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The map label for this gene is murE

Identifier: 23098921

GI number: 23098921

Start: 1504802

End: 1506283

Strand: Direct

Name: murE

Synonym: OB1466

Alternate gene names: 23098921

Gene position: 1504802-1506283 (Clockwise)

Preceding gene: 23098920

Following gene: 23098922

Centisome position: 41.45

GC content: 36.3

Gene sequence:

>1482_bases
ATGGAATTAAAAAAATTACTAGAATGTCTTACATTTTATAATGTGAATGGTGAAGTGGAAAATTGTGAAATTACTTCACT
AGAAATGGATTCGAGAAAAATAACTAGCGGAAGTGCATTTGTATGTATCACGGGATTTACTGTGGATGGACATGATTATG
TAGATCAAGCGGTGAAAAATGGAGCATCTGCAATATTTACTAGTAAACCTTTAATGAAGGAATATGGGGTTCCAATCATT
CAGGTGGAGGATACGAATCGCGCACTTGCGATGCTTGCAGTGAAGTATTATGATTACCCAACCAAACATTTTCCGCTTAT
CGGTGTTACTGGAACAAACGGAAAAACAACTGTTACTTATTTGTTAGATAAAATTTTTGAATATCATCAGAAAAAAGCTG
GAGTAATTGGAACAATTCAAGTGAAAATAGGGGAAGAGACCTTCCCTATTGTAAATACAACTCCTAACGCACTTGAGCTA
CAAAAAACATTTCATGTAATGCGTGAAAAAGATGTGAAACAGGGTATCATGGAAGTTTCCTCTCATGCGTTAGATATGGG
AAGGGTATATGGATGTGATTATGATATTGCGGTATTTACCAATCTTTCCCAAGATCATTTAGATTATCACCAAGATATTC
AAGATTATTTACGTGCGAAAAGCTTGTTGTTTGCTCAACTAGGTAATGGCTACGACAGTGAAAAAGAAAAATATGCAATC
ATTAATGATGATGATTCATCAAGTCATTTATTAAAAAGAAGTACTGCACAACATGTCATAACGTATAGCTGTAAAAAAGA
AGCGACTATTATGGCAAAAGATATTGAACTTACAGCATCTGGTATTCGTTTTAAATTACATTCCCCTCTAGGTAACATCA
CAATTCAAAGTAGATTAATGGGAATGTTTAATGTTTATAATATGTTAGCTGCAAGTGCGGCAGCTATCGCATCGAAGGTT
CCATTGAACGTCATCCAACAAGCATTAGAATCAATAGAGGGAGTAAATGGCAGGTTCGAACCAATTGTCGAAGGACAAAA
TTATTCTGTAATTGTGGATTTTGCACATACTCCAGATTCATTAGAAAACGTCCTACAGACAATAAAGGACTTTGCAAAAC
GAAATGTATATGTGGTCGTAGGATGTGGTGGAGATCGAGATCGTAAGAAAAGACCTCTGATGGCTGAAGTGGCGTTGAAT
TATGCGGATCATGCGGTGTTCACTTCTGACAATCCGCGTACGGAAGACCCTCAAGCAATATTAGATGATATGACTGCTGA
ACTAGATGCAAATAGTGGTAGTTATGAGGTTGTGGTAGATCGTAAAGAAGGAATTGCTAAAGCAATACAATCTGCTCAAA
AAGATGATATTGTTTTAATCGCTGGAAAAGGTCATGAGACATATCAAATTATTGGACATACGAAATACGATTTCGACGAT
AGAGATGTGGCTAGAAATGCAATTAAGCAGAAAGGGGAGTGA

Upstream 100 bases:

>100_bases
AGGAAGGTCAAATGGAATGAAAAAGAAGAGTACTTACATCTTCTTCCTACTAAAAAAAGAAAGTACCTAAATAGTATTAA
TTAGAAAAAAGGTGTATATG

Downstream 100 bases:

>100_bases
ACCTATTGTTATTTACAGTACAATGGCTGGCGGAATTATTTCCTAAACATCAGGGAAAAATAAGTACAGACATTACTATA
CAGGAAGTAACTCGTGATAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase 1; UDP-N-acetylmuramyl-tripeptide synthetase 1

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MELKKLLECLTFYNVNGEVENCEITSLEMDSRKITSGSAFVCITGFTVDGHDYVDQAVKNGASAIFTSKPLMKEYGVPII
QVEDTNRALAMLAVKYYDYPTKHFPLIGVTGTNGKTTVTYLLDKIFEYHQKKAGVIGTIQVKIGEETFPIVNTTPNALEL
QKTFHVMREKDVKQGIMEVSSHALDMGRVYGCDYDIAVFTNLSQDHLDYHQDIQDYLRAKSLLFAQLGNGYDSEKEKYAI
INDDDSSSHLLKRSTAQHVITYSCKKEATIMAKDIELTASGIRFKLHSPLGNITIQSRLMGMFNVYNMLAASAAAIASKV
PLNVIQQALESIEGVNGRFEPIVEGQNYSVIVDFAHTPDSLENVLQTIKDFAKRNVYVVVGCGGDRDRKKRPLMAEVALN
YADHAVFTSDNPRTEDPQAILDDMTAELDANSGSYEVVVDRKEGIAKAIQSAQKDDIVLIAGKGHETYQIIGHTKYDFDD
RDVARNAIKQKGE

Sequences:

>Translated_493_residues
MELKKLLECLTFYNVNGEVENCEITSLEMDSRKITSGSAFVCITGFTVDGHDYVDQAVKNGASAIFTSKPLMKEYGVPII
QVEDTNRALAMLAVKYYDYPTKHFPLIGVTGTNGKTTVTYLLDKIFEYHQKKAGVIGTIQVKIGEETFPIVNTTPNALEL
QKTFHVMREKDVKQGIMEVSSHALDMGRVYGCDYDIAVFTNLSQDHLDYHQDIQDYLRAKSLLFAQLGNGYDSEKEKYAI
INDDDSSSHLLKRSTAQHVITYSCKKEATIMAKDIELTASGIRFKLHSPLGNITIQSRLMGMFNVYNMLAASAAAIASKV
PLNVIQQALESIEGVNGRFEPIVEGQNYSVIVDFAHTPDSLENVLQTIKDFAKRNVYVVVGCGGDRDRKKRPLMAEVALN
YADHAVFTSDNPRTEDPQAILDDMTAELDANSGSYEVVVDRKEGIAKAIQSAQKDDIVLIAGKGHETYQIIGHTKYDFDD
RDVARNAIKQKGE
>Mature_493_residues
MELKKLLECLTFYNVNGEVENCEITSLEMDSRKITSGSAFVCITGFTVDGHDYVDQAVKNGASAIFTSKPLMKEYGVPII
QVEDTNRALAMLAVKYYDYPTKHFPLIGVTGTNGKTTVTYLLDKIFEYHQKKAGVIGTIQVKIGEETFPIVNTTPNALEL
QKTFHVMREKDVKQGIMEVSSHALDMGRVYGCDYDIAVFTNLSQDHLDYHQDIQDYLRAKSLLFAQLGNGYDSEKEKYAI
INDDDSSSHLLKRSTAQHVITYSCKKEATIMAKDIELTASGIRFKLHSPLGNITIQSRLMGMFNVYNMLAASAAAIASKV
PLNVIQQALESIEGVNGRFEPIVEGQNYSVIVDFAHTPDSLENVLQTIKDFAKRNVYVVVGCGGDRDRKKRPLMAEVALN
YADHAVFTSDNPRTEDPQAILDDMTAELDANSGSYEVVVDRKEGIAKAIQSAQKDDIVLIAGKGHETYQIIGHTKYDFDD
RDVARNAIKQKGE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=477, Percent_Identity=37.7358490566038, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1786274, Length=372, Percent_Identity=25.2688172043011, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE1_OCEIH (Q8CZE6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692387.1
- ProteinModelPortal:   Q8CZE6
- SMR:   Q8CZE6
- GeneID:   1017713
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1466
- NMPDR:   fig|221109.1.peg.1468
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- BioCyc:   OIHE221109:OB1466-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54817; Mature: 54817

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: BINDING 31-31 BINDING 152-152 BINDING 180-180 BINDING 188-188 BINDING 386-386 BINDING 462-462 BINDING 466-466

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKKLLECLTFYNVNGEVENCEITSLEMDSRKITSGSAFVCITGFTVDGHDYVDQAVKN
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEECCCCEEEEEEEEEECCHHHHHHHHHC
GASAIFTSKPLMKEYGVPIIQVEDTNRALAMLAVKYYDYPTKHFPLIGVTGTNGKTTVTY
CCCEEEECCCHHHHHCCCEEEEECCCCEEEHEEHHHCCCCCCCCCEEEEECCCCCCHHHH
LLDKIFEYHQKKAGVIGTIQVKIGEETFPIVNTTPNALELQKTFHVMREKDVKQGIMEVS
HHHHHHHHHHHCCCEEEEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SHALDMGRVYGCDYDIAVFTNLSQDHLDYHQDIQDYLRAKSLLFAQLGNGYDSEKEKYAI
HHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
INDDDSSSHLLKRSTAQHVITYSCKKEATIMAKDIELTASGIRFKLHSPLGNITIQSRLM
ECCCCCCHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEECCEEEEEECCCCCEEHHHHHH
GMFNVYNMLAASAAAIASKVPLNVIQQALESIEGVNGRFEPIVEGQNYSVIVDFAHTPDS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCHH
LENVLQTIKDFAKRNVYVVVGCGGDRDRKKRPLMAEVALNYADHAVFTSDNPRTEDPQAI
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHH
LDDMTAELDANSGSYEVVVDRKEGIAKAIQSAQKDDIVLIAGKGHETYQIIGHTKYDFDD
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCH
RDVARNAIKQKGE
HHHHHHHHHHCCC
>Mature Secondary Structure
MELKKLLECLTFYNVNGEVENCEITSLEMDSRKITSGSAFVCITGFTVDGHDYVDQAVKN
CCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEECCCCEEEEEEEEEECCHHHHHHHHHC
GASAIFTSKPLMKEYGVPIIQVEDTNRALAMLAVKYYDYPTKHFPLIGVTGTNGKTTVTY
CCCEEEECCCHHHHHCCCEEEEECCCCEEEHEEHHHCCCCCCCCCEEEEECCCCCCHHHH
LLDKIFEYHQKKAGVIGTIQVKIGEETFPIVNTTPNALELQKTFHVMREKDVKQGIMEVS
HHHHHHHHHHHCCCEEEEEEEEECCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SHALDMGRVYGCDYDIAVFTNLSQDHLDYHQDIQDYLRAKSLLFAQLGNGYDSEKEKYAI
HHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
INDDDSSSHLLKRSTAQHVITYSCKKEATIMAKDIELTASGIRFKLHSPLGNITIQSRLM
ECCCCCCHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEECCEEEEEECCCCCEEHHHHHH
GMFNVYNMLAASAAAIASKVPLNVIQQALESIEGVNGRFEPIVEGQNYSVIVDFAHTPDS
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCCHH
LENVLQTIKDFAKRNVYVVVGCGGDRDRKKRPLMAEVALNYADHAVFTSDNPRTEDPQAI
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCHHHH
LDDMTAELDANSGSYEVVVDRKEGIAKAIQSAQKDDIVLIAGKGHETYQIIGHTKYDFDD
HHHHHHHCCCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCCH
RDVARNAIKQKGE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376