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The map label for this gene is iscS1 [H]

Identifier: 23099470

GI number: 23099470

Start: 2039802

End: 2040941

Strand: Reverse

Name: iscS1 [H]

Synonym: OB2015

Alternate gene names: 23099470

Gene position: 2040941-2039802 (Counterclockwise)

Preceding gene: 23099471

Following gene: 23099469

Centisome position: 56.22

GC content: 35.79

Gene sequence:

>1140_bases
ATGCAACCTATATATTTAGATCATGCTGCTACTACACCAATTGCCGATGAAGTTATACAGGCTATGATGCCCATTTATCA
GGATGTTGTTGGAAATGCTTCAAGTGTTCATTCATTTGGAAGAAAAGCAAGACAACATTTAGATGGGGCGAGAAGAGTAC
TTGCACAGTCGATTGCTGCTAAAGAAAAGGAAATAATCTTTACAAGTGGTGGAACGGAAGCCGATAATTTAGCATTAATT
GGAACTGCTAAAGCAAATATGCACAAGGGTAGGCATATTATTACCACAGTACAAGAACATCATGCTACACTTCATACGGC
AGAAGAACTTGAGAAACAAGGTTTTACCGTTTCTTATTTACCTGTAGATGAAACTGGACGCGTTAATATAGAAGATTTAG
AACAAGCTTTAACGAATGAGACAATTCTTGTTTCGATAATGTTTGTAAATAATGAAACAGGTGTGATTCAACCAATTGAA
GAAATAGGTAAGCTATTACAGTCACATCAAGCATACTTCCATACGGATGCCGTGCAAGCGTATGGCTTATTAGATATTAA
TGTTGATGAAATGGGTATAGACTTATTAACAACTTCTGCTCATAAAATCAATGGACCAAAAGGAATCGGATTTCTTTATG
CTAGAGAAGGATTAGCTATGAACGCCTTGTCATATGGTGGCGAACAAGAGCGTAAGCGTCGTGCTGGAACAGAAAACATA
CCAGGAGTGGTCGGATTTCAAAAAGCAGTTGAATTATCGATGAATAATAGGGACGAAAGATTGAAAAATTACCAATCCTA
TAAAGAAATTCTCCTACAAACATTAATAGAAAATGAAATTTCATTTTCTATTAATGGAGATAAAGATCATACAATCCCAT
CGATTATTAATATCAGTTTTGATGGGATAAATGTAGAAGCTATGTTAACTAATTTTGATCTTGATGGTGTTGCTGCGTCA
AGTGGAAGTGCATGTACTGCAGGAAGTGTAGAACCTTCTCATGTATTAGCAGCGATGTATGGTGCAGGAAATCCAATTAC
AACTAATTCTATTCGTTTTAGTTTTGGTAGCATGAATACAGTGGAAAACATCCAAGAAGCAAGTATGCGAATCGTTAAGG
TAGTAAATAGATTAAAATAA

Upstream 100 bases:

>100_bases
CAGTTTCATTAAGCTAAGTCTTAGTAAAAAATATTCATACTTTATATCAAGTTTTTAAAGTATGAAATTGATACAGTTAG
ATAGAGAGGGTTTTTTAATC

Downstream 100 bases:

>100_bases
AGGAAGGAACAAACATGAAAAATAACAAAGATACACGTGTAGTTGTTGGAATGAGTGGAGGAGTCGATTCTTCTGTAGCT
GCTTTACTACTTAAACAACA

Product: iron-sulfur cofactor synthesis

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MQPIYLDHAATTPIADEVIQAMMPIYQDVVGNASSVHSFGRKARQHLDGARRVLAQSIAAKEKEIIFTSGGTEADNLALI
GTAKANMHKGRHIITTVQEHHATLHTAEELEKQGFTVSYLPVDETGRVNIEDLEQALTNETILVSIMFVNNETGVIQPIE
EIGKLLQSHQAYFHTDAVQAYGLLDINVDEMGIDLLTTSAHKINGPKGIGFLYAREGLAMNALSYGGEQERKRRAGTENI
PGVVGFQKAVELSMNNRDERLKNYQSYKEILLQTLIENEISFSINGDKDHTIPSIINISFDGINVEAMLTNFDLDGVAAS
SGSACTAGSVEPSHVLAAMYGAGNPITTNSIRFSFGSMNTVENIQEASMRIVKVVNRLK

Sequences:

>Translated_379_residues
MQPIYLDHAATTPIADEVIQAMMPIYQDVVGNASSVHSFGRKARQHLDGARRVLAQSIAAKEKEIIFTSGGTEADNLALI
GTAKANMHKGRHIITTVQEHHATLHTAEELEKQGFTVSYLPVDETGRVNIEDLEQALTNETILVSIMFVNNETGVIQPIE
EIGKLLQSHQAYFHTDAVQAYGLLDINVDEMGIDLLTTSAHKINGPKGIGFLYAREGLAMNALSYGGEQERKRRAGTENI
PGVVGFQKAVELSMNNRDERLKNYQSYKEILLQTLIENEISFSINGDKDHTIPSIINISFDGINVEAMLTNFDLDGVAAS
SGSACTAGSVEPSHVLAAMYGAGNPITTNSIRFSFGSMNTVENIQEASMRIVKVVNRLK
>Mature_379_residues
MQPIYLDHAATTPIADEVIQAMMPIYQDVVGNASSVHSFGRKARQHLDGARRVLAQSIAAKEKEIIFTSGGTEADNLALI
GTAKANMHKGRHIITTVQEHHATLHTAEELEKQGFTVSYLPVDETGRVNIEDLEQALTNETILVSIMFVNNETGVIQPIE
EIGKLLQSHQAYFHTDAVQAYGLLDINVDEMGIDLLTTSAHKINGPKGIGFLYAREGLAMNALSYGGEQERKRRAGTENI
PGVVGFQKAVELSMNNRDERLKNYQSYKEILLQTLIENEISFSINGDKDHTIPSIINISFDGINVEAMLTNFDLDGVAAS
SGSACTAGSVEPSHVLAAMYGAGNPITTNSIRFSFGSMNTVENIQEASMRIVKVVNRLK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=382, Percent_Identity=40.8376963350785, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI156713448, Length=394, Percent_Identity=34.010152284264, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=40.5194805194805, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789175, Length=393, Percent_Identity=25.1908396946565, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787970, Length=348, Percent_Identity=26.7241379310345, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=44.4444444444444, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=29.483282674772, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=42.4083769633508, Blast_Score=285, Evalue=7e-78,
Organism=Drosophila melanogaster, GI20129463, Length=386, Percent_Identity=42.2279792746114, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41370; Mature: 41370

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPIYLDHAATTPIADEVIQAMMPIYQDVVGNASSVHSFGRKARQHLDGARRVLAQSIAA
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KEKEIIFTSGGTEADNLALIGTAKANMHKGRHIITTVQEHHATLHTAEELEKQGFTVSYL
CCCEEEEECCCCCCCCEEEEEECCHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEE
PVDETGRVNIEDLEQALTNETILVSIMFVNNETGVIQPIEEIGKLLQSHQAYFHTDAVQA
ECCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YGLLDINVDEMGIDLLTTSAHKINGPKGIGFLYAREGLAMNALSYGGEQERKRRAGTENI
EEEEEECHHHHCCEEEECCCHHCCCCCCCEEEEECCCCEEHHHHCCCHHHHHHHCCCCCC
PGVVGFQKAVELSMNNRDERLKNYQSYKEILLQTLIENEISFSINGDKDHTIPSIINISF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEE
DGINVEAMLTNFDLDGVAASSGSACTAGSVEPSHVLAAMYGAGNPITTNSIRFSFGSMNT
CCEEEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCH
VENIQEASMRIVKVVNRLK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPIYLDHAATTPIADEVIQAMMPIYQDVVGNASSVHSFGRKARQHLDGARRVLAQSIAA
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KEKEIIFTSGGTEADNLALIGTAKANMHKGRHIITTVQEHHATLHTAEELEKQGFTVSYL
CCCEEEEECCCCCCCCEEEEEECCHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEE
PVDETGRVNIEDLEQALTNETILVSIMFVNNETGVIQPIEEIGKLLQSHQAYFHTDAVQA
ECCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YGLLDINVDEMGIDLLTTSAHKINGPKGIGFLYAREGLAMNALSYGGEQERKRRAGTENI
EEEEEECHHHHCCEEEECCCHHCCCCCCCEEEEECCCCEEHHHHCCCHHHHHHHCCCCCC
PGVVGFQKAVELSMNNRDERLKNYQSYKEILLQTLIENEISFSINGDKDHTIPSIINISF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEEE
DGINVEAMLTNFDLDGVAASSGSACTAGSVEPSHVLAAMYGAGNPITTNSIRFSFGSMNT
CCEEEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCH
VENIQEASMRIVKVVNRLK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]