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The map label for this gene is citF [H]

Identifier: 21910370

GI number: 21910370

Start: 886584

End: 888116

Strand: Direct

Name: citF [H]

Synonym: SpyM3_0834

Alternate gene names: 21910370

Gene position: 886584-888116 (Clockwise)

Preceding gene: 21910369

Following gene: 21910371

Centisome position: 46.65

GC content: 42.79

Gene sequence:

>1533_bases
ATGGTAACAAATAAACTCGGTAGAGATATTCCTCAACCATACGCCGACCAATACGGTGTCTTTGAAGGAGAACTCGCAAA
TATCAAACAGTATGACGAATCAAGTCGCCGTATTAAACCAGTTAAGCCTGGAGATTCTAAATTACTAGGATCTGTTCGTG
AAGCGATTGAAAAAACAGGTTTAACAGATGGCATGACCATTTCTTTCCACCACCATTTCCGTGAAGGGGATTTCATTATG
AATATGGTCTTGGAGGAAATTGCCAAAATGGGCATTAAAAACCTGTCTATTGCCCCAAGTTCCATCGCTAATGTACATGA
GCCTTTGATTGATCACATCAAAAATGGTGTGGTCACTAACATCACTTCATCTGGTCTTCGTGACAAAGTAGGGGCAGCCA
TCTCAGAAGGACTAATGGAAAATCCTGTGGTTATTCGCTCCCATGGTGGTCGCGCTCGTGCCATTGCTAGTGGGGATATC
CATATTGATGTTGCCTTTCTGGGCGCCCCAAGTTCAGATGCTTACGGAAATGTTAATGGGACAAAAGGGAAAGCAACCTG
TGGTTCTTTGGGCTATGCCATGATTGATGCCAAATATGCGGATCAGGTGGTTATCTTGACAGATAATTTGGTTCCTTATC
CTAATACCCCAATCAGCATTCCTCAAACAGATGTTGACTATGTGGTAACAGTGGATGCTATCGGAGATCCTCAAGGAATC
GCCAAAGGGGCAACTCGTTTTACGAAAAATCCTAAAGAACTCTTAATTGCAGAATACGCCGCTAAAGTAATCACCAACTC
TCCTTATTTTAAAGAAGGATTCTCTTTCCAAACTGGAACAGGTGGCGCTTCTTTGGCAGTAACCCGTTTCATGCGTGAAG
CTATGATTAAAGAAAATATCAAAGCTAGCTTTGCTCTTGGTGGTATTACCAATGCTATGGTGGAGTTGCTCGAAGAAGGA
CTGGTTGAAAAAATTCTTGATGTCCAAGACTTTGATCACCCATCAGCTGTTTCTCTTGGTAAGCATGCTGACCATTATGA
AATTGATGCCAACATGTATGCCTCACCTTTGAGCAAGGGTGCTGTTATCAATCAATTAGACACTTGTATTTTATCAGCCC
TTGAAGTCGATACTAACTTCAATGTTAATGTGATGACAGGATCTGACGGCGTGATTCGTGGCGCTTCTGGAGGACACTGC
GACACTGCCTTTGCGGCTAAGATGAGTTTGGTTATTTCACCACTTATCCGCGGACGCATCCCAACTTTTGTAGATGAGGT
TAATACGGTCATTACACCAGGAACAAGTGTCGATGTGGTCGTCACAGAAGTGGGAATTGCCATTAACCCAAATCGTCAAG
ACTTAGTAGACCATTTCAAATCCCTCAATGTGCCACAATTTAGTATTGAGGAGCTAAAAGAGAAGGCTTACGCTATCGTT
GGCACACCTGAGCGTATTCAATATGGTGATAAGGTTGTTGCTCTTATTGAATACCGTGATGGTAGCCTCATGGATGTGGT
TTACAATGTGTAA

Upstream 100 bases:

>100_bases
TAAATGGAAAAATGGTTGATAAACCAATCGTTGAACGTGCAGAACGTGTCATTGCCCTAGCAACAGCAGCAGGTGTTTTA
TCTGAGGAGGACATTTAAAG

Downstream 100 bases:

>100_bases
AGACACTTATTTTTCAGGCGAAGCTATCCAACTTAGTGATATGTTAAGAGCCCGCGAAGAAAGAGCTCTGCGTCAGCTGC
ATTTATTAAAGGAGTACCCA

Product: putative citrate lyase alpha subunit

Products: NA

Alternate protein names: Citrase alpha chain; Citrate (pro-3S)-lyase alpha chain; Citrate CoA-transferase subunit [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIM
NMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEG
LVEKILDVQDFDHPSAVSLGKHADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
GTPERIQYGDKVVALIEYRDGSLMDVVYNV

Sequences:

>Translated_510_residues
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIM
NMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEG
LVEKILDVQDFDHPSAVSLGKHADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
GTPERIQYGDKVVALIEYRDGSLMDVVYNV
>Mature_510_residues
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIM
NMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEG
LVEKILDVQDFDHPSAVSLGKHADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHC
DTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
GTPERIQYGDKVVALIEYRDGSLMDVVYNV

Specific function: Represents a citrate:acetyl-ACP transferase [H]

COG id: COG3051

COG function: function code C; Citrate lyase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786832, Length=469, Percent_Identity=57.5692963752665, Blast_Score=570, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006472 [H]

Pfam domain/function: PF04223 CitF [H]

EC number: =4.1.3.6; =2.8.3.10 [H]

Molecular weight: Translated: 54891; Mature: 54891

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTG
CCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
LTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTN
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEE
ITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDIHIDVAFLGAPSSDAYGNVNG
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
TKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
CCCCCCCCCCCCCEEEHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHH
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENI
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
KASFALGGITNAMVELLEEGLVEKILDVQDFDHPSAVSLGKHADHYEIDANMYASPLSKG
HHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEECCCHHHCCCCCC
AVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHCDTAFAAKMSLVISPLIRGRI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
PTFVDEVNTVITPGTSVDVVVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
CHHHHCCCCEECCCCCEEEEEEEECEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEE
GTPERIQYGDKVVALIEYRDGSLMDVVYNV
CCHHHHCCCCEEEEEEEECCCCEEEHEECC
>Mature Secondary Structure
MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPGDSKLLGSVREAIEKTG
CCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
LTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSIANVHEPLIDHIKNGVVTN
CCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEE
ITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDIHIDVAFLGAPSSDAYGNVNG
CCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCCEEEEEEEEECCCCCCCCCCCC
TKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPISIPQTDVDYVVTVDAIGDPQGI
CCCCCCCCCCCCCEEEHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHH
AKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSFQTGTGGASLAVTRFMREAMIKENI
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
KASFALGGITNAMVELLEEGLVEKILDVQDFDHPSAVSLGKHADHYEIDANMYASPLSKG
HHEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEECCCHHHCCCCCC
AVINQLDTCILSALEVDTNFNVNVMTGSDGVIRGASGGHCDTAFAAKMSLVISPLIRGRI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
PTFVDEVNTVITPGTSVDVVVTEVGIAINPNRQDLVDHFKSLNVPQFSIEELKEKAYAIV
CHHHHCCCCEECCCCCEEEEEEEECEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEE
GTPERIQYGDKVVALIEYRDGSLMDVVYNV
CCHHHHCCCCEEEEEEEECCCCEEEHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]