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The map label for this gene is gid [H]

Identifier: 21910357

GI number: 21910357

Start: 874665

End: 876149

Strand: Direct

Name: gid [H]

Synonym: SpyM3_0821

Alternate gene names: 21910357

Gene position: 874665-876149 (Clockwise)

Preceding gene: 21910356

Following gene: 161485745

Centisome position: 46.02

GC content: 42.96

Gene sequence:

>1485_bases
ATGAGGATTACAAAAGAGGCTACACATAGCCTCTTTTGCTTTTTTCCCCTTTCTGTTATAATAAGTCAGGTAACTACATT
AGAAAGATTTGTGGGTCTGTCATCTAAGAGATGTCATTCCCGAAGAGGTATTAGTGACTTGTCTCAATCAACTGCAACTT
ATATTAATGTTATTGGAGCTGGACTAGCTGGTTCTGAAGCTGCCTATCAGATTGCTAAGCGCGGTATCCCCGTTAAATTG
TATGAAATGCGTGGTGTCAAAGCAACACCGCAACATAAAACCACTAATTTTGCCGAATTGGTCTGTTCCAACTCATTTCG
TGGTGATAGCTTAACCAATGCAGTCGGTCTTCTCAAAGAAGAAATGCGTCGCTTGGATTCCATTATTATGCGTAATGGTG
AAGCTAATCGTGTACCTGCTGGAGGAGCAATGGCTGTTGACCGTGAGGGGTATGCAAAGAGTGTCACTGCAGAGTTGGAA
AATCATCCTCTCATTGAGGTCATTCGTGATGAAATCACAGAAATTCCTAACGATGCCATTACGGTTATCGCGACGGGACC
GCTGACTTCGGATGCCCTGGCAGAAAAAATTCACGCGGTAAATGGTGGCGATGGATTCTATTTCTACGACGCAGCAGCGC
CTATCATTGATAAATCTACTATTGATATGAGCAAGGTTTACCTCAAATCTCGCTACGATAAAGGCGAAGCTGCTTACCTC
AACTGCCCTATGACCAAAGAAGAATTCATGGCTTTCCATGAAGCTCTGACAACCGCAGAAGAAGCCCCGCTGAATTCTTT
TGAAAAAGAAAAGTATTTTGAAGGCTGTATGCCGATTGAAGTTATGGCTAAACGTGGCATTAAAACCATGCTTTATGGAC
CTATGAAACCCGTTGGATTGGAATATCCAGATGACTATACAGGTCCTCGCGATGGAGAATTTAAAACGCCATATGCCGTC
GTGCAATTGCGTCAAGATAATGCAGCTGGAAGCCTTTATAATATCGTTGGTTTCCAAACCCATCTCAAATGGGGTGAGCA
AAAACGCGTTTTCCAAATGATTCCAGGGCTTGAAAATGCTGAGTTTGTCCGCTACGGCGTCATGCATCGCAATTCCTATA
TGGATTCACCAAATCTTTTAACGGAAACCTTCCAATCTCGGAGCAATCCAAACCTTTTATTTGCAGGTCAGATGACTGGA
GTTGAAGGTTATGTCGAATCAGCTGCTTCAGGTTTAGTAGCAGGAATCAATGCTGCTCGTTTGTTCAAAAGAGAAGAAGC
ACTTATTTTTCCTCAGACAACAGCTATTGGGAGTTTGCCTCATTATGTGACTCATGCCGACAGTAAGCATTTCCAACCAA
TGAACGTCAACTTTGGCATCATCAAAGAGTTAGAAGGCCCACGCATTCGTGACAAAAAAGAACGTTATGAAGCTATTGCT
AGTCGTGCTTTGGCAGATTTAGACACCTGTTTAGCGTCGCTTTAA

Upstream 100 bases:

>100_bases
GCCCTAAGTCAGGTGACTATCTTATTGAGAAAAAAGTTCGTGGTGGCAAACAAGTAATGTGTAGCAATGAGACCTGTGAT
TACAAGGAAGAAAAAATTAA

Downstream 100 bases:

>100_bases
CACTAAAAAAGAGTTCCTTTTCAGTTGAGTTGCTGAAGAGGACTTTTTTATTTGCTGATTTGAGCGCTAGCTATGCAAAA
GAATGCACAAAAAATACATG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRITKEATHSLFCFFPLSVIISQVTTLERFVGLSSKRCHSRRGISDLSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKL
YEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDREGYAKSVTAELE
NHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGFYFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYL
NCPMTKEEFMAFHEALTTAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAV
VQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLLFAGQMTG
VEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIA
SRALADLDTCLASL

Sequences:

>Translated_494_residues
MRITKEATHSLFCFFPLSVIISQVTTLERFVGLSSKRCHSRRGISDLSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKL
YEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDREGYAKSVTAELE
NHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGFYFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYL
NCPMTKEEFMAFHEALTTAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAV
VQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLLFAGQMTG
VEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIA
SRALADLDTCLASL
>Mature_494_residues
MRITKEATHSLFCFFPLSVIISQVTTLERFVGLSSKRCHSRRGISDLSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKL
YEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDREGYAKSVTAELE
NHPLIEVIRDEITEIPNDAITVIATGPLTSDALAEKIHAVNGGDGFYFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYL
NCPMTKEEFMAFHEALTTAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAV
VQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLLFAGQMTG
VEGYVESAASGLVAGINAARLFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIA
SRALADLDTCLASL

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367273, Length=91, Percent_Identity=40.6593406593407, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 54669; Mature: 54669

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITKEATHSLFCFFPLSVIISQVTTLERFVGLSSKRCHSRRGISDLSQSTATYINVIGA
CCCCHHHHHHEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
GLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKE
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
EMRRLDSIIMRNGEANRVPAGGAMAVDREGYAKSVTAELENHPLIEVIRDEITEIPNDAI
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCE
TVIATGPLTSDALAEKIHAVNGGDGFYFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYL
EEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
NCPMTKEEFMAFHEALTTAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGL
ECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCCCEECCCCCCCCCC
EYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
EFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLLFAGQMTGVEGYVESAASGLVAGINAAR
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHH
LFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIA
HHHHCCCEEECCHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRALADLDTCLASL
HHHHHHHHHHHHCC
>Mature Secondary Structure
MRITKEATHSLFCFFPLSVIISQVTTLERFVGLSSKRCHSRRGISDLSQSTATYINVIGA
CCCCHHHHHHEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHC
GLAGSEAAYQIAKRGIPVKLYEMRGVKATPQHKTTNFAELVCSNSFRGDSLTNAVGLLKE
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHH
EMRRLDSIIMRNGEANRVPAGGAMAVDREGYAKSVTAELENHPLIEVIRDEITEIPNDAI
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCE
TVIATGPLTSDALAEKIHAVNGGDGFYFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYL
EEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
NCPMTKEEFMAFHEALTTAEEAPLNSFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGL
ECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCCCEECCCCCCCCCC
EYPDDYTGPRDGEFKTPYAVVQLRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
EFVRYGVMHRNSYMDSPNLLTETFQSRSNPNLLFAGQMTGVEGYVESAASGLVAGINAAR
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHH
LFKREEALIFPQTTAIGSLPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIA
HHHHCCCEEECCHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
SRALADLDTCLASL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]