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The map label for this gene is cobA [H]

Identifier: 23099110

GI number: 23099110

Start: 1699043

End: 1699816

Strand: Direct

Name: cobA [H]

Synonym: OB1655

Alternate gene names: 23099110

Gene position: 1699043-1699816 (Clockwise)

Preceding gene: 23099109

Following gene: 23099111

Centisome position: 46.8

GC content: 40.83

Gene sequence:

>774_bases
ATGGGGAAGGTTTATTTAGTTGGAGCCGGTCCTGGTGACCCGGAACTGATTACGCTCAAAGGCTTAAAAGCAATTCAGCA
ATCTGACATTATCTTATATGACAGACTAGTGAATGAGGAGTTATTAGGCTACGCTTCTAAGAATGCCGAGTTGATATATT
GTGGTAAGAGTCCAGAACACCATTCACTTACGCAGGACAATATAAACAAATTACTCTGCGCATATGCGAAGGAGGATAAA
ATTGTCACGAGACTAAAAGGCGGTGATCCGTTTATCTTTGGGAGAGGTGGGGAAGAAGCGATGGTTCTAAGAGAAAATGG
ATTGATTTTTGAAATCATTCCTGGAATTACTTCTGGTTCCGCTGCTCCTACCTATGCTGGTATACCTTTAACCCATCGGG
ACTACAGTTCTTCCGTATCTTTTGTATCTGGGGTTAGTAAAACTGGTGAGGAGCATGAAAGATATTGGGAACATCTTGCG
AAAAGTTCAGATACTCTTTGCATTTATATGGGAGTGAAAAGCTTACCAGAGATTTGTGAACGATTAATCCGCTATGGCCG
TCATTCCACAACACCAATTGCAGTTATTCATTGGGGAACGACTGAAATGCAACAAACCGTCACAGGGACATTGGAAGATA
TCACCCATGTTACAGAAAAGTTAAAAAATCCTTCCATGATTATTATCGGTGAAGTAGTGCGACTTAGGGATGAGATTCAA
TGGTTTAATGAGGAAACGATAGAACACTTTAATCCAGAAGTGGTTATTGGATAG

Upstream 100 bases:

>100_bases
TCGTGCTGGATACGTGGAAGAAGTTAAATCTGGTTTGGATTTTCATTCTAGCAAGGAAGACGTACGTAGTTGATAACGAC
TCTGGAAGGGAAGTGAAAGA

Downstream 100 bases:

>100_bases
TTGGGAGGTGCAGTTGTGCAAGGGGTGCTTTATGTCAGTCACGGTAGTCGTATACCTGAAGCAACAGCAGATGCAATTGC
TTTTATTAATCAAGTGAAAA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MGKVYLVGAGPGDPELITLKGLKAIQQSDIILYDRLVNEELLGYASKNAELIYCGKSPEHHSLTQDNINKLLCAYAKEDK
IVTRLKGGDPFIFGRGGEEAMVLRENGLIFEIIPGITSGSAAPTYAGIPLTHRDYSSSVSFVSGVSKTGEEHERYWEHLA
KSSDTLCIYMGVKSLPEICERLIRYGRHSTTPIAVIHWGTTEMQQTVTGTLEDITHVTEKLKNPSMIIIGEVVRLRDEIQ
WFNEETIEHFNPEVVIG

Sequences:

>Translated_257_residues
MGKVYLVGAGPGDPELITLKGLKAIQQSDIILYDRLVNEELLGYASKNAELIYCGKSPEHHSLTQDNINKLLCAYAKEDK
IVTRLKGGDPFIFGRGGEEAMVLRENGLIFEIIPGITSGSAAPTYAGIPLTHRDYSSSVSFVSGVSKTGEEHERYWEHLA
KSSDTLCIYMGVKSLPEICERLIRYGRHSTTPIAVIHWGTTEMQQTVTGTLEDITHVTEKLKNPSMIIIGEVVRLRDEIQ
WFNEETIEHFNPEVVIG
>Mature_256_residues
GKVYLVGAGPGDPELITLKGLKAIQQSDIILYDRLVNEELLGYASKNAELIYCGKSPEHHSLTQDNINKLLCAYAKEDKI
VTRLKGGDPFIFGRGGEEAMVLRENGLIFEIIPGITSGSAAPTYAGIPLTHRDYSSSVSFVSGVSKTGEEHERYWEHLAK
SSDTLCIYMGVKSLPEICERLIRYGRHSTTPIAVIHWGTTEMQQTVTGTLEDITHVTEKLKNPSMIIIGEVVRLRDEIQW
FNEETIEHFNPEVVIG

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=45.8677685950413, Blast_Score=238, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6322922, Length=254, Percent_Identity=28.740157480315, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 28576; Mature: 28445

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVYLVGAGPGDPELITLKGLKAIQQSDIILYDRLVNEELLGYASKNAELIYCGKSPEH
CCEEEEEECCCCCCCEEEECCHHHHHHCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCC
HSLTQDNINKLLCAYAKEDKIVTRLKGGDPFIFGRGGEEAMVLRENGLIFEIIPGITSGS
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCEEEEECCCEEEEEECCCCCCC
AAPTYAGIPLTHRDYSSSVSFVSGVSKTGEEHERYWEHLAKSSDTLCIYMGVKSLPEICE
CCCCCCCCCEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
RLIRYGRHSTTPIAVIHWGTTEMQQTVTGTLEDITHVTEKLKNPSMIIIGEVVRLRDEIQ
HHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
WFNEETIEHFNPEVVIG
HHCHHHHHHCCCCEEEC
>Mature Secondary Structure 
GKVYLVGAGPGDPELITLKGLKAIQQSDIILYDRLVNEELLGYASKNAELIYCGKSPEH
CEEEEEECCCCCCCEEEECCHHHHHHCCEEEEHHHHHHHHHHHCCCCCEEEEECCCCCC
HSLTQDNINKLLCAYAKEDKIVTRLKGGDPFIFGRGGEEAMVLRENGLIFEIIPGITSGS
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCEEEEECCCEEEEEECCCCCCC
AAPTYAGIPLTHRDYSSSVSFVSGVSKTGEEHERYWEHLAKSSDTLCIYMGVKSLPEICE
CCCCCCCCCEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHH
RLIRYGRHSTTPIAVIHWGTTEMQQTVTGTLEDITHVTEKLKNPSMIIIGEVVRLRDEIQ
HHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
WFNEETIEHFNPEVVIG
HHCHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1906874 [H]