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The map label for this gene is acoC [H]

Identifier: 21910199

GI number: 21910199

Start: 725443

End: 726852

Strand: Direct

Name: acoC [H]

Synonym: SpyM3_0663

Alternate gene names: 21910199

Gene position: 725443-726852 (Clockwise)

Preceding gene: 21910198

Following gene: 21910200

Centisome position: 38.17

GC content: 42.77

Gene sequence:

>1410_bases
ATGGCATTTGAAATTATTATGCCAAAGCTGGGCGTTGACATGCAAGAAGGCGAGATCATCGAGTGGAAAAAACAAGAAGG
TGATACCGTCAATGAAGGTGATATCCTTCTTGAAATCATGTCAGACAAAACCAATATGGAACTTGAGGCAGAAGACTCAG
GTGTGCTATTGAAAATTGTCCGTCCAGCTGGTGATACTGTACCTGTAACTGAAGTTATCGGTTACATTGGTGCAGAGGGT
GAATCAGTAGATACTATTGCTTCAAGTGAAAAAACAACAGAAATTCCAGTTCCTGCTTCAGCAGACGCTGGACCAGCAGT
TGCTCCAAAAGAAAATGTGGCAAGTCCAGCTCCTCAAGTGGCAGCGACAGCTATTCCGCAAGGAAATGGCGGAAAAGTTC
GTGCTACTCCAGCTGCACGTAAGGCAGCAGCAGAAATGGGCATTGACCTTGGCCAAGTTCCAGGAACAGGACCAAAAGGA
CGTGTCCATAAAGAAGACGTTGAAAACTTTAAAGGTGCTCAACCGAAAGCTTCACCACTTGCTCGTAAAATTGCAGCAGA
CAAAGGTATTGATTTGGCAACCGTATCAGGTACTGGCTTTAACGGTAAGGTCATGAAAGAAGATATCATGGCTATTCTTG
AAGCGGCTAAACCAGCTGAAGCAAAAGCCCCAGCAGCTAAAGAAGAAAAAGTTGTTGATCTTCCAGAAGGTGTGGAACAC
AAGCCAATGTCAGCTATGCGTAAGGCAATCTCTAAAGGTATGACAAACTCTTACCTTACTGCTCCTACCTTTACGCTTAA
CTACGATATTGACATGACAGAGATGATTGCCCTTCGTAAGAAGTTAATTGACCCAATCATGGCTAAAACAGGCCTTAAAG
TCAGCTTCACAGACTTGATTGGTATGGCAGTGGTTAAAACCTTGATGAAACCTGAGCATGAGTACATGAATGCTTCCCTC
ATCAATGATGCTAATGATATTGAATTACACCGCTTTGTGAACCTTGGTATTGCCGTAGGTCTTGATGATGGTCTTATCGT
TCCTGTTATCCATGGGGCTGACAAGATGAGCTTGTCAGATTTTGTGCTTGCTTCAAAAGATGTGATTAAAAAAGCGCAAA
CTGGTAAGTTGAAAGCAGCTGAAATGTCTGGATCAACCTTCTCTATCACAAACTTGGGAATGTTTGGTACGAAGACATTT
AACCCAATTATTAACCAACCAAACTCAGCAATTCTTGGTGTTGGAGCAACTATCCCAACTCCAACGGTTGTGGATGGTGA
GATTGTTGCCCGTCCAATTATGGCAATGTGCCTAACCATCGACCACCGTTTAGTTGATGGTATGAACGGTGCAAAATTCA
TGGTTGATCTCAAGAAATTGATGGAAAATCCATTTGAATTGTTAATCTGA

Upstream 100 bases:

>100_bases
GAGTTGTCTTATCCCGTGTCGCGGCACCTCACCTTTCGTATGTTTTCGTAGCAAGGTGGGGATGTTGATGGCGCTTATCA
AATCGTGAAAGGATGTAGAA

Downstream 100 bases:

>100_bases
TCAATCAGTTTTAAACAAGAAAAGCATTCAATAATAAAAGGGATTTTGCTAAGTTTAGACTTGGTGGGTGCCCTTTTCTC
AAAAATATAGAAAGGATGTA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEG
ESVDTIASSEKTTEIPVPASADAGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKPAEAKAPAAKEEKVVDLPEGVEH
KPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMIALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASL
INDANDIELHRFVNLGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNLGMFGTKTF
NPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNGAKFMVDLKKLMENPFELLI

Sequences:

>Translated_469_residues
MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEG
ESVDTIASSEKTTEIPVPASADAGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
RVHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKPAEAKAPAAKEEKVVDLPEGVEH
KPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMIALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASL
INDANDIELHRFVNLGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNLGMFGTKTF
NPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNGAKFMVDLKKLMENPFELLI
>Mature_468_residues
AFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGE
SVDTIASSEKTTEIPVPASADAGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKGR
VHKEDVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKPAEAKAPAAKEEKVVDLPEGVEHK
PMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMIALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLI
NDANDIELHRFVNLGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNLGMFGTKTFN
PIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNGAKFMVDLKKLMENPFELLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=475, Percent_Identity=30.5263157894737, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI203098753, Length=479, Percent_Identity=29.0187891440501, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI203098816, Length=479, Percent_Identity=29.0187891440501, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI110671329, Length=468, Percent_Identity=26.9230769230769, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=31.0344827586207, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI260898739, Length=141, Percent_Identity=34.0425531914894, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI1786946, Length=472, Percent_Identity=26.9067796610169, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1786305, Length=293, Percent_Identity=33.1058020477816, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17560088, Length=478, Percent_Identity=29.9163179916318, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=459, Percent_Identity=28.5403050108932, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=460, Percent_Identity=25.2173913043478, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=324, Percent_Identity=28.3950617283951, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320352, Length=466, Percent_Identity=28.5407725321888, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=176, Percent_Identity=34.0909090909091, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321632, Length=337, Percent_Identity=25.2225519287834, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24645909, Length=246, Percent_Identity=31.7073170731707, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI18859875, Length=465, Percent_Identity=26.0215053763441, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24582497, Length=304, Percent_Identity=28.6184210526316, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129315, Length=304, Percent_Identity=28.6184210526316, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 49738; Mature: 49607

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIV
CCEEEECHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEE
RPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASADAGPAVAPKENVASPAPQV
ECCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
AATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKGRVHKEDVENFKGAQPKASPL
HHEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHH
ARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKPAEAKAPAAKEEKVVDLPEGVEH
HHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCC
KPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMIALRKKLIDPIMAKTGLKVSFTDLI
CCHHHHHHHHHCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
GMAVVKTLMKPEHEYMNASLINDANDIELHRFVNLGIAVGLDDGLIVPVIHGADKMSLSD
HHHHHHHHHCCCHHHCCHHHCCCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCHHH
FVLASKDVIKKAQTGKLKAAEMSGSTFSITNLGMFGTKTFNPIINQPNSAILGVGATIPT
HHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHCCCCCEEEEECCCCCC
PTVVDGEIVARPIMAMCLTIDHRLVDGMNGAKFMVDLKKLMENPFELLI
CCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
AFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKTNMELEAEDSGVLLKIV
CEEEECHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEE
RPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASADAGPAVAPKENVASPAPQV
ECCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
AATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKGRVHKEDVENFKGAQPKASPL
HHEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHH
ARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKPAEAKAPAAKEEKVVDLPEGVEH
HHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCC
KPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMIALRKKLIDPIMAKTGLKVSFTDLI
CCHHHHHHHHHCCCCCCEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
GMAVVKTLMKPEHEYMNASLINDANDIELHRFVNLGIAVGLDDGLIVPVIHGADKMSLSD
HHHHHHHHHCCCHHHCCHHHCCCCCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCHHH
FVLASKDVIKKAQTGKLKAAEMSGSTFSITNLGMFGTKTFNPIINQPNSAILGVGATIPT
HHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCCCHHHCCCCCEEEEECCCCCC
PTVVDGEIVARPIMAMCLTIDHRLVDGMNGAKFMVDLKKLMENPFELLI
CCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA