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The map label for this gene is leuD

Identifier: 23100072

GI number: 23100072

Start: 2680061

End: 2680639

Strand: Reverse

Name: leuD

Synonym: OB2617

Alternate gene names: 23100072

Gene position: 2680639-2680061 (Counterclockwise)

Preceding gene: 23100073

Following gene: 23100071

Centisome position: 73.84

GC content: 36.61

Gene sequence:

>579_bases
ATGGAACCATTGGAATTTCATAGTGGAAAGGTCTGTCCATTAAATAGAGCTAATGTAGACACGGACCAAATTATTCCAAA
GCAATTTTTAAAACGAATCGAACGAACTGGTTTTGGACAGTTTCTATTTTTTCATTGGAGGTTCAATGATGATGGAACAG
AGCGAAGTGATTTTGTTTTAAATGATCCGGACTATAAAGGTTCTAGCATTTTAGTTGCCGGAGATAATTTTGGTTGTGGA
TCCAGTCGGGAACATGCTCCTTGGGCCCTTGCTGATTATGGATTTAAAGTGATTATTGCACCAGACTTTGCCGATATTTT
TTATAATAACGCGTTAAAAAATGGCATATTAGTTATTAAGATGGATGAGCAGCAAGTGAAGTCGTGGATGCAAAAAGCAG
TAGATGGGTTAACATTAGATGTTGATTTAAAGCAACAAACGATTCAAGCCTCTGATGGAACAATAACATCATTTGATATT
ACGCCGTACCAACGTGAAAAATTAATCAATGGTTGGGATGATATAGCCTTAACCTTATTAAAGGAAGAAAAGATTGCGGC
CTATGAATCTGCTAACTGA

Upstream 100 bases:

>100_bases
CATCTCGTCAGTCCAGAAATGGCTGCAGCTGCGGCAATAGAAGGACACTTTGTTGATGTTAGATCCTACGCTAGCGTCCC
GAGTTAGGAGGAGATCTTAA

Downstream 100 bases:

>100_bases
GATTTATTCCGCTGAATAAAGTTTCATTTTTTCGTTACAGAATAGAAGGAGAGTGAGTTGTCTTGGGGATCGCTTAACTG
GTGAAAAAGTATACCAAGCT

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MEPLEFHSGKVCPLNRANVDTDQIIPKQFLKRIERTGFGQFLFFHWRFNDDGTERSDFVLNDPDYKGSSILVAGDNFGCG
SSREHAPWALADYGFKVIIAPDFADIFYNNALKNGILVIKMDEQQVKSWMQKAVDGLTLDVDLKQQTIQASDGTITSFDI
TPYQREKLINGWDDIALTLLKEEKIAAYESAN

Sequences:

>Translated_192_residues
MEPLEFHSGKVCPLNRANVDTDQIIPKQFLKRIERTGFGQFLFFHWRFNDDGTERSDFVLNDPDYKGSSILVAGDNFGCG
SSREHAPWALADYGFKVIIAPDFADIFYNNALKNGILVIKMDEQQVKSWMQKAVDGLTLDVDLKQQTIQASDGTITSFDI
TPYQREKLINGWDDIALTLLKEEKIAAYESAN
>Mature_192_residues
MEPLEFHSGKVCPLNRANVDTDQIIPKQFLKRIERTGFGQFLFFHWRFNDDGTERSDFVLNDPDYKGSSILVAGDNFGCG
SSREHAPWALADYGFKVIIAPDFADIFYNNALKNGILVIKMDEQQVKSWMQKAVDGLTLDVDLKQQTIQASDGTITSFDI
TPYQREKLINGWDDIALTLLKEEKIAAYESAN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=188, Percent_Identity=53.7234042553192, Blast_Score=201, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=197, Percent_Identity=51.7766497461929, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_OCEIH (Q8EN70)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693538.1
- ProteinModelPortal:   Q8EN70
- SMR:   Q8EN70
- GeneID:   1015181
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2617
- NMPDR:   fig|221109.1.peg.2616
- HOGENOM:   HBG304838
- OMA:   DEISITM
- BioCyc:   OIHE221109:OB2617-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21761; Mature: 21761

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPLEFHSGKVCPLNRANVDTDQIIPKQFLKRIERTGFGQFLFFHWRFNDDGTERSDFVL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEE
NDPDYKGSSILVAGDNFGCGSSREHAPWALADYGFKVIIAPDFADIFYNNALKNGILVIK
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHCCCEEEEE
MDEQQVKSWMQKAVDGLTLDVDLKQQTIQASDGTITSFDITPYQREKLINGWDDIALTLL
ECHHHHHHHHHHHHCCEEEEEECCHHEEECCCCEEEEEECCHHHHHHHCCCCCHHEEEEH
KEEKIAAYESAN
HHCHHCEECCCC
>Mature Secondary Structure
MEPLEFHSGKVCPLNRANVDTDQIIPKQFLKRIERTGFGQFLFFHWRFNDDGTERSDFVL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEE
NDPDYKGSSILVAGDNFGCGSSREHAPWALADYGFKVIIAPDFADIFYNNALKNGILVIK
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHHHHHCCCEEEEE
MDEQQVKSWMQKAVDGLTLDVDLKQQTIQASDGTITSFDITPYQREKLINGWDDIALTLL
ECHHHHHHHHHHHHCCEEEEEECCHHEEECCCCEEEEEECCHHHHHHHCCCCCHHEEEEH
KEEKIAAYESAN
HHCHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376