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The map label for this gene is murF [H]

Identifier: 23098922

GI number: 23098922

Start: 1506289

End: 1507656

Strand: Direct

Name: murF [H]

Synonym: OB1467

Alternate gene names: 23098922

Gene position: 1506289-1507656 (Clockwise)

Preceding gene: 23098921

Following gene: 23098923

Centisome position: 41.49

GC content: 33.77

Gene sequence:

>1368_bases
TTGTTATTTACAGTACAATGGCTGGCGGAATTATTTCCTAAACATCAGGGAAAAATAAGTACAGACATTACTATACAGGA
AGTAACTCGTGATACAAGAGAACCAAGTTCCAACTCCCTATATATTCCGATTGTTGGAGAAAGATTTGATGGACATAATT
TCATTTTTGATGCAATTAAAAATGGTGCGATCGCAACATTATGGGATAAGTCCATTCCTTTGCCCAGTGAATGGGACAAC
TCCTTTCCTGTATTTTTCGTAGAGGATACGATTTTAGCTATGCAACAGTTAGCTAATTCCTATCTTAGATCTGTTAATCC
CACTGTAGTTGGTGTTACGGGGTCTAATGGTAAGACCACAACGAAAGATTTGATTACGTCGGTGGTAAAGACAAGCTATA
AAACAACAGCTACACAGGGTAATTTAAATAACCATATTGGATTGCCACTTACTATTTTATCGATGCAACCTGATACTGAA
GTACTCGTACTTGAGATGGGTATGAGTCAGTTTGGAGAAATTGAGTTATTAAGTAAAATAGCGGAACCAGACTACACAGT
AATTACGAATATTGGTGAATCTCATATTGAAAATTTAGGTTCCCGAAAAGGTATTGCAAAAGCAAAATTGGAAATAATTG
AAGGAATAAAGTCAAACGGTAATTTATTTATTGATGATGACGAGCCTTTATTAGCGAAAGAAAATATAGATATGGATGAT
TCGCAAATAATTGGAATTGGCTTTACGAAAGAAAGTGACATTAACATTTCGAATGTAAAAGTGCATCCTGATCGTACTAC
ATTTGAAGTAACGGATAAACCATATCATGTTAATCTGCTCGGTGAACACCATGCAAAGAATGCAGCATTCGCCATTTCTA
TTGGGAAAGCTCTAAATATTTCACATGAGAATATACAATTAGCTTTTAATAAGCTAGAAGTTACTGGAATGCGTTTTGAA
CTTGAAACAGGGATTAATGGTGTCCATATCATAAATGATGCATATAATGCCTCTGCCACTTCCATGAAAGCGTCTATTCA
AGTAGTTAAAGAAATGGAAGGTTTTCAAAATAAGGTATTAGTGTTGGGGGATATTTTAGAGTTAGGTACATATTCAGAAC
ACTATCATCGTTCGATTGCCGAAGTTATTGATTCCCGAATTTCAGAAATTTATACTTATGGAGAGCAAGCTTTTTATATT
TATGATGAAATTACAAAGCAACATCCTAAAGTAAATGTACATTATATAAAACAACGAGAAGATGTTGTTCCGTCATTAAA
GGAGCATTTAAATAATCAAACATTAATATTATTTAAAGCTTCTAGAGGAATGAAGTTTGAAATGTTTATTAAAGAATTAC
AGCAGTAA

Upstream 100 bases:

>100_bases
AAGGTCATGAGACATATCAAATTATTGGACATACGAAATACGATTTCGACGATAGAGATGTGGCTAGAAATGCAATTAAG
CAGAAAGGGGAGTGAACCTA

Downstream 100 bases:

>100_bases
TCGAACATCTAGTTACTTAGTTGGACGGTTTAATAAGTGAGGAGGAAATGAAGTGGACTTAACTGGATTATTAATAACAA
TAGTTGTAGCATTTCTCATT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLFTVQWLAELFPKHQGKISTDITIQEVTRDTREPSSNSLYIPIVGERFDGHNFIFDAIKNGAIATLWDKSIPLPSEWDN
SFPVFFVEDTILAMQQLANSYLRSVNPTVVGVTGSNGKTTTKDLITSVVKTSYKTTATQGNLNNHIGLPLTILSMQPDTE
VLVLEMGMSQFGEIELLSKIAEPDYTVITNIGESHIENLGSRKGIAKAKLEIIEGIKSNGNLFIDDDEPLLAKENIDMDD
SQIIGIGFTKESDINISNVKVHPDRTTFEVTDKPYHVNLLGEHHAKNAAFAISIGKALNISHENIQLAFNKLEVTGMRFE
LETGINGVHIINDAYNASATSMKASIQVVKEMEGFQNKVLVLGDILELGTYSEHYHRSIAEVIDSRISEIYTYGEQAFYI
YDEITKQHPKVNVHYIKQREDVVPSLKEHLNNQTLILFKASRGMKFEMFIKELQQ

Sequences:

>Translated_455_residues
MLFTVQWLAELFPKHQGKISTDITIQEVTRDTREPSSNSLYIPIVGERFDGHNFIFDAIKNGAIATLWDKSIPLPSEWDN
SFPVFFVEDTILAMQQLANSYLRSVNPTVVGVTGSNGKTTTKDLITSVVKTSYKTTATQGNLNNHIGLPLTILSMQPDTE
VLVLEMGMSQFGEIELLSKIAEPDYTVITNIGESHIENLGSRKGIAKAKLEIIEGIKSNGNLFIDDDEPLLAKENIDMDD
SQIIGIGFTKESDINISNVKVHPDRTTFEVTDKPYHVNLLGEHHAKNAAFAISIGKALNISHENIQLAFNKLEVTGMRFE
LETGINGVHIINDAYNASATSMKASIQVVKEMEGFQNKVLVLGDILELGTYSEHYHRSIAEVIDSRISEIYTYGEQAFYI
YDEITKQHPKVNVHYIKQREDVVPSLKEHLNNQTLILFKASRGMKFEMFIKELQQ
>Mature_455_residues
MLFTVQWLAELFPKHQGKISTDITIQEVTRDTREPSSNSLYIPIVGERFDGHNFIFDAIKNGAIATLWDKSIPLPSEWDN
SFPVFFVEDTILAMQQLANSYLRSVNPTVVGVTGSNGKTTTKDLITSVVKTSYKTTATQGNLNNHIGLPLTILSMQPDTE
VLVLEMGMSQFGEIELLSKIAEPDYTVITNIGESHIENLGSRKGIAKAKLEIIEGIKSNGNLFIDDDEPLLAKENIDMDD
SQIIGIGFTKESDINISNVKVHPDRTTFEVTDKPYHVNLLGEHHAKNAAFAISIGKALNISHENIQLAFNKLEVTGMRFE
LETGINGVHIINDAYNASATSMKASIQVVKEMEGFQNKVLVLGDILELGTYSEHYHRSIAEVIDSRISEIYTYGEQAFYI
YDEITKQHPKVNVHYIKQREDVVPSLKEHLNNQTLILFKASRGMKFEMFIKELQQ

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=451, Percent_Identity=31.9290465631929, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51007; Mature: 51007

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFTVQWLAELFPKHQGKISTDITIQEVTRDTREPSSNSLYIPIVGERFDGHNFIFDAIK
CEEEHHHHHHHCCCCCCCEECCEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHC
NGAIATLWDKSIPLPSEWDNSFPVFFVEDTILAMQQLANSYLRSVNPTVVGVTGSNGKTT
CCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCH
TKDLITSVVKTSYKTTATQGNLNNHIGLPLTILSMQPDTEVLVLEMGMSQFGEIELLSKI
HHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHH
AEPDYTVITNIGESHIENLGSRKGIAKAKLEIIEGIKSNGNLFIDDDEPLLAKENIDMDD
CCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCC
SQIIGIGFTKESDINISNVKVHPDRTTFEVTDKPYHVNLLGEHHAKNAAFAISIGKALNI
CEEEEEEECCCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCEEEC
SHENIQLAFNKLEVTGMRFELETGINGVHIINDAYNASATSMKASIQVVKEMEGFQNKVL
CCCCEEEEEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEE
VLGDILELGTYSEHYHRSIAEVIDSRISEIYTYGEQAFYIYDEITKQHPKVNVHYIKQRE
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCEEEEEECCHH
DVVPSLKEHLNNQTLILFKASRGMKFEMFIKELQQ
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MLFTVQWLAELFPKHQGKISTDITIQEVTRDTREPSSNSLYIPIVGERFDGHNFIFDAIK
CEEEHHHHHHHCCCCCCCEECCEEHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHC
NGAIATLWDKSIPLPSEWDNSFPVFFVEDTILAMQQLANSYLRSVNPTVVGVTGSNGKTT
CCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCH
TKDLITSVVKTSYKTTATQGNLNNHIGLPLTILSMQPDTEVLVLEMGMSQFGEIELLSKI
HHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHH
AEPDYTVITNIGESHIENLGSRKGIAKAKLEIIEGIKSNGNLFIDDDEPLLAKENIDMDD
CCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCC
SQIIGIGFTKESDINISNVKVHPDRTTFEVTDKPYHVNLLGEHHAKNAAFAISIGKALNI
CEEEEEEECCCCCCCEEEEEECCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCEEEC
SHENIQLAFNKLEVTGMRFELETGINGVHIINDAYNASATSMKASIQVVKEMEGFQNKVL
CCCCEEEEEEEEEEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEE
VLGDILELGTYSEHYHRSIAEVIDSRISEIYTYGEQAFYIYDEITKQHPKVNVHYIKQRE
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCEEEEEECCHH
DVVPSLKEHLNNQTLILFKASRGMKFEMFIKELQQ
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]