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The map label for this gene is pykA [H]

Identifier: 23099626

GI number: 23099626

Start: 2205640

End: 2207400

Strand: Reverse

Name: pykA [H]

Synonym: OB2171

Alternate gene names: 23099626

Gene position: 2207400-2205640 (Counterclockwise)

Preceding gene: 23099627

Following gene: 23099625

Centisome position: 60.8

GC content: 37.59

Gene sequence:

>1761_bases
ATGAGAAACACAAAAATCGTATGTACAATTGGTCCTGCATCAGAATCAATTGAAAAGTTAGAGCAACTTATTGAAGCAGG
AATGAACGTAGCTCGTCTCAACTTTTCTCATGGAGATTATGCAGAGCATGGCGCACGGATTGAAAATATCCGAAAAGCAG
CTAAAAATAAAAATAAAACAGTAGCAATTCTTTTAGATACTAAAGGACCAGAGATTCGTACTGGTAGCTTTAAAGAAGGT
AGAGCAGATCTTATGCAGGGCAACCCTGTAACAATCGCTATGGATGAAGTAGAGGGGACTGCTGAGAAATTCTCTGTTAC
ATACCAAGGGCTAATCAATGATGTACATGAAGGTTCAAAGATTCTTTTGGATGATGGACTTATTGAATTAGAAGTAAAAT
CTATTGATAAAAGTAAAGGTGAAATTGAAACTATTGCACTTAACTCTGGGGAAATAAAGAATAAAAAAGGTGTTAACGTT
CCTAATGTTTCTGTAAATCTACCCGGTATAACAGAAAAAGACACCAATGATATTATCTTTGGAATTGAACAGGGTGTTGA
TTTTATTGCTGCATCTTTTGTGCGTCGAGCTTCGGATATTTTAGAAATTAAAGAAGTGCTAGATCAGCATCAGGCACAGT
ATATAAAAATCATCCCTAAAATTGAGAACCAAGAAGGTGTCGATAATATCGATAGTATTCTTGAGGTAAGTGATGGATTA
ATGGTAGCTCGTGGTGATTTAGGAGTGGAGATCCCAGCTGAAGATGTACCATTGGTACAGAAAAAATTAATTAAGCAATG
TAATAATGCTGGAAAGCCAGTAATTACTGCTACACAAATGTTAGATTCGATGCAGCGTAATCCACGACCTACACGTGCAG
AAGCATCAGACGTTGCTAATGCGATTTTTGATGGCACAGATGCAATTATGCTTTCTGGAGAAACTGCTGCGGGAACCTAC
CCAGTAGAATCTGTACAAACAATGAGTAATATTGCACTAAAAGCTGAATCTGCATTAGATCATAAACAAATGTTAGAAGA
ACGTTCACAATATGTCGATATGACTATAACAGAAGCAATTAGTCAATCCGTTACGCACACAGCATCCAATTTAAATGTTG
ATACAATTATCACACCGACAGAAAGCGGACATACAGCTAGAATGATTTCTAAATACCGTCCGAAAGCTTCCATTCTAGCA
GTTACTTTTAACGAAGTAGTAAATCGTCAACTATCCTTAGTTTGGGGAGTGCATGCTGTTCTGGGTAAACCAGCGACGTC
TACTGACGAAATGCTAGAAATTGCAGTTGATAAAGGATTAAATACAGGAATCTGCAGTAGAGGAAGCCGTGTCATTATTA
CGGCTGGTGTACCTGTTGGAGAGAGTGGAACAACTAACCTGTTAAAAGTTCATGTGATTGGTGATGTGATTGCTAAAGGA
CAAGGAATTGGAAGAAGAAGTGCATATGGACATGCCGTATTTGCTAAAACGAATAAAGAAGCTTTAGAAAAAGTACAAGA
CGGTGATATTTTAGTAACCTATGCAACAGAAAAAGAAATGATGCCTGCAATTGAAAAAGCATCAGCTATTATTACAGAAG
AAGGTGGATTAACTTCTCATGCAGCTGTTGTCGGATTAAGTCTTGGAATTCCAGTAATTGTTGGGGTTGACAATGTATTT
GAAATTCTTCAAGATGGACAAGATATTACTGTAGATGGTAACCGTGGAGATATCTACGCTGGCCATGCAGGTGTATTGTA
A

Upstream 100 bases:

>100_bases
TCATTCTTCATGGTTATAAAGAACAATATAGATAAAAAGCGTCTGATAGATTAAAAATCAACTTTAAAGGAATATGCAGC
AATATCAAGGAGGATATATA

Downstream 100 bases:

>100_bases
TTTAAAAATAATAGTTAAGGTTCCTGCCATTTTAGGCTAGGAACCTTTTTCATTTCAATATAGGAATAACCATTTAGTAA
TTTTAGGATAATGCTATCAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MRNTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGDYAEHGARIENIRKAAKNKNKTVAILLDTKGPEIRTGSFKEG
RADLMQGNPVTIAMDEVEGTAEKFSVTYQGLINDVHEGSKILLDDGLIELEVKSIDKSKGEIETIALNSGEIKNKKGVNV
PNVSVNLPGITEKDTNDIIFGIEQGVDFIAASFVRRASDILEIKEVLDQHQAQYIKIIPKIENQEGVDNIDSILEVSDGL
MVARGDLGVEIPAEDVPLVQKKLIKQCNNAGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGTY
PVESVQTMSNIALKAESALDHKQMLEERSQYVDMTITEAISQSVTHTASNLNVDTIITPTESGHTARMISKYRPKASILA
VTFNEVVNRQLSLVWGVHAVLGKPATSTDEMLEIAVDKGLNTGICSRGSRVIITAGVPVGESGTTNLLKVHVIGDVIAKG
QGIGRRSAYGHAVFAKTNKEALEKVQDGDILVTYATEKEMMPAIEKASAIITEEGGLTSHAAVVGLSLGIPVIVGVDNVF
EILQDGQDITVDGNRGDIYAGHAGVL

Sequences:

>Translated_586_residues
MRNTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGDYAEHGARIENIRKAAKNKNKTVAILLDTKGPEIRTGSFKEG
RADLMQGNPVTIAMDEVEGTAEKFSVTYQGLINDVHEGSKILLDDGLIELEVKSIDKSKGEIETIALNSGEIKNKKGVNV
PNVSVNLPGITEKDTNDIIFGIEQGVDFIAASFVRRASDILEIKEVLDQHQAQYIKIIPKIENQEGVDNIDSILEVSDGL
MVARGDLGVEIPAEDVPLVQKKLIKQCNNAGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGTY
PVESVQTMSNIALKAESALDHKQMLEERSQYVDMTITEAISQSVTHTASNLNVDTIITPTESGHTARMISKYRPKASILA
VTFNEVVNRQLSLVWGVHAVLGKPATSTDEMLEIAVDKGLNTGICSRGSRVIITAGVPVGESGTTNLLKVHVIGDVIAKG
QGIGRRSAYGHAVFAKTNKEALEKVQDGDILVTYATEKEMMPAIEKASAIITEEGGLTSHAAVVGLSLGIPVIVGVDNVF
EILQDGQDITVDGNRGDIYAGHAGVL
>Mature_586_residues
MRNTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGDYAEHGARIENIRKAAKNKNKTVAILLDTKGPEIRTGSFKEG
RADLMQGNPVTIAMDEVEGTAEKFSVTYQGLINDVHEGSKILLDDGLIELEVKSIDKSKGEIETIALNSGEIKNKKGVNV
PNVSVNLPGITEKDTNDIIFGIEQGVDFIAASFVRRASDILEIKEVLDQHQAQYIKIIPKIENQEGVDNIDSILEVSDGL
MVARGDLGVEIPAEDVPLVQKKLIKQCNNAGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGTY
PVESVQTMSNIALKAESALDHKQMLEERSQYVDMTITEAISQSVTHTASNLNVDTIITPTESGHTARMISKYRPKASILA
VTFNEVVNRQLSLVWGVHAVLGKPATSTDEMLEIAVDKGLNTGICSRGSRVIITAGVPVGESGTTNLLKVHVIGDVIAKG
QGIGRRSAYGHAVFAKTNKEALEKVQDGDILVTYATEKEMMPAIEKASAIITEEGGLTSHAAVVGLSLGIPVIVGVDNVF
EILQDGQDITVDGNRGDIYAGHAGVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=46.4212678936605, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=46.4212678936605, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=46.8302658486708, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=44.9897750511247, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=44.9897750511247, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=50, Blast_Score=259, Evalue=7e-69,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=50, Blast_Score=259, Evalue=7e-69,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=46.218487394958, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=46.218487394958, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=44.2396313364055, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1787965, Length=475, Percent_Identity=51.1578947368421, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=39.6659707724426, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1787994, Length=114, Percent_Identity=38.5964912280702, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=46.8041237113402, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=43.9271255060729, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=43.9271255060729, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=43.9271255060729, Blast_Score=356, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=43.9271255060729, Blast_Score=355, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6324923, Length=495, Percent_Identity=41.2121212121212, Blast_Score=336, Evalue=8e-93,
Organism=Saccharomyces cerevisiae, GI6319279, Length=497, Percent_Identity=41.6498993963783, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=43.7881873727088, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=43.7881873727088, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=45.7420924574209, Blast_Score=321, Evalue=7e-88,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=33.9468302658487, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24646914, Length=328, Percent_Identity=38.719512195122, Blast_Score=208, Evalue=7e-54,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62848; Mature: 62848

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGDYAEHGARIENIRKAAKNKNKT
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCHHHHHHHHHHCCCCCE
VAILLDTKGPEIRTGSFKEGRADLMQGNPVTIAMDEVEGTAEKFSVTYQGLINDVHEGSK
EEEEEECCCCCEECCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
ILLDDGLIELEVKSIDKSKGEIETIALNSGEIKNKKGVNVPNVSVNLPGITEKDTNDIIF
EEEECCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEE
GIEQGVDFIAASFVRRASDILEIKEVLDQHQAQYIKIIPKIENQEGVDNIDSILEVSDGL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCE
MVARGDLGVEIPAEDVPLVQKKLIKQCNNAGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGTYPVESVQTMSNIALKAESALDHKQMLEERSQYVDMTITEAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHH
SQSVTHTASNLNVDTIITPTESGHTARMISKYRPKASILAVTFNEVVNRQLSLVWGVHAV
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LGKPATSTDEMLEIAVDKGLNTGICSRGSRVIITAGVPVGESGTTNLLKVHVIGDVIAKG
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHHCC
QGIGRRSAYGHAVFAKTNKEALEKVQDGDILVTYATEKEMMPAIEKASAIITEEGGLTSH
CCCCCCCCCCCEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCCHH
AAVVGLSLGIPVIVGVDNVFEILQDGQDITVDGNRGDIYAGHAGVL
HHHEEHCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCEECCCCCCC
>Mature Secondary Structure
MRNTKIVCTIGPASESIEKLEQLIEAGMNVARLNFSHGDYAEHGARIENIRKAAKNKNKT
CCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCHHHHHHHHHHCCCCCE
VAILLDTKGPEIRTGSFKEGRADLMQGNPVTIAMDEVEGTAEKFSVTYQGLINDVHEGSK
EEEEEECCCCCEECCCCCCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCE
ILLDDGLIELEVKSIDKSKGEIETIALNSGEIKNKKGVNVPNVSVNLPGITEKDTNDIIF
EEEECCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEE
GIEQGVDFIAASFVRRASDILEIKEVLDQHQAQYIKIIPKIENQEGVDNIDSILEVSDGL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCE
MVARGDLGVEIPAEDVPLVQKKLIKQCNNAGKPVITATQMLDSMQRNPRPTRAEASDVAN
EEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHH
AIFDGTDAIMLSGETAAGTYPVESVQTMSNIALKAESALDHKQMLEERSQYVDMTITEAI
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHH
SQSVTHTASNLNVDTIITPTESGHTARMISKYRPKASILAVTFNEVVNRQLSLVWGVHAV
HHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
LGKPATSTDEMLEIAVDKGLNTGICSRGSRVIITAGVPVGESGTTNLLKVHVIGDVIAKG
HCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEHHHHHCC
QGIGRRSAYGHAVFAKTNKEALEKVQDGDILVTYATEKEMMPAIEKASAIITEEGGLTSH
CCCCCCCCCCCEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEECCCCCCHH
AAVVGLSLGIPVIVGVDNVFEILQDGQDITVDGNRGDIYAGHAGVL
HHHEEHCCCCEEEEEHHHHHHHHHCCCEEEEECCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]