Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 22127403

GI number: 22127403

Start: 3913843

End: 3915081

Strand: Direct

Name: cca [H]

Synonym: y3529

Alternate gene names: 22127403

Gene position: 3913843-3915081 (Clockwise)

Preceding gene: 22127402

Following gene: 229013124

Centisome position: 85.07

GC content: 53.35

Gene sequence:

>1239_bases
ATGAACATCTACCTGGTCGGCGGAGCCGTACGCGATAGCTTATTAAACCTGCCCGTAACCGAACAGGACTGGGTCGTGGT
AGGGGCGACCCCTGAACAATTACTGAAATTAGGGTATCAGCAGGTCGGTAAAGACTTTCCGGTCTTCCTGCACCCCGTCA
GCCATGAAGAATACGCCCTAGCGCGTACTGAACGTAAATCCGGCCAGGGCTACACCGGTTTTACCTGCTATGCCGCTCCC
GATGTCACGCTGGAAGATGATTTATTACGCCGCGATTTAACCGTTAATGCCATTGCCCGTAGTGCTGACGGGGAATTTAT
CGATCCCTACCATGGCAAACAGGACTTGGAAAACCGAGTGTTACGCCATGTCTCCGACGCATTTGGTGAGGATCCATTGC
GCGTTCTGCGGGTGGCACGCTTTGCCGCCCGTTTTGCCTACCTTGGTTTCACCATCGCCCCAGAAACGATGTCTCTGATG
AGCAATATGGCGCAGAGTGGCGAACTCTCCGCCCTAACACCAGAGCGCGTGTGGAAAGAAACCGAGAAAGCACTGAAAAC
GCAAAGCCCACACGTCTATTTTCAGGTACTTCGGGATTGCGGTGCGTTGGCGGTGCTGTTCCCTGAGATTGAGCGTTTAT
TTGGGGTGCCGGCACCGGAGAAATGGCACCCGGAAATTGATACCGGTATCCATACTCTGATGACGTTGGCTATCGCTGCA
CAGCTCAGCCCGGAGGTTGATATACGCTTTGCTGCCCTGTGCCACGATTTGGGGAAAGGCCTAACCCCCAAAGAGCATTG
GCCACATCATCACGGCCATGGTCCGGCTGGCGTCAAATTAGTTGAGCAACTGTGCCAACGGTTGCGGATCCCTAACCCTG
TGCGTGATTTGGCTAAGCTCGTCGCGGAATACCATGACCTAATTCATACAGTGAATAAACTCCGGCCAGAAACTCTGCTA
AAACTGTTTAATGCCATTGATGTATGGCGTAAACCTGAGCGCCTGGAGCAGATGATTATGACCAGCGAAGCGGATGCCCG
TGGCCGGACTGGATTTGAAAACAATCCCTATCCACAAGGGGATTATCTGCGGGCCGCCTTCCAGATTGCCAACGGAGTAT
CAATACAGGAAGTGGTCGCCAGCGGGTTACAAGGCTTGGCTATTCGCGACGAACTTCAACGCCGCCGCCAGCAAGCTCTG
GCCGAGTGGAAACAGACTCAGGAAACAGCTTCAATATAA

Upstream 100 bases:

>100_bases
ACAAGTCAGTGACTCGGGTTATTGAGTGTAGTCAACGCACATGCAGCTTGAAGTATGACGGGTATAACCACGCTGTGGCG
TCAAGAACAAAGGGTTAACT

Downstream 100 bases:

>100_bases
GTGACTCCCTGTTCGTTTCCCCCTGTAAAACAAGGGGTTTCAGACTCCTGCCCCCCCGATGACACGATCTTGAACGGTGA
GGATGGGCAGAAGAGTAAAG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNIYLVGGAVRDSLLNLPVTEQDWVVVGATPEQLLKLGYQQVGKDFPVFLHPVSHEEYALARTERKSGQGYTGFTCYAAP
DVTLEDDLLRRDLTVNAIARSADGEFIDPYHGKQDLENRVLRHVSDAFGEDPLRVLRVARFAARFAYLGFTIAPETMSLM
SNMAQSGELSALTPERVWKETEKALKTQSPHVYFQVLRDCGALAVLFPEIERLFGVPAPEKWHPEIDTGIHTLMTLAIAA
QLSPEVDIRFAALCHDLGKGLTPKEHWPHHHGHGPAGVKLVEQLCQRLRIPNPVRDLAKLVAEYHDLIHTVNKLRPETLL
KLFNAIDVWRKPERLEQMIMTSEADARGRTGFENNPYPQGDYLRAAFQIANGVSIQEVVASGLQGLAIRDELQRRRQQAL
AEWKQTQETASI

Sequences:

>Translated_412_residues
MNIYLVGGAVRDSLLNLPVTEQDWVVVGATPEQLLKLGYQQVGKDFPVFLHPVSHEEYALARTERKSGQGYTGFTCYAAP
DVTLEDDLLRRDLTVNAIARSADGEFIDPYHGKQDLENRVLRHVSDAFGEDPLRVLRVARFAARFAYLGFTIAPETMSLM
SNMAQSGELSALTPERVWKETEKALKTQSPHVYFQVLRDCGALAVLFPEIERLFGVPAPEKWHPEIDTGIHTLMTLAIAA
QLSPEVDIRFAALCHDLGKGLTPKEHWPHHHGHGPAGVKLVEQLCQRLRIPNPVRDLAKLVAEYHDLIHTVNKLRPETLL
KLFNAIDVWRKPERLEQMIMTSEADARGRTGFENNPYPQGDYLRAAFQIANGVSIQEVVASGLQGLAIRDELQRRRQQAL
AEWKQTQETASI
>Mature_412_residues
MNIYLVGGAVRDSLLNLPVTEQDWVVVGATPEQLLKLGYQQVGKDFPVFLHPVSHEEYALARTERKSGQGYTGFTCYAAP
DVTLEDDLLRRDLTVNAIARSADGEFIDPYHGKQDLENRVLRHVSDAFGEDPLRVLRVARFAARFAYLGFTIAPETMSLM
SNMAQSGELSALTPERVWKETEKALKTQSPHVYFQVLRDCGALAVLFPEIERLFGVPAPEKWHPEIDTGIHTLMTLAIAA
QLSPEVDIRFAALCHDLGKGLTPKEHWPHHHGHGPAGVKLVEQLCQRLRIPNPVRDLAKLVAEYHDLIHTVNKLRPETLL
KLFNAIDVWRKPERLEQMIMTSEADARGRTGFENNPYPQGDYLRAAFQIANGVSIQEVVASGLQGLAIRDELQRRRQQAL
AEWKQTQETASI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=405, Percent_Identity=72.5925925925926, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI87081691, Length=325, Percent_Identity=28.6153846153846, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=160, Percent_Identity=35.625, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46289; Mature: 46289

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYLVGGAVRDSLLNLPVTEQDWVVVGATPEQLLKLGYQQVGKDFPVFLHPVSHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
ARTERKSGQGYTGFTCYAAPDVTLEDDLLRRDLTVNAIARSADGEFIDPYHGKQDLENRV
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHH
LRHVSDAFGEDPLRVLRVARFAARFAYLGFTIAPETMSLMSNMAQSGELSALTPERVWKE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHCCCCCCCCHHHHHHH
TEKALKTQSPHVYFQVLRDCGALAVLFPEIERLFGVPAPEKWHPEIDTGIHTLMTLAIAA
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QLSPEVDIRFAALCHDLGKGLTPKEHWPHHHGHGPAGVKLVEQLCQRLRIPNPVRDLAKL
HCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
VAEYHDLIHTVNKLRPETLLKLFNAIDVWRKPERLEQMIMTSEADARGRTGFENNPYPQG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
DYLRAAFQIANGVSIQEVVASGLQGLAIRDELQRRRQQALAEWKQTQETASI
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIYLVGGAVRDSLLNLPVTEQDWVVVGATPEQLLKLGYQQVGKDFPVFLHPVSHEEYAL
CEEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCHHHH
ARTERKSGQGYTGFTCYAAPDVTLEDDLLRRDLTVNAIARSADGEFIDPYHGKQDLENRV
HHHHHHCCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHH
LRHVSDAFGEDPLRVLRVARFAARFAYLGFTIAPETMSLMSNMAQSGELSALTPERVWKE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHCCCCCCCCHHHHHHH
TEKALKTQSPHVYFQVLRDCGALAVLFPEIERLFGVPAPEKWHPEIDTGIHTLMTLAIAA
HHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
QLSPEVDIRFAALCHDLGKGLTPKEHWPHHHGHGPAGVKLVEQLCQRLRIPNPVRDLAKL
HCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
VAEYHDLIHTVNKLRPETLLKLFNAIDVWRKPERLEQMIMTSEADARGRTGFENNPYPQG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
DYLRAAFQIANGVSIQEVVASGLQGLAIRDELQRRRQQALAEWKQTQETASI
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA