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The map label for this gene is pycB [H]

Identifier: 161485745

GI number: 161485745

Start: 876302

End: 877708

Strand: Direct

Name: pycB [H]

Synonym: SpyM3_0822

Alternate gene names: 161485745

Gene position: 876302-877708 (Clockwise)

Preceding gene: 21910357

Following gene: 21910359

Centisome position: 46.11

GC content: 42.08

Gene sequence:

>1407_bases
TTGATTCAGTTTGGAGGTTTTAGCATGCAGCAGCAAGTCGCAATAACAGAAACAGTTTTACGGGACGGTCATCAAAGTTT
GATGGCAACGCGTTTATCTATTGAGGATATGCTTCCTGTCCTTACCATCTTAGACAAGATTGGTTATTATTCCCTAGAGT
GTTGGGGAGGAGCAACCTTTGATGCCTGTATTCGTTTTTTGAATGAAGACCCTTGGGAAAGGCTGCGCACGTTAAAAAAA
GGCTTACCTAATACCCGTTTACAAATGTTATTAAGAGGCCAAAACCTTTTAGGCTACCGTCATTATGCAGATGACATCGT
TGACAAATTCATTAGTTTATCAGCTCAAAATGGGATTGATGTTTTCCGCATTTTTGATGCCCTAAATGACCCACGAAATA
TCCAGCAAGCCTTAAGAGCTGTGAAAAAGACGGGTAAAGAGGCTCAACTGTGTATTGCCTATACAACTAGCCCAGTGCAC
ACGCTTAACTATTACCTTTCTTTAGTGAAAGAATTGGTTGAGATGGGAGCTGATTCTATCTGCATCAAAGATATGGCTGG
TATTCTAACTCCCAAAGCAGCTAAAGAGTTGGTATCTGGTATCAAAGCCATGACTAACTTACCTCTTATTGTGCATACCC
ATGCAACTAGTGGCATTTCTCAGATGACCTATCTAGCAGCAGTAGAAGCTGGTGCTGATAGGATTGATACCGCTTTGTCA
CCTTTTAGCGAAGGCACTTCACAACCTGCTACAGAATCCATGTATTTAGCCTTAAAAGAAGCAGGTTATGATATCACATT
AGATGAAACGCTACTGGAACAGGCTGCTAATCACCTTCGACAAGCCCGTCAAAAGTACTTAGCAGATGGCATTCTGGATC
CCAGTCTTCTCTTTCCAGACCCAAGGACACTACAATACCAAGTTCCTGGAGGCATGTTATCTAATATGCTATCTCAGCTG
AAACAAGCCAATGCCGAATCAAAATTAGAAGAAGTTTTAGCAGAAGTTCCTCGGGTAAGAAAAGACTTGGGTTATCCTCC
CTTAGTAACACCGCTTAGCCAAATGGTCGGGACTCAAGCTGCTATGAATGTGATTTTAGGAAAACCTTACCAGATGGTGT
CTAAGGAAATTAAACAATACTTGGCAGGCGACTATGGCAAAACACCAGCACCAGTCAACGAAGACTTAAAACGAAGTCAA
ATTGGCTCAGCTCCTGTCACCACAAATCGTCCTGCAGATCAACTAAGTCCTGAATTCGAGGTGTTAAAAGCAGAAGTAGC
AGATTTAGCCCAAACGGACGAAGATGTGCTGACTTATGCCCTCTTCCCAAGCGTAGCAAAACCGTTTTTAACAACCAAAT
ACCAAACTGATGACGTCATTAAAGTGACTGCCTTTATCAAAGCATAA

Upstream 100 bases:

>100_bases
TGCTGATTTGAGCGCTAGCTATGCAAAAGAATGCACAAAAAATACATGAATAGACTATGGTCACTAGAAGCAATATTATA
TAATGAATAGTACTCATTAG

Downstream 100 bases:

>100_bases
TAGGCTATCTTAATCAGATAGAAACAAAAATATGGAGATCAAATGACCTATTCCTTTATTGATATCTTACAAGTTACCTT
AGTATCCTTGTTCTTAGTGT

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKK
GLPNTRLQMLLRGQNLLGYRHYADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISQMTYLAAVEAGADRIDTALS
PFSEGTSQPATESMYLALKEAGYDITLDETLLEQAANHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQL
KQANAESKLEEVLAEVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPVNEDLKRSQ
IGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFLTTKYQTDDVIKVTAFIKA

Sequences:

>Translated_468_residues
MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKK
GLPNTRLQMLLRGQNLLGYRHYADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISQMTYLAAVEAGADRIDTALS
PFSEGTSQPATESMYLALKEAGYDITLDETLLEQAANHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQL
KQANAESKLEEVLAEVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPVNEDLKRSQ
IGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFLTTKYQTDDVIKVTAFIKA
>Mature_468_residues
MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKK
GLPNTRLQMLLRGQNLLGYRHYADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISQMTYLAAVEAGADRIDTALS
PFSEGTSQPATESMYLALKEAGYDITLDETLLEQAANHLRQARQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQL
KQANAESKLEEVLAEVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPVNEDLKRSQ
IGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFLTTKYQTDDVIKVTAFIKA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=480, Percent_Identity=32.2916666666667, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI106049295, Length=480, Percent_Identity=32.2916666666667, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI106049292, Length=480, Percent_Identity=32.2916666666667, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17562816, Length=470, Percent_Identity=33.8297872340426, Blast_Score=251, Evalue=6e-67,
Organism=Saccharomyces cerevisiae, GI6319695, Length=467, Percent_Identity=34.0471092077088, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6321376, Length=467, Percent_Identity=34.475374732334, Blast_Score=250, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24652212, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652210, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652214, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19921944, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652216, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI281363050, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652224, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652222, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652220, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24652218, Length=480, Percent_Identity=33.75, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 51672; Mature: 51672

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSLECWGGATF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCH
DACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHYADDIVDKFISLSAQNGID
HHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHH
VFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVHTLNYYLSLVKELVEMGADSI
HHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
CIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISQMTYLAAVEAGADRIDTALS
HHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHC
PFSEGTSQPATESMYLALKEAGYDITLDETLLEQAANHLRQARQKYLADGILDPSLLFPD
CHHCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLAEVPRVRKDLGYPPLVTPLSQMVGTQA
CCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AMNVILGKPYQMVSKEIKQYLAGDYGKTPAPVNEDLKRSQIGSAPVTTNRPADQLSPEFE
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCHHH
VLKAEVADLAQTDEDVLTYALFPSVAKPFLTTKYQTDDVIKVTAFIKA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTILDKIGYYSLECWGGATF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCH
DACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHYADDIVDKFISLSAQNGID
HHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHH
VFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVHTLNYYLSLVKELVEMGADSI
HHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
CIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSGISQMTYLAAVEAGADRIDTALS
HHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHC
PFSEGTSQPATESMYLALKEAGYDITLDETLLEQAANHLRQARQKYLADGILDPSLLFPD
CHHCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLAEVPRVRKDLGYPPLVTPLSQMVGTQA
CCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AMNVILGKPYQMVSKEIKQYLAGDYGKTPAPVNEDLKRSQIGSAPVTTNRPADQLSPEFE
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCHHH
VLKAEVADLAQTDEDVLTYALFPSVAKPFLTTKYQTDDVIKVTAFIKA
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]