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The map label for this gene is dhaK [H]

Identifier: 23099929

GI number: 23099929

Start: 2531087

End: 2532079

Strand: Reverse

Name: dhaK [H]

Synonym: OB2474

Alternate gene names: 23099929

Gene position: 2532079-2531087 (Counterclockwise)

Preceding gene: 23099930

Following gene: 23099928

Centisome position: 69.74

GC content: 37.16

Gene sequence:

>993_bases
ATGAAAAAAATTATTAATAATCCTAATGACGTTGTATCAGATATGGTTTCTGGGATGATACAGGCCTTTCCAGAAGAACT
AGAACAAATAGATGGAACAATGGTAATTAAACGTAAGGAATTATCTTCAAATAAAGTCGGTCTTGTTAGTGGTGGAGGAA
GCGGTCACGAACCAGCGCATGCTGGATATGTTGGAAAGGGAATGTTAGATGCAGCAGTTGCTGGTGAAGTATTTACTTCA
CCTACTCCTGATCAAATATTGGAAGCAATTAAAGCAGTAGATACTGGTCATGGAGTTTTATTAATTGTAAAAAACTATAC
AGGAGATGTTATGAATTTCGATATGGCTAGTGAATTGGCTGAGTCAGAGGGAATAAAGGTTGAACAAGTTATTGTTAACG
ATGATGTTGCGGTAGAAGATAGTGACTTTACTACTGGTCGAAGAGGTATTGCTGGTACTGTGTTTGTACACAAAATCGCT
GGAGCAATGGCTGAGACAGGTGCTACTTTAAATGAAGTGAAGGAAGTTGCTGAAAAAACAATTAATCAAGTCAGATCAAT
GGGGATGGCACTTACTCCATGTACAGTACCTGCTGCAGGTAAACCAAGTTTTCAATTAGAAGAAAATGAAATGGAGATTG
GTATAGGGATACATGGGGAAGCAGGTATAGAGAGAAAACCAATAGCATCTGCTGAAGATATTGCCAATGAACTAACGCAA
AAAGTATTTGATGATCTGGGATTACAATCAGGTGATGAGGTTGCAGTGATGATTAATGGATTAGGTGCAACTCCAGAGAT
GGAATTATTTATATTACAGAAACATGTAAATCATTTGCTTGAAAATAAACGTTTATCTGTGTTTCGTACGTATGTAGGTG
AGTTTATGACATCTCTAGAAATGACAGGTTGCTCGGTCACTTTATTGAAATTGGACGATCAATTAAAATCTCTTTTAGAA
GCAGCTAGTGAAGCTACAGCATTTCGTGTATAA

Upstream 100 bases:

>100_bases
GATTGTTTGACCATTTTCAAACTTCCCCTTAAAATATAGCGAACATTTGTTACATTTCTTAAGTAGCGTCCTACATTATG
AAAAGGAGAGATAAAATCTT

Downstream 100 bases:

>100_bases
GGAAAGGAGAATCTTCATTGAAATTACAGTTGAATGACATCAGAAATTGGCTGGAATTATCAAACCAATTAATGCAAGAG
AACAGATCCTATCTTATAGA

Product: dihydroxyacetone kinase

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKIINNPNDVVSDMVSGMIQAFPEELEQIDGTMVIKRKELSSNKVGLVSGGGSGHEPAHAGYVGKGMLDAAVAGEVFTS
PTPDQILEAIKAVDTGHGVLLIVKNYTGDVMNFDMASELAESEGIKVEQVIVNDDVAVEDSDFTTGRRGIAGTVFVHKIA
GAMAETGATLNEVKEVAEKTINQVRSMGMALTPCTVPAAGKPSFQLEENEMEIGIGIHGEAGIERKPIASAEDIANELTQ
KVFDDLGLQSGDEVAVMINGLGATPEMELFILQKHVNHLLENKRLSVFRTYVGEFMTSLEMTGCSVTLLKLDDQLKSLLE
AASEATAFRV

Sequences:

>Translated_330_residues
MKKIINNPNDVVSDMVSGMIQAFPEELEQIDGTMVIKRKELSSNKVGLVSGGGSGHEPAHAGYVGKGMLDAAVAGEVFTS
PTPDQILEAIKAVDTGHGVLLIVKNYTGDVMNFDMASELAESEGIKVEQVIVNDDVAVEDSDFTTGRRGIAGTVFVHKIA
GAMAETGATLNEVKEVAEKTINQVRSMGMALTPCTVPAAGKPSFQLEENEMEIGIGIHGEAGIERKPIASAEDIANELTQ
KVFDDLGLQSGDEVAVMINGLGATPEMELFILQKHVNHLLENKRLSVFRTYVGEFMTSLEMTGCSVTLLKLDDQLKSLLE
AASEATAFRV
>Mature_330_residues
MKKIINNPNDVVSDMVSGMIQAFPEELEQIDGTMVIKRKELSSNKVGLVSGGGSGHEPAHAGYVGKGMLDAAVAGEVFTS
PTPDQILEAIKAVDTGHGVLLIVKNYTGDVMNFDMASELAESEGIKVEQVIVNDDVAVEDSDFTTGRRGIAGTVFVHKIA
GAMAETGATLNEVKEVAEKTINQVRSMGMALTPCTVPAAGKPSFQLEENEMEIGIGIHGEAGIERKPIASAEDIANELTQ
KVFDDLGLQSGDEVAVMINGLGATPEMELFILQKHVNHLLENKRLSVFRTYVGEFMTSLEMTGCSVTLLKLDDQLKSLLE
AASEATAFRV

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=340, Percent_Identity=42.0588235294118, Blast_Score=256, Evalue=2e-68,
Organism=Escherichia coli, GI87081857, Length=352, Percent_Identity=45.1704545454545, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17565018, Length=328, Percent_Identity=35.3658536585366, Blast_Score=217, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6321055, Length=321, Percent_Identity=39.2523364485981, Blast_Score=208, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323570, Length=326, Percent_Identity=39.8773006134969, Blast_Score=198, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 35238; Mature: 35238

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIINNPNDVVSDMVSGMIQAFPEELEQIDGTMVIKRKELSSNKVGLVSGGGSGHEPAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCC
AGYVGKGMLDAAVAGEVFTSPTPDQILEAIKAVDTGHGVLLIVKNYTGDVMNFDMASELA
CCCCCCCHHHHHHHCHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ESEGIKVEQVIVNDDVAVEDSDFTTGRRGIAGTVFVHKIAGAMAETGATLNEVKEVAEKT
HHCCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
INQVRSMGMALTPCTVPAAGKPSFQLEENEMEIGIGIHGEAGIERKPIASAEDIANELTQ
HHHHHHCCCEECCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
KVFDDLGLQSGDEVAVMINGLGATPEMELFILQKHVNHLLENKRLSVFRTYVGEFMTSLE
HHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MTGCSVTLLKLDDQLKSLLEAASEATAFRV
HCCCEEEEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIINNPNDVVSDMVSGMIQAFPEELEQIDGTMVIKRKELSSNKVGLVSGGGSGHEPAH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEECCCCCCCCCC
AGYVGKGMLDAAVAGEVFTSPTPDQILEAIKAVDTGHGVLLIVKNYTGDVMNFDMASELA
CCCCCCCHHHHHHHCHHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ESEGIKVEQVIVNDDVAVEDSDFTTGRRGIAGTVFVHKIAGAMAETGATLNEVKEVAEKT
HHCCCEEEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
INQVRSMGMALTPCTVPAAGKPSFQLEENEMEIGIGIHGEAGIERKPIASAEDIANELTQ
HHHHHHCCCEECCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
KVFDDLGLQSGDEVAVMINGLGATPEMELFILQKHVNHLLENKRLSVFRTYVGEFMTSLE
HHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MTGCSVTLLKLDDQLKSLLEAASEATAFRV
HCCCEEEEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]