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The map label for this gene is aspS

Identifier: 23099474

GI number: 23099474

Start: 2044057

End: 2045832

Strand: Reverse

Name: aspS

Synonym: OB2019

Alternate gene names: 23099474

Gene position: 2045832-2044057 (Counterclockwise)

Preceding gene: 23099475

Following gene: 23099471

Centisome position: 56.35

GC content: 36.54

Gene sequence:

>1776_bases
ATGAGTGAAAGATTAATGGCGGGTAGGCTAAACGAAAAAAATATAGGTGAAAAAGTATTGCTTAAAGGTTGGGTTCAAAA
GCGTCGAGATCTTGGCGGGTTGATTTTTATTGATCTTCGTGATAAATCAGGACTCATACAAGTTGTCTTTAATCCTGACC
ATTCTAAAAAGGCTTTAGAAACAGCGGAAAATATTCGTTCAGAATATGTAATTGAAATCAATGGCACGGTAGTTGCTCGT
GATGAAGCAACCATCAATCCATCGATGAAAACGGGTAAAATTGAAGTTAATGCTTCTTCAGTCCAAATTTTAAATAAAGC
AAAAACACCACCGTTTACCATACAAGATGAAACAGATGTTTCAGAAGATGTCAGGCTTAAATATAGATATTTGGACTTGC
GTAGAAATTCATTACAAGAAACATTTCGATTGCGTCATCAAACAACCCAATCTATTCGAAATTACTTAAATGACAAAGAT
TTCTTAGAGATGGAGACACCAATTCTAACGAAGAGTACGCCAGAAGGTGCAAGAGATTATTTAGTTCCTAGTCGTGTACA
CCCGGGAGAATTTTATGCCTTACCTCAGTCTCCACAATTGTTTAAGCAATTAATTATGATGGGTGGTTTTGAGCGATATT
ATCAAATCGCAAGATGCTTCCGTGATGAGGACTTGCGTGCAGATAGGCAACCTGAATTCACACAAATCGATATAGAAACG
TCATTTTTAACTAGTGATGAAATTATGGATATGACAGAACATATGATGAAAAAAGTAATGAAAGAAGTAAAAGATATAGA
TATTTCTTTACCATTACCACGTATGCCATATCAAGAGGCAATGGATAGATATGGTTCTGATAAACCAGACACACGTTTCG
ATTTAGAATTAATTCATGTTTCTGATATCGTGGCTTCATCTGGATTTAAGGTATTTTCTCAAGCGGTAGATAACGGCGGG
AAGGTAGCCTTATTAAATATCAAAGGCAAAGCGGATAACTATTCTCGTAAAGATATTGATAAATTAACAGAATATGTAAA
AGTTTATGATGCTAAAGGGCTTGCATGGCTAAAAGCGGATGAAAGCGAATTGAAAGGTCCTATTGCTAAATTCTTATCTG
AAGAGGAAGTTGCAGGTATTCGTGATCGTGCAAATGTAGAGCAAGGAGACTTATTGTTATTTGTAGCGGATAAAACGAAT
GTTGTATACGATAGTCTTGGAGCTTTACGTCTTTACTTAGGTAAGGAATTAGGTTTAATAGATGAATCCAAATTCCATTT
CCTATGGGTTACAGATTGGCCATTATTAGAGTATGACGAAGGCTTAGGCAGATATTTTGCAGCACACCACCCATTTACAT
CTGCAATCGAAGAAGATTTAGATAAATTAGAAACTGATCCTGCGAGTGTACGTGCAAATGCTTATGACTTAGTGTTAAAT
GGTTTTGAACTTGGAGGAGGATCCATTCGTATTCATCAAAAGGAACAACAGGACCAAATGTTTAAAGTTCTAGGGTTCTC
AGAAGAAGAAGCACGTAGTCAATTTGGATTCTTATTAGATGCTTTAGAATATGGTGCACCTCCACATGGAGGTATAGCTC
TTGGATTGGATCGCATTATCATGTTGTTAGCAGGTAGATCTAACCTCCGTGATACCATTCTATTTCCAAAAACAGCTTCA
GCCTCTGATTTAATGACTGCAGCTCCAAGTGGTGTAAGTGATGATCAATTACAAGAATTATCTATTCAATTGCAATCAGG
GGAAAAGAACCAGTAA

Upstream 100 bases:

>100_bases
GAAGTCACTTTAAAGGAAATGTCTACTGGTGATCAAAGTGAGGTTTCCATGTCTCAACTGGTTGAAGTGCTTCAGGAAAA
GCTGAATGGAGGAAAAATAT

Downstream 100 bases:

>100_bases
AATATCGTACAACAAACCTATTGTTTACAAAAAATAAATATGCTAATATAAATACACAATAAAATTTAATTTACGTATCA
TCCTGATGTGTTCGTCTATT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MSERLMAGRLNEKNIGEKVLLKGWVQKRRDLGGLIFIDLRDKSGLIQVVFNPDHSKKALETAENIRSEYVIEINGTVVAR
DEATINPSMKTGKIEVNASSVQILNKAKTPPFTIQDETDVSEDVRLKYRYLDLRRNSLQETFRLRHQTTQSIRNYLNDKD
FLEMETPILTKSTPEGARDYLVPSRVHPGEFYALPQSPQLFKQLIMMGGFERYYQIARCFRDEDLRADRQPEFTQIDIET
SFLTSDEIMDMTEHMMKKVMKEVKDIDISLPLPRMPYQEAMDRYGSDKPDTRFDLELIHVSDIVASSGFKVFSQAVDNGG
KVALLNIKGKADNYSRKDIDKLTEYVKVYDAKGLAWLKADESELKGPIAKFLSEEEVAGIRDRANVEQGDLLLFVADKTN
VVYDSLGALRLYLGKELGLIDESKFHFLWVTDWPLLEYDEGLGRYFAAHHPFTSAIEEDLDKLETDPASVRANAYDLVLN
GFELGGGSIRIHQKEQQDQMFKVLGFSEEEARSQFGFLLDALEYGAPPHGGIALGLDRIIMLLAGRSNLRDTILFPKTAS
ASDLMTAAPSGVSDDQLQELSIQLQSGEKNQ

Sequences:

>Translated_591_residues
MSERLMAGRLNEKNIGEKVLLKGWVQKRRDLGGLIFIDLRDKSGLIQVVFNPDHSKKALETAENIRSEYVIEINGTVVAR
DEATINPSMKTGKIEVNASSVQILNKAKTPPFTIQDETDVSEDVRLKYRYLDLRRNSLQETFRLRHQTTQSIRNYLNDKD
FLEMETPILTKSTPEGARDYLVPSRVHPGEFYALPQSPQLFKQLIMMGGFERYYQIARCFRDEDLRADRQPEFTQIDIET
SFLTSDEIMDMTEHMMKKVMKEVKDIDISLPLPRMPYQEAMDRYGSDKPDTRFDLELIHVSDIVASSGFKVFSQAVDNGG
KVALLNIKGKADNYSRKDIDKLTEYVKVYDAKGLAWLKADESELKGPIAKFLSEEEVAGIRDRANVEQGDLLLFVADKTN
VVYDSLGALRLYLGKELGLIDESKFHFLWVTDWPLLEYDEGLGRYFAAHHPFTSAIEEDLDKLETDPASVRANAYDLVLN
GFELGGGSIRIHQKEQQDQMFKVLGFSEEEARSQFGFLLDALEYGAPPHGGIALGLDRIIMLLAGRSNLRDTILFPKTAS
ASDLMTAAPSGVSDDQLQELSIQLQSGEKNQ
>Mature_590_residues
SERLMAGRLNEKNIGEKVLLKGWVQKRRDLGGLIFIDLRDKSGLIQVVFNPDHSKKALETAENIRSEYVIEINGTVVARD
EATINPSMKTGKIEVNASSVQILNKAKTPPFTIQDETDVSEDVRLKYRYLDLRRNSLQETFRLRHQTTQSIRNYLNDKDF
LEMETPILTKSTPEGARDYLVPSRVHPGEFYALPQSPQLFKQLIMMGGFERYYQIARCFRDEDLRADRQPEFTQIDIETS
FLTSDEIMDMTEHMMKKVMKEVKDIDISLPLPRMPYQEAMDRYGSDKPDTRFDLELIHVSDIVASSGFKVFSQAVDNGGK
VALLNIKGKADNYSRKDIDKLTEYVKVYDAKGLAWLKADESELKGPIAKFLSEEEVAGIRDRANVEQGDLLLFVADKTNV
VYDSLGALRLYLGKELGLIDESKFHFLWVTDWPLLEYDEGLGRYFAAHHPFTSAIEEDLDKLETDPASVRANAYDLVLNG
FELGGGSIRIHQKEQQDQMFKVLGFSEEEARSQFGFLLDALEYGAPPHGGIALGLDRIIMLLAGRSNLRDTILFPKTASA
SDLMTAAPSGVSDDQLQELSIQLQSGEKNQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=592, Percent_Identity=40.5405405405405, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI45439306, Length=266, Percent_Identity=27.4436090225564, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI5031815, Length=303, Percent_Identity=25.7425742574257, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194272210, Length=301, Percent_Identity=25.9136212624585, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226491502, Length=254, Percent_Identity=25.1968503937008, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=52.027027027027, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1789256, Length=289, Percent_Identity=25.2595155709343, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=25.5172413793103, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=596, Percent_Identity=36.0738255033557, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17551876, Length=282, Percent_Identity=28.0141843971631, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71984122, Length=290, Percent_Identity=25.1724137931034, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=258, Percent_Identity=21.7054263565891, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=258, Percent_Identity=22.093023255814, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=258, Percent_Identity=22.093023255814, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=640, Percent_Identity=32.65625, Blast_Score=286, Evalue=5e-78,
Organism=Saccharomyces cerevisiae, GI6323011, Length=251, Percent_Identity=29.4820717131474, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=262, Percent_Identity=25.9541984732824, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=178, Percent_Identity=28.6516853932584, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=176, Percent_Identity=26.1363636363636, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24584738, Length=590, Percent_Identity=38.8135593220339, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17136276, Length=265, Percent_Identity=27.1698113207547, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640849, Length=257, Percent_Identity=26.4591439688716, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24640851, Length=257, Percent_Identity=26.4591439688716, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19921528, Length=279, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_OCEIH (Q8EPS0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692940.1
- ProteinModelPortal:   Q8EPS0
- SMR:   Q8EPS0
- GeneID:   1018508
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2019
- NMPDR:   fig|221109.1.peg.2018
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- BioCyc:   OIHE221109:OB2019-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66934; Mature: 66803

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERLMAGRLNEKNIGEKVLLKGWVQKRRDLGGLIFIDLRDKSGLIQVVFNPDHSKKALE
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHHH
TAENIRSEYVIEINGTVVARDEATINPSMKTGKIEVNASSVQILNKAKTPPFTIQDETDV
HHHHCCCEEEEEECCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCEEECCCCCC
SEDVRLKYRYLDLRRNSLQETFRLRHQTTQSIRNYLNDKDFLEMETPILTKSTPEGARDY
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCCHHC
LVPSRVHPGEFYALPQSPQLFKQLIMMGGFERYYQIARCFRDEDLRADRQPEFTQIDIET
CCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFLTSDEIMDMTEHMMKKVMKEVKDIDISLPLPRMPYQEAMDRYGSDKPDTRFDLELIHV
EECCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCEEEEEEEEH
SDIVASSGFKVFSQAVDNGGKVALLNIKGKADNYSRKDIDKLTEYVKVYDAKGLAWLKAD
HHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
ESELKGPIAKFLSEEEVAGIRDRANVEQGDLLLFVADKTNVVYDSLGALRLYLGKELGLI
HHHHHHHHHHHHCHHHHCCCHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCC
DESKFHFLWVTDWPLLEYDEGLGRYFAAHHPFTSAIEEDLDKLETDPASVRANAYDLVLN
CCCCEEEEEEECCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEEE
GFELGGGSIRIHQKEQQDQMFKVLGFSEEEARSQFGFLLDALEYGAPPHGGIALGLDRII
CEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MLLAGRSNLRDTILFPKTASASDLMTAAPSGVSDDQLQELSIQLQSGEKNQ
HHHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCHHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
SERLMAGRLNEKNIGEKVLLKGWVQKRRDLGGLIFIDLRDKSGLIQVVFNPDHSKKALE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHHH
TAENIRSEYVIEINGTVVARDEATINPSMKTGKIEVNASSVQILNKAKTPPFTIQDETDV
HHHHCCCEEEEEECCEEEECCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCEEECCCCCC
SEDVRLKYRYLDLRRNSLQETFRLRHQTTQSIRNYLNDKDFLEMETPILTKSTPEGARDY
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEECCCCCCCHHC
LVPSRVHPGEFYALPQSPQLFKQLIMMGGFERYYQIARCFRDEDLRADRQPEFTQIDIET
CCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEE
SFLTSDEIMDMTEHMMKKVMKEVKDIDISLPLPRMPYQEAMDRYGSDKPDTRFDLELIHV
EECCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCCCEEEEEEEEH
SDIVASSGFKVFSQAVDNGGKVALLNIKGKADNYSRKDIDKLTEYVKVYDAKGLAWLKAD
HHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECC
ESELKGPIAKFLSEEEVAGIRDRANVEQGDLLLFVADKTNVVYDSLGALRLYLGKELGLI
HHHHHHHHHHHHCHHHHCCCHHHCCCCCCCEEEEEECCCCEEHHHHHHHHHHHCCCCCCC
DESKFHFLWVTDWPLLEYDEGLGRYFAAHHPFTSAIEEDLDKLETDPASVRANAYDLVLN
CCCCEEEEEEECCCCHHHHCCHHHHHHHCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEEE
GFELGGGSIRIHQKEQQDQMFKVLGFSEEEARSQFGFLLDALEYGAPPHGGIALGLDRII
CEEECCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MLLAGRSNLRDTILFPKTASASDLMTAAPSGVSDDQLQELSIQLQSGEKNQ
HHHCCCCCCCCEEEECCCCCCCHHHHCCCCCCCHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376