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The map label for this gene is cysI

Identifier: 23099109

GI number: 23099109

Start: 1697285

End: 1699015

Strand: Direct

Name: cysI

Synonym: OB1654

Alternate gene names: 23099109

Gene position: 1697285-1699015 (Clockwise)

Preceding gene: 23099108

Following gene: 23099110

Centisome position: 46.75

GC content: 40.73

Gene sequence:

>1731_bases
GTGACGAAATCTAATATTTCTAAAGATAACGGTCCATTGGATGAAGTGGAGGGTATTAAGGAGAGAAGTAACTTTTTGCG
GGGCACCATTGCAGAGGGATTGGAAGACCGAATTACCGGAGCGATTTCAGATGAAGATAATAAACTTTTAAAGTTTCACG
GGAGCTATCAACAGGATGATAGGGATTTACGTGATGAAAGACGTCGTAAAAAACTAGAACCGGCATACCAGTTTATGATT
CGTGTGCGTACTCCAGGAGGTGTCACGACGCCTGAACAGTGGCTGGTGATGGATGACTTAGCTCATAAATATGCAAATGG
ATCAATGCGGTTAACCACCAGACAAACATTTCAACTACATGGCATTGTGAAATGGGATTTGAAGAAGACCATGCAGGGTA
TGAATCAGGCATTGATGACCACAATTGCAGCATGTGGGGATATAAATAGGAATGTCATGTGTAATGTTAATCCAGAACAG
TCAGATGTACATGCAGAGGTATATGAGTGGTCGAAGAAAGTTAGTGATCACTTGTTACCGGAGACCAATGCTTATCATGA
AATTTGGTTGGAAGACAAGAAGATTGTAGATAGCCGTGAAGAAAGTGAACCAATCTATGGATCGACCTACCTTCCTAGGA
AATTCAAGATTGGTATTGCGGTTCCTCCATCGAATGATATCGACATTTATTCTCAAGATTTGGGCTTTATAGCAATCGTG
GAGGAAGGGAAATTGTTGGGCTTTAATGTTGCTGTTGGCGGGGGTATGGGAATGACCCATGGTGATACAAATACCTATCC
ACAGATATCTCGGGTAATCGGGTTTTGTGTGCCTGAGAAGGTCACCGAAGTTGCTGAAAAAGTAGTAACGATTCAAAGGG
ATTTTGGAAACCGTTCGAACAGAAAAAATGCTCGTTTTAAATATACGATTGATAGCAGAGGAATAGATTGGTTTATAGAT
GAATTGAACGAAAGGTTAGGTTGGGAGCTAGAAGGCAAACGACACTATGAATTTGATTCTAATGGCGACAGTTATGGATG
GATAGAAGGAAAAGATTATTGGCATCTAACCTTATTTATTCAGAATGGCCGAATAAAAGATGTTGAGGATTATCCGTTAA
TGACGGGGTTACGAGAAATTGCAAAGGTGCATACAGGCGACTTCCGTCTGACTCCGAATCAAAATCTTATAATCGCAAAT
GTATCCAGTGATAACAAGAAACAAATCAATGATCTGGTAGAAAAATTTAATCTTACTGAAGGAAAGACATATTCCGGGCT
TAGGAGAAATTCAATGGCATGTGTTGCATTCCCAACATGTGGGCTTGCCATGGCGGAATCAGAAAGGTACTTGCCGGAGC
TTTTGGATAAAATCGAGGTCATACTTGACGAATCAGGTCTTCGTGAGGAAGAAATAGTCATCCGTATGTCAGGTTGTCCA
AATGGTTGTTCACGTCCTGCATTGGCCGAAATCGCCTTTATTGGAAAAGCTCCTGGAAAATACAATATGTATTTGGGCGG
GAGCTTTAATGGAAGTCGGCTTAACAAACTTTACAAGGAAAATATAGGCGAAGAGGAGATTCTGAACACATTGCGCCCTA
TATTTATAGACTTTGCTAAGGATAAACAGAAAGATGAACACTTTGGTGATTTTGTGATTCGTGCTGGATACGTGGAAGAA
GTTAAATCTGGTTTGGATTTTCATTCTAGCAAGGAAGACGTACGTAGTTGA

Upstream 100 bases:

>100_bases
GTTTTGAGGAAGCAGAAGCATATTTGACTGATATGCGAAAACAGAAACGATACCAACGAGACGTGTATTGAAATAAGTAT
ACGAAAAAGGAGTTGAAAGA

Downstream 100 bases:

>100_bases
TAACGACTCTGGAAGGGAAGTGAAAGAATGGGGAAGGTTTATTTAGTTGGAGCCGGTCCTGGTGACCCGGAACTGATTAC
GCTCAAAGGCTTAAAAGCAA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MTKSNISKDNGPLDEVEGIKERSNFLRGTIAEGLEDRITGAISDEDNKLLKFHGSYQQDDRDLRDERRRKKLEPAYQFMI
RVRTPGGVTTPEQWLVMDDLAHKYANGSMRLTTRQTFQLHGIVKWDLKKTMQGMNQALMTTIAACGDINRNVMCNVNPEQ
SDVHAEVYEWSKKVSDHLLPETNAYHEIWLEDKKIVDSREESEPIYGSTYLPRKFKIGIAVPPSNDIDIYSQDLGFIAIV
EEGKLLGFNVAVGGGMGMTHGDTNTYPQISRVIGFCVPEKVTEVAEKVVTIQRDFGNRSNRKNARFKYTIDSRGIDWFID
ELNERLGWELEGKRHYEFDSNGDSYGWIEGKDYWHLTLFIQNGRIKDVEDYPLMTGLREIAKVHTGDFRLTPNQNLIIAN
VSSDNKKQINDLVEKFNLTEGKTYSGLRRNSMACVAFPTCGLAMAESERYLPELLDKIEVILDESGLREEEIVIRMSGCP
NGCSRPALAEIAFIGKAPGKYNMYLGGSFNGSRLNKLYKENIGEEEILNTLRPIFIDFAKDKQKDEHFGDFVIRAGYVEE
VKSGLDFHSSKEDVRS

Sequences:

>Translated_576_residues
MTKSNISKDNGPLDEVEGIKERSNFLRGTIAEGLEDRITGAISDEDNKLLKFHGSYQQDDRDLRDERRRKKLEPAYQFMI
RVRTPGGVTTPEQWLVMDDLAHKYANGSMRLTTRQTFQLHGIVKWDLKKTMQGMNQALMTTIAACGDINRNVMCNVNPEQ
SDVHAEVYEWSKKVSDHLLPETNAYHEIWLEDKKIVDSREESEPIYGSTYLPRKFKIGIAVPPSNDIDIYSQDLGFIAIV
EEGKLLGFNVAVGGGMGMTHGDTNTYPQISRVIGFCVPEKVTEVAEKVVTIQRDFGNRSNRKNARFKYTIDSRGIDWFID
ELNERLGWELEGKRHYEFDSNGDSYGWIEGKDYWHLTLFIQNGRIKDVEDYPLMTGLREIAKVHTGDFRLTPNQNLIIAN
VSSDNKKQINDLVEKFNLTEGKTYSGLRRNSMACVAFPTCGLAMAESERYLPELLDKIEVILDESGLREEEIVIRMSGCP
NGCSRPALAEIAFIGKAPGKYNMYLGGSFNGSRLNKLYKENIGEEEILNTLRPIFIDFAKDKQKDEHFGDFVIRAGYVEE
VKSGLDFHSSKEDVRS
>Mature_575_residues
TKSNISKDNGPLDEVEGIKERSNFLRGTIAEGLEDRITGAISDEDNKLLKFHGSYQQDDRDLRDERRRKKLEPAYQFMIR
VRTPGGVTTPEQWLVMDDLAHKYANGSMRLTTRQTFQLHGIVKWDLKKTMQGMNQALMTTIAACGDINRNVMCNVNPEQS
DVHAEVYEWSKKVSDHLLPETNAYHEIWLEDKKIVDSREESEPIYGSTYLPRKFKIGIAVPPSNDIDIYSQDLGFIAIVE
EGKLLGFNVAVGGGMGMTHGDTNTYPQISRVIGFCVPEKVTEVAEKVVTIQRDFGNRSNRKNARFKYTIDSRGIDWFIDE
LNERLGWELEGKRHYEFDSNGDSYGWIEGKDYWHLTLFIQNGRIKDVEDYPLMTGLREIAKVHTGDFRLTPNQNLIIANV
SSDNKKQINDLVEKFNLTEGKTYSGLRRNSMACVAFPTCGLAMAESERYLPELLDKIEVILDESGLREEEIVIRMSGCPN
GCSRPALAEIAFIGKAPGKYNMYLGGSFNGSRLNKLYKENIGEEEILNTLRPIFIDFAKDKQKDEHFGDFVIRAGYVEEV
KSGLDFHSSKEDVRS

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=553, Percent_Identity=50.0904159132007, Blast_Score=576, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6322597, Length=591, Percent_Identity=46.8697123519459, Blast_Score=575, Evalue=1e-164,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_OCEIH (Q8EQP0)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692575.1
- HSSP:   P17846
- ProteinModelPortal:   Q8EQP0
- SMR:   Q8EQP0
- GeneID:   1018150
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1654
- NMPDR:   fig|221109.1.peg.1657
- HOGENOM:   HBG626671
- OMA:   ESERVFP
- ProtClustDB:   PRK13504
- BioCyc:   OIHE221109:OB1654-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 65554; Mature: 65423

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSNISKDNGPLDEVEGIKERSNFLRGTIAEGLEDRITGAISDEDNKLLKFHGSYQQDD
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCH
RDLRDERRRKKLEPAYQFMIRVRTPGGVTTPEQWLVMDDLAHKYANGSMRLTTRQTFQLH
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
GIVKWDLKKTMQGMNQALMTTIAACGDINRNVMCNVNPEQSDVHAEVYEWSKKVSDHLLP
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
ETNAYHEIWLEDKKIVDSREESEPIYGSTYLPRKFKIGIAVPPSNDIDIYSQDLGFIAIV
CCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEE
EEGKLLGFNVAVGGGMGMTHGDTNTYPQISRVIGFCVPEKVTEVAEKVVTIQRDFGNRSN
ECCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
RKNARFKYTIDSRGIDWFIDELNERLGWELEGKRHYEFDSNGDSYGWIEGKDYWHLTLFI
CCCCEEEEEECCCCCHHHHHHHHHHHCCEECCCEEECCCCCCCCEEEECCCCEEEEEEEE
QNGRIKDVEDYPLMTGLREIAKVHTGDFRLTPNQNLIIANVSSDNKKQINDLVEKFNLTE
ECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCC
GKTYSGLRRNSMACVAFPTCGLAMAESERYLPELLDKIEVILDESGLREEEIVIRMSGCP
CCCCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
NGCSRPALAEIAFIGKAPGKYNMYLGGSFNGSRLNKLYKENIGEEEILNTLRPIFIDFAK
CCCCCCHHHHEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DKQKDEHFGDFVIRAGYVEEVKSGLDFHSSKEDVRS
CCCCHHHHCCCEEECCCHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure 
TKSNISKDNGPLDEVEGIKERSNFLRGTIAEGLEDRITGAISDEDNKLLKFHGSYQQDD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCH
RDLRDERRRKKLEPAYQFMIRVRTPGGVTTPEQWLVMDDLAHKYANGSMRLTTRQTFQLH
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEE
GIVKWDLKKTMQGMNQALMTTIAACGDINRNVMCNVNPEQSDVHAEVYEWSKKVSDHLLP
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCC
ETNAYHEIWLEDKKIVDSREESEPIYGSTYLPRKFKIGIAVPPSNDIDIYSQDLGFIAIV
CCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEE
EEGKLLGFNVAVGGGMGMTHGDTNTYPQISRVIGFCVPEKVTEVAEKVVTIQRDFGNRSN
ECCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
RKNARFKYTIDSRGIDWFIDELNERLGWELEGKRHYEFDSNGDSYGWIEGKDYWHLTLFI
CCCCEEEEEECCCCCHHHHHHHHHHHCCEECCCEEECCCCCCCCEEEECCCCEEEEEEEE
QNGRIKDVEDYPLMTGLREIAKVHTGDFRLTPNQNLIIANVSSDNKKQINDLVEKFNLTE
ECCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHCCCCC
GKTYSGLRRNSMACVAFPTCGLAMAESERYLPELLDKIEVILDESGLREEEIVIRMSGCP
CCCCCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
NGCSRPALAEIAFIGKAPGKYNMYLGGSFNGSRLNKLYKENIGEEEILNTLRPIFIDFAK
CCCCCCHHHHEEEEECCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
DKQKDEHFGDFVIRAGYVEEVKSGLDFHSSKEDVRS
CCCCHHHHCCCEEECCCHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376