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The map label for this gene is ilvN [H]

Identifier: 23100077

GI number: 23100077

Start: 2685816

End: 2686340

Strand: Reverse

Name: ilvN [H]

Synonym: OB2622

Alternate gene names: 23100077

Gene position: 2686340-2685816 (Counterclockwise)

Preceding gene: 23100078

Following gene: 23100076

Centisome position: 73.99

GC content: 36.38

Gene sequence:

>525_bases
ATGAAGAGAATTATCATCGCTACTGTACAAGATCGAGGGGGAGTCCTGAATCGCATTACTGGAATGCTTCACAAAAGGCA
ATTCAACATTGAAAGTATTTCTGTAGGAGCTTCAGAAACAGAAGGCATATCCAAAATGACATTTGTTGTTGAAATTAATG
ACAAACAAAAATTGGAACAATTAACAAAGCAATTAAATAAACAAATCGATGTATTAAAGGTTATGGATGTGACTGACAAA
GCCTTAGTTGCAAGAGAGTTGGCACTTATAAAGGTAGTCGGTACTAATCAAACAAGAGCGGAAATCCAAAGTATTATTAC
ACCTTTTCGAGCAGCGGTGATTGATGTAAGTAAAGATAGCTTAATCATCCAAGTAACTGGTAAACCAGATAAGGTGGATG
CGTTAATTGCTCTATTAAGACCATATGGAATTAAGGAACTGACAAAAACAGGCTTAACTGCATTTTTAAGAGGACAGCAG
CCAGAACAATCGATCAGTGATTTCAATCTAGTGGCGAAAGGTTAA

Upstream 100 bases:

>100_bases
ATGGCCCAGTTGTAATTGATTGCAGAGTAGTGCAAAAAACCTCTGTTTATCCAATGATTGCACCAGGTACAGGAATCCAA
GAAATGATTGGAGTGAGAAG

Downstream 100 bases:

>100_bases
TTTCGAAAATTGATTCACAATATAAAATAATTATAAACGGAGAGGGAGATTAAAATGGCAAAAGTATTATATGAAAAAGA
CATTCAAGACGGAGTATTGA

Product: acetolactate synthase small subunit

Products: NA

Alternate protein names: Acetohydroxy-acid synthase small subunit; AHAS; ALS [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKRIIIATVQDRGGVLNRITGMLHKRQFNIESISVGASETEGISKMTFVVEINDKQKLEQLTKQLNKQIDVLKVMDVTDK
ALVARELALIKVVGTNQTRAEIQSIITPFRAAVIDVSKDSLIIQVTGKPDKVDALIALLRPYGIKELTKTGLTAFLRGQQ
PEQSISDFNLVAKG

Sequences:

>Translated_174_residues
MKRIIIATVQDRGGVLNRITGMLHKRQFNIESISVGASETEGISKMTFVVEINDKQKLEQLTKQLNKQIDVLKVMDVTDK
ALVARELALIKVVGTNQTRAEIQSIITPFRAAVIDVSKDSLIIQVTGKPDKVDALIALLRPYGIKELTKTGLTAFLRGQQ
PEQSISDFNLVAKG
>Mature_174_residues
MKRIIIATVQDRGGVLNRITGMLHKRQFNIESISVGASETEGISKMTFVVEINDKQKLEQLTKQLNKQIDVLKVMDVTDK
ALVARELALIKVVGTNQTRAEIQSIITPFRAAVIDVSKDSLIIQVTGKPDKVDALIALLRPYGIKELTKTGLTAFLRGQQ
PEQSISDFNLVAKG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0440

COG function: function code E; Acetolactate synthase, small (regulatory) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1786266, Length=161, Percent_Identity=32.9192546583851, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004789
- InterPro:   IPR019455
- InterPro:   IPR002912 [H]

Pfam domain/function: PF01842 ACT; PF10369 ALS_ss_C [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 19198; Mature: 19198

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIIIATVQDRGGVLNRITGMLHKRQFNIESISVGASETEGISKMTFVVEINDKQKLEQ
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCHHHHHH
LTKQLNKQIDVLKVMDVTDKALVARELALIKVVGTNQTRAEIQSIITPFRAAVIDVSKDS
HHHHHHHHHCCCEEECCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHEEEECCCC
LIIQVTGKPDKVDALIALLRPYGIKELTKTGLTAFLRGQQPEQSISDFNLVAKG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHCCCEEEECC
>Mature Secondary Structure
MKRIIIATVQDRGGVLNRITGMLHKRQFNIESISVGASETEGISKMTFVVEINDKQKLEQ
CCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCHHHHHH
LTKQLNKQIDVLKVMDVTDKALVARELALIKVVGTNQTRAEIQSIITPFRAAVIDVSKDS
HHHHHHHHHCCCEEECCCHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHEEEECCCC
LIIQVTGKPDKVDALIALLRPYGIKELTKTGLTAFLRGQQPEQSISDFNLVAKG
EEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]