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The map label for this gene is folC.1 [H]

Identifier: 21910294

GI number: 21910294

Start: 812527

End: 813804

Strand: Direct

Name: folC.1 [H]

Synonym: SpyM3_0758

Alternate gene names: 21910294

Gene position: 812527-813804 (Clockwise)

Preceding gene: 21910293

Following gene: 21910295

Centisome position: 42.75

GC content: 40.22

Gene sequence:

>1278_bases
ATGACTTATGAAGAGACTCTAGAATGGATTCATGATCACTTAGTTTTCGGCATCAAGCCAGGCTTAAAACGTATGCTTTG
GGTTCTTGGACAGTTAGGAAATCCCCAAAAAAATGTCAAGGGAGTGCATATCGTTGGAACTAACGGAAAAGGTTCCACGG
TCAATCATTTACAACACATTTTTACGACAGCTGGTTATGAAGTGGGGACCTTTACCTCTCCTTATATTATGGATTTCAAA
GAACGTATTAGCATAAATGGTCGAATGATTTCAGAAAAAGATTTGGTTATTGCTGCTAATCGTATTCGTCCTTTGACAGA
GCGTCTAGTTCAAGAGACTGATTTTGGAGAGGTAACTGAATTTGAAGTGATTACTTTGATCATGTTCCTCTATTTTGGAG
ACATGCATCCTGTAGATATTGCTATTATTGAAGCAGGCCTTGGCGGTCTTTATGATTCGACCAATGTTTTTCAAGCAATG
GTTGTGGTCTGTCCCTCAATTGGTTTAGATCACCAAGCTATTTTGGGCGAGACTTACGCTGATATTGCCGCCCAAAAAGC
TGGTGTTTTAGAAGGGGGCGAGACCCTTGTTTTTGCCGTTGAAAACCCGTCAGCTCGAGAGGTTTTTTTAACAAAAGCTG
AGCAGGTTGGTGCTTCAATCTGGGAATGGCAAGAGCAGTTTCAAATGGCTGAGAACGCATCTGGCTATCGTTTTACTAGT
CCTTTAGGAGTTATTTCAGATATTCACATCGCTATGCCAGGACACCACCAAGTGTCTAATGCAGCTTTAGCTATTATGAC
TTGCTTAACCTTGAAAGACCGCTATCCTAGGCTAACTTCAGATCATATAAGGGAAGGTCTAGCAAATAGTTTGTGGCTAG
GCCGTACCGAATTATTAGCACCAAATCTAATGATTGATGGGGCTCATAACAATGAAAGCGTTGCTGCTTTGGTAGCCGTC
TTAAAAAATAACTACAATGATAAAAAGCTTCATATTTTGTTTGGTGCGATCGACACCAAGCCTATTGCAGATATGTTGGT
TGCTCTTGAGCAAATAGGTGACTTGCAGGTTACTAGCTTTCATTACCCTAACGCCTATCCATTGGAAAAATACCCAGAAC
GTTTTGGTAGGGTTGCTGATTTCAAAGATTTCTTGGCCCTGCGTAAGCATGCTAAAGCAGATGACTTTTTCGTGATTACA
GGGTCACTATATTTTATTTCAGAAATTAGACGGTATTGGAAAAAACATATTGAAAAAAGCGTTCTTTTAACCCATTAA

Upstream 100 bases:

>100_bases
GTGTTTGGTGAGGATAATAAAAAAAAGGGTACTACTCTAGGTTATCTGCTGTCACTTTTTTTTTATATATGGTATACTAA
AGATAGATGTGAGGTGTGAA

Downstream 100 bases:

>100_bases
AAAAGAGAAAATGAAAATGAAAAGGAAAAAACTAATGAAAAGGGAAAGACTAATGTCTATTAATAAGGAAAAAGCAGAAG
CAGCCATTTATCAGTTTTTA

Product: dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFK
ERISINGRMISEKDLVIAANRIRPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWEWQEQFQMAENASGYRFTS
PLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAV
LKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVIT
GSLYFISEIRRYWKKHIEKSVLLTH

Sequences:

>Translated_425_residues
MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFK
ERISINGRMISEKDLVIAANRIRPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWEWQEQFQMAENASGYRFTS
PLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAV
LKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVIT
GSLYFISEIRRYWKKHIEKSVLLTH
>Mature_424_residues
TYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKE
RISINGRMISEKDLVIAANRIRPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMV
VVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWEWQEQFQMAENASGYRFTSP
LGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVL
KNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSFHYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVITG
SLYFISEIRRYWKKHIEKSVLLTH

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=318, Percent_Identity=29.559748427673, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI66932990, Length=318, Percent_Identity=29.559748427673, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788654, Length=399, Percent_Identity=25.31328320802, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=344, Percent_Identity=25.5813953488372, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71984923, Length=344, Percent_Identity=25.5813953488372, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17553148, Length=344, Percent_Identity=25.5813953488372, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323760, Length=447, Percent_Identity=26.8456375838926, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324815, Length=302, Percent_Identity=28.476821192053, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24641571, Length=335, Percent_Identity=26.5671641791045, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581568, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47604; Mature: 47473

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
FTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRPLTERLVQETDFGEVTE
HHHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCEEEEEHHCCHHHHHHHHCCCCCCCHH
FEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVVCPSIGLDHQAILGETYA
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
DIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWEWQEQFQMAENASGYRFTS
HHHHHHCCCCCCCCEEEEEECCCCCCHHHEECHHHHCCHHHHHHHHHHHHHCCCCCEEEC
PLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGLANSLWLGRTELLA
CCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCC
PNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSF
CCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEE
HYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVITGSLYFISEIRRYWKKHIEKS
ECCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
VLLTH
HCCCC
>Mature Secondary Structure 
TYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHIVGTNGKGSTVNHLQHI
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
FTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRIRPLTERLVQETDFGEVTE
HHHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCEEEEEHHCCHHHHHHHHCCCCCCCHH
FEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAMVVVCPSIGLDHQAILGETYA
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
DIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGASIWEWQEQFQMAENASGYRFTS
HHHHHHCCCCCCCCEEEEEECCCCCCHHHEECHHHHCCHHHHHHHHHHHHHCCCCCEEEC
PLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYPRLTSDHIREGLANSLWLGRTELLA
CCCCHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCC
PNLMIDGAHNNESVAALVAVLKNNYNDKKLHILFGAIDTKPIADMLVALEQIGDLQVTSF
CCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCEEEEEE
HYPNAYPLEKYPERFGRVADFKDFLALRKHAKADDFFVITGSLYFISEIRRYWKKHIEKS
ECCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHH
VLLTH
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]