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The map label for this gene is dapE [H]

Identifier: 30410814

GI number: 30410814

Start: 99143

End: 100297

Strand: Direct

Name: dapE [H]

Synonym: BUsg087

Alternate gene names: 30410814

Gene position: 99143-100297 (Clockwise)

Preceding gene: 21672378

Following gene: 21672381

Centisome position: 15.46

GC content: 26.84

Gene sequence:

>1155_bases
ATGAAAGAAGATATACATGAGGAAAAAATGGTTTGTTCAATTACTAAATTAGCACAAAAGTTAATTTCAATTCCATCTAT
TAGTCCAAAAGACTTAGGTTGTCAAGATATTATGATTGATTTTTTAGATAATCTTGGATTTGAAATAAAAAAAATTAATA
TAAATGATACGAAAAATTTTTGGGCTACTAGAGGATTTGGTAAAACTTTAACATTTGCTGGTCATACAGATGTCGTACCA
CCAGGTGATTATAAATATTGGAATGATGATCCTTTCAATCCAGTAATAAAAAATGGTTTATTATTTGGTCGAGGTTCATC
AGATATGAAAGGTGCCTTAGCATCTATGTTAATTGCATCTGAAAGATTTATAAAAAAAAATCCTAACTATGAAGGGCGTT
TGTCTTTTTTAATTACTTCAGATGAAGAGTCATCCGCGATTGATGGTACTAAAAAAGTTATAGATTATTTAATTTCTCGA
AAAGATACGATTGATTATTGTGTTATTGGAGAACCTTGTAGTAGTTCTAAAATTGGTGATGTTATAAAAAATGGACGTAG
AGGTTCTATAACAGCTAATTTAACAATTCATGGAATTCAAGGTCATATTGCATACCCTCATTTAGCAGATAATCCCATAC
ATAAGGGATTACCTATTATTTTAAAAATATTATCTATTAAACTAGATGATGGTAATGCATTTTTTGCTCCAACTAGTGTC
AATATTTCTAATATTCATGCAGGAGATGGAATTAATAATATTATTCCAGGTTCTTTGTTTGTTCAGTTTAATTTTCGTTT
TAGTACAGAAACTTCTGAAAAAGAGATCAAATCAAAATTTACACAAATATTAGATAATAACAATATTAATTATTCTCTAA
ATTGGTTTTTTTCAGGAAATCCTTTTATTACAAAAAAAGGTTTGTTAATAGATAATATAACTAAATCTATTATAAATTTA
AATAAAGTTAAACCTGTTTTATCAACTGATGGAGGAACTTCAGATGGTCGTTTTATTGCTTCAATGAATGCCGAAATAGT
AGAATTAGGTTTAATGAATAATACAATTCATAAATCAAATGAATATGTAAGAATATCTGATTTAGAAAAATTAGCTATTA
TTTATGAAGATATTATGTACAGGTTATTGACTTAG

Upstream 100 bases:

>100_bases
ATATAGTAAATAAATCTAAAATTTTATTGAAACATTAATTTTAAACTTTTTTATGATATTAATGGGGGCTTTTTTCTTAG
CCCCAATTATTTGAATAAAT

Downstream 100 bases:

>100_bases
TTTTATAGTTTTTTTATTTATTAAAATAATGATGCAGAATATCATTTAAGATAATCTGCACCCATTTTTTTAAATTTTCA
ATAATAGATTTTTTTTATAT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKEDIHEEKMVCSITKLAQKLISIPSISPKDLGCQDIMIDFLDNLGFEIKKININDTKNFWATRGFGKTLTFAGHTDVVP
PGDYKYWNDDPFNPVIKNGLLFGRGSSDMKGALASMLIASERFIKKNPNYEGRLSFLITSDEESSAIDGTKKVIDYLISR
KDTIDYCVIGEPCSSSKIGDVIKNGRRGSITANLTIHGIQGHIAYPHLADNPIHKGLPIILKILSIKLDDGNAFFAPTSV
NISNIHAGDGINNIIPGSLFVQFNFRFSTETSEKEIKSKFTQILDNNNINYSLNWFFSGNPFITKKGLLIDNITKSIINL
NKVKPVLSTDGGTSDGRFIASMNAEIVELGLMNNTIHKSNEYVRISDLEKLAIIYEDIMYRLLT

Sequences:

>Translated_384_residues
MKEDIHEEKMVCSITKLAQKLISIPSISPKDLGCQDIMIDFLDNLGFEIKKININDTKNFWATRGFGKTLTFAGHTDVVP
PGDYKYWNDDPFNPVIKNGLLFGRGSSDMKGALASMLIASERFIKKNPNYEGRLSFLITSDEESSAIDGTKKVIDYLISR
KDTIDYCVIGEPCSSSKIGDVIKNGRRGSITANLTIHGIQGHIAYPHLADNPIHKGLPIILKILSIKLDDGNAFFAPTSV
NISNIHAGDGINNIIPGSLFVQFNFRFSTETSEKEIKSKFTQILDNNNINYSLNWFFSGNPFITKKGLLIDNITKSIINL
NKVKPVLSTDGGTSDGRFIASMNAEIVELGLMNNTIHKSNEYVRISDLEKLAIIYEDIMYRLLT
>Mature_384_residues
MKEDIHEEKMVCSITKLAQKLISIPSISPKDLGCQDIMIDFLDNLGFEIKKININDTKNFWATRGFGKTLTFAGHTDVVP
PGDYKYWNDDPFNPVIKNGLLFGRGSSDMKGALASMLIASERFIKKNPNYEGRLSFLITSDEESSAIDGTKKVIDYLISR
KDTIDYCVIGEPCSSSKIGDVIKNGRRGSITANLTIHGIQGHIAYPHLADNPIHKGLPIILKILSIKLDDGNAFFAPTSV
NISNIHAGDGINNIIPGSLFVQFNFRFSTETSEKEIKSKFTQILDNNNINYSLNWFFSGNPFITKKGLLIDNITKSIINL
NKVKPVLSTDGGTSDGRFIASMNAEIVELGLMNNTIHKSNEYVRISDLEKLAIIYEDIMYRLLT

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=54.1333333333333, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1790395, Length=260, Percent_Identity=30, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789236, Length=236, Percent_Identity=26.6949152542373, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 42738; Mature: 42738

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDIHEEKMVCSITKLAQKLISIPSISPKDLGCQDIMIDFLDNLGFEIKKININDTKNF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
WATRGFGKTLTFAGHTDVVPPGDYKYWNDDPFNPVIKNGLLFGRGSSDMKGALASMLIAS
EEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHH
ERFIKKNPNYEGRLSFLITSDEESSAIDGTKKVIDYLISRKDTIDYCVIGEPCSSSKIGD
HHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHH
VIKNGRRGSITANLTIHGIQGHIAYPHLADNPIHKGLPIILKILSIKLDDGNAFFAPTSV
HHHCCCCCEEEEEEEEEEEECEEECCCCCCCCCCCCHHHHEEEEEEEECCCCEEEEECCC
NISNIHAGDGINNIIPGSLFVQFNFRFSTETSEKEIKSKFTQILDNNNINYSLNWFFSGN
CEEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
PFITKKGLLIDNITKSIINLNKVKPVLSTDGGTSDGRFIASMNAEIVELGLMNNTIHKSN
EEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCEEEEEECCCCCEECCC
EYVRISDLEKLAIIYEDIMYRLLT
CEEEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEDIHEEKMVCSITKLAQKLISIPSISPKDLGCQDIMIDFLDNLGFEIKKININDTKNF
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCC
WATRGFGKTLTFAGHTDVVPPGDYKYWNDDPFNPVIKNGLLFGRGSSDMKGALASMLIAS
EEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEEECCCCHHHHHHHHHHHHH
ERFIKKNPNYEGRLSFLITSDEESSAIDGTKKVIDYLISRKDTIDYCVIGEPCSSSKIGD
HHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHH
VIKNGRRGSITANLTIHGIQGHIAYPHLADNPIHKGLPIILKILSIKLDDGNAFFAPTSV
HHHCCCCCEEEEEEEEEEEECEEECCCCCCCCCCCCHHHHEEEEEEEECCCCEEEEECCC
NISNIHAGDGINNIIPGSLFVQFNFRFSTETSEKEIKSKFTQILDNNNINYSLNWFFSGN
CEEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCC
PFITKKGLLIDNITKSIINLNKVKPVLSTDGGTSDGRFIASMNAEIVELGLMNNTIHKSN
EEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCEEEEEECCCCCEECCC
EYVRISDLEKLAIIYEDIMYRLLT
CEEEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]