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The map label for this gene is hisS

Identifier: 21672557

GI number: 21672557

Start: 315534

End: 316820

Strand: Direct

Name: hisS

Synonym: BUsg277

Alternate gene names: 21672557

Gene position: 315534-316820 (Clockwise)

Preceding gene: 21672556

Following gene: 21672558

Centisome position: 49.19

GC content: 23.7

Gene sequence:

>1287_bases
GTGAATAAAAAAATTAATTCAGTAAGAGGAATGCACGATTATCTTCCTGAAGAATTAAAAATTTGGAAAAAAATAGAAAA
TATTATTCAAAAAATATTAACTAGTTATTGTTATGAAGAAATTAGATTACCTATATTAGAAAAAACTAAAATTTTTCAAA
GAGCTATTGGAAATATAACTGATGTAGTTGAAAAAGAAATGTATTCTTTTAAAGATAAAAAGGGAAATAGTTTAACTTTG
CGTCCTGAAGGAACGGTGGGTTGTGTGCGTGCTATAATAGAAAATAATCTATTATTTCAAAAAAAACAAAGATTTTGGTA
TTTAGGTCCTATGTTTAGATATGAAAGGCCACAAAAGGGAAGATATCGTCAATTTTATCAGTTAGGTGTTGAAGTATTTG
GATTGAATACACCAGATATTGATTTAGAAATTATTTTATTAATAAGTCGTTTATGGAAATGTTTAGGTATTAATCTTCAT
ATAAAATTAGAAATTAATTCAATTGGTTCAAAATTAGATCGTATTAAATATCAAAAAGAGTTAGTTTTTTTTCTTGAAAA
ATATAAAAATATTTTAGATGAAGATTCTAAAAAACGATTATATACTAATCCTTTTAGGATCTTAGATTCTAAAAACGACA
TGATGCAGAAAATTTTAAAAAAAGCTCCATTATTAAAAAATTATATCAATACTTCTGCAATTAATCACTTTAATAGTCTA
TGTAATATGATGGATTCATATGGAATCAAATATATACACAACCAAAATTTAGTACGTGGGTTAGATTATTATAATAGTAC
TGTATTTGAATGGAAAATTGATCAAATTGGATCACAAAATACTATTTGTGCTGGCGGGAGATATGATTCTTTAGTTCAAG
AATTAGGTGGAAATAAAACATCTGCAATAGGATTTGCAATAGGTATTGAACGTTTAGTTTTATTAATAAAATCATTAAAT
ATTATCTCTTGCAAAGAAGAAGACATCAATATCTATATCATATTTATAGGAGAATTAAATAAAATTTATGCAATAAGCTT
ATCAGAAGAAATAAGAAATATATATCCTAAATTAAAAATATTTGTAGATTTCATGACTTGTAGTCTTTCAAAAAAAATAA
AAAATGCTGTAGATTCTCTAGCTCATATTGCAATTTTAATAGGTGCAAACGAAATAAAAAAAAATTGTTTTTTATTAAAA
GATTTAAAAAGGGGGAGGGAATATTTTTTTTCAAAAAGTGAATTAATATTAAAGATTAAAAAAATTTTTTACAAACAAGG
TAATTAA

Upstream 100 bases:

>100_bases
AGATTAAAATTCGTAAAAAAGCAAAATCATTAGATAAATTAAAAAAAATTATTTAACTACGATTAAAAAATAAACATAAG
AAAAATAATAAGAGAAAATA

Downstream 100 bases:

>100_bases
AAGAAAATATTTTTATAAAATTTCGTTATATATGTTTGATATTTATCTTATTTTAAATTTATAGGAAAAAAATGTTTAAT
ACAAAAATAGAATTTTCAAA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNKKINSVRGMHDYLPEELKIWKKIENIIQKILTSYCYEEIRLPILEKTKIFQRAIGNITDVVEKEMYSFKDKKGNSLTL
RPEGTVGCVRAIIENNLLFQKKQRFWYLGPMFRYERPQKGRYRQFYQLGVEVFGLNTPDIDLEIILLISRLWKCLGINLH
IKLEINSIGSKLDRIKYQKELVFFLEKYKNILDEDSKKRLYTNPFRILDSKNDMMQKILKKAPLLKNYINTSAINHFNSL
CNMMDSYGIKYIHNQNLVRGLDYYNSTVFEWKIDQIGSQNTICAGGRYDSLVQELGGNKTSAIGFAIGIERLVLLIKSLN
IISCKEEDINIYIIFIGELNKIYAISLSEEIRNIYPKLKIFVDFMTCSLSKKIKNAVDSLAHIAILIGANEIKKNCFLLK
DLKRGREYFFSKSELILKIKKIFYKQGN

Sequences:

>Translated_428_residues
MNKKINSVRGMHDYLPEELKIWKKIENIIQKILTSYCYEEIRLPILEKTKIFQRAIGNITDVVEKEMYSFKDKKGNSLTL
RPEGTVGCVRAIIENNLLFQKKQRFWYLGPMFRYERPQKGRYRQFYQLGVEVFGLNTPDIDLEIILLISRLWKCLGINLH
IKLEINSIGSKLDRIKYQKELVFFLEKYKNILDEDSKKRLYTNPFRILDSKNDMMQKILKKAPLLKNYINTSAINHFNSL
CNMMDSYGIKYIHNQNLVRGLDYYNSTVFEWKIDQIGSQNTICAGGRYDSLVQELGGNKTSAIGFAIGIERLVLLIKSLN
IISCKEEDINIYIIFIGELNKIYAISLSEEIRNIYPKLKIFVDFMTCSLSKKIKNAVDSLAHIAILIGANEIKKNCFLLK
DLKRGREYFFSKSELILKIKKIFYKQGN
>Mature_428_residues
MNKKINSVRGMHDYLPEELKIWKKIENIIQKILTSYCYEEIRLPILEKTKIFQRAIGNITDVVEKEMYSFKDKKGNSLTL
RPEGTVGCVRAIIENNLLFQKKQRFWYLGPMFRYERPQKGRYRQFYQLGVEVFGLNTPDIDLEIILLISRLWKCLGINLH
IKLEINSIGSKLDRIKYQKELVFFLEKYKNILDEDSKKRLYTNPFRILDSKNDMMQKILKKAPLLKNYINTSAINHFNSL
CNMMDSYGIKYIHNQNLVRGLDYYNSTVFEWKIDQIGSQNTICAGGRYDSLVQELGGNKTSAIGFAIGIERLVLLIKSLN
IISCKEEDINIYIIFIGELNKIYAISLSEEIRNIYPKLKIFVDFMTCSLSKKIKNAVDSLAHIAILIGANEIKKNCFLLK
DLKRGREYFFSKSELILKIKKIFYKQGN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=50.1182033096927, Blast_Score=479, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24643061, Length=385, Percent_Identity=22.0779220779221, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_BUCAP (Q8K9P3)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660624.1
- ProteinModelPortal:   Q8K9P3
- SMR:   Q8K9P3
- EnsemblBacteria:   EBBUCT00000000344
- GeneID:   1005481
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg277
- GeneTree:   EBGT00050000007769
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   BAPH198804:BUSG277-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 50054; Mature: 50054

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKINSVRGMHDYLPEELKIWKKIENIIQKILTSYCYEEIRLPILEKTKIFQRAIGNIT
CCCCHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DVVEKEMYSFKDKKGNSLTLRPEGTVGCVRAIIENNLLFQKKQRFWYLGPMFRYERPQKG
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCHHEEHHCCEEEECCHHHCCCCCCC
RYRQFYQLGVEVFGLNTPDIDLEIILLISRLWKCLGINLHIKLEINSIGSKLDRIKYQKE
HHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHH
LVFFLEKYKNILDEDSKKRLYTNPFRILDSKNDMMQKILKKAPLLKNYINTSAINHFNSL
HHHHHHHHHHHHCCCCCCCEECCCHHEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
CNMMDSYGIKYIHNQNLVRGLDYYNSTVFEWKIDQIGSQNTICAGGRYDSLVQELGGNKT
HHHHHHCCEEEEECCHHHHHHHHHCCEEEEEEHHHCCCCCCEECCCCHHHHHHHHCCCCC
SAIGFAIGIERLVLLIKSLNIISCKEEDINIYIIFIGELNKIYAISLSEEIRNIYPKLKI
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHH
FVDFMTCSLSKKIKNAVDSLAHIAILIGANEIKKNCFLLKDLKRGREYFFSKSELILKIK
HHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KIFYKQGN
HHHHCCCC
>Mature Secondary Structure
MNKKINSVRGMHDYLPEELKIWKKIENIIQKILTSYCYEEIRLPILEKTKIFQRAIGNIT
CCCCHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
DVVEKEMYSFKDKKGNSLTLRPEGTVGCVRAIIENNLLFQKKQRFWYLGPMFRYERPQKG
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCHHEEHHCCEEEECCHHHCCCCCCC
RYRQFYQLGVEVFGLNTPDIDLEIILLISRLWKCLGINLHIKLEINSIGSKLDRIKYQKE
HHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHH
LVFFLEKYKNILDEDSKKRLYTNPFRILDSKNDMMQKILKKAPLLKNYINTSAINHFNSL
HHHHHHHHHHHHCCCCCCCEECCCHHEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
CNMMDSYGIKYIHNQNLVRGLDYYNSTVFEWKIDQIGSQNTICAGGRYDSLVQELGGNKT
HHHHHHCCEEEEECCHHHHHHHHHCCEEEEEEHHHCCCCCCEECCCCHHHHHHHHCCCCC
SAIGFAIGIERLVLLIKSLNIISCKEEDINIYIIFIGELNKIYAISLSEEIRNIYPKLKI
HHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCEEEEEEHHHHHHHHHHHHHH
FVDFMTCSLSKKIKNAVDSLAHIAILIGANEIKKNCFLLKDLKRGREYFFSKSELILKIK
HHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
KIFYKQGN
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438