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The map label for this gene is glpK

Identifier: 23099930

GI number: 23099930

Start: 2532254

End: 2533756

Strand: Reverse

Name: glpK

Synonym: OB2475

Alternate gene names: 23099930

Gene position: 2533756-2532254 (Counterclockwise)

Preceding gene: 23099931

Following gene: 23099929

Centisome position: 69.79

GC content: 37.46

Gene sequence:

>1503_bases
ATGTCAGAAAAATTTATTTTGTCCATTGATCAAGGAACAACAAGCTCTCGAGCAATACTTTTTAATCATAAGGGTGAGAT
CGTTGAATCTGCTCAGAAGGAATTTGAGCAATTCTTCCCAAAACCAGGTTGGGTGGAACACGATGCAAATGAAATTTGGA
CGTCTGTATTAGCTTGTATTGCTGATGTTTTAAGAAAAGCAGATATAGAAGCTAACCAAATTGAAGGCATTGGAATCACC
AATCAACGTGAAACAACGGTAGTTTGGGATAAAAATACGGGTAGACCAATTTATAAGGCCATTGTTTGGCAATCTCGTCA
AACGGAAGGCATTTGTAAGGAACTAAAAGAACAAGGACATGAAGATACGATTCGTCATAAGACAGGTTTACTAATCGATC
CTTATTTCTCTGGTACAAAAGTAAAATGGATTTTAGATAATGTAGACGGAGCAAGAGAAAAAGCAGAAGCTGGAGAATTA
TCATTTGGAACGATAGATAGTTGGTTAATTTATCGATTATCTGGTGGTAAAACACATGTAACAGATTATTCCAATGCTTC
TCGTACATTAATGTTTAATATTTATGATCTTAAATGGGATGATGAGTTATTAGAAATATTGGATGTACCGAAAAGTATGC
TTCCTGAAGTAAAACCTTCTTCTGAAGTATACGCAAATACTGTTTCTTATCATTTCTTTGGTGAAGAAGTACCTATTGCA
GGTGTTGCTGGAGACCAACAAGCTGCTTTATTTGGTCAAGCTTGTTTCGATCAGGGAATGGCTAAAAACACGTATGGTAC
TGGTTGTTTCTTACTAATGAATACAGGTGAAGAACCAGTACGCTCTAAGCATGGTCTACTCACTACGATTGCTTGGGGAG
TAGATGGTAAAGTTGAGTATGCACTTGAAGGTAGTATTTTCGTTGCTGGATCTGCTATTCAGTGGCTTAGAGATGGATTA
AGACTTATTGAATCAAGTCCACAAAGTGAAGCATTAGCATCGCAAGTCGAGACAACAGATGGCGTGTATTTGGTGCCAGC
ATTTGTTGGTCTTGGAACACCTTACTGGGATAGTGATGCTCGAGGAGCTGTATTTGGCTTAACTCGTGGTACAACGAAGG
AACACTTTGTTCGTGCAACATTAGAATCTTTAGCATATCAAACAAAAGATGTTATGGATGCAATGTTAGCGGATTCTGAC
ATTGATTTGAAGAAATTACGTGTTGATGGTGGTGCAGTAAAAAATAATTTACTGATGCAGTTCCAAAGTGATATTCTGGG
AGTAACCGTTGAACGACCTGTCGTTCAAGAAACAACTGCACTAGGATCGGCTTATTTAGCTGGTTTAGCTGTAGGGTTCT
GGAAAGATAAAGAGGAAATTGCAAAACAATGGTTAAATGAGAAAACATTTGAGAATGAAATGAGTGAAGAGGAAAGTTCA
TACCTCTATAAAGGTTGGCAAAAAGCTGTTGAAGCAACTCGTTCATTTAAATTTGAATCATAA

Upstream 100 bases:

>100_bases
TGTAATAGTATTATTTATAGGAGCAGCAGTGAGTGAGTTGAAAACGGGTTCAAATCCTGCTGAAAAAATGAATCAAAAAT
TAAGATAGGAGGAATTTGGC

Downstream 100 bases:

>100_bases
AAAAGAAAGAAAACTGTCTCAATGAAGAGGCAGTTTTCTTGTACATGGATAAGAAGGATACGTGACCTTAGTCAGATTGT
TTGACCATTTTCAAACTTCC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSEKFILSIDQGTTSSRAILFNHKGEIVESAQKEFEQFFPKPGWVEHDANEIWTSVLACIADVLRKADIEANQIEGIGIT
NQRETTVVWDKNTGRPIYKAIVWQSRQTEGICKELKEQGHEDTIRHKTGLLIDPYFSGTKVKWILDNVDGAREKAEAGEL
SFGTIDSWLIYRLSGGKTHVTDYSNASRTLMFNIYDLKWDDELLEILDVPKSMLPEVKPSSEVYANTVSYHFFGEEVPIA
GVAGDQQAALFGQACFDQGMAKNTYGTGCFLLMNTGEEPVRSKHGLLTTIAWGVDGKVEYALEGSIFVAGSAIQWLRDGL
RLIESSPQSEALASQVETTDGVYLVPAFVGLGTPYWDSDARGAVFGLTRGTTKEHFVRATLESLAYQTKDVMDAMLADSD
IDLKKLRVDGGAVKNNLLMQFQSDILGVTVERPVVQETTALGSAYLAGLAVGFWKDKEEIAKQWLNEKTFENEMSEEESS
YLYKGWQKAVEATRSFKFES

Sequences:

>Translated_500_residues
MSEKFILSIDQGTTSSRAILFNHKGEIVESAQKEFEQFFPKPGWVEHDANEIWTSVLACIADVLRKADIEANQIEGIGIT
NQRETTVVWDKNTGRPIYKAIVWQSRQTEGICKELKEQGHEDTIRHKTGLLIDPYFSGTKVKWILDNVDGAREKAEAGEL
SFGTIDSWLIYRLSGGKTHVTDYSNASRTLMFNIYDLKWDDELLEILDVPKSMLPEVKPSSEVYANTVSYHFFGEEVPIA
GVAGDQQAALFGQACFDQGMAKNTYGTGCFLLMNTGEEPVRSKHGLLTTIAWGVDGKVEYALEGSIFVAGSAIQWLRDGL
RLIESSPQSEALASQVETTDGVYLVPAFVGLGTPYWDSDARGAVFGLTRGTTKEHFVRATLESLAYQTKDVMDAMLADSD
IDLKKLRVDGGAVKNNLLMQFQSDILGVTVERPVVQETTALGSAYLAGLAVGFWKDKEEIAKQWLNEKTFENEMSEEESS
YLYKGWQKAVEATRSFKFES
>Mature_499_residues
SEKFILSIDQGTTSSRAILFNHKGEIVESAQKEFEQFFPKPGWVEHDANEIWTSVLACIADVLRKADIEANQIEGIGITN
QRETTVVWDKNTGRPIYKAIVWQSRQTEGICKELKEQGHEDTIRHKTGLLIDPYFSGTKVKWILDNVDGAREKAEAGELS
FGTIDSWLIYRLSGGKTHVTDYSNASRTLMFNIYDLKWDDELLEILDVPKSMLPEVKPSSEVYANTVSYHFFGEEVPIAG
VAGDQQAALFGQACFDQGMAKNTYGTGCFLLMNTGEEPVRSKHGLLTTIAWGVDGKVEYALEGSIFVAGSAIQWLRDGLR
LIESSPQSEALASQVETTDGVYLVPAFVGLGTPYWDSDARGAVFGLTRGTTKEHFVRATLESLAYQTKDVMDAMLADSDI
DLKKLRVDGGAVKNNLLMQFQSDILGVTVERPVVQETTALGSAYLAGLAVGFWKDKEEIAKQWLNEKTFENEMSEEESSY
LYKGWQKAVEATRSFKFES

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=496, Percent_Identity=46.7741935483871, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI42794763, Length=500, Percent_Identity=46.6, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4504007, Length=496, Percent_Identity=46.7741935483871, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI41393575, Length=496, Percent_Identity=46.1693548387097, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI88196792, Length=503, Percent_Identity=33.9960238568588, Blast_Score=316, Evalue=3e-86,
Organism=Escherichia coli, GI1790361, Length=489, Percent_Identity=61.758691206544, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1789987, Length=505, Percent_Identity=24.7524752475248, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787789, Length=511, Percent_Identity=23.8747553816047, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789168, Length=476, Percent_Identity=23.5294117647059, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=449, Percent_Identity=43.4298440979955, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=525, Percent_Identity=26.2857142857143, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=30.5647840531561, Blast_Score=137, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=546, Percent_Identity=36.996336996337, Blast_Score=324, Evalue=2e-89,
Organism=Drosophila melanogaster, GI17864214, Length=514, Percent_Identity=44.5525291828794, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24654573, Length=462, Percent_Identity=44.5887445887446, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24655555, Length=496, Percent_Identity=38.508064516129, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655560, Length=496, Percent_Identity=38.508064516129, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655570, Length=496, Percent_Identity=38.508064516129, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655574, Length=496, Percent_Identity=38.508064516129, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655565, Length=496, Percent_Identity=38.508064516129, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI19922058, Length=521, Percent_Identity=30.7101727447217, Blast_Score=266, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24652871, Length=488, Percent_Identity=31.3524590163934, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24656367, Length=499, Percent_Identity=26.6533066132265, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI21357867, Length=510, Percent_Identity=25.2941176470588, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24656372, Length=331, Percent_Identity=29.0030211480363, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45552909, Length=290, Percent_Identity=27.5862068965517, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_OCEIH (Q8ENK7)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693396.1
- ProteinModelPortal:   Q8ENK7
- SMR:   Q8ENK7
- GeneID:   1015368
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2475
- NMPDR:   fig|221109.1.peg.2474
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   PRK00047
- BioCyc:   OIHE221109:OB2475-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55668; Mature: 55537

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 13-13 BINDING 17-17 BINDING 83-83 BINDING 135-135 BINDING 245-245 BINDING 267-267 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKFILSIDQGTTSSRAILFNHKGEIVESAQKEFEQFFPKPGWVEHDANEIWTSVLACI
CCCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ADVLRKADIEANQIEGIGITNQRETTVVWDKNTGRPIYKAIVWQSRQTEGICKELKEQGH
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
EDTIRHKTGLLIDPYFSGTKVKWILDNVDGAREKAEAGELSFGTIDSWLIYRLSGGKTHV
HHHHHHCCCCEECCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCEEEE
TDYSNASRTLMFNIYDLKWDDELLEILDVPKSMLPEVKPSSEVYANTVSYHFFGEEVPIA
ECCCCCCEEEEEEEEECCCCHHHHHHHHCCHHHCCCCCCCCHHHHEEEEEEEECCCCCEE
GVAGDQQAALFGQACFDQGMAKNTYGTGCFLLMNTGEEPVRSKHGLLTTIAWGVDGKVEY
ECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHCCCEEEEEEECCCCEEEE
ALEGSIFVAGSAIQWLRDGLRLIESSPQSEALASQVETTDGVYLVPAFVGLGTPYWDSDA
EECCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCC
RGAVFGLTRGTTKEHFVRATLESLAYQTKDVMDAMLADSDIDLKKLRVDGGAVKNNLLMQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
FQSDILGVTVERPVVQETTALGSAYLAGLAVGFWKDKEEIAKQWLNEKTFENEMSEEESS
HHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHH
YLYKGWQKAVEATRSFKFES
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEKFILSIDQGTTSSRAILFNHKGEIVESAQKEFEQFFPKPGWVEHDANEIWTSVLACI
CCCEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ADVLRKADIEANQIEGIGITNQRETTVVWDKNTGRPIYKAIVWQSRQTEGICKELKEQGH
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
EDTIRHKTGLLIDPYFSGTKVKWILDNVDGAREKAEAGELSFGTIDSWLIYRLSGGKTHV
HHHHHHCCCCEECCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEEECCCEEEE
TDYSNASRTLMFNIYDLKWDDELLEILDVPKSMLPEVKPSSEVYANTVSYHFFGEEVPIA
ECCCCCCEEEEEEEEECCCCHHHHHHHHCCHHHCCCCCCCCHHHHEEEEEEEECCCCCEE
GVAGDQQAALFGQACFDQGMAKNTYGTGCFLLMNTGEEPVRSKHGLLTTIAWGVDGKVEY
ECCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHCCCEEEEEEECCCCEEEE
ALEGSIFVAGSAIQWLRDGLRLIESSPQSEALASQVETTDGVYLVPAFVGLGTPYWDSDA
EECCCEEEECHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCC
RGAVFGLTRGTTKEHFVRATLESLAYQTKDVMDAMLADSDIDLKKLRVDGGAVKNNLLMQ
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
FQSDILGVTVERPVVQETTALGSAYLAGLAVGFWKDKEEIAKQWLNEKTFENEMSEEESS
HHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHH
YLYKGWQKAVEATRSFKFES
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376