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The map label for this gene is tmk [H]

Identifier: 21672617

GI number: 21672617

Start: 387085

End: 387726

Strand: Direct

Name: tmk [H]

Synonym: BUsg341

Alternate gene names: 21672617

Gene position: 387085-387726 (Clockwise)

Preceding gene: 21672616

Following gene: 21672618

Centisome position: 60.34

GC content: 25.86

Gene sequence:

>642_bases
ATGATGATAAAAAGTAAATTTATTGTAATTGAAGGATTAGAAGGTGCTGGAAAAACACACGCTTGTATTTGCGTCCAACG
TATTTTAAAAGAAAATAATATAAAGAATGTCATATTAGTACGTCAACCTGGAAGTACACCTGTTGCAGAAAAAATAAGAA
AATTAATAAAAAATAACACTTATATTGAAGATTTTGAAAAAGAGACAGAATTATTACTAATGTATGCTGCAAGAATACAA
CTAGTTAAAAAAATAATACAACCAGCGTTAAAAAAGGGAACTTGGGTCATTTCAGATCGTCATGATTTATCTTCTTTAGC
CTATCAAGGTGGCGGGTTGGGTATAAAAAGAAAACTAATCAATAAACTGAAATATTTATTTTTGCAAGATTTTATTCCTG
ATCTAACTATTTATTTAGATGTTTATCCTGAAATTGGTTTAAAAAGAGCATCAAAAAGAAATCATTTAGATCGAATAGAA
CATCGATCTTTAACATTTTTTAAAAAAACAAGAGCGAGCTATTTAAAAAATATAAAATTAGATAAAAAAATAATAAAAAT
AAACGCTAATTTAAATATTAAAATTGTTACTCAAAACATCAAAAACCAAATTTTAAAATGGCTTCAACAAAAAGTCGTAT
GA

Upstream 100 bases:

>100_bases
TACAAAAATCTATTGATTATATTAATAATAAAAATAAAAAATAAATTTTTATATTTATTCCTCAATAGTACAATTAAAAC
TGTACATAAAAAAATTAAAA

Downstream 100 bases:

>100_bases
AATGGTATCCTTGGTTAATTAAACCATATAAAAAAATTATTAGACTGCATGAAAGAAAAAAAGCACATCATGCAATTTTA
ATAAAAACTCAAAAGGGAAT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MMIKSKFIVIEGLEGAGKTHACICVQRILKENNIKNVILVRQPGSTPVAEKIRKLIKNNTYIEDFEKETELLLMYAARIQ
LVKKIIQPALKKGTWVISDRHDLSSLAYQGGGLGIKRKLINKLKYLFLQDFIPDLTIYLDVYPEIGLKRASKRNHLDRIE
HRSLTFFKKTRASYLKNIKLDKKIIKINANLNIKIVTQNIKNQILKWLQQKVV

Sequences:

>Translated_213_residues
MMIKSKFIVIEGLEGAGKTHACICVQRILKENNIKNVILVRQPGSTPVAEKIRKLIKNNTYIEDFEKETELLLMYAARIQ
LVKKIIQPALKKGTWVISDRHDLSSLAYQGGGLGIKRKLINKLKYLFLQDFIPDLTIYLDVYPEIGLKRASKRNHLDRIE
HRSLTFFKKTRASYLKNIKLDKKIIKINANLNIKIVTQNIKNQILKWLQQKVV
>Mature_213_residues
MMIKSKFIVIEGLEGAGKTHACICVQRILKENNIKNVILVRQPGSTPVAEKIRKLIKNNTYIEDFEKETELLLMYAARIQ
LVKKIIQPALKKGTWVISDRHDLSSLAYQGGGLGIKRKLINKLKYLFLQDFIPDLTIYLDVYPEIGLKRASKRNHLDRIE
HRSLTFFKKTRASYLKNIKLDKKIIKINANLNIKIVTQNIKNQILKWLQQKVV

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=210, Percent_Identity=48.0952380952381, Blast_Score=209, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 24679; Mature: 24679

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIKSKFIVIEGLEGAGKTHACICVQRILKENNIKNVILVRQPGSTPVAEKIRKLIKNNT
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCC
YIEDFEKETELLLMYAARIQLVKKIIQPALKKGTWVISDRHDLSSLAYQGGGLGIKRKLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCHHHHHHH
NKLKYLFLQDFIPDLTIYLDVYPEIGLKRASKRNHLDRIEHRSLTFFKKTRASYLKNIKL
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DKKIIKINANLNIKIVTQNIKNQILKWLQQKVV
CCEEEEEECCEEEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMIKSKFIVIEGLEGAGKTHACICVQRILKENNIKNVILVRQPGSTPVAEKIRKLIKNNT
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCC
YIEDFEKETELLLMYAARIQLVKKIIQPALKKGTWVISDRHDLSSLAYQGGGLGIKRKLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCHHHHHHH
NKLKYLFLQDFIPDLTIYLDVYPEIGLKRASKRNHLDRIEHRSLTFFKKTRASYLKNIKL
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DKKIIKINANLNIKIVTQNIKNQILKWLQQKVV
CCEEEEEECCEEEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]