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The map label for this gene is pheT

Identifier: 23099585

GI number: 23099585

Start: 2164661

End: 2167096

Strand: Reverse

Name: pheT

Synonym: OB2130

Alternate gene names: 23099585

Gene position: 2167096-2164661 (Counterclockwise)

Preceding gene: 23099586

Following gene: 23099582

Centisome position: 59.69

GC content: 35.92

Gene sequence:

>2436_bases
ATGTTAGTATCTTATAATTGGTTAAAAAATTACGTGAACTTAGATGCTGTTACACCTGAAGAACTTGCAGCAAAGATAAC
AAAATCAGGAATTGAAGTAGAGGGCGTCGAATACATCGCGGAAAAAAACGAAAATATAGTAGTTGGCTATGTGGAGACTT
GTGAAAAACATCCTGATGCGGATAAATTGAATGTAACACAAGTGAGTGTCGGAGAAGAGACATTACAAATTGTATGTGGT
GCGCCAAATATAGCTAAAGGGCAAAAAGTTGCAGTAGCTAAACCAGGAGCGGTACTGCCGGGGAATATGAAAATAAAAAA
AGTAAAACTTCGCGGTGTGGAATCTAACGGAATGATTTGCTCGCTACAGGAACTTGGATTAGAAGAGAAGTATATACCAA
CAGATATTGCAGAAGGAATATTTGTATTCCCAGAAGATGAGGATATCGAAGTTGGATCAAAAGTAGATGAGTGGTTGAAT
TTAACAGATGCAGTTTTAGACTTAGACGTCTTAGCAAATCGCCCTGATGCACTTAGTATGCTAGGAGTAGCTTATGAAGT
GGCAGCTGTTTTAGATCAACCGATTGAATTGCCAACTAAAAATATCGATTATATTGATGAGAAAGTATCTGATCAGATAG
AAATAAATATTGAATCCAAAGATTTAAATCCGTACTACGCAGCATTCATGGTTAAAGATGTAGTGATTAAACCTTCGCCT
TTATGGATGAGAAATTACTTAATGGCTTCAGGAATTCGACCAATTAATAATATCGTTGATATTACGAATTATGTTCTTTT
AGAGTATGGTCAACCATTGCATGCATTTGATTTCGATCGTTTTGCTTCAAATCAAATTGTTATTCGCAGAGGGGTTGAAG
GGGAAGTAATCCAAACATTGGATGAACAAGAAAGAAAACTAACCGAAGATAATCTTGTAATTACTAATGGAAGTGAGCCT
GTTGCCTTAGCAGGGGTAATGGGAGGAGCAAATAGTGAAGTTACGGATAAGACGGTTAACGTTTTATTAGAAGCTGCATA
TTTCGAAGCTACAGCAGTAAGACATACGGTGAGGCAAACTGGGCTGCGAAGTGAATCAAGTACCCGGTTTGAAAAAGGTA
TTGATCCTAATCGAATCGTTGAAGCGGGGTTAAGAGCATGTCAATTAATGCAGAGATATGCTGGTGGCAAAGTACTACAG
GGATTTGCCGAAGAAAATCATCTTGATACAAAATCAACAAAAGAAGTGAAAGTTCCGCTCACCCAAGTAAATCAAAGACT
AGGCATGGAATTAACAGAAAAGGATGTGCAGTCTGTCTTGAATCGATTAAAATTTGATTTTGATCAAGATGGCCATACAT
TTATTGTTCATGTGCCAACTCGTCGTAGAGATATTACGATTTTTGAGGATATGTTAGAAGAAATTATCCGTATTCATGGA
TATGATCATTTACCATATACAATTCCACAAGGTTGGGTGAAATCTGGCGGCCTAACAGATAAGCAACTACTGCAACGGAA
AATAAGAGAGTTTATGATGAGCAGTGGAGCAATGGAAACATTGACATATTCTTTAACTAATGAGCAAAATGTAAAGCGAT
TAGTTACGCCTAATATTGATTTAGAAAATAGTTATCCAATTTCATTAGCTATGCCAATGAGTGAGGATCATAAATATTTA
CGTCTAAGTATTGTGCCAGAATTGCTACGTATATTACAGCATAACATTGCTCGTAAGCAAACAGATTTAAATTACTTTGA
AATAGGAAAAGTATTTATTTCAGAAGAACAAGAATTGACGCATCAACCAACTGAAAAATTGCACTTAGCTGGGGCATTTA
CAGGATTATGGCATAATCATCCTTGGCAGCAAGATAAAAAGCAGGTTGACTTCTTTGTGGTTAAAGGAATTATTGAAGGC
TTATTCAACTATTTGCAGTTAAATATTTCCTTTAAACAGGTTAAAATGAAGGATATGCATCCAGGTCGATGTGCTGAACT
TAATATTGATGGAGAAAATATAGGATTTATGGGTCAATTACATCCAAAAACAGCAAATGTTTTTGACTTACCGGAGACAT
ATGTTTTTGAGATAGACTTAGAGTCTGTATTTGAAAAATATATAAATGTGCCATCCTTTACAGATATTCCAAGGTATCCA
TCCGTATCTCGTGATATTGCATTTATTCTTGATACTGAAGTATTCTCAGGAGAGGTACAACAGTATATACAAGAAATTGG
AGCACCATTAGTAAAGGAAGTTTCTATCTTTGATGTATATCAAGGGGAACACCTGGAAGATGGGAAAAAATCTGTCGCCT
ATCGTTTAATGTATCAAGATCCTGCAAAAACACTGCAAGATGACGAGGTAGAAAGTTCTTATCAACAAATTGTAGAAGCT
GTAAATAAGAAATTTGGTTCTTACGTTCGATCTTAA

Upstream 100 bases:

>100_bases
ATATGGCGTAAATGATATTCGTCATTTTTATACCAATGATATTCGATTCTTAACCCAATTTCACCAAGCATAAACGGACA
GAGATAAAAGGAGGAAGACC

Downstream 100 bases:

>100_bases
TGTAGAGATTTAATCAATTTCTCAATTAATATTTATGAAATCCGATGATTCGAACATTAATGTATGTTATCAAAAAAGGG
TAAAGTTAGCATCGATAACT

Product: phenylalanyl-tRNA synthetase beta subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MLVSYNWLKNYVNLDAVTPEELAAKITKSGIEVEGVEYIAEKNENIVVGYVETCEKHPDADKLNVTQVSVGEETLQIVCG
APNIAKGQKVAVAKPGAVLPGNMKIKKVKLRGVESNGMICSLQELGLEEKYIPTDIAEGIFVFPEDEDIEVGSKVDEWLN
LTDAVLDLDVLANRPDALSMLGVAYEVAAVLDQPIELPTKNIDYIDEKVSDQIEINIESKDLNPYYAAFMVKDVVIKPSP
LWMRNYLMASGIRPINNIVDITNYVLLEYGQPLHAFDFDRFASNQIVIRRGVEGEVIQTLDEQERKLTEDNLVITNGSEP
VALAGVMGGANSEVTDKTVNVLLEAAYFEATAVRHTVRQTGLRSESSTRFEKGIDPNRIVEAGLRACQLMQRYAGGKVLQ
GFAEENHLDTKSTKEVKVPLTQVNQRLGMELTEKDVQSVLNRLKFDFDQDGHTFIVHVPTRRRDITIFEDMLEEIIRIHG
YDHLPYTIPQGWVKSGGLTDKQLLQRKIREFMMSSGAMETLTYSLTNEQNVKRLVTPNIDLENSYPISLAMPMSEDHKYL
RLSIVPELLRILQHNIARKQTDLNYFEIGKVFISEEQELTHQPTEKLHLAGAFTGLWHNHPWQQDKKQVDFFVVKGIIEG
LFNYLQLNISFKQVKMKDMHPGRCAELNIDGENIGFMGQLHPKTANVFDLPETYVFEIDLESVFEKYINVPSFTDIPRYP
SVSRDIAFILDTEVFSGEVQQYIQEIGAPLVKEVSIFDVYQGEHLEDGKKSVAYRLMYQDPAKTLQDDEVESSYQQIVEA
VNKKFGSYVRS

Sequences:

>Translated_811_residues
MLVSYNWLKNYVNLDAVTPEELAAKITKSGIEVEGVEYIAEKNENIVVGYVETCEKHPDADKLNVTQVSVGEETLQIVCG
APNIAKGQKVAVAKPGAVLPGNMKIKKVKLRGVESNGMICSLQELGLEEKYIPTDIAEGIFVFPEDEDIEVGSKVDEWLN
LTDAVLDLDVLANRPDALSMLGVAYEVAAVLDQPIELPTKNIDYIDEKVSDQIEINIESKDLNPYYAAFMVKDVVIKPSP
LWMRNYLMASGIRPINNIVDITNYVLLEYGQPLHAFDFDRFASNQIVIRRGVEGEVIQTLDEQERKLTEDNLVITNGSEP
VALAGVMGGANSEVTDKTVNVLLEAAYFEATAVRHTVRQTGLRSESSTRFEKGIDPNRIVEAGLRACQLMQRYAGGKVLQ
GFAEENHLDTKSTKEVKVPLTQVNQRLGMELTEKDVQSVLNRLKFDFDQDGHTFIVHVPTRRRDITIFEDMLEEIIRIHG
YDHLPYTIPQGWVKSGGLTDKQLLQRKIREFMMSSGAMETLTYSLTNEQNVKRLVTPNIDLENSYPISLAMPMSEDHKYL
RLSIVPELLRILQHNIARKQTDLNYFEIGKVFISEEQELTHQPTEKLHLAGAFTGLWHNHPWQQDKKQVDFFVVKGIIEG
LFNYLQLNISFKQVKMKDMHPGRCAELNIDGENIGFMGQLHPKTANVFDLPETYVFEIDLESVFEKYINVPSFTDIPRYP
SVSRDIAFILDTEVFSGEVQQYIQEIGAPLVKEVSIFDVYQGEHLEDGKKSVAYRLMYQDPAKTLQDDEVESSYQQIVEA
VNKKFGSYVRS
>Mature_811_residues
MLVSYNWLKNYVNLDAVTPEELAAKITKSGIEVEGVEYIAEKNENIVVGYVETCEKHPDADKLNVTQVSVGEETLQIVCG
APNIAKGQKVAVAKPGAVLPGNMKIKKVKLRGVESNGMICSLQELGLEEKYIPTDIAEGIFVFPEDEDIEVGSKVDEWLN
LTDAVLDLDVLANRPDALSMLGVAYEVAAVLDQPIELPTKNIDYIDEKVSDQIEINIESKDLNPYYAAFMVKDVVIKPSP
LWMRNYLMASGIRPINNIVDITNYVLLEYGQPLHAFDFDRFASNQIVIRRGVEGEVIQTLDEQERKLTEDNLVITNGSEP
VALAGVMGGANSEVTDKTVNVLLEAAYFEATAVRHTVRQTGLRSESSTRFEKGIDPNRIVEAGLRACQLMQRYAGGKVLQ
GFAEENHLDTKSTKEVKVPLTQVNQRLGMELTEKDVQSVLNRLKFDFDQDGHTFIVHVPTRRRDITIFEDMLEEIIRIHG
YDHLPYTIPQGWVKSGGLTDKQLLQRKIREFMMSSGAMETLTYSLTNEQNVKRLVTPNIDLENSYPISLAMPMSEDHKYL
RLSIVPELLRILQHNIARKQTDLNYFEIGKVFISEEQELTHQPTEKLHLAGAFTGLWHNHPWQQDKKQVDFFVVKGIIEG
LFNYLQLNISFKQVKMKDMHPGRCAELNIDGENIGFMGQLHPKTANVFDLPETYVFEIDLESVFEKYINVPSFTDIPRYP
SVSRDIAFILDTEVFSGEVQQYIQEIGAPLVKEVSIFDVYQGEHLEDGKKSVAYRLMYQDPAKTLQDDEVESSYQQIVEA
VNKKFGSYVRS

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=277, Percent_Identity=21.6606498194946, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1788006, Length=818, Percent_Identity=34.718826405868, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21355211, Length=380, Percent_Identity=21.8421052631579, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_OCEIH (Q8EPH5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693051.1
- ProteinModelPortal:   Q8EPH5
- SMR:   Q8EPH5
- GeneID:   1015641
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2130
- NMPDR:   fig|221109.1.peg.2128
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- BioCyc:   OIHE221109:OB2130-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 91729; Mature: 91729

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSYNWLKNYVNLDAVTPEELAAKITKSGIEVEGVEYIAEKNENIVVGYVETCEKHPDA
CEEECHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEHHHHCCCCCC
DKLNVTQVSVGEETLQIVCGAPNIAKGQKVAVAKPGAVLPGNMKIKKVKLRGVESNGMIC
CCEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCEECCCCCEEEEEEEEEECCCCCEE
SLQELGLEEKYIPTDIAEGIFVFPEDEDIEVGSKVDEWLNLTDAVLDLDVLANRPDALSM
EHHHCCCCCCCCCHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGVAYEVAAVLDQPIELPTKNIDYIDEKVSDQIEINIESKDLNPYYAAFMVKDVVIKPSP
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHEECCCC
LWMRNYLMASGIRPINNIVDITNYVLLEYGQPLHAFDFDRFASNQIVIRRGVEGEVIQTL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHH
DEQERKLTEDNLVITNGSEPVALAGVMGGANSEVTDKTVNVLLEAAYFEATAVRHTVRQT
HHHHHHCCCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRSESSTRFEKGIDPNRIVEAGLRACQLMQRYAGGKVLQGFAEENHLDTKSTKEVKVPL
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCH
TQVNQRLGMELTEKDVQSVLNRLKFDFDQDGHTFIVHVPTRRRDITIFEDMLEEIIRIHG
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
YDHLPYTIPQGWVKSGGLTDKQLLQRKIREFMMSSGAMETLTYSLTNEQNVKRLVTPNID
CCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCEEECCCCC
LENSYPISLAMPMSEDHKYLRLSIVPELLRILQHNIARKQTDLNYFEIGKVFISEEQELT
CCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCHHHH
HQPTEKLHLAGAFTGLWHNHPWQQDKKQVDFFVVKGIIEGLFNYLQLNISFKQVKMKDMH
CCCHHHEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHEEEECCCC
PGRCAELNIDGENIGFMGQLHPKTANVFDLPETYVFEIDLESVFEKYINVPSFTDIPRYP
CCCEEEEEECCCCCCEEEEECCCCCCEEECCCEEEEEEEHHHHHHHHCCCCCCCCCCCCC
SVSRDIAFILDTEVFSGEVQQYIQEIGAPLVKEVSIFDVYQGEHLEDGKKSVAYRLMYQD
CCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCHHHHEEEEEECC
PAKTLQDDEVESSYQQIVEAVNKKFGSYVRS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLVSYNWLKNYVNLDAVTPEELAAKITKSGIEVEGVEYIAEKNENIVVGYVETCEKHPDA
CEEECHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEHHHHCCCCCC
DKLNVTQVSVGEETLQIVCGAPNIAKGQKVAVAKPGAVLPGNMKIKKVKLRGVESNGMIC
CCEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCEECCCCCEEEEEEEEEECCCCCEE
SLQELGLEEKYIPTDIAEGIFVFPEDEDIEVGSKVDEWLNLTDAVLDLDVLANRPDALSM
EHHHCCCCCCCCCHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
LGVAYEVAAVLDQPIELPTKNIDYIDEKVSDQIEINIESKDLNPYYAAFMVKDVVIKPSP
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHEECCCC
LWMRNYLMASGIRPINNIVDITNYVLLEYGQPLHAFDFDRFASNQIVIRRGVEGEVIQTL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCEEEEECCCCHHHHHH
DEQERKLTEDNLVITNGSEPVALAGVMGGANSEVTDKTVNVLLEAAYFEATAVRHTVRQT
HHHHHHCCCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLRSESSTRFEKGIDPNRIVEAGLRACQLMQRYAGGKVLQGFAEENHLDTKSTKEVKVPL
CCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEECCH
TQVNQRLGMELTEKDVQSVLNRLKFDFDQDGHTFIVHVPTRRRDITIFEDMLEEIIRIHG
HHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCC
YDHLPYTIPQGWVKSGGLTDKQLLQRKIREFMMSSGAMETLTYSLTNEQNVKRLVTPNID
CCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCEEECCCCC
LENSYPISLAMPMSEDHKYLRLSIVPELLRILQHNIARKQTDLNYFEIGKVFISEEQELT
CCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCHHHH
HQPTEKLHLAGAFTGLWHNHPWQQDKKQVDFFVVKGIIEGLFNYLQLNISFKQVKMKDMH
CCCHHHEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCHHEEEECCCC
PGRCAELNIDGENIGFMGQLHPKTANVFDLPETYVFEIDLESVFEKYINVPSFTDIPRYP
CCCEEEEEECCCCCCEEEEECCCCCCEEECCCEEEEEEEHHHHHHHHCCCCCCCCCCCCC
SVSRDIAFILDTEVFSGEVQQYIQEIGAPLVKEVSIFDVYQGEHLEDGKKSVAYRLMYQD
CCCCCEEEEEECHHHHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCCHHHHEEEEEECC
PAKTLQDDEVESSYQQIVEAVNKKFGSYVRS
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376