downstream 100 bases:

>100_bases
TCGTATAAGAGGAGGAGCATTATGCCGCTTTCCAATATAAGAGTTTTAGATTTAACAAGACTACTACCCGGTCCTTATTG
TTCGATGATGCTAGCAGATT

Product: beta-ketothiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MREAVIVEAVRTPVGKRNGSLSEIRAEDLAAMPLKELVTRTGLDSGLIEDVIMGCVTQSGEQAFDIGRQAALIAGYPIEV
PGTTIDRQCGSSQQAIHFAAQAILSGDMDIVVAAGIESMSRVPMGSNQQGVKLSKSLTTKYEIINQGLSAERIAKQWNIS
REEMDVFALESHQKAIEARNNGYFTEQLMSVEITRADGTKVSIDSDEGPRENSSMDKLAGLSSPFEKNGSVTAGNASQIS
DGAAALLIMSREKAQELGLKPRFRIIARSVIGSDPTLMLTGPVSATKKVLEKANVKLEEIDVFEVNEAFASVPLMWLKET
GADPKKMNQDGGAIALGHPLGASGARLMTTMLHTLERTGGRYGLQTMCEGHGMANATIIERLD

Sequences:

>Translated_383_residues
MREAVIVEAVRTPVGKRNGSLSEIRAEDLAAMPLKELVTRTGLDSGLIEDVIMGCVTQSGEQAFDIGRQAALIAGYPIEV
PGTTIDRQCGSSQQAIHFAAQAILSGDMDIVVAAGIESMSRVPMGSNQQGVKLSKSLTTKYEIINQGLSAERIAKQWNIS
REEMDVFALESHQKAIEARNNGYFTEQLMSVEITRADGTKVSIDSDEGPRENSSMDKLAGLSSPFEKNGSVTAGNASQIS
DGAAALLIMSREKAQELGLKPRFRIIARSVIGSDPTLMLTGPVSATKKVLEKANVKLEEIDVFEVNEAFASVPLMWLKET
GADPKKMNQDGGAIALGHPLGASGARLMTTMLHTLERTGGRYGLQTMCEGHGMANATIIERLD
>Mature_383_residues
MREAVIVEAVRTPVGKRNGSLSEIRAEDLAAMPLKELVTRTGLDSGLIEDVIMGCVTQSGEQAFDIGRQAALIAGYPIEV
PGTTIDRQCGSSQQAIHFAAQAILSGDMDIVVAAGIESMSRVPMGSNQQGVKLSKSLTTKYEIINQGLSAERIAKQWNIS
REEMDVFALESHQKAIEARNNGYFTEQLMSVEITRADGTKVSIDSDEGPRENSSMDKLAGLSSPFEKNGSVTAGNASQIS
DGAAALLIMSREKAQELGLKPRFRIIARSVIGSDPTLMLTGPVSATKKVLEKANVKLEEIDVFEVNEAFASVPLMWLKET
GADPKKMNQDGGAIALGHPLGASGARLMTTMLHTLERTGGRYGLQTMCEGHGMANATIIERLD

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=38.974358974359, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI4557237, Length=394, Percent_Identity=39.0862944162437, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=36.9897959183673, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI4501853, Length=385, Percent_Identity=37.6623376623377, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=33.2552693208431, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI194353979, Length=384, Percent_Identity=28.6458333333333, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=44.6384039900249, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI48994986, Length=390, Percent_Identity=43.3333333333333, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI87082165, Length=393, Percent_Identity=40.4580152671756, Blast_Score=277, Evalue=8e-76,
Organism=Escherichia coli, GI1788554, Length=396, Percent_Identity=41.9191919191919, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1788683, Length=408, Percent_Identity=30.1470588235294, Blast_Score=180, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=38.0462724935733, Blast_Score=247, Evalue=6e-66,
Organism=Caenorhabditis elegans, GI25147385, Length=392, Percent_Identity=35.7142857142857, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=32.824427480916, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=33.3333333333333, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=30.4123711340206, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6322031, Length=387, Percent_Identity=37.4677002583979, Blast_Score=229, Evalue=6e-61,
Organism=Saccharomyces cerevisiae, GI6325229, Length=394, Percent_Identity=36.5482233502538, Blast_Score=221, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24655093, Length=393, Percent_Identity=42.7480916030534, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=36.9897959183673, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17648125, Length=388, Percent_Identity=37.6288659793814, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=33.2547169811321, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41050; Mature: 41050

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAVIVEAVRTPVGKRNGSLSEIRAEDLAAMPLKELVTRTGLDSGLIEDVIMGCVTQSG
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCC
EQAFDIGRQAALIAGYPIEVPGTTIDRQCGSSQQAIHFAAQAILSGDMDIVVAAGIESMS
CHHHHHCCCEEEEECCCEECCCCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHH
RVPMGSNQQGVKLSKSLTTKYEIINQGLSAERIAKQWNISREEMDVFALESHQKAIEARN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NGYFTEQLMSVEITRADGTKVSIDSDEGPRENSSMDKLAGLSSPFEKNGSVTAGNASQIS
CCEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCHHHCC
DGAAALLIMSREKAQELGLKPRFRIIARSVIGSDPTLMLTGPVSATKKVLEKANVKLEEI
CCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCEEEHE
DVFEVNEAFASVPLMWLKETGADPKKMNQDGGAIALGHPLGASGARLMTTMLHTLERTGG
EEEEHHHHHHHCCHHHHHHCCCCHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCC
RYGLQTMCEGHGMANATIIERLD
CCCHHHHHCCCCCCCHHHHEECC
>Mature Secondary Structure
MREAVIVEAVRTPVGKRNGSLSEIRAEDLAAMPLKELVTRTGLDSGLIEDVIMGCVTQSG
CCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHCCCC
EQAFDIGRQAALIAGYPIEVPGTTIDRQCGSSQQAIHFAAQAILSGDMDIVVAAGIESMS
CHHHHHCCCEEEEECCCEECCCCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCHHHHH
RVPMGSNQQGVKLSKSLTTKYEIINQGLSAERIAKQWNISREEMDVFALESHQKAIEARN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NGYFTEQLMSVEITRADGTKVSIDSDEGPRENSSMDKLAGLSSPFEKNGSVTAGNASQIS
CCEEEEEEEEEEEEECCCCEEEECCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCHHHCC
DGAAALLIMSREKAQELGLKPRFRIIARSVIGSDPTLMLTGPVSATKKVLEKANVKLEEI
CCCEEEEEEEHHHHHHCCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCEEEHE
DVFEVNEAFASVPLMWLKETGADPKKMNQDGGAIALGHPLGASGARLMTTMLHTLERTGG
EEEEHHHHHHHCCHHHHHHCCCCHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCC
RYGLQTMCEGHGMANATIIERLD
CCCHHHHHCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]