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The map label for this gene is pheS

Identifier: 23099586

GI number: 23099586

Start: 2167124

End: 2168158

Strand: Reverse

Name: pheS

Synonym: OB2131

Alternate gene names: 23099586

Gene position: 2168158-2167124 (Counterclockwise)

Preceding gene: 23099587

Following gene: 23099585

Centisome position: 59.72

GC content: 37.97

Gene sequence:

>1035_bases
ATGAAAGAAGAATTACACGCCATCCGTGATGAGGCAATTGCTTCTGTTGAAGAAGCAAAAGATATGAAATCATTACAGGA
TCTAAAAGTGAAATATTTAGGGAAGAAAGGTTCGTTAACGAGTGTTCTAAGAGGAATGGGTAAATTATCCAAGGAAGAAC
GCCCTGTCATTGGTGAAATAGCCAATCAAGTTCGTGAACAAATTACCGCTGCACTAGATCAAAAAACAGAAAAAATGGAA
GAAGAAGCATTAAATGAGAAATTAGAGAAAGAAACGATTGACGTTACTTTACCTGGTAATCCAGTACACATAGGCGGTAA
GCATTTATTAACCCGTGTTGTTGAAGAAATAGAGGATTTATTTATAGGTATGGGATATGAAGTGAAGGAAGGTCCCGAAG
TGGAAACAGATTACTTTAACTTTGAAGCATTGAACCTCCCAAAAAACCATCCAGCAAGAGATATGCAGGATACATTTTTT
ATAACAAATGAATTGTTACTTCGCACACAAACTTCACCAGTACAGGCACGTACGATGCAGGCTTACAATGGAGAGAAGCC
AATTAAAATGATATGTCCTGGTAAGGTTTATCGACGAGATACGGACGATGCGACGCACTCCCATCAATTTAGACAAATAG
AAGGGTTAGTTATTGATAAAGATATTACACTAAGTGATTTAAAAGGAACGTTAGACATCTTTGCAAAAAAAATGTTTGGA
GAAGATCGTGAAATTCGTCTACGTCCATCATTCTTCCCATTTACAGAGCCGTCAGTAGAGATGGATGTTTCCTGTAAAGT
ATGCAGTGGCCAAGGGTGCTCCGTATGTAAGCAGACTGGTTGGATTGAAATTTTAGGAGGAGGAATGGTACATCCTCGAG
TTTTAGAGATGAGTGGCTATGATCCAAAAGTTTATAGCGGATTTGCATTTGGAATGGGGCAAGAGCGTATCGCAATGCTT
AAATATGGCGTAAATGATATTCGTCATTTTTATACCAATGATATTCGATTCTTAACCCAATTTCACCAAGCATAA

Upstream 100 bases:

>100_bases
TAATCAACAAGGGTGGTACCGCGACCATAGAGCCTCGTCCCTTTCTGGGATAGAGGCTTTTTTGTTTTTCGTATTGATAA
ATATTAGGGAGGTATAAAGA

Downstream 100 bases:

>100_bases
ACGGACAGAGATAAAAGGAGGAAGACCATGTTAGTATCTTATAATTGGTTAAAAAATTACGTGAACTTAGATGCTGTTAC
ACCTGAAGAACTTGCAGCAA

Product: phenylalanyl-tRNA synthetase alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKEELHAIRDEAIASVEEAKDMKSLQDLKVKYLGKKGSLTSVLRGMGKLSKEERPVIGEIANQVREQITAALDQKTEKME
EEALNEKLEKETIDVTLPGNPVHIGGKHLLTRVVEEIEDLFIGMGYEVKEGPEVETDYFNFEALNLPKNHPARDMQDTFF
ITNELLLRTQTSPVQARTMQAYNGEKPIKMICPGKVYRRDTDDATHSHQFRQIEGLVIDKDITLSDLKGTLDIFAKKMFG
EDREIRLRPSFFPFTEPSVEMDVSCKVCSGQGCSVCKQTGWIEILGGGMVHPRVLEMSGYDPKVYSGFAFGMGQERIAML
KYGVNDIRHFYTNDIRFLTQFHQA

Sequences:

>Translated_344_residues
MKEELHAIRDEAIASVEEAKDMKSLQDLKVKYLGKKGSLTSVLRGMGKLSKEERPVIGEIANQVREQITAALDQKTEKME
EEALNEKLEKETIDVTLPGNPVHIGGKHLLTRVVEEIEDLFIGMGYEVKEGPEVETDYFNFEALNLPKNHPARDMQDTFF
ITNELLLRTQTSPVQARTMQAYNGEKPIKMICPGKVYRRDTDDATHSHQFRQIEGLVIDKDITLSDLKGTLDIFAKKMFG
EDREIRLRPSFFPFTEPSVEMDVSCKVCSGQGCSVCKQTGWIEILGGGMVHPRVLEMSGYDPKVYSGFAFGMGQERIAML
KYGVNDIRHFYTNDIRFLTQFHQA
>Mature_344_residues
MKEELHAIRDEAIASVEEAKDMKSLQDLKVKYLGKKGSLTSVLRGMGKLSKEERPVIGEIANQVREQITAALDQKTEKME
EEALNEKLEKETIDVTLPGNPVHIGGKHLLTRVVEEIEDLFIGMGYEVKEGPEVETDYFNFEALNLPKNHPARDMQDTFF
ITNELLLRTQTSPVQARTMQAYNGEKPIKMICPGKVYRRDTDDATHSHQFRQIEGLVIDKDITLSDLKGTLDIFAKKMFG
EDREIRLRPSFFPFTEPSVEMDVSCKVCSGQGCSVCKQTGWIEILGGGMVHPRVLEMSGYDPKVYSGFAFGMGQERIAML
KYGVNDIRHFYTNDIRFLTQFHQA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=274, Percent_Identity=30.2919708029197, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI5729820, Length=355, Percent_Identity=27.0422535211268, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1788007, Length=330, Percent_Identity=50, Blast_Score=341, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17508957, Length=273, Percent_Identity=31.1355311355311, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=273, Percent_Identity=31.1355311355311, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=251, Percent_Identity=34.6613545816733, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6321087, Length=272, Percent_Identity=30.8823529411765, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=282, Percent_Identity=28.3687943262411, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18858079, Length=297, Percent_Identity=30.976430976431, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137424, Length=296, Percent_Identity=27.027027027027, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_OCEIH (Q8EPH4)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693052.1
- ProteinModelPortal:   Q8EPH4
- SMR:   Q8EPH4
- GeneID:   1017697
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2131
- NMPDR:   fig|221109.1.peg.2129
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- BioCyc:   OIHE221109:OB2131-MONOMER
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39032; Mature: 39032

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEELHAIRDEAIASVEEAKDMKSLQDLKVKYLGKKGSLTSVLRGMGKLSKEERPVIGEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
ANQVREQITAALDQKTEKMEEEALNEKLEKETIDVTLPGNPVHIGGKHLLTRVVEEIEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEECHHHHHHHHHHHHHHH
FIGMGYEVKEGPEVETDYFNFEALNLPKNHPARDMQDTFFITNELLLRTQTSPVQARTMQ
HHCCCCCCCCCCCCCCCEECEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHH
AYNGEKPIKMICPGKVYRRDTDDATHSHQFRQIEGLVIDKDITLSDLKGTLDIFAKKMFG
HHCCCCCEEEECCCHHCCCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHC
EDREIRLRPSFFPFTEPSVEMDVSCKVCSGQGCSVCKQTGWIEILGGGMVHPRVLEMSGY
CCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCEEEECCCCCCCEEEECCCC
DPKVYSGFAFGMGQERIAMLKYGVNDIRHFYTNDIRFLTQFHQA
CCHHHCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEELHAIRDEAIASVEEAKDMKSLQDLKVKYLGKKGSLTSVLRGMGKLSKEERPVIGEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHH
ANQVREQITAALDQKTEKMEEEALNEKLEKETIDVTLPGNPVHIGGKHLLTRVVEEIEDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEECHHHHHHHHHHHHHHH
FIGMGYEVKEGPEVETDYFNFEALNLPKNHPARDMQDTFFITNELLLRTQTSPVQARTMQ
HHCCCCCCCCCCCCCCCEECEEEECCCCCCCCCCCHHHHHHHHHHHEEECCCCHHHHHHH
AYNGEKPIKMICPGKVYRRDTDDATHSHQFRQIEGLVIDKDITLSDLKGTLDIFAKKMFG
HHCCCCCEEEECCCHHCCCCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHC
EDREIRLRPSFFPFTEPSVEMDVSCKVCSGQGCSVCKQTGWIEILGGGMVHPRVLEMSGY
CCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCHHHHHCCCEEEECCCCCCCEEEECCCC
DPKVYSGFAFGMGQERIAMLKYGVNDIRHFYTNDIRFLTQFHQA
CCHHHCCEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376