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The map label for this gene is yrkF [H]

Identifier: 23100106

GI number: 23100106

Start: 2719043

End: 2719621

Strand: Reverse

Name: yrkF [H]

Synonym: OB2651

Alternate gene names: 23100106

Gene position: 2719621-2719043 (Counterclockwise)

Preceding gene: 23100107

Following gene: 23100105

Centisome position: 74.91

GC content: 37.65

Gene sequence:

>579_bases
ATGGAATACTTGAAAACAGATCATCAAGTTGATGCTAAGGGATTAGCATGTCCAATGCCAATTGTAAAAACAAAAAAAGC
GATGAACAATGTGAAAGCAGGAGAAATAATTGAAATTCTCGCCACTGATCAAGGTTCGAAATCGGATATACAGGCATGGG
CAAAGAGTGCTGGACATCAATATCTTGGAACAATCGAAGAAGATAATGTACTCAAGCATTATTTACGAAAGACTACAGAG
GTGGAAAAAGAAGAAATGATATTTTCAAGAACAATAGATAACGATGAATTAACGAAAAAGTTAGTAGACTATCATACGGA
TCAATCCATATTAGTAGATGTTCGTGAGCAAGCAGAGTACGCTTTTGGCCATATTCCCCAGGCAGTATCTATTCCTTTAG
GAGAAATAGATAATCGAATGGACCAATTAGACAAAGACAAGACAATCTATGTTATTTGCCGAACTGGTACACGCAGCGGG
ATGGCAGCACAAAAGCTCATAGATAGCGGGTATGAGCATGTAATTAATGTGAAACCAGGTATGAGTCAATGGCAAGGGGA
TATAAGAAGCGAATTATAA

Upstream 100 bases:

>100_bases
TTATTAGATGACATCGAATATGCGGGTGTAGCTGCATATATTGGTGACGCTGAAGAGGGACAAATTAACTTATTTATATA
ATTTAAAGGAGGAATGTTTG

Downstream 100 bases:

>100_bases
GCCGACAAGGCATTTTATTTTTATTATATTAAATACGTATGGGGGTATGAAAATGATGAAAAAAATGAGTGCGCATGAAT
TAACACGACGCATTTTACAG

Product: hypothetical protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MEYLKTDHQVDAKGLACPMPIVKTKKAMNNVKAGEIIEILATDQGSKSDIQAWAKSAGHQYLGTIEEDNVLKHYLRKTTE
VEKEEMIFSRTIDNDELTKKLVDYHTDQSILVDVREQAEYAFGHIPQAVSIPLGEIDNRMDQLDKDKTIYVICRTGTRSG
MAAQKLIDSGYEHVINVKPGMSQWQGDIRSEL

Sequences:

>Translated_192_residues
MEYLKTDHQVDAKGLACPMPIVKTKKAMNNVKAGEIIEILATDQGSKSDIQAWAKSAGHQYLGTIEEDNVLKHYLRKTTE
VEKEEMIFSRTIDNDELTKKLVDYHTDQSILVDVREQAEYAFGHIPQAVSIPLGEIDNRMDQLDKDKTIYVICRTGTRSG
MAAQKLIDSGYEHVINVKPGMSQWQGDIRSEL
>Mature_192_residues
MEYLKTDHQVDAKGLACPMPIVKTKKAMNNVKAGEIIEILATDQGSKSDIQAWAKSAGHQYLGTIEEDNVLKHYLRKTTE
VEKEEMIFSRTIDNDELTKKLVDYHTDQSILVDVREQAEYAFGHIPQAVSIPLGEIDNRMDQLDKDKTIYVICRTGTRSG
MAAQKLIDSGYEHVINVKPGMSQWQGDIRSEL

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001455 [H]

Pfam domain/function: PF00581 Rhodanese; PF01206 SirA [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 21682; Mature: 21682

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50206 RHODANESE_3 ; PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYLKTDHQVDAKGLACPMPIVKTKKAMNNVKAGEIIEILATDQGSKSDIQAWAKSAGHQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCH
YLGTIEEDNVLKHYLRKTTEVEKEEMIFSRTIDNDELTKKLVDYHTDQSILVDVREQAEY
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AFGHIPQAVSIPLGEIDNRMDQLDKDKTIYVICRTGTRSGMAAQKLIDSGYEHVINVKPG
HHHCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCC
MSQWQGDIRSEL
HHHHHHHHHCCC
>Mature Secondary Structure
MEYLKTDHQVDAKGLACPMPIVKTKKAMNNVKAGEIIEILATDQGSKSDIQAWAKSAGHQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCH
YLGTIEEDNVLKHYLRKTTEVEKEEMIFSRTIDNDELTKKLVDYHTDQSILVDVREQAEY
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
AFGHIPQAVSIPLGEIDNRMDQLDKDKTIYVICRTGTRSGMAAQKLIDSGYEHVINVKPG
HHHCCCCHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEECCCC
MSQWQGDIRSEL
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]