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The map label for this gene is ruvA

Identifier: 23099492

GI number: 23099492

Start: 2065715

End: 2066320

Strand: Reverse

Name: ruvA

Synonym: OB2037

Alternate gene names: 23099492

Gene position: 2066320-2065715 (Counterclockwise)

Preceding gene: 23099493

Following gene: 23099491

Centisome position: 56.92

GC content: 33.99

Gene sequence:

>606_bases
ATGATTGCATATATTAAAGGTACTTTGAATTCCTTAGGGGATGAATCGCTTATTGTAGATGTAGGTGGAATTGGTTATGA
AATAGTGTGCCCAAACCCTTTTGTTTTTCAAGATTTACTCAATCAGCAAATACATATACAAACCTATCATCATGTGCGAG
AGGATGCACAAATTCTTTTTGGTTTTCAAAATAGAGATGAGAAATACTTATTTACGAAGTTAATATCTGTTTCAGGAATT
GGTCCAAAGGGAGCTTTAGCTATTTTAGCAGGAGTGGATATATCAGGGTTTATTGCTGCAGTTGAAAATGAGGATGATAA
GTTCTTAACTAGTTTTCCTGGTGTTGGAAAAAAGACAGCAAGACAAATCATTCTTGATTTAAAAGGAAAGTTAACAAGTG
TATTCTCCATAACTGATGAACAGCAAAAGAGTAGTGTTAGTAATGTCAATAATAATGAAGTATATTCTGAAGCGATGGAA
GCACTGAAGGCACTAGGTTATACAGATAAAGAGGTTAAGCAAGTACTACCTCATTTAAAGAAGGATAATGATGCGTTAAG
TGTAGATGAAGCTATTCGCAAAGCATTAGCATTATTAGCAAAGTAG

Upstream 100 bases:

>100_bases
GTAATGGAAGATGGAGTCTACCTCATCGTAACATTCGAGATTTTAAATAAAATATATAGTTATGAAATTGATTTATGTAA
TTAAATAGGGGATGAAAATA

Downstream 100 bases:

>100_bases
GAAAGGGGGAGAATAAATGGATGATCGAATGATAGACGGAGAGCTTCAAGATCAAGATGTAGAGATAGAACTTAGTCTAC
GTCCTTCGACGCTGTCCCAA

Product: holliday junction DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIAYIKGTLNSLGDESLIVDVGGIGYEIVCPNPFVFQDLLNQQIHIQTYHHVREDAQILFGFQNRDEKYLFTKLISVSGI
GPKGALAILAGVDISGFIAAVENEDDKFLTSFPGVGKKTARQIILDLKGKLTSVFSITDEQQKSSVSNVNNNEVYSEAME
ALKALGYTDKEVKQVLPHLKKDNDALSVDEAIRKALALLAK

Sequences:

>Translated_201_residues
MIAYIKGTLNSLGDESLIVDVGGIGYEIVCPNPFVFQDLLNQQIHIQTYHHVREDAQILFGFQNRDEKYLFTKLISVSGI
GPKGALAILAGVDISGFIAAVENEDDKFLTSFPGVGKKTARQIILDLKGKLTSVFSITDEQQKSSVSNVNNNEVYSEAME
ALKALGYTDKEVKQVLPHLKKDNDALSVDEAIRKALALLAK
>Mature_201_residues
MIAYIKGTLNSLGDESLIVDVGGIGYEIVCPNPFVFQDLLNQQIHIQTYHHVREDAQILFGFQNRDEKYLFTKLISVSGI
GPKGALAILAGVDISGFIAAVENEDDKFLTSFPGVGKKTARQIILDLKGKLTSVFSITDEQQKSSVSNVNNNEVYSEAME
ALKALGYTDKEVKQVLPHLKKDNDALSVDEAIRKALALLAK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=204, Percent_Identity=34.8039215686275, Blast_Score=120, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_OCEIH (Q8EPQ5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692958.1
- ProteinModelPortal:   Q8EPQ5
- SMR:   Q8EPQ5
- GeneID:   1018527
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2037
- NMPDR:   fig|221109.1.peg.2035
- HOGENOM:   HBG635309
- OMA:   LFGFAQK
- BioCyc:   OIHE221109:OB2037-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 22042; Mature: 22042

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAYIKGTLNSLGDESLIVDVGGIGYEIVCPNPFVFQDLLNQQIHIQTYHHVREDAQILF
CEEEEECHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHCCCCEEEHHHHHHHHCHHEEE
GFQNRDEKYLFTKLISVSGIGPKGALAILAGVDISGFIAAVENEDDKFLTSFPGVGKKTA
EECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHEEEEEECCCCCEECCCCCCCHHHH
RQIILDLKGKLTSVFSITDEQQKSSVSNVNNNEVYSEAMEALKALGYTDKEVKQVLPHLK
HHHHHHHCCCHHHHEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
KDNDALSVDEAIRKALALLAK
CCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAYIKGTLNSLGDESLIVDVGGIGYEIVCPNPFVFQDLLNQQIHIQTYHHVREDAQILF
CEEEEECHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHCCCCEEEHHHHHHHHCHHEEE
GFQNRDEKYLFTKLISVSGIGPKGALAILAGVDISGFIAAVENEDDKFLTSFPGVGKKTA
EECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHEEEEEECCCCCEECCCCCCCHHHH
RQIILDLKGKLTSVFSITDEQQKSSVSNVNNNEVYSEAMEALKALGYTDKEVKQVLPHLK
HHHHHHHCCCHHHHEECCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
KDNDALSVDEAIRKALALLAK
CCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376