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The map label for this gene is ruvB

Identifier: 23099491

GI number: 23099491

Start: 2064694

End: 2065698

Strand: Reverse

Name: ruvB

Synonym: OB2036

Alternate gene names: 23099491

Gene position: 2065698-2064694 (Counterclockwise)

Preceding gene: 23099492

Following gene: 23099490

Centisome position: 56.9

GC content: 39.1

Gene sequence:

>1005_bases
ATGGATGATCGAATGATAGACGGAGAGCTTCAAGATCAAGATGTAGAGATAGAACTTAGTCTACGTCCTTCGACGCTGTC
CCAATATATTGGACAGGATAAAGTGAAAGAAAACCTGACTATCTTTATTCAAGCAGCAAAAATGCGTGAAGAACCGTTAG
ATCATGTGTTATTATATGGTCCTCCAGGATTAGGAAAAACAACCCTAGCATCAATCATATCTTATGAGATGGGGGTTCAA
TTTCGATCTACTTCTGGTCCCGCTATTGAAAGAGCTGGAGACTTAGCAGCGATATTATCTTCCCTTGAACCAGGAGATGT
TTTATTTATTGATGAAGTGCACCGGCTTCCTAGATCGGTAGAAGAAGTGCTCTATGCTGCTATGGAAGATTTCTTTATTG
ATATTGTTATTGGTACAGGCCCAAGTGCACGGTCGGTAAGAATTGATTTACCTCCGTTTACGTTAGTAGGAGCTACGACA
AGAGCTGGTTTATTAAGTGCCCCCTTAAGAGATAGATTTGGAGTACTTAGTCGTCTTGAATATTATGAGATAAAGGATTT
ATGCAATATTGTGGAACGTACTGCTGATATATTTAATATGCCAATTTCTTCAGAAGCAGCCATTGAAGTGGCTAGACGAT
CAAGGGGAACTCCGCGAATTGCTAATCGGCTTTTAAAACGTGTTAGAGACATATCTCAAGTAAAAGGAGAAGAGGAAATA
AGTCTAGAATCAACAAAGCAAGCATTAGAAATGCTTCAAGTAGACGATGCAGGATTAGACCACGTTGATCACAAGTTGCT
TACAGGAATTATTGAAGGATTTAGTGGTGGACCAGTAGGTTTAGATACAATCGCTGCTACGATTGGAGAGGAATCTCAAA
CAATTGAAGAAGTTTATGAACCCTTCTTATTACAGCTTGGTTTTATTCAACGTACACCTAGGGGAAGAGTCATTACATCA
AAAGCGTATGATCATTTAGGAATAAAAAGGACTGGTGAAGATTAA

Upstream 100 bases:

>100_bases
GTACTACCTCATTTAAAGAAGGATAATGATGCGTTAAGTGTAGATGAAGCTATTCGCAAAGCATTAGCATTATTAGCAAA
GTAGGAAAGGGGGAGAATAA

Downstream 100 bases:

>100_bases
ACATGGGTAAAATGTTTATTATCATTGGAGTTGTTTTTATCATAATTGGTATTCTGTGGACGTTCATTGGCAGATTGCCC
GGGGATATTACTATAAATAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MDDRMIDGELQDQDVEIELSLRPSTLSQYIGQDKVKENLTIFIQAAKMREEPLDHVLLYGPPGLGKTTLASIISYEMGVQ
FRSTSGPAIERAGDLAAILSSLEPGDVLFIDEVHRLPRSVEEVLYAAMEDFFIDIVIGTGPSARSVRIDLPPFTLVGATT
RAGLLSAPLRDRFGVLSRLEYYEIKDLCNIVERTADIFNMPISSEAAIEVARRSRGTPRIANRLLKRVRDISQVKGEEEI
SLESTKQALEMLQVDDAGLDHVDHKLLTGIIEGFSGGPVGLDTIAATIGEESQTIEEVYEPFLLQLGFIQRTPRGRVITS
KAYDHLGIKRTGED

Sequences:

>Translated_334_residues
MDDRMIDGELQDQDVEIELSLRPSTLSQYIGQDKVKENLTIFIQAAKMREEPLDHVLLYGPPGLGKTTLASIISYEMGVQ
FRSTSGPAIERAGDLAAILSSLEPGDVLFIDEVHRLPRSVEEVLYAAMEDFFIDIVIGTGPSARSVRIDLPPFTLVGATT
RAGLLSAPLRDRFGVLSRLEYYEIKDLCNIVERTADIFNMPISSEAAIEVARRSRGTPRIANRLLKRVRDISQVKGEEEI
SLESTKQALEMLQVDDAGLDHVDHKLLTGIIEGFSGGPVGLDTIAATIGEESQTIEEVYEPFLLQLGFIQRTPRGRVITS
KAYDHLGIKRTGED
>Mature_334_residues
MDDRMIDGELQDQDVEIELSLRPSTLSQYIGQDKVKENLTIFIQAAKMREEPLDHVLLYGPPGLGKTTLASIISYEMGVQ
FRSTSGPAIERAGDLAAILSSLEPGDVLFIDEVHRLPRSVEEVLYAAMEDFFIDIVIGTGPSARSVRIDLPPFTLVGATT
RAGLLSAPLRDRFGVLSRLEYYEIKDLCNIVERTADIFNMPISSEAAIEVARRSRGTPRIANRLLKRVRDISQVKGEEEI
SLESTKQALEMLQVDDAGLDHVDHKLLTGIIEGFSGGPVGLDTIAATIGEESQTIEEVYEPFLLQLGFIQRTPRGRVITS
KAYDHLGIKRTGED

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=327, Percent_Identity=59.3272171253823, Blast_Score=405, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_OCEIH (Q8EPQ6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692957.1
- ProteinModelPortal:   Q8EPQ6
- SMR:   Q8EPQ6
- GeneID:   1018526
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2036
- NMPDR:   fig|221109.1.peg.2034
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   OIHE221109:OB2036-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 36911; Mature: 36911

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRMIDGELQDQDVEIELSLRPSTLSQYIGQDKVKENLTIFIQAAKMREEPLDHVLLYG
CCCCCCCCCCCCCCEEEEEEECHHHHHHHHCHHHHHCCEEEEEEEHHHHCCCCCEEEEEC
PPGLGKTTLASIISYEMGVQFRSTSGPAIERAGDLAAILSSLEPGDVLFIDEVHRLPRSV
CCCCCHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYAAMEDFFIDIVIGTGPSARSVRIDLPPFTLVGATTRAGLLSAPLRDRFGVLSRLE
HHHHHHHHHHHEEEEEEECCCCCCEEEEECCCEEEECCCHHHHHHHCCHHHHHHHHHHHH
YYEIKDLCNIVERTADIFNMPISSEAAIEVARRSRGTPRIANRLLKRVRDISQVKGEEEI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
SLESTKQALEMLQVDDAGLDHVDHKLLTGIIEGFSGGPVGLDTIAATIGEESQTIEEVYE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
PFLLQLGFIQRTPRGRVITSKAYDHLGIKRTGED
HHHHHHHHHHCCCCCCEEECCHHHHCCCEECCCC
>Mature Secondary Structure
MDDRMIDGELQDQDVEIELSLRPSTLSQYIGQDKVKENLTIFIQAAKMREEPLDHVLLYG
CCCCCCCCCCCCCCEEEEEEECHHHHHHHHCHHHHHCCEEEEEEEHHHHCCCCCEEEEEC
PPGLGKTTLASIISYEMGVQFRSTSGPAIERAGDLAAILSSLEPGDVLFIDEVHRLPRSV
CCCCCHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHH
EEVLYAAMEDFFIDIVIGTGPSARSVRIDLPPFTLVGATTRAGLLSAPLRDRFGVLSRLE
HHHHHHHHHHHEEEEEEECCCCCCEEEEECCCEEEECCCHHHHHHHCCHHHHHHHHHHHH
YYEIKDLCNIVERTADIFNMPISSEAAIEVARRSRGTPRIANRLLKRVRDISQVKGEEEI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
SLESTKQALEMLQVDDAGLDHVDHKLLTGIIEGFSGGPVGLDTIAATIGEESQTIEEVYE
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHH
PFLLQLGFIQRTPRGRVITSKAYDHLGIKRTGED
HHHHHHHHHHCCCCCCEEECCHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376