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The map label for this gene is holB

Identifier: 21672618

GI number: 21672618

Start: 387723

End: 388709

Strand: Direct

Name: holB

Synonym: BUsg342

Alternate gene names: 21672618

Gene position: 387723-388709 (Clockwise)

Preceding gene: 21672617

Following gene: 21672619

Centisome position: 60.44

GC content: 23.71

Gene sequence:

>987_bases
ATGAAATGGTATCCTTGGTTAATTAAACCATATAAAAAAATTATTAGACTGCATGAAAGAAAAAAAGCACATCATGCAAT
TTTAATAAAAACTCAAAAGGGAATGGGAGTTTTTAAACTAGTTTGGTTCATTAGTAAATGGTTACTATGCTTAAAACCCA
AAGGAACAAATTTTTGTGATAACTGCCATGGATGTAAACTAATGTCTGCAAATAATCATCCAGATTGGCATACTATAATT
AATGAAAAAAATGATGTCTTTGATGTTGATTCTATTCGAGTCATTAATGAAAAAATATTTAAACGTGCGCAACAAGGAAA
CAATAAAATCATTTTTTTACCAAATGTTCATAAATTAACAGAATCAGCAGTGAATGCATTATTAAAAATTTTAGAAGAAC
CACCAGAAAAAAATTGGTTTTTTCTAATAGATTACAATTATTTAAAATTACATTCTACATTAAAAAGTCGTTGTTTTTTA
TATAGATTACCCCTTCCCTTAGAAAAAGAAAGTTTAAATTGGTTAAAAAATAACAACAAAAAAGATAACATATCAAATTT
GACTTCATTACGTATTAATCAAGATTCTCCTATATCTGCAAAAAATTTTATTGAAAGCGATCTATGGGATGAAAGAAAAA
ATTTATATAAATCTTTATCACATTCTATTAAAGATAAAAACCTATTAAAAATATTGCCAGTCTTATGTAAAAAAAATACT
ATAATGAAAATAGATTGGATATGTTTACTATTATTTGATGCTGTAAAAACAAATTTCAACGAAAGAAAAAAATTAATCAA
CTGCGATCAAATAAAATTAATTAATTTTTTTTCTAAAAAATACAATAATATTCTTTTAAATAAAAGTATTCGAAAGTGGA
CAAAATGTCGATATATTTTATCAAGTGTATCTAGTATTAATAGTGAATTACTACTCTTAGAACAATTACTTTTATGGGAA
AAAATTTTATGTTTTATAACACCTTAA

Upstream 100 bases:

>100_bases
TAGATAAAAAAATAATAAAAATAAACGCTAATTTAAATATTAAAATTGTTACTCAAAACATCAAAAACCAAATTTTAAAA
TGGCTTCAACAAAAAGTCGT

Downstream 100 bases:

>100_bases
GTTCTACATAATTTTTAAATTAAAGAGAGAAAATTATGTTCTTAATTGATTCTCACTGCCATATTGATCAGATGAACATT
GATCAAAAAAATGATGATTT

Product: DNA polymerase III delta' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKWYPWLIKPYKKIIRLHERKKAHHAILIKTQKGMGVFKLVWFISKWLLCLKPKGTNFCDNCHGCKLMSANNHPDWHTII
NEKNDVFDVDSIRVINEKIFKRAQQGNNKIIFLPNVHKLTESAVNALLKILEEPPEKNWFFLIDYNYLKLHSTLKSRCFL
YRLPLPLEKESLNWLKNNNKKDNISNLTSLRINQDSPISAKNFIESDLWDERKNLYKSLSHSIKDKNLLKILPVLCKKNT
IMKIDWICLLLFDAVKTNFNERKKLINCDQIKLINFFSKKYNNILLNKSIRKWTKCRYILSSVSSINSELLLLEQLLLWE
KILCFITP

Sequences:

>Translated_328_residues
MKWYPWLIKPYKKIIRLHERKKAHHAILIKTQKGMGVFKLVWFISKWLLCLKPKGTNFCDNCHGCKLMSANNHPDWHTII
NEKNDVFDVDSIRVINEKIFKRAQQGNNKIIFLPNVHKLTESAVNALLKILEEPPEKNWFFLIDYNYLKLHSTLKSRCFL
YRLPLPLEKESLNWLKNNNKKDNISNLTSLRINQDSPISAKNFIESDLWDERKNLYKSLSHSIKDKNLLKILPVLCKKNT
IMKIDWICLLLFDAVKTNFNERKKLINCDQIKLINFFSKKYNNILLNKSIRKWTKCRYILSSVSSINSELLLLEQLLLWE
KILCFITP
>Mature_328_residues
MKWYPWLIKPYKKIIRLHERKKAHHAILIKTQKGMGVFKLVWFISKWLLCLKPKGTNFCDNCHGCKLMSANNHPDWHTII
NEKNDVFDVDSIRVINEKIFKRAQQGNNKIIFLPNVHKLTESAVNALLKILEEPPEKNWFFLIDYNYLKLHSTLKSRCFL
YRLPLPLEKESLNWLKNNNKKDNISNLTSLRINQDSPISAKNFIESDLWDERKNLYKSLSHSIKDKNLLKILPVLCKKNT
IMKIDWICLLLFDAVKTNFNERKKLINCDQIKLINFFSKKYNNILLNKSIRKWTKCRYILSSVSSINSELLLLEQLLLWE
KILCFITP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=324, Percent_Identity=33.641975308642, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1786676, Length=140, Percent_Identity=29.2857142857143, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOLB_BUCAP (Q8K9J2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660685.1
- ProteinModelPortal:   Q8K9J2
- SMR:   Q8K9J2
- EnsemblBacteria:   EBBUCT00000000475
- GeneID:   1005577
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg342
- GeneTree:   EBGT00050000008070
- HOGENOM:   HBG729459
- OMA:   CHSCHLM
- ProtClustDB:   CLSK493055
- BioCyc:   BAPH198804:BUSG342-MONOMER
- InterPro:   IPR008921
- InterPro:   IPR015199

Pfam domain/function: PF09115 DNApol3-delta_C; SSF48019 Pol_clamp_load_C

EC number: =2.7.7.7

Molecular weight: Translated: 38928; Mature: 38928

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWYPWLIKPYKKIIRLHERKKAHHAILIKTQKGMGVFKLVWFISKWLLCLKPKGTNFCD
CCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCC
NCHGCKLMSANNHPDWHTIINEKNDVFDVDSIRVINEKIFKRAQQGNNKIIFLPNVHKLT
CCCCCEEECCCCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
ESAVNALLKILEEPPEKNWFFLIDYNYLKLHSTLKSRCFLYRLPLPLEKESLNWLKNNNK
HHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHCCCC
KDNISNLTSLRINQDSPISAKNFIESDLWDERKNLYKSLSHSIKDKNLLKILPVLCKKNT
CCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IMKIDWICLLLFDAVKTNFNERKKLINCDQIKLINFFSKKYNNILLNKSIRKWTKCRYIL
EEEHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SSVSSINSELLLLEQLLLWEKILCFITP
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKWYPWLIKPYKKIIRLHERKKAHHAILIKTQKGMGVFKLVWFISKWLLCLKPKGTNFCD
CCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCC
NCHGCKLMSANNHPDWHTIINEKNDVFDVDSIRVINEKIFKRAQQGNNKIIFLPNVHKLT
CCCCCEEECCCCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
ESAVNALLKILEEPPEKNWFFLIDYNYLKLHSTLKSRCFLYRLPLPLEKESLNWLKNNNK
HHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHCCCC
KDNISNLTSLRINQDSPISAKNFIESDLWDERKNLYKSLSHSIKDKNLLKILPVLCKKNT
CCCCCHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
IMKIDWICLLLFDAVKTNFNERKKLINCDQIKLINFFSKKYNNILLNKSIRKWTKCRYIL
EEEHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SSVSSINSELLLLEQLLLWEKILCFITP
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438