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The map label for this gene is glpF

Identifier: 21672573

GI number: 21672573

Start: 339966

End: 340754

Strand: Direct

Name: glpF

Synonym: BUsg296

Alternate gene names: 21672573

Gene position: 339966-340754 (Clockwise)

Preceding gene: 21672572

Following gene: 21672580

Centisome position: 53.0

GC content: 21.8

Gene sequence:

>789_bases
ATGAATTTTTGTAGTAAAAAAAAAATATTAAAACAATGTTTTTTTGAATTTTTAGGAACAGGTTTAATAATATTTTTAGG
AATTAGTTCTCTTGTCGTATCAAAATTAACAAATTTTCATTTTAATCACTGTGAAATTAGTTGTATTTGGGGATTAGGAG
TATTTATATCAATTTGTTTTTGTTCTTCAGTCTCTGGAGCGCATTTAAATCCAGCTATTACTATTTTTCTTTTTTTATCT
TCTCAATTTAATAAAAAAAAAGTAATACCGTATATTCTATCTCAAATATCTGGTACTTTTTTCTTTACTTTTTTAATTTA
TCTTATTTTTAATAATCTATTAAATTCATTTGAGTCTAAATACAATATTGTTCGAGGAACAAAAAAAAGTCTTGAATTAG
CTTCATTATTCTGCGTTTTTCCTAAAGAAAATTATAATTTTATTCATGATTTTATATTAGAAATATTAATTGGAATTATT
TTTATAATTATATTGATGAAATTGAGTGAAAAAAATAATTTATTTAAATTTTATAAATTTATCAATCCCTTTTTAATAGG
AACATTAGTGATAATAATTAATTTATTTTTAACTTCATATAGTAATATTACTTTAAATCCAGCTCGAGATTTAGGTCCTA
GAATATTTTTAAGTTTAATAGGGTGGGGAAAATTAGCATTTACAGGCGATGATAATATCATTTTTCCTTATTTTTTAATA
CCCACTATTGCTCCTATTATCGGTATCAATTTAGGTGGATGGATATATATCCTTTATATAAAAAAATAG

Upstream 100 bases:

>100_bases
CTAAAAAGTTATACTAATATAAAATTAGTGCCGGTTTTTCCGGCGCTCTAAAATTTTAATTTTATTTTTAAATAAATAAA
ATTATTTAGGTATTCAAATA

Downstream 100 bases:

>100_bases
TTTTTTATAAAAAACTATCTGATATTTTTATAATTTTTAAAAATTCTTCATGTTTTAAAGATGCACTACCGATTAATAAT
CCATCAATATCAGGTTGTTT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MNFCSKKKILKQCFFEFLGTGLIIFLGISSLVVSKLTNFHFNHCEISCIWGLGVFISICFCSSVSGAHLNPAITIFLFLS
SQFNKKKVIPYILSQISGTFFFTFLIYLIFNNLLNSFESKYNIVRGTKKSLELASLFCVFPKENYNFIHDFILEILIGII
FIIILMKLSEKNNLFKFYKFINPFLIGTLVIIINLFLTSYSNITLNPARDLGPRIFLSLIGWGKLAFTGDDNIIFPYFLI
PTIAPIIGINLGGWIYILYIKK

Sequences:

>Translated_262_residues
MNFCSKKKILKQCFFEFLGTGLIIFLGISSLVVSKLTNFHFNHCEISCIWGLGVFISICFCSSVSGAHLNPAITIFLFLS
SQFNKKKVIPYILSQISGTFFFTFLIYLIFNNLLNSFESKYNIVRGTKKSLELASLFCVFPKENYNFIHDFILEILIGII
FIIILMKLSEKNNLFKFYKFINPFLIGTLVIIINLFLTSYSNITLNPARDLGPRIFLSLIGWGKLAFTGDDNIIFPYFLI
PTIAPIIGINLGGWIYILYIKK
>Mature_262_residues
MNFCSKKKILKQCFFEFLGTGLIIFLGISSLVVSKLTNFHFNHCEISCIWGLGVFISICFCSSVSGAHLNPAITIFLFLS
SQFNKKKVIPYILSQISGTFFFTFLIYLIFNNLLNSFESKYNIVRGTKKSLELASLFCVFPKENYNFIHDFILEILIGII
FIIILMKLSEKNNLFKFYKFINPFLIGTLVIIINLFLTSYSNITLNPARDLGPRIFLSLIGWGKLAFTGDDNIIFPYFLI
PTIAPIIGINLGGWIYILYIKK

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family

Homologues:

Organism=Homo sapiens, GI157266307, Length=257, Percent_Identity=29.1828793774319, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI22538420, Length=260, Percent_Identity=28.0769230769231, Blast_Score=111, Evalue=8e-25,
Organism=Homo sapiens, GI4502187, Length=256, Percent_Identity=26.953125, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI4826645, Length=258, Percent_Identity=26.3565891472868, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI310133356, Length=205, Percent_Identity=26.3414634146341, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI310114181, Length=205, Percent_Identity=26.3414634146341, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=41.6988416988417, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787101, Length=223, Percent_Identity=23.7668161434978, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17531431, Length=258, Percent_Identity=26.3565891472868, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531429, Length=258, Percent_Identity=26.3565891472868, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17544068, Length=261, Percent_Identity=26.8199233716475, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71992966, Length=258, Percent_Identity=25.5813953488372, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564052, Length=249, Percent_Identity=27.3092369477912, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17533613, Length=249, Percent_Identity=27.3092369477912, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71994009, Length=256, Percent_Identity=27.34375, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321054, Length=230, Percent_Identity=33.4782608695652, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322985, Length=219, Percent_Identity=27.8538812785388, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLPF_BUCAP (Q8K9M9)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660640.1
- ProteinModelPortal:   Q8K9M9
- SMR:   Q8K9M9
- EnsemblBacteria:   EBBUCT00000000501
- GeneID:   1005500
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg296
- GeneTree:   EBGT00050000008151
- HOGENOM:   HBG705794
- OMA:   CAGLLIY
- ProtClustDB:   CLSK315874
- BioCyc:   BAPH198804:BUSG296-MONOMER
- InterPro:   IPR000425
- InterPro:   IPR022357
- PANTHER:   PTHR19139
- PRINTS:   PR00783

Pfam domain/function: PF00230 MIP

EC number: NA

Molecular weight: Translated: 29939; Mature: 29939

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc221040)-; HASH(0xc701914)-; HASH(0xb376ab8)-; HASH(0xbf3f574)-; HASH(0xb7002d8)-; HASH(0xc5c8174)-;

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFCSKKKILKQCFFEFLGTGLIIFLGISSLVVSKLTNFHFNHCEISCIWGLGVFISICF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHH
CSSVSGAHLNPAITIFLFLSSQFNKKKVIPYILSQISGTFFFTFLIYLIFNNLLNSFESK
HCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNIVRGTKKSLELASLFCVFPKENYNFIHDFILEILIGIIFIIILMKLSEKNNLFKFYKF
HHHHHCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
INPFLIGTLVIIINLFLTSYSNITLNPARDLGPRIFLSLIGWGKLAFTGDDNIIFPYFLI
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCHHHHHHHHCCCEEEEECCCCEEEHHHHH
PTIAPIIGINLGGWIYILYIKK
HHHHHHHHCCCCCEEEEEEEEH
>Mature Secondary Structure
MNFCSKKKILKQCFFEFLGTGLIIFLGISSLVVSKLTNFHFNHCEISCIWGLGVFISICF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHH
CSSVSGAHLNPAITIFLFLSSQFNKKKVIPYILSQISGTFFFTFLIYLIFNNLLNSFESK
HCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNIVRGTKKSLELASLFCVFPKENYNFIHDFILEILIGIIFIIILMKLSEKNNLFKFYKF
HHHHHCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
INPFLIGTLVIIINLFLTSYSNITLNPARDLGPRIFLSLIGWGKLAFTGDDNIIFPYFLI
HHHHHHHHHHHHHHHHHHCCCCEEECCHHHCCHHHHHHHHCCCEEEEECCCCEEEHHHHH
PTIAPIIGINLGGWIYILYIKK
HHHHHHHHCCCCCEEEEEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12089438