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The map label for this gene is fliF

Identifier: 21672359

GI number: 21672359

Start: 77554

End: 79224

Strand: Direct

Name: fliF

Synonym: BUsg067

Alternate gene names: 21672359

Gene position: 77554-79224 (Clockwise)

Preceding gene: 30410812

Following gene: 21672360

Centisome position: 12.09

GC content: 24.0

Gene sequence:

>1671_bases
ATGAATTTCAGTACTATAGAAGAATCCGTTTCAGAAGAGAAAAAAAAATTTAATAATTTTTTGTCTTATTTTTTTAAAAA
TTCACGTGTTTTAATTATTTTATTTGTATTAGCTGTAATTACTACTGTTTCAATTTCTATGTGGCGTAAATCTCCTGATT
ATCAAGTTTTATATAATAATTTATCTAATGAAGATGGAGAAATGATCATTGATCAATTAAATCAAATGCAAATACCCTAT
AAACTTTCTGAAGATTCTGGTCAATTGTTAGTTCCAAAAGATAAAGTTTATGAACTTCGTTTGCATTTTTCTGAAAATAA
TTCTCCTCATAGAGATATAGGTTATGAAATTTTAGATAAAGAAAGATTTGGAGTTAGTCAATTTGGTGAGCAAATTAATT
ATCAACGTGCTTTAGAAGGGGAATTAGCTCGTACTATAGAAAAAATTAATGTTGTAAAAAATGCTAAAATACACATAGCT
TTTCCAAAAAATTCTTTATTTTTAGAAGATAAAAAAAAACCATCAGTTTCTGTTATTTTAAATTTAAAATCTAATCAAGG
ATTAGATCATAGTCAAGTTAATGCTATATTGCATTTAATTTCTAGTAGTATATGTGATTTATCTATAGAAAATATAACAA
TAATAGATCAGTTTGGAAAATTGTTAAATAATTCATCATTGGGTTTAAATCAAATTGATGATTTAAAACTCAGATATTCT
GAAGAAGTTGAATCTCGTTATAGAAATAGAATTAAAAACATTTTAGAACCTTTATTGGGGTTTAATAATGTTTATGCTCA
AGTTACTGCACAAATAAATTTTAATTCTCACGAAAAAACACAAGAAAAATATACACCTAATACAAATTATAAAAATCAAG
CAATACGATCTCGTCAAAGTACTGTTAACGACAAGATAAATAATAGAAAAGAAGAAAATAAACCAGACGAACTTTTTCCC
CAAACTAGTTTTTCTTCGAATAAGGATTTAAATAGTACAACATATAGTAATAAAAAAATTAAAAAATCTATTATACAAAA
CAATCAAGATAATAATATTTTACATTCTAATTCTGCTATTAGTCATGATGATACAATAAATTATGAGTTGAATCATAGTC
TTTCTCACACTAAAATGAATATAGGAGAAATAAAAAGATTGTCAGCAGCAGTAATAGTTAATTTCGTTAAAGATAAAAAT
GGAAAATCAGTTCCTATAAATGTAGAACAGATCAAAAAGATTAAAAATTTAGTACGCGAAGCAATAGGTTATTCCAAAGT
TAGAGGAGATAGCGTATATGTTGTTAATGAATCTTTTTTTCAAAAAAACAAAAATTCTCCTATTAAATTATTGAAAGATT
CTAATCAATCTAATTTTTATAGTACTTTTTTAACTTTCACACCTTGGTTCATTTCTCTTTTCTTTCTTTTTTTTCTTGTA
AAAAAATGTTTTTTTTCTTCTTCAAAAAACAATATTAATAATCAATCATATAAAAATAAAACTGAAGAAGATTTATTAGA
AAAAGATACAAAAGCAGAAAATATTTCTGAATTAAAATTTTCAAAAACTTCGAATACAGATAAATTAATTCATCAAATTT
GCAATATATCTAATCAAAATCCACGTATTATAGCGTCGATTATTCGTCAATGGATGAGTGATAAAAAATGA

Upstream 100 bases:

>100_bases
ATGTTTTTAATTTATCAGGAAATGTTTTTATTTCAAGTGAAATTTATTATTTGTGATTCAATTATATAGTTAGGATTAAC
TATTAGATTAGGAAGATGTT

Downstream 100 bases:

>100_bases
CTTTAAATGGTACTGAGAAAAGTGCAATTTTATTGATGTCAATAGGAGCTGATCAAGCATCAGAAGTATTAAAACATTTA
ACTCCTTTTGAAGTTCAAGA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MNFSTIEESVSEEKKKFNNFLSYFFKNSRVLIILFVLAVITTVSISMWRKSPDYQVLYNNLSNEDGEMIIDQLNQMQIPY
KLSEDSGQLLVPKDKVYELRLHFSENNSPHRDIGYEILDKERFGVSQFGEQINYQRALEGELARTIEKINVVKNAKIHIA
FPKNSLFLEDKKKPSVSVILNLKSNQGLDHSQVNAILHLISSSICDLSIENITIIDQFGKLLNNSSLGLNQIDDLKLRYS
EEVESRYRNRIKNILEPLLGFNNVYAQVTAQINFNSHEKTQEKYTPNTNYKNQAIRSRQSTVNDKINNRKEENKPDELFP
QTSFSSNKDLNSTTYSNKKIKKSIIQNNQDNNILHSNSAISHDDTINYELNHSLSHTKMNIGEIKRLSAAVIVNFVKDKN
GKSVPINVEQIKKIKNLVREAIGYSKVRGDSVYVVNESFFQKNKNSPIKLLKDSNQSNFYSTFLTFTPWFISLFFLFFLV
KKCFFSSSKNNINNQSYKNKTEEDLLEKDTKAENISELKFSKTSNTDKLIHQICNISNQNPRIIASIIRQWMSDKK

Sequences:

>Translated_556_residues
MNFSTIEESVSEEKKKFNNFLSYFFKNSRVLIILFVLAVITTVSISMWRKSPDYQVLYNNLSNEDGEMIIDQLNQMQIPY
KLSEDSGQLLVPKDKVYELRLHFSENNSPHRDIGYEILDKERFGVSQFGEQINYQRALEGELARTIEKINVVKNAKIHIA
FPKNSLFLEDKKKPSVSVILNLKSNQGLDHSQVNAILHLISSSICDLSIENITIIDQFGKLLNNSSLGLNQIDDLKLRYS
EEVESRYRNRIKNILEPLLGFNNVYAQVTAQINFNSHEKTQEKYTPNTNYKNQAIRSRQSTVNDKINNRKEENKPDELFP
QTSFSSNKDLNSTTYSNKKIKKSIIQNNQDNNILHSNSAISHDDTINYELNHSLSHTKMNIGEIKRLSAAVIVNFVKDKN
GKSVPINVEQIKKIKNLVREAIGYSKVRGDSVYVVNESFFQKNKNSPIKLLKDSNQSNFYSTFLTFTPWFISLFFLFFLV
KKCFFSSSKNNINNQSYKNKTEEDLLEKDTKAENISELKFSKTSNTDKLIHQICNISNQNPRIIASIIRQWMSDKK
>Mature_556_residues
MNFSTIEESVSEEKKKFNNFLSYFFKNSRVLIILFVLAVITTVSISMWRKSPDYQVLYNNLSNEDGEMIIDQLNQMQIPY
KLSEDSGQLLVPKDKVYELRLHFSENNSPHRDIGYEILDKERFGVSQFGEQINYQRALEGELARTIEKINVVKNAKIHIA
FPKNSLFLEDKKKPSVSVILNLKSNQGLDHSQVNAILHLISSSICDLSIENITIIDQFGKLLNNSSLGLNQIDDLKLRYS
EEVESRYRNRIKNILEPLLGFNNVYAQVTAQINFNSHEKTQEKYTPNTNYKNQAIRSRQSTVNDKINNRKEENKPDELFP
QTSFSSNKDLNSTTYSNKKIKKSIIQNNQDNNILHSNSAISHDDTINYELNHSLSHTKMNIGEIKRLSAAVIVNFVKDKN
GKSVPINVEQIKKIKNLVREAIGYSKVRGDSVYVVNESFFQKNKNSPIKLLKDSNQSNFYSTFLTFTPWFISLFFLFFLV
KKCFFSSSKNNINNQSYKNKTEEDLLEKDTKAENISELKFSKTSNTDKLIHQICNISNQNPRIIASIIRQWMSDKK

Specific function: The M ring may be actively involved in energy transduction

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family

Homologues:

Organism=Escherichia coli, GI1788248, Length=545, Percent_Identity=37.9816513761468, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLIF_BUCAP (Q8KA45)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660426.1
- ProteinModelPortal:   Q8KA45
- EnsemblBacteria:   EBBUCT00000000006
- GeneID:   1005883
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg067
- GeneTree:   EBGT00050000007726
- HOGENOM:   HBG661951
- OMA:   FMEATRY
- ProtClustDB:   PRK06007
- BioCyc:   BAPH198804:BUSG067-MONOMER
- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182
- PRINTS:   PR01009
- TIGRFAMs:   TIGR00206

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C

EC number: NA

Molecular weight: Translated: 64261; Mature: 64261

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbc4aa34)-; HASH(0x40450fa8)-;

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSTIEESVSEEKKKFNNFLSYFFKNSRVLIILFVLAVITTVSISMWRKSPDYQVLYNN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
LSNEDGEMIIDQLNQMQIPYKLSEDSGQLLVPKDKVYELRLHFSENNSPHRDIGYEILDK
CCCCCHHHHHHHHHHCCCCEEECCCCCCEECCCCCEEEEEEEECCCCCCCCCCCHHHHHH
ERFGVSQFGEQINYQRALEGELARTIEKINVVKNAKIHIAFPKNSLFLEDKKKPSVSVIL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCEEEEE
NLKSNQGLDHSQVNAILHLISSSICDLSIENITIIDQFGKLLNNSSLGLNQIDDLKLRYS
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHH
EEVESRYRNRIKNILEPLLGFNNVYAQVTAQINFNSHEKTQEKYTPNTNYKNQAIRSRQS
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHH
TVNDKINNRKEENKPDELFPQTSFSSNKDLNSTTYSNKKIKKSIIQNNQDNNILHSNSAI
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCC
SHDDTINYELNHSLSHTKMNIGEIKRLSAAVIVNFVKDKNGKSVPINVEQIKKIKNLVRE
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
AIGYSKVRGDSVYVVNESFFQKNKNSPIKLLKDSNQSNFYSTFLTFTPWFISLFFLFFLV
HHCHHHCCCCEEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
KKCFFSSSKNNINNQSYKNKTEEDLLEKDTKAENISELKFSKTSNTDKLIHQICNISNQN
HHHHHCCCCCCCCCCHHCCCCHHHHHHHCCCCCCHHHHEECCCCCHHHHHHHHHCCCCCC
PRIIASIIRQWMSDKK
CHHHHHHHHHHHCCCH
>Mature Secondary Structure
MNFSTIEESVSEEKKKFNNFLSYFFKNSRVLIILFVLAVITTVSISMWRKSPDYQVLYNN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHC
LSNEDGEMIIDQLNQMQIPYKLSEDSGQLLVPKDKVYELRLHFSENNSPHRDIGYEILDK
CCCCCHHHHHHHHHHCCCCEEECCCCCCEECCCCCEEEEEEEECCCCCCCCCCCHHHHHH
ERFGVSQFGEQINYQRALEGELARTIEKINVVKNAKIHIAFPKNSLFLEDKKKPSVSVIL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCEEEEE
NLKSNQGLDHSQVNAILHLISSSICDLSIENITIIDQFGKLLNNSSLGLNQIDDLKLRYS
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHH
EEVESRYRNRIKNILEPLLGFNNVYAQVTAQINFNSHEKTQEKYTPNTNYKNQAIRSRQS
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHCCCCCCCHHHHHHHHHH
TVNDKINNRKEENKPDELFPQTSFSSNKDLNSTTYSNKKIKKSIIQNNQDNNILHSNSAI
HHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEECCCCC
SHDDTINYELNHSLSHTKMNIGEIKRLSAAVIVNFVKDKNGKSVPINVEQIKKIKNLVRE
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
AIGYSKVRGDSVYVVNESFFQKNKNSPIKLLKDSNQSNFYSTFLTFTPWFISLFFLFFLV
HHCHHHCCCCEEEEEEHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
KKCFFSSSKNNINNQSYKNKTEEDLLEKDTKAENISELKFSKTSNTDKLIHQICNISNQN
HHHHHCCCCCCCCCCHHCCCCHHHHHHHCCCCCCHHHHEECCCCCHHHHHHHHHCCCCCC
PRIIASIIRQWMSDKK
CHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12089438