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The map label for this gene is rlmG [H]

Identifier: 22127464

GI number: 22127464

Start: 3983335

End: 3984525

Strand: Reverse

Name: rlmG [H]

Synonym: y3590

Alternate gene names: 22127464

Gene position: 3984525-3983335 (Counterclockwise)

Preceding gene: 22127471

Following gene: 22127454

Centisome position: 86.61

GC content: 49.62

Gene sequence:

>1191_bases
GTGATGAGCCAACTCGATCTGGGAACGCAAAGCCTTGAGCTTGAGCGTTTTCCTCCACAAGAAAATTCCAATACCCTTCA
GGCGTGGGAAGCGGCAGATGAATATCTGCTACAAAACATTGATTTAAGCCAAATTGATGGCCGCCCGGTGCTGGTGTTTA
ACGATCAGTTTGGTACGCTGGCCTGCGCTTTACACGCTTATCGCCCGTTCAGTTCCAGTGACTCTTATATGAGTCAGTTG
GCGACGGCGCATAATTTGCGTCTAAACCATTTAGATGAATCTGCGGTGACATTACTGAGCAGCGTCGATGACTTACCCGA
GGCACCTAAGCTGGTGGTCATTAAGATCCCGAAAGCCTTAGCGCTACTCGAACATCAATTACGTGCTTTGCGTCGCGTTG
TGGCACCAGATACGGTGATCATTGCCGGTGCAAAATCCCGCGATGTGCATAACTCCACGCTGCAACTGTTTGAAAAGATC
CTGGGGCCAACCAAAACGACGCTGGCTTGGAAAAAAGCGCGGCTGATTCACTGCGAGGTTGCTGATATTCCATTGGCTGA
CGCGCCGGAAACCATCGATTGGCCGCTGCCTAACACGGACTACATTATCCATAATCACGCGAACGTTTTCTCTCGCAATA
ATCTGGATATTGGCGCGCGTTTCTTTATGGAAATCTTGCCTTATGATGTCACCGGCAAAATTGCGGATTTAGGTTGTGGG
AATGGTGTCGTCGGGTTAATCGCACTGGAGCAAAACCCGCTAGCTGAAATGCTGTTTGTTGATGAATCCTATATGGCAGT
GGCATCCAGTGAATTGAACATCACGGTTAACCGCCCGCAAGACTTGTCGCGCTGTGAATTTATGGTGAGCCATGGTTTGG
CGGGTGTGGAGCGTGAAAGCCTGCAATTGGTGTTATGTAATCCACCGTTCCACCAACAACATGCGGTCAGTGACCATGTC
GCCTGGCAGATGTTCTGTGATGCCAAGCGTTGTCTGAAAGCGGGTGGCGAGTTGATGATCGTGGGTAACCGCCATCTGGA
TTACTTCCATAAGCTCAAGCGTTTGTTTGGCAACTGCGAAACACTGGATTCAAACCAGAAATTTATGGTGCTGAAATCGG
TTAAGCAGGCCTCCTCTCGCTCAGAAGGCGGCGGTTCCGGTAGTCTCGATATGTCATATAGCGATTTCTAA

Upstream 100 bases:

>100_bases
CGCTTGGCACAAGCGCGTTTAAAACGCATTAAAGCAAAAAAACATTTTACTGATCGCCCGTTTTAGGGGATAAACAGCCC
AAATTTATTATTCAGGAGCG

Downstream 100 bases:

>100_bases
TCGGTATTTCACAGGGTGGGGTTTTCGCCACACCCTGTGATTATTCTCAGTTCTATTGCTATTCTCGGCCTTGTTACGGC
CCTTTACCTTGCTGGTCGCG

Product: enzyme

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MMSQLDLGTQSLELERFPPQENSNTLQAWEAADEYLLQNIDLSQIDGRPVLVFNDQFGTLACALHAYRPFSSSDSYMSQL
ATAHNLRLNHLDESAVTLLSSVDDLPEAPKLVVIKIPKALALLEHQLRALRRVVAPDTVIIAGAKSRDVHNSTLQLFEKI
LGPTKTTLAWKKARLIHCEVADIPLADAPETIDWPLPNTDYIIHNHANVFSRNNLDIGARFFMEILPYDVTGKIADLGCG
NGVVGLIALEQNPLAEMLFVDESYMAVASSELNITVNRPQDLSRCEFMVSHGLAGVERESLQLVLCNPPFHQQHAVSDHV
AWQMFCDAKRCLKAGGELMIVGNRHLDYFHKLKRLFGNCETLDSNQKFMVLKSVKQASSRSEGGGSGSLDMSYSDF

Sequences:

>Translated_396_residues
MMSQLDLGTQSLELERFPPQENSNTLQAWEAADEYLLQNIDLSQIDGRPVLVFNDQFGTLACALHAYRPFSSSDSYMSQL
ATAHNLRLNHLDESAVTLLSSVDDLPEAPKLVVIKIPKALALLEHQLRALRRVVAPDTVIIAGAKSRDVHNSTLQLFEKI
LGPTKTTLAWKKARLIHCEVADIPLADAPETIDWPLPNTDYIIHNHANVFSRNNLDIGARFFMEILPYDVTGKIADLGCG
NGVVGLIALEQNPLAEMLFVDESYMAVASSELNITVNRPQDLSRCEFMVSHGLAGVERESLQLVLCNPPFHQQHAVSDHV
AWQMFCDAKRCLKAGGELMIVGNRHLDYFHKLKRLFGNCETLDSNQKFMVLKSVKQASSRSEGGGSGSLDMSYSDF
>Mature_396_residues
MMSQLDLGTQSLELERFPPQENSNTLQAWEAADEYLLQNIDLSQIDGRPVLVFNDQFGTLACALHAYRPFSSSDSYMSQL
ATAHNLRLNHLDESAVTLLSSVDDLPEAPKLVVIKIPKALALLEHQLRALRRVVAPDTVIIAGAKSRDVHNSTLQLFEKI
LGPTKTTLAWKKARLIHCEVADIPLADAPETIDWPLPNTDYIIHNHANVFSRNNLDIGARFFMEILPYDVTGKIADLGCG
NGVVGLIALEQNPLAEMLFVDESYMAVASSELNITVNRPQDLSRCEFMVSHGLAGVERESLQLVLCNPPFHQQHAVSDHV
AWQMFCDAKRCLKAGGELMIVGNRHLDYFHKLKRLFGNCETLDSNQKFMVLKSVKQASSRSEGGGSGSLDMSYSDF

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA [H]

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family [H]

Homologues:

Organism=Escherichia coli, GI87082206, Length=379, Percent_Identity=63.0606860158311, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1790830, Length=284, Percent_Identity=25.7042253521127, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.174 [H]

Molecular weight: Translated: 43973; Mature: 43973

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSQLDLGTQSLELERFPPQENSNTLQAWEAADEYLLQNIDLSQIDGRPVLVFNDQFGTL
CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCHH
ACALHAYRPFSSSDSYMSQLATAHNLRLNHLDESAVTLLSSVDDLPEAPKLVVIKIPKAL
HHHHHHCCCCCCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCCCCCEEEEECCHHH
ALLEHQLRALRRVVAPDTVIIAGAKSRDVHNSTLQLFEKILGPTKTTLAWKKARLIHCEV
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHCEEEEEEEE
ADIPLADAPETIDWPLPNTDYIIHNHANVFSRNNLDIGARFFMEILPYDVTGKIADLGCG
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCCCCCEEECCCC
NGVVGLIALEQNPLAEMLFVDESYMAVASSELNITVNRPQDLSRCEFMVSHGLAGVERES
CCEEEEEEECCCCCCCEEEECCCHHEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
LQLVLCNPPFHQQHAVSDHVAWQMFCDAKRCLKAGGELMIVGNRHLDYFHKLKRLFGNCE
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCC
TLDSNQKFMVLKSVKQASSRSEGGGSGSLDMSYSDF
CCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMSQLDLGTQSLELERFPPQENSNTLQAWEAADEYLLQNIDLSQIDGRPVLVFNDQFGTL
CCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCHH
ACALHAYRPFSSSDSYMSQLATAHNLRLNHLDESAVTLLSSVDDLPEAPKLVVIKIPKAL
HHHHHHCCCCCCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCCCCCEEEEECCHHH
ALLEHQLRALRRVVAPDTVIIAGAKSRDVHNSTLQLFEKILGPTKTTLAWKKARLIHCEV
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHCEEEEEEEE
ADIPLADAPETIDWPLPNTDYIIHNHANVFSRNNLDIGARFFMEILPYDVTGKIADLGCG
CCCCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHCCCCCCCCEEECCCC
NGVVGLIALEQNPLAEMLFVDESYMAVASSELNITVNRPQDLSRCEFMVSHGLAGVERES
CCEEEEEEECCCCCCCEEEECCCHHEEECCCEEEEEECCCCHHHHHHHHHHCCCCCCCCC
LQLVLCNPPFHQQHAVSDHVAWQMFCDAKRCLKAGGELMIVGNRHLDYFHKLKRLFGNCE
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCC
TLDSNQKFMVLKSVKQASSRSEGGGSGSLDMSYSDF
CCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA