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The map label for this gene is lutR [H]

Identifier: 23097824

GI number: 23097824

Start: 394239

End: 394949

Strand: Direct

Name: lutR [H]

Synonym: OB0369

Alternate gene names: 23097824

Gene position: 394239-394949 (Clockwise)

Preceding gene: 23097823

Following gene: 23097825

Centisome position: 10.86

GC content: 35.72

Gene sequence:

>711_bases
ATGGAGTACAAACGTATTAAATCCAAAAAAATATATGAACAAGTAGCAGATTCCTTAATAGATATGATTAAACAAGGGCG
TATACAGCCTGGAGATAAATTAGATTCTGTAGAACAATTGGCACAAAACTTCGGAGTAGGGAGATCGGCTATTCGTGAAG
CACTAAGTGGTTTAAGGTCTATGGGATTAGTAGAAATGAGACAAGGTGAAGGTACATTTATTAATACCTTTAATCCGAAG
AGATTTACACTACCTGTTAGTACAGCATTTCTGATGAAACAAGATGATGTAAAAGAATTATATCAAGTCCGTAAGATTCT
TGAAGGCGGTACGGCAAGATATGCTGCAACCAGCTATGAACAGGCAGATCTAGAAAAGATGGAAAAAACGTTAAACATAA
TGAAAAATGCTAAAGGGGATGATGTATTAGCGGAACAAGCGGATACGGAATTTCACTTAGCGATTGCCCAAGCGACACAT
ATGGATTTATTAATTCATTTAATGGGCAGTGTGTCTGATTTGATGTCAGAGACAATTCGAGAAACAAGACAAATATTATT
ATATTCAGAAGACCGAGCGGATGCGCTATATGATGAGCATGACCGTATATATCAGGCAATTAAAAATAGACAACCAAATG
AAGCGAGAGACGCAATGTACGATCATTTAGAAAAAGTAGAAATGCTCTTATTTAAAAATCTAGATATGTAA

Upstream 100 bases:

>100_bases
TGCTCATTTCTTTTTGTAGATTATCGTAGTCCTTGCCATCAGCTTTTCAAACTGGTTCGTTTCCATTAAAATGGTCTCAA
GTGATGAGGAGTTGAAATGA

Downstream 100 bases:

>100_bases
TTCTAGCCTCGGTCATAGTTCAAATTAGAAGAGGTGTAACCAAATGAAAGTATCGTTATTCATTACATGTGTGAGTGATA
TTGTATTTGCAGATGTAGGT

Product: transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEYKRIKSKKIYEQVADSLIDMIKQGRIQPGDKLDSVEQLAQNFGVGRSAIREALSGLRSMGLVEMRQGEGTFINTFNPK
RFTLPVSTAFLMKQDDVKELYQVRKILEGGTARYAATSYEQADLEKMEKTLNIMKNAKGDDVLAEQADTEFHLAIAQATH
MDLLIHLMGSVSDLMSETIRETRQILLYSEDRADALYDEHDRIYQAIKNRQPNEARDAMYDHLEKVEMLLFKNLDM

Sequences:

>Translated_236_residues
MEYKRIKSKKIYEQVADSLIDMIKQGRIQPGDKLDSVEQLAQNFGVGRSAIREALSGLRSMGLVEMRQGEGTFINTFNPK
RFTLPVSTAFLMKQDDVKELYQVRKILEGGTARYAATSYEQADLEKMEKTLNIMKNAKGDDVLAEQADTEFHLAIAQATH
MDLLIHLMGSVSDLMSETIRETRQILLYSEDRADALYDEHDRIYQAIKNRQPNEARDAMYDHLEKVEMLLFKNLDM
>Mature_236_residues
MEYKRIKSKKIYEQVADSLIDMIKQGRIQPGDKLDSVEQLAQNFGVGRSAIREALSGLRSMGLVEMRQGEGTFINTFNPK
RFTLPVSTAFLMKQDDVKELYQVRKILEGGTARYAATSYEQADLEKMEKTLNIMKNAKGDDVLAEQADTEFHLAIAQATH
MDLLIHLMGSVSDLMSETIRETRQILLYSEDRADALYDEHDRIYQAIKNRQPNEARDAMYDHLEKVEMLLFKNLDM

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789353, Length=231, Percent_Identity=33.7662337662338, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1786303, Length=234, Percent_Identity=30.3418803418803, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1790032, Length=225, Percent_Identity=30.6666666666667, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790780, Length=248, Percent_Identity=29.8387096774194, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082208, Length=226, Percent_Identity=27.8761061946903, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789621, Length=239, Percent_Identity=25.5230125523013, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994955, Length=200, Percent_Identity=25.5, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27075; Mature: 27075

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKRIKSKKIYEQVADSLIDMIKQGRIQPGDKLDSVEQLAQNFGVGRSAIREALSGLRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MGLVEMRQGEGTFINTFNPKRFTLPVSTAFLMKQDDVKELYQVRKILEGGTARYAATSYE
CCCHHEECCCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHH
QADLEKMEKTLNIMKNAKGDDVLAEQADTEFHLAIAQATHMDLLIHLMGSVSDLMSETIR
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETRQILLYSEDRADALYDEHDRIYQAIKNRQPNEARDAMYDHLEKVEMLLFKNLDM
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEYKRIKSKKIYEQVADSLIDMIKQGRIQPGDKLDSVEQLAQNFGVGRSAIREALSGLRS
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
MGLVEMRQGEGTFINTFNPKRFTLPVSTAFLMKQDDVKELYQVRKILEGGTARYAATSYE
CCCHHEECCCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHH
QADLEKMEKTLNIMKNAKGDDVLAEQADTEFHLAIAQATHMDLLIHLMGSVSDLMSETIR
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETRQILLYSEDRADALYDEHDRIYQAIKNRQPNEARDAMYDHLEKVEMLLFKNLDM
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]