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The map label for this gene is pfkA [H]

Identifier: 21672572

GI number: 21672572

Start: 338920

End: 339882

Strand: Direct

Name: pfkA [H]

Synonym: BUsg295

Alternate gene names: 21672572

Gene position: 338920-339882 (Clockwise)

Preceding gene: 21672571

Following gene: 21672573

Centisome position: 52.84

GC content: 33.33

Gene sequence:

>963_bases
ATGATAAAAAAGATCGGAGTATTAACTAGTGGTGGAGATGCTCCTGGGATGAATGCTGCGATTAGAGGTGTAGTAAGAAC
AGCATTGAGTGAAAAATTAGAAGTATTTGGTATTTATGATGGATATTTAGGTCTATATGAAAATCGTATGACATTGTTAG
ATAGATATAGTGTTTCTGATATGATTAATAGAGGCGGAACATTTTTAGGATCTGCTCGATTTCCTGGCTTTTACAAAAAT
GAAATACGTACTATTGCAGTAAATAATTTAAATAAAAGAAATATAGATGCTCTTGTTGTTATCGGCGGTGATGGATCTTA
TATAGGAGCACAAAAATTAACGAAAATGGGAATTCCATGTATTAGTATTCCAGGTACAATAGACAACGATGTAGCAGGTA
CTGATTACACGATAGGTTATTTTACAGCCTTAGAGACAGTGGTTGAAGCGATTGATAGATTAAGAGATACTTCTTCTTCT
CATCAAAGAATTTCTATTGTAGAAGTTATGGGGCGATACTGCGGAGATTTAACATTAGCTGCTGCAATAGCAGGTGGTTG
CGAATTTATTGTTCTTCCAGAAATTCATTATAAAAAAGAAGAATTGGTTTCTGAAATTCAGGCAGGAATCGAAAAAGGGA
AAAAACATGCAATAGTCGCAATAACAGAATATATTTGTGATGTAGAAAAATTAGCGAAATATATTGAGAAGAAAACCAAT
AGAGAGACAAGAGCTACAATTTTAGGACATATCCAGAGAGGAGGAGCACCAGTAGTATATGATAGAATTCTTGCTTCAAG
AATGGGAGCATATGCAGTAGAATTGCTGATAGAAGGCTACAAAGGGAAATGTGTTGGAATACAAAATGAAAAAATGGTAT
TTAACGACATAACAGATGCGTTAAAAAATATGAAACGTGTTTTTAAAAAAGATTGGTTAATTACAGCTAAAAAGTTATAC
TAA

Upstream 100 bases:

>100_bases
TATATATTGTAAAAATACATTTTTTATTAAGTTGAAATTTTATCAATAATCAATAATAGGAAAAACAAATTTTTTTATAG
TAGTTAAAAACGGGATTTTT

Downstream 100 bases:

>100_bases
TATAAAATTAGTGCCGGTTTTTCCGGCGCTCTAAAATTTTAATTTTATTTTTAAATAAATAAAATTATTTAGGTATTCAA
ATAATGAATTTTTGTAGTAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIGVLTSGGDAPGMNAAIRGVVRTALSEKLEVFGIYDGYLGLYENRMTLLDRYSVSDMINRGGTFLGSARFPGFYKN
EIRTIAVNNLNKRNIDALVVIGGDGSYIGAQKLTKMGIPCISIPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEFIVLPEIHYKKEELVSEIQAGIEKGKKHAIVAITEYICDVEKLAKYIEKKTN
RETRATILGHIQRGGAPVVYDRILASRMGAYAVELLIEGYKGKCVGIQNEKMVFNDITDALKNMKRVFKKDWLITAKKLY

Sequences:

>Translated_320_residues
MIKKIGVLTSGGDAPGMNAAIRGVVRTALSEKLEVFGIYDGYLGLYENRMTLLDRYSVSDMINRGGTFLGSARFPGFYKN
EIRTIAVNNLNKRNIDALVVIGGDGSYIGAQKLTKMGIPCISIPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEFIVLPEIHYKKEELVSEIQAGIEKGKKHAIVAITEYICDVEKLAKYIEKKTN
RETRATILGHIQRGGAPVVYDRILASRMGAYAVELLIEGYKGKCVGIQNEKMVFNDITDALKNMKRVFKKDWLITAKKLY
>Mature_320_residues
MIKKIGVLTSGGDAPGMNAAIRGVVRTALSEKLEVFGIYDGYLGLYENRMTLLDRYSVSDMINRGGTFLGSARFPGFYKN
EIRTIAVNNLNKRNIDALVVIGGDGSYIGAQKLTKMGIPCISIPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEFIVLPEIHYKKEELVSEIQAGIEKGKKHAIVAITEYICDVEKLAKYIEKKTN
RETRATILGHIQRGGAPVVYDRILASRMGAYAVELLIEGYKGKCVGIQNEKMVFNDITDALKNMKRVFKKDWLITAKKLY

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=39.871382636656, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=39.1025641025641, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=39.1025641025641, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=39.1025641025641, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=39.1025641025641, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=39.2282958199357, Blast_Score=188, Evalue=6e-48,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=78.4375, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=38.5852090032154, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=36.2179487179487, Blast_Score=180, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI72003601, Length=346, Percent_Identity=29.1907514450867, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=347, Percent_Identity=37.463976945245, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323861, Length=347, Percent_Identity=33.7175792507205, Blast_Score=173, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28573326, Length=317, Percent_Identity=39.7476340694006, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI17647809, Length=316, Percent_Identity=39.873417721519, Blast_Score=200, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24652337, Length=316, Percent_Identity=39.873417721519, Blast_Score=200, Evalue=9e-52,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35239; Mature: 35239

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIGVLTSGGDAPGMNAAIRGVVRTALSEKLEVFGIYDGYLGLYENRMTLLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPGFYKNEIRTIAVNNLNKRNIDALVVIGGDGSYIGAQKLTKMGIPC
HHHCCCCEEECCCCCCHHHCCEEEEEECCCCCCCCCEEEEECCCCCEECHHHHHHHCCCE
ISIPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTLA
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHH
AAIAGGCEFIVLPEIHYKKEELVSEIQAGIEKGKKHAIVAITEYICDVEKLAKYIEKKTN
HHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHHHHHCC
RETRATILGHIQRGGAPVVYDRILASRMGAYAVELLIEGYKGKCVGIQNEKMVFNDITDA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHH
LKNMKRVFKKDWLITAKKLY
HHHHHHHHHHHHHEEEHHCC
>Mature Secondary Structure
MIKKIGVLTSGGDAPGMNAAIRGVVRTALSEKLEVFGIYDGYLGLYENRMTLLDRYSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHH
MINRGGTFLGSARFPGFYKNEIRTIAVNNLNKRNIDALVVIGGDGSYIGAQKLTKMGIPC
HHHCCCCEEECCCCCCHHHCCEEEEEECCCCCCCCCEEEEECCCCCEECHHHHHHHCCCE
ISIPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTLA
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHH
AAIAGGCEFIVLPEIHYKKEELVSEIQAGIEKGKKHAIVAITEYICDVEKLAKYIEKKTN
HHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHCHHHHHHHHHHHCC
RETRATILGHIQRGGAPVVYDRILASRMGAYAVELLIEGYKGKCVGIQNEKMVFNDITDA
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHH
LKNMKRVFKKDWLITAKKLY
HHHHHHHHHHHHHEEEHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA