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The map label for this gene is gltX [H]

Identifier: 30410812

GI number: 30410812

Start: 75095

End: 76552

Strand: Direct

Name: gltX [H]

Synonym: BUsg065

Alternate gene names: 30410812

Gene position: 75095-76552 (Clockwise)

Preceding gene: 21672351

Following gene: 21672359

Centisome position: 11.71

GC content: 24.97

Gene sequence:

>1458_bases
ATGAATCAAATAAAATTTTTAGTTAAGGAACTTATGAAAGTAAAAACTCGTTTTGCACCTAGTCCTACTGGAAATTTACA
TATTGGTAGTATTCGTACGGCTTTGTATTCTTGGTTATTTGCACGTCACTATCATGGAAAATTCATTCTTCGTATCGAAG
ATACAGATTTTGAACGTTTTGATTCAAAATCCGTTGAATCTATTTTATATGGATTAAAATGGTTAGGATTAAATTGGGAT
GAAGGTCCTTATTTCCAGAGTAAAAGATTAGAAAGGTATAAAGAAGTTATTAAAATGATGTTAGAATCAGGTAATGCTTA
TAAATGTTTTTGTTCAGTAAAAGAATTAGAAGATGAGCGAATAAGACAAGTTTCTAAAGGAAAAAAACCACGATACAGTG
GAATTTGTAGAAATTTACATTATAGAAATTATATTGCTAATGAAAATTATGTAGTACGATTTAAAAATCCGATTTCAGGT
CAAGTAACATTCGAAGATAAAATTAGAGGTAAGATTACTTTTCAAAACGAAGAATTAGATGATCTTGTAATTCAACGTTC
TAACGGAATTCCTACATATAATTTTTGCGTGGTGATAGATGATTTAGATATGAATATAACTCATGTTATTCGTGGTGAAG
ATCATATTAATAATACTCCTCGTCAAATAAATATTTTAAAATCCTTAAATGCTAAAATACCTATTTATGCTCATGTTTCA
ATGATTTTTAATGAAGATGGACATAAATTTTCTAAAAGACAAGATGCTTTGAACATTTTAGAATATTGTAAAGATGGTTT
TTTACCAGAAGCTTTACTGAATTATGTAATACGTTTAGGTTGGTCTTGTGGTAATCAAGAGATTTTTAGTATTTCAGAAA
TGAAAGAATTATTTGATTTAAGAAAAATTAGTAAGTCTTCTAGTGCTGTAAGTATGAAAAAACTTTTTTGGTTAAATAAA
TATTACATTAATAATTTACCGTTAAGTTATATTTCGAATCTTTTAAAAGATCATATGAATCATGAAAAGATTAATTTAAA
AAATGGACCTGATTTAGAATCTGTTTTGACTTTATTAAAAAATCGTCATCATACTTTAAAAGAAATTGCTCAATCTTCTA
GATATTTTTATGAAGAATTTGCTAATTTTGATAAATCAGCAGCAGATGAATATTTAATTCCAGAAAATCATTTTATTTTA
AAAAATTTATATAAGAATATAAAAAAAATATCTATTTGGAGTAATGAGACATTATCTTCATTGATTAAAAATGATTGCAT
TAAACTTAAAATTACATTAAGAAAAATAAGCATGTTATTACGTGTTGTATTAACAGGAAATATATTTTCGCCGAGTATAA
GTTCTGTGATTTTTTTAATTGGAAAAGAGAAGACTTTATTAAGACTAAAAAAAGCAATTTTATTTATTGATAATAAAATT
AAAAATATAAATAATTAG

Upstream 100 bases:

>100_bases
TTGTTTGTTGTAAATTTAATCATTATGATTAAATAATTATCTATAATTTAATATTTATAAAATTAATCTATAGAATTTTA
AAAAATGTAATATGAAGTAT

Downstream 100 bases:

>100_bases
AAAAGAATAACTATTAATGAAAAAAATTAACAAATTGTTTTCACTTAAAGTCGTTTTAAAATTAATTAAAAGAAATTTGA
TAGATCTTTTTTAATAAGAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNQIKFLVKELMKVKTRFAPSPTGNLHIGSIRTALYSWLFARHYHGKFILRIEDTDFERFDSKSVESILYGLKWLGLNWD
EGPYFQSKRLERYKEVIKMMLESGNAYKCFCSVKELEDERIRQVSKGKKPRYSGICRNLHYRNYIANENYVVRFKNPISG
QVTFEDKIRGKITFQNEELDDLVIQRSNGIPTYNFCVVIDDLDMNITHVIRGEDHINNTPRQINILKSLNAKIPIYAHVS
MIFNEDGHKFSKRQDALNILEYCKDGFLPEALLNYVIRLGWSCGNQEIFSISEMKELFDLRKISKSSSAVSMKKLFWLNK
YYINNLPLSYISNLLKDHMNHEKINLKNGPDLESVLTLLKNRHHTLKEIAQSSRYFYEEFANFDKSAADEYLIPENHFIL
KNLYKNIKKISIWSNETLSSLIKNDCIKLKITLRKISMLLRVVLTGNIFSPSISSVIFLIGKEKTLLRLKKAILFIDNKI
KNINN

Sequences:

>Translated_485_residues
MNQIKFLVKELMKVKTRFAPSPTGNLHIGSIRTALYSWLFARHYHGKFILRIEDTDFERFDSKSVESILYGLKWLGLNWD
EGPYFQSKRLERYKEVIKMMLESGNAYKCFCSVKELEDERIRQVSKGKKPRYSGICRNLHYRNYIANENYVVRFKNPISG
QVTFEDKIRGKITFQNEELDDLVIQRSNGIPTYNFCVVIDDLDMNITHVIRGEDHINNTPRQINILKSLNAKIPIYAHVS
MIFNEDGHKFSKRQDALNILEYCKDGFLPEALLNYVIRLGWSCGNQEIFSISEMKELFDLRKISKSSSAVSMKKLFWLNK
YYINNLPLSYISNLLKDHMNHEKINLKNGPDLESVLTLLKNRHHTLKEIAQSSRYFYEEFANFDKSAADEYLIPENHFIL
KNLYKNIKKISIWSNETLSSLIKNDCIKLKITLRKISMLLRVVLTGNIFSPSISSVIFLIGKEKTLLRLKKAILFIDNKI
KNINN
>Mature_485_residues
MNQIKFLVKELMKVKTRFAPSPTGNLHIGSIRTALYSWLFARHYHGKFILRIEDTDFERFDSKSVESILYGLKWLGLNWD
EGPYFQSKRLERYKEVIKMMLESGNAYKCFCSVKELEDERIRQVSKGKKPRYSGICRNLHYRNYIANENYVVRFKNPISG
QVTFEDKIRGKITFQNEELDDLVIQRSNGIPTYNFCVVIDDLDMNITHVIRGEDHINNTPRQINILKSLNAKIPIYAHVS
MIFNEDGHKFSKRQDALNILEYCKDGFLPEALLNYVIRLGWSCGNQEIFSISEMKELFDLRKISKSSSAVSMKKLFWLNK
YYINNLPLSYISNLLKDHMNHEKINLKNGPDLESVLTLLKNRHHTLKEIAQSSRYFYEEFANFDKSAADEYLIPENHFIL
KNLYKNIKKISIWSNETLSSLIKNDCIKLKITLRKISMLLRVVLTGNIFSPSISSVIFLIGKEKTLLRLKKAILFIDNKI
KNINN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=331, Percent_Identity=35.3474320241692, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI1788743, Length=465, Percent_Identity=53.9784946236559, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=37.3540856031128, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786895, Length=211, Percent_Identity=27.9620853080569, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=478, Percent_Identity=32.6359832635983, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=31.6831683168317, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321192, Length=223, Percent_Identity=29.5964125560538, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324742, Length=217, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=31.1608961303462, Blast_Score=220, Evalue=2e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56758; Mature: 56758

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIKFLVKELMKVKTRFAPSPTGNLHIGSIRTALYSWLFARHYHGKFILRIEDTDFERF
CCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
DSKSVESILYGLKWLGLNWDEGPYFQSKRLERYKEVIKMMLESGNAYKCFCSVKELEDER
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
IRQVSKGKKPRYSGICRNLHYRNYIANENYVVRFKNPISGQVTFEDKIRGKITFQNEELD
HHHHHCCCCCCHHHHHHHCHHHHEECCCCEEEEECCCCCCEEEEECCCCEEEEECCCCHH
DLVIQRSNGIPTYNFCVVIDDLDMNITHVIRGEDHINNTPRQINILKSLNAKIPIYAHVS
HHHHCCCCCCCEEEEEEEEECCCCCEEEEECCCHHCCCCCHHHHHHHHCCCCCCEEEEEE
MIFNEDGHKFSKRQDALNILEYCKDGFLPEALLNYVIRLGWSCGNQEIFSISEMKELFDL
EEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHH
RKISKSSSAVSMKKLFWLNKYYINNLPLSYISNLLKDHMNHEKINLKNGPDLESVLTLLK
HHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
NRHHTLKEIAQSSRYFYEEFANFDKSAADEYLIPENHFILKNLYKNIKKISIWSNETLSS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCHHHHH
LIKNDCIKLKITLRKISMLLRVVLTGNIFSPSISSVIFLIGKEKTLLRLKKAILFIDNKI
HHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHHHH
KNINN
CCCCC
>Mature Secondary Structure
MNQIKFLVKELMKVKTRFAPSPTGNLHIGSIRTALYSWLFARHYHGKFILRIEDTDFERF
CCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
DSKSVESILYGLKWLGLNWDEGPYFQSKRLERYKEVIKMMLESGNAYKCFCSVKELEDER
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHH
IRQVSKGKKPRYSGICRNLHYRNYIANENYVVRFKNPISGQVTFEDKIRGKITFQNEELD
HHHHHCCCCCCHHHHHHHCHHHHEECCCCEEEEECCCCCCEEEEECCCCEEEEECCCCHH
DLVIQRSNGIPTYNFCVVIDDLDMNITHVIRGEDHINNTPRQINILKSLNAKIPIYAHVS
HHHHCCCCCCCEEEEEEEEECCCCCEEEEECCCHHCCCCCHHHHHHHHCCCCCCEEEEEE
MIFNEDGHKFSKRQDALNILEYCKDGFLPEALLNYVIRLGWSCGNQEIFSISEMKELFDL
EEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHH
RKISKSSSAVSMKKLFWLNKYYINNLPLSYISNLLKDHMNHEKINLKNGPDLESVLTLLK
HHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
NRHHTLKEIAQSSRYFYEEFANFDKSAADEYLIPENHFILKNLYKNIKKISIWSNETLSS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEECCCHHHHH
LIKNDCIKLKITLRKISMLLRVVLTGNIFSPSISSVIFLIGKEKTLLRLKKAILFIDNKI
HHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHEEEEECCCHHHHHHHHHHHHHHHHH
KNINN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]