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The map label for this gene is ilvB [H]

Identifier: 23100078

GI number: 23100078

Start: 2686337

End: 2688058

Strand: Reverse

Name: ilvB [H]

Synonym: OB2623

Alternate gene names: 23100078

Gene position: 2688058-2686337 (Counterclockwise)

Preceding gene: 23100079

Following gene: 23100077

Centisome position: 74.04

GC content: 39.9

Gene sequence:

>1722_bases
GTGAAAGTAGATGCAAACCAGCAGATGGAGGTTAAAAGTAATTTAGTAACGGGTGCTGATTTGTTTGTGCAAGCATTGGA
AAGAGCAAATGTGGAAGTAGTTTTTGGTTATCCTGGAGGTGCAGTATTACCGATTTACGATGCGTTACATCGGAATCAAA
CATCTTTTGAGCATGTACTTTCGCGACATGAGCAAGGATCCATTCATGCAGCAGAAGGTTATGCGAGAGTCTCTGGCAAA
CCAGGAGTTGTAATTGCTACTTCTGGACCGGGCGCGACAAATCTAATTACTGGGATAACGGATGCGATGATGGACTCTAT
ACCTCTTGTCATTTTTACTGGTCAAGTTGCAAAAGGAGTTATTGGAACAGATGCTTTTCAAGAAGCAGATGTAATGGGAA
TTACAACACCTATTACCAAATATAACTACCAAGTGAATGAAATAGCTGAACTGCCCCGTATTGTAAATGAAGCATTTCAC
ATTGCGACAACAGGTAGACCAGGGCCAGTCGTTGTAGATATTCCAAAGAATATTTCTTCGACAGTGACGGTGAATGATTA
TGAAGCGGATTTTCATTTACCTGGTTATCAGCCAACAATCACACCGAATCCTTTACAAATTACCAAAGTAAATGAAGCAT
TAAAACGAGCGGAGAAGCCGGTACTATTAGCTGGAGCGGGAATTCTTATCTCTGACGCTTCCAATGAATTAAAACAGTTT
GCTCAACATTATCAACTACCGGTAGTTACCACCTTACTTGGATTAGGAAGCTATCCTGGTAAGGAATCCTTATCATTAGG
AATGGCGGGGATGCACGGAACGTATGCAGCAAATATGGCAATCTATGAATGTGATTTGTTAATAAATATTGGTGCACGAT
TTGATGATAGGCTGACGGGAAATATTAAACACTTTGCACCAAATGCGAAGATCGTTCACATTGATATCGACCCTGCTGAA
ATTGGGAAAAATATCGAAACGAATATTCCTGTTGTTGCTGATGCAAAACGAGCATTAACAGCTCTTTTAAAAAATACAAC
AGAAAAATGTGATCATCAAAATTGGCTCCATCAGTTGAAGCATAATCAATTAGATTTCCCATTGTGGTATGATCGTTCCG
ATCAAGAAATCTCTCCCCAATGGTTAATGGAGCAAATATATGAGCTGTCTAAGGGAGAAGCAGTTGTAACAACAGATGTA
GGGCAGCATCAAATGTGGGCTGCACAGTATTACTCATTTAAAGATCCACATAATTGGGTGACATCTGGTGGATTAGGGAC
AATGGGGTTCGGCTTCCCTGCAGCCATTGGAGCTCAAATGGCTAAACCAAACAGTTTAGTTTTAGCAGTAGTTGGTGATG
GTGGTTTTCAAATGACTTTACAGGAATTGTCCATACTGAAATCACAGAATCTGCCAGTAAAAGTAATAATCTTAAATAAT
GAAGCGCTTGGTATGGTACGCCAATGGCAGGAAAGTTTTTACGAAGAAAGATATTCACATTCGCTGTTTTCAGAAAACCC
TGATTTTGTGAAATTGGCAGAAAGTTATGGTGTGAGAGGAATGAGAGTGGAGAAAGAAGCTGATGTTCCAAATATCTTAT
CTGAAGTATTTACCTACGATGGCCCAGTTGTAATTGATTGCAGAGTAGTGCAAAAAACCTCTGTTTATCCAATGATTGCA
CCAGGTACAGGAATCCAAGAAATGATTGGAGTGAGAAGATGA

Upstream 100 bases:

>100_bases
AAAGAAGACTCTTTTCATCCCATGCATGATAGAGAACATGTGTGTGGGGTGAGAAGGGTCTTTTTTTGTTAGCGAATAAT
AACTAAAGGAGGTTACTGTT

Downstream 100 bases:

>100_bases
AGAGAATTATCATCGCTACTGTACAAGATCGAGGGGGAGTCCTGAATCGCATTACTGGAATGCTTCACAAAAGGCAATTC
AACATTGAAAGTATTTCTGT

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKVDANQQMEVKSNLVTGADLFVQALERANVEVVFGYPGGAVLPIYDALHRNQTSFEHVLSRHEQGSIHAAEGYARVSGK
PGVVIATSGPGATNLITGITDAMMDSIPLVIFTGQVAKGVIGTDAFQEADVMGITTPITKYNYQVNEIAELPRIVNEAFH
IATTGRPGPVVVDIPKNISSTVTVNDYEADFHLPGYQPTITPNPLQITKVNEALKRAEKPVLLAGAGILISDASNELKQF
AQHYQLPVVTTLLGLGSYPGKESLSLGMAGMHGTYAANMAIYECDLLINIGARFDDRLTGNIKHFAPNAKIVHIDIDPAE
IGKNIETNIPVVADAKRALTALLKNTTEKCDHQNWLHQLKHNQLDFPLWYDRSDQEISPQWLMEQIYELSKGEAVVTTDV
GQHQMWAAQYYSFKDPHNWVTSGGLGTMGFGFPAAIGAQMAKPNSLVLAVVGDGGFQMTLQELSILKSQNLPVKVIILNN
EALGMVRQWQESFYEERYSHSLFSENPDFVKLAESYGVRGMRVEKEADVPNILSEVFTYDGPVVIDCRVVQKTSVYPMIA
PGTGIQEMIGVRR

Sequences:

>Translated_573_residues
MKVDANQQMEVKSNLVTGADLFVQALERANVEVVFGYPGGAVLPIYDALHRNQTSFEHVLSRHEQGSIHAAEGYARVSGK
PGVVIATSGPGATNLITGITDAMMDSIPLVIFTGQVAKGVIGTDAFQEADVMGITTPITKYNYQVNEIAELPRIVNEAFH
IATTGRPGPVVVDIPKNISSTVTVNDYEADFHLPGYQPTITPNPLQITKVNEALKRAEKPVLLAGAGILISDASNELKQF
AQHYQLPVVTTLLGLGSYPGKESLSLGMAGMHGTYAANMAIYECDLLINIGARFDDRLTGNIKHFAPNAKIVHIDIDPAE
IGKNIETNIPVVADAKRALTALLKNTTEKCDHQNWLHQLKHNQLDFPLWYDRSDQEISPQWLMEQIYELSKGEAVVTTDV
GQHQMWAAQYYSFKDPHNWVTSGGLGTMGFGFPAAIGAQMAKPNSLVLAVVGDGGFQMTLQELSILKSQNLPVKVIILNN
EALGMVRQWQESFYEERYSHSLFSENPDFVKLAESYGVRGMRVEKEADVPNILSEVFTYDGPVVIDCRVVQKTSVYPMIA
PGTGIQEMIGVRR
>Mature_573_residues
MKVDANQQMEVKSNLVTGADLFVQALERANVEVVFGYPGGAVLPIYDALHRNQTSFEHVLSRHEQGSIHAAEGYARVSGK
PGVVIATSGPGATNLITGITDAMMDSIPLVIFTGQVAKGVIGTDAFQEADVMGITTPITKYNYQVNEIAELPRIVNEAFH
IATTGRPGPVVVDIPKNISSTVTVNDYEADFHLPGYQPTITPNPLQITKVNEALKRAEKPVLLAGAGILISDASNELKQF
AQHYQLPVVTTLLGLGSYPGKESLSLGMAGMHGTYAANMAIYECDLLINIGARFDDRLTGNIKHFAPNAKIVHIDIDPAE
IGKNIETNIPVVADAKRALTALLKNTTEKCDHQNWLHQLKHNQLDFPLWYDRSDQEISPQWLMEQIYELSKGEAVVTTDV
GQHQMWAAQYYSFKDPHNWVTSGGLGTMGFGFPAAIGAQMAKPNSLVLAVVGDGGFQMTLQELSILKSQNLPVKVIILNN
EALGMVRQWQESFYEERYSHSLFSENPDFVKLAESYGVRGMRVEKEADVPNILSEVFTYDGPVVIDCRVVQKTSVYPMIA
PGTGIQEMIGVRR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=501, Percent_Identity=24.750499001996, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI21361361, Length=560, Percent_Identity=21.9642857142857, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=45.1327433628319, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=43.3691756272401, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=33.3333333333333, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=29.4545454545455, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI1788716, Length=499, Percent_Identity=24.6492985971944, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=26.056338028169, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=26.056338028169, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=609, Percent_Identity=24.1379310344828, Blast_Score=129, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=590, Percent_Identity=46.1016949152542, Blast_Score=521, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6320816, Length=493, Percent_Identity=22.3123732251521, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323163, Length=490, Percent_Identity=24.0816326530612, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=510, Percent_Identity=21.5686274509804, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=488, Percent_Identity=22.7459016393443, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=489, Percent_Identity=24.9488752556237, Blast_Score=155, Evalue=8e-38,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62764; Mature: 62764

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDANQQMEVKSNLVTGADLFVQALERANVEVVFGYPGGAVLPIYDALHRNQTSFEHVL
CCCCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHCCCCHHHHHH
SRHEQGSIHAAEGYARVSGKPGVVIATSGPGATNLITGITDAMMDSIPLVIFTGQVAKGV
HCCCCCCEEEECCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECHHHCCC
IGTDAFQEADVMGITTPITKYNYQVNEIAELPRIVNEAFHIATTGRPGPVVVDIPKNISS
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCC
TVTVNDYEADFHLPGYQPTITPNPLQITKVNEALKRAEKPVLLAGAGILISDASNELKQF
EEEEEECCCEEECCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEECCCEEEECCHHHHHHH
AQHYQLPVVTTLLGLGSYPGKESLSLGMAGMHGTYAANMAIYECDLLINIGARFDDRLTG
HHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCEEECEEEEEEEEEEECCCCCCCCCCC
NIKHFAPNAKIVHIDIDPAEIGKNIETNIPVVADAKRALTALLKNTTEKCDHQNWLHQLK
CCCEECCCCEEEEEECCHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
HNQLDFPLWYDRSDQEISPQWLMEQIYELSKGEAVVTTDVGQHQMWAAQYYSFKDPHNWV
CCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCC
TSGGLGTMGFGFPAAIGAQMAKPNSLVLAVVGDGGFQMTLQELSILKSQNLPVKVIILNN
CCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCEEEEEECC
EALGMVRQWQESFYEERYSHSLFSENPDFVKLAESYGVRGMRVEKEADVPNILSEVFTYD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCEEECCCCCCHHHHHHHHHCC
GPVVIDCRVVQKTSVYPMIAPGTGIQEMIGVRR
CCEEEEEEEEECCCCCEEECCCCCHHHHHCCCC
>Mature Secondary Structure
MKVDANQQMEVKSNLVTGADLFVQALERANVEVVFGYPGGAVLPIYDALHRNQTSFEHVL
CCCCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCEEHHHHHHHCCCCHHHHHH
SRHEQGSIHAAEGYARVSGKPGVVIATSGPGATNLITGITDAMMDSIPLVIFTGQVAKGV
HCCCCCCEEEECCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECHHHCCC
IGTDAFQEADVMGITTPITKYNYQVNEIAELPRIVNEAFHIATTGRPGPVVVDIPKNISS
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCEEEECCCCCCC
TVTVNDYEADFHLPGYQPTITPNPLQITKVNEALKRAEKPVLLAGAGILISDASNELKQF
EEEEEECCCEEECCCCCCCCCCCCEEEEHHHHHHHHCCCCEEEECCCEEEECCHHHHHHH
AQHYQLPVVTTLLGLGSYPGKESLSLGMAGMHGTYAANMAIYECDLLINIGARFDDRLTG
HHHCCCHHHHHHHHCCCCCCCCCCEECCCCCCCCEEECEEEEEEEEEEECCCCCCCCCCC
NIKHFAPNAKIVHIDIDPAEIGKNIETNIPVVADAKRALTALLKNTTEKCDHQNWLHQLK
CCCEECCCCEEEEEECCHHHHCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
HNQLDFPLWYDRSDQEISPQWLMEQIYELSKGEAVVTTDVGQHQMWAAQYYSFKDPHNWV
CCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCC
TSGGLGTMGFGFPAAIGAQMAKPNSLVLAVVGDGGFQMTLQELSILKSQNLPVKVIILNN
CCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEHHHHHHHHHCCCCCEEEEEECC
EALGMVRQWQESFYEERYSHSLFSENPDFVKLAESYGVRGMRVEKEADVPNILSEVFTYD
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCEEECCCCCCHHHHHHHHHCC
GPVVIDCRVVQKTSVYPMIAPGTGIQEMIGVRR
CCEEEEEEEEECCCCCEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]