Click here to switch to the map view.

The map label for this gene is pemA [H]

Identifier: 22127628

GI number: 22127628

Start: 4175692

End: 4176777

Strand: Reverse

Name: pemA [H]

Synonym: y3757

Alternate gene names: 22127628

Gene position: 4176777-4175692 (Counterclockwise)

Preceding gene: 22127630

Following gene: 22127626

Centisome position: 90.78

GC content: 47.42

Gene sequence:

>1086_bases
ATGCCAATTAATGCTCTGAGTAAGACACTATTATTAGGATTGATATCATTTGCCGCTTTAGGTGAAGTCAGCGCGGCACA
GTACAATGCTGTGGTTTCTGCCATGGCGCAGGGCGATGAGTTCTCTTCAATTAATGCAGCGTTACAATCAGCACCGCCAG
ATAGTTCTCCGTTTGTTATTTTCCTTAAAAAAGGCGTTTATACCGAACGGCTTGAGGTCAATCGTGACAATGTGACGCTG
AAAGGTGAGGAGCGTGATACGACGGTGATTGGCGCGAATACCGCCGCGGGTATGCTCAACCCACAAGGGACGAAGTGGGG
AACCTCTGGCAGCAGCACGGTATTGGTGAATGCCGCCAATTTTACTGCAGAAAATTTGACGATTCGTAATGACTTCGATT
TCCCTGCTAATCAGGCGAAAGCAGAGGGTGATCCGACGAAATTAAAAGATACGCAGGCGGTGGCGCTGTTATTAGCAGAA
AAAAGTGATAAAGCCCGTTTCAGGCAAGTCAAACTTGAGGGGTACCAAGATACGCTTTATAGCAAAACGGGCAGTCGTAG
CTACTTTACCGATTGTGACATCAGTGGCCACGTCGATTTTATTTTTGGCTCAGGGGTGACGGTATTTGACCGCTGCAATA
TTATTGCCCGTGATCGCCGTGATATCTCCCCACCTTACGGTTACATTACGGCACCCAGTACCCAAAATACCGCACCTTAT
GGTCTGATTGTCCTCAATAGTCGTTTAACCAAAGAACCAGGTGTGCCTGCTAAAAGTTTTGCCTTAGGGCGCCCGTGGCA
CCCTACTACAGCATTTGATGATGGCCGTTATGCTGATCCTGCGGCTATCGGACAGGCGGTATTTATCAATACCGTAATGG
ATGATCATATTTATGGCTGGGATAAAATGTCTGGAAAAGATAAGCAAGGCGAGAAAGTTTGGTTCTACCCGCAAGACTCT
CGCTTCTTTGAAGCGAACAATCAGGGACCAGGGGCTGAAATTAATGAGGGGCGGCGTCAGTTGTCTGCTGAGCAACTAAA
AGCGTTCACATTGCCAATGATTTTTCCAGACTGGACCGTTCAGTAA

Upstream 100 bases:

>100_bases
TTAACGTGCTTTTTATCAACAGCTAGACGTGATTTTTATCAATGGCTAATAGCGAACAATCGAGTTTAATCTGCTGGATG
TTAAAAACTGAGGGTAATTC

Downstream 100 bases:

>100_bases
CCTCATATTAACCGCGGTTTACTGCCTTTAAGCTGGCGGATAATCCAAGAGGAGGCGTTTCACGTTTTACTTCTCATCAT
CATCTGATGCGGCCTATACC

Product: pectinesterase

Products: NA

Alternate protein names: PE A; Pectin methylesterase A [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MPINALSKTLLLGLISFAALGEVSAAQYNAVVSAMAQGDEFSSINAALQSAPPDSSPFVIFLKKGVYTERLEVNRDNVTL
KGEERDTTVIGANTAAGMLNPQGTKWGTSGSSTVLVNAANFTAENLTIRNDFDFPANQAKAEGDPTKLKDTQAVALLLAE
KSDKARFRQVKLEGYQDTLYSKTGSRSYFTDCDISGHVDFIFGSGVTVFDRCNIIARDRRDISPPYGYITAPSTQNTAPY
GLIVLNSRLTKEPGVPAKSFALGRPWHPTTAFDDGRYADPAAIGQAVFINTVMDDHIYGWDKMSGKDKQGEKVWFYPQDS
RFFEANNQGPGAEINEGRRQLSAEQLKAFTLPMIFPDWTVQ

Sequences:

>Translated_361_residues
MPINALSKTLLLGLISFAALGEVSAAQYNAVVSAMAQGDEFSSINAALQSAPPDSSPFVIFLKKGVYTERLEVNRDNVTL
KGEERDTTVIGANTAAGMLNPQGTKWGTSGSSTVLVNAANFTAENLTIRNDFDFPANQAKAEGDPTKLKDTQAVALLLAE
KSDKARFRQVKLEGYQDTLYSKTGSRSYFTDCDISGHVDFIFGSGVTVFDRCNIIARDRRDISPPYGYITAPSTQNTAPY
GLIVLNSRLTKEPGVPAKSFALGRPWHPTTAFDDGRYADPAAIGQAVFINTVMDDHIYGWDKMSGKDKQGEKVWFYPQDS
RFFEANNQGPGAEINEGRRQLSAEQLKAFTLPMIFPDWTVQ
>Mature_360_residues
PINALSKTLLLGLISFAALGEVSAAQYNAVVSAMAQGDEFSSINAALQSAPPDSSPFVIFLKKGVYTERLEVNRDNVTLK
GEERDTTVIGANTAAGMLNPQGTKWGTSGSSTVLVNAANFTAENLTIRNDFDFPANQAKAEGDPTKLKDTQAVALLLAEK
SDKARFRQVKLEGYQDTLYSKTGSRSYFTDCDISGHVDFIFGSGVTVFDRCNIIARDRRDISPPYGYITAPSTQNTAPYG
LIVLNSRLTKEPGVPAKSFALGRPWHPTTAFDDGRYADPAAIGQAVFINTVMDDHIYGWDKMSGKDKQGEKVWFYPQDSR
FFEANNQGPGAEINEGRRQLSAEQLKAFTLPMIFPDWTVQ

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 39452; Mature: 39320

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINALSKTLLLGLISFAALGEVSAAQYNAVVSAMAQGDEFSSINAALQSAPPDSSPFVI
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEE
FLKKGVYTERLEVNRDNVTLKGEERDTTVIGANTAAGMLNPQGTKWGTSGSSTVLVNAAN
EEECCCEEEEEEECCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECC
FTAENLTIRNDFDFPANQAKAEGDPTKLKDTQAVALLLAEKSDKARFRQVKLEGYQDTLY
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCEEEEEEECCCHHHHH
SKTGSRSYFTDCDISGHVDFIFGSGVTVFDRCNIIARDRRDISPPYGYITAPSTQNTAPY
HCCCCCCEEECCCCCCCEEEEEECCEEEEECCCEEEECCCCCCCCCCEEECCCCCCCCCE
GLIVLNSRLTKEPGVPAKSFALGRPWHPTTAFDDGRYADPAAIGQAVFINTVMDDHIYGW
EEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEHHHCCCCCC
DKMSGKDKQGEKVWFYPQDSRFFEANNQGPGAEINEGRRQLSAEQLKAFTLPMIFPDWTV
CCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHEECCEECCCCCC
Q
C
>Mature Secondary Structure 
PINALSKTLLLGLISFAALGEVSAAQYNAVVSAMAQGDEFSSINAALQSAPPDSSPFVI
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEE
FLKKGVYTERLEVNRDNVTLKGEERDTTVIGANTAAGMLNPQGTKWGTSGSSTVLVNAAN
EEECCCEEEEEEECCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECC
FTAENLTIRNDFDFPANQAKAEGDPTKLKDTQAVALLLAEKSDKARFRQVKLEGYQDTLY
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHEEEEEEECCCCCCEEEEEEECCCHHHHH
SKTGSRSYFTDCDISGHVDFIFGSGVTVFDRCNIIARDRRDISPPYGYITAPSTQNTAPY
HCCCCCCEEECCCCCCCEEEEEECCEEEEECCCEEEECCCCCCCCCCEEECCCCCCCCCE
GLIVLNSRLTKEPGVPAKSFALGRPWHPTTAFDDGRYADPAAIGQAVFINTVMDDHIYGW
EEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEEHHHCCCCCC
DKMSGKDKQGEKVWFYPQDSRFFEANNQGPGAEINEGRRQLSAEQLKAFTLPMIFPDWTV
CCCCCCCCCCCEEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHEECCEECCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370537 [H]