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The map label for this gene is pfkA

Identifier: 23099627

GI number: 23099627

Start: 2207510

End: 2208469

Strand: Reverse

Name: pfkA

Synonym: OB2172

Alternate gene names: 23099627

Gene position: 2208469-2207510 (Counterclockwise)

Preceding gene: 23099628

Following gene: 23099626

Centisome position: 60.83

GC content: 37.19

Gene sequence:

>960_bases
ATGAAAAGAATAGGAGTATTAACAAGTGGCGGTGATTCTCCTGGTATGAACGCTGCTATCCGTGCGGTTGTTCGTAAGGC
GATTTATCATGACCTAGAAGTTTACGGTATTAAAAATGGTTATCAAGGATTAATGGATGGCAATATTGAAAAAATGACTT
TAGGGTCTGTAGGTGATATTATTCATCGAGGTGGAACAATACTGTTTTCTGCCAGAAGTGAAGAATTTAAAACAGATGAG
GGTCAATTTCGAGCTATTGAACAACTTAATAAGCATAATATAGATGGTTTAGTTGTTATTGGAGGAGATGGAAGCTTCCA
AGGTGCAAAAAAATTAACCGAGAAAGGTTTTCCATGTATAGGTGTACCGGGTACGATTGATAACGATATTGCAGGTACAG
ATTATACAATTGGTTTTGATACATCATTAAATACAATTATAGATGCTGTCGATAAAGTACGAGATACTGCTACATCTCAT
GAAAGAACTTACATTATTGAAGTAATGGGAAGAGATGCTGGTGACCTAGCACTATGGGCTGGGTTAGCTGTTGGCGCAGA
AAGTATATTGATACCGGAGGAAAAAGAAGACTTCTCCAGTATTGTTCAACGCTTAAAAAATGGACATGACCGTGGCAAAA
AACATAGTATCATCTTATTAGCTGAAGGTGTTGGTAGTGGCTTTGAATTCGGTAAGCGAATTGAAGAAGTTGCAGAACTT
GAGACTAGAGTTACAGTTTTAGGTCATATTCAGCGTGGTGGATCACCCACAGGGCAAGATCGTGTTCTTGCAAGCAGATT
GGGAGCCAGAGCTATTGAATTGTTGATTGATAATCAAGGTGGAAGAATGGTTGGAATTCAAAAAAACGTATTAGTGGATT
ATACATTTGATGAAGTGTTCAATGTTGAACATACAATCGATAAAGGAATGTATAAACTTTCGAAAGAATTATCAATCTAA

Upstream 100 bases:

>100_bases
TGTGGGGTTTATGATTGATATTTAAAGATTTTTTCTTTATCTTTAAATATATAGTTAGTACTTTGGCATTTAATGATTAT
TTAATTGGGGTGAACAATTC

Downstream 100 bases:

>100_bases
GTATGTGAGTCATTCTTCATGGTTATAAAGAACAATATAGATAAAAAGCGTCTGATAGATTAAAAATCAACTTTAAAGGA
ATATGCAGCAATATCAAGGA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDLEVYGIKNGYQGLMDGNIEKMTLGSVGDIIHRGGTILFSARSEEFKTDE
GQFRAIEQLNKHNIDGLVVIGGDGSFQGAKKLTEKGFPCIGVPGTIDNDIAGTDYTIGFDTSLNTIIDAVDKVRDTATSH
ERTYIIEVMGRDAGDLALWAGLAVGAESILIPEEKEDFSSIVQRLKNGHDRGKKHSIILLAEGVGSGFEFGKRIEEVAEL
ETRVTVLGHIQRGGSPTGQDRVLASRLGARAIELLIDNQGGRMVGIQKNVLVDYTFDEVFNVEHTIDKGMYKLSKELSI

Sequences:

>Translated_319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDLEVYGIKNGYQGLMDGNIEKMTLGSVGDIIHRGGTILFSARSEEFKTDE
GQFRAIEQLNKHNIDGLVVIGGDGSFQGAKKLTEKGFPCIGVPGTIDNDIAGTDYTIGFDTSLNTIIDAVDKVRDTATSH
ERTYIIEVMGRDAGDLALWAGLAVGAESILIPEEKEDFSSIVQRLKNGHDRGKKHSIILLAEGVGSGFEFGKRIEEVAEL
ETRVTVLGHIQRGGSPTGQDRVLASRLGARAIELLIDNQGGRMVGIQKNVLVDYTFDEVFNVEHTIDKGMYKLSKELSI
>Mature_319_residues
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDLEVYGIKNGYQGLMDGNIEKMTLGSVGDIIHRGGTILFSARSEEFKTDE
GQFRAIEQLNKHNIDGLVVIGGDGSFQGAKKLTEKGFPCIGVPGTIDNDIAGTDYTIGFDTSLNTIIDAVDKVRDTATSH
ERTYIIEVMGRDAGDLALWAGLAVGAESILIPEEKEDFSSIVQRLKNGHDRGKKHSIILLAEGVGSGFEFGKRIEEVAEL
ETRVTVLGHIQRGGSPTGQDRVLASRLGARAIELLIDNQGGRMVGIQKNVLVDYTFDEVFNVEHTIDKGMYKLSKELSI

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=347, Percent_Identity=40.0576368876081, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI266453619, Length=349, Percent_Identity=39.8280802292264, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI266453768, Length=349, Percent_Identity=39.8280802292264, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI266453748, Length=349, Percent_Identity=39.8280802292264, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI4505749, Length=349, Percent_Identity=39.8280802292264, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI48762920, Length=350, Percent_Identity=39.1428571428571, Blast_Score=216, Evalue=3e-56,
Organism=Escherichia coli, GI1790350, Length=318, Percent_Identity=52.2012578616352, Blast_Score=314, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI25147584, Length=347, Percent_Identity=38.0403458213257, Blast_Score=214, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17558788, Length=303, Percent_Identity=37.953795379538, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI72003601, Length=289, Percent_Identity=33.2179930795848, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321679, Length=332, Percent_Identity=37.0481927710843, Blast_Score=198, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6323861, Length=332, Percent_Identity=34.6385542168675, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=42.7652733118971, Blast_Score=227, Evalue=8e-60,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=42.7652733118971, Blast_Score=227, Evalue=9e-60,
Organism=Drosophila melanogaster, GI28573326, Length=311, Percent_Identity=41.8006430868167, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_OCEIH (Q8EPD6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693093.1
- ProteinModelPortal:   Q8EPD6
- SMR:   Q8EPD6
- GeneID:   1015559
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2172
- NMPDR:   fig|221109.1.peg.2170
- HOGENOM:   HBG405044
- OMA:   EADYDMN
- ProtClustDB:   PRK03202
- BioCyc:   OIHE221109:OB2172-MONOMER
- BRENDA:   2.7.1.11
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34577; Mature: 34577

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 243-243 BINDING 249-249 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDLEVYGIKNGYQGLMDGNIEKMTLGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEECCCHHHH
IHRGGTILFSARSEEFKTDEGQFRAIEQLNKHNIDGLVVIGGDGSFQGAKKLTEKGFPCI
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GVPGTIDNDIAGTDYTIGFDTSLNTIIDAVDKVRDTATSHERTYIIEVMGRDAGDLALWA
ECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
GLAVGAESILIPEEKEDFSSIVQRLKNGHDRGKKHSIILLAEGVGSGFEFGKRIEEVAEL
HHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ETRVTVLGHIQRGGSPTGQDRVLASRLGARAIELLIDNQGGRMVGIQKNVLVDYTFDEVF
HHHHHHEEHHHCCCCCCCHHHHHHHHHHHHEEEEEEECCCCEEEEEECCEEEEEEHHHHH
NVEHTIDKGMYKLSKELSI
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGVLTSGGDSPGMNAAIRAVVRKAIYHDLEVYGIKNGYQGLMDGNIEKMTLGSVGDI
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEECCCHHHH
IHRGGTILFSARSEEFKTDEGQFRAIEQLNKHNIDGLVVIGGDGSFQGAKKLTEKGFPCI
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCEE
GVPGTIDNDIAGTDYTIGFDTSLNTIIDAVDKVRDTATSHERTYIIEVMGRDAGDLALWA
ECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHH
GLAVGAESILIPEEKEDFSSIVQRLKNGHDRGKKHSIILLAEGVGSGFEFGKRIEEVAEL
HHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ETRVTVLGHIQRGGSPTGQDRVLASRLGARAIELLIDNQGGRMVGIQKNVLVDYTFDEVF
HHHHHHEEHHHCCCCCCCHHHHHHHHHHHHEEEEEEECCCCEEEEEECCEEEEEEHHHHH
NVEHTIDKGMYKLSKELSI
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376