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The map label for this gene is glyA

Identifier: 21672558

GI number: 21672558

Start: 316892

End: 318145

Strand: Direct

Name: glyA

Synonym: BUsg278

Alternate gene names: 21672558

Gene position: 316892-318145 (Clockwise)

Preceding gene: 21672557

Following gene: 21672560

Centisome position: 49.4

GC content: 29.51

Gene sequence:

>1254_bases
ATGTTTAATACAAAAATAGAATTTTCAAAATATGATCCAGAATTATGGAAAGCGATAGATCAAGAAAAAAATAGACAAGA
AAACCATATAGAATTAATCGCATCAGAAAATTATACTAGTAATTACGTTATGCATGTACAAGGTTCACAATTAACTAACA
AATATGCAGAAGGTTATCCTGAAAAACGTTACTATGGTGGTTGCAAATATGTAGATATTATAGAAAAATTAGCGATTAAT
CGAGCTAAAAAACTCTTTAATGCTGATTATGCCAACGTTCAACCTCATTCAGGATCTCAAGCTAATTTTGCTGTTTATAC
AGCACTGTTAAATCCTGGTGATACAATATTAGGCATGAAATTATCGCACGGAGGTCATTTAACACATGGTTCTTCTGTAA
ATTTTTCAGGGAAAACATATAATGTTATTGGGTATGGAGTAGATAAAAAAGGAAATATTGACTATCAAGAAATTTTAACA
TTAGCAAAGAAATATAAACCTAAAATGATTATTGGTGGTTTTTCAGCCTATTCTGGCATTTGTAATTGGTCAAAAATGCG
CGATATTGCTGACGAAATTAACGCTTATTTTGTTGTTGATATTGCTCACGTTGCTGGTTTAATAGCGGCGAATCTTTATC
CTAATCCTATAGATTATGCACATGTTGTAACAAGTACGACACATAAAACATTAGCAGGTCCTCGAGGTGGTTTAATTTTA
GCTAAAAATGGAACTAATACTTTCTATAAAAAAATAAATTTATCTGTTTTTCCAGGAGCACAAGGTGGACCGCTTATGCA
TGTTATTGCAGCAAAAGCTATTGCTTTTAAAGAAGCTTTAGAACCTGCTTTTAAAATATATCAAAAACAGGTAATTAAAA
ATGCTCAAGTTATGGTTCAATCTTTTTTAAAAAAAGATTATCAAATTGTATCCGGAAACACATTTAATCATTTATTTTTA
CTAGATTTAACAAGCAAAAATATAACTGGACAAGAAGCTGATATTGCTTTGGGTAAATGTAATATAACTGTTAATAAAAA
TACTATTCCAAATGATCTTAGAAGTCCTTTTATTACATCAGGAATTCGTATTGGGACACCTGCGGTTACAAAAAGAGGTT
TTAAAGAAAACGAAATGTTACAAATATCAGATTGGATCGTTCATATTTTAAATAATATTAAAGATAAAAATAGTCTTCTT
GGCATAAAAAATGAAGTATTAAAACTCTGTTCAAAATATCCTGTCTATATTTAA

Upstream 100 bases:

>100_bases
AAAAAATTTTTTACAAACAAGGTAATTAAAAGAAAATATTTTTATAAAATTTCGTTATATATGTTTGATATTTATCTTAT
TTTAAATTTATAGGAAAAAA

Downstream 100 bases:

>100_bases
ATCAAAAAAAGCTTAGTATATCCATCTTTGTTAAAGATAATATTAATTATAATAATAATAAATTTTTGAAAAAAATTTAC
TTTTATATTTTATTCAGGAA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFNTKIEFSKYDPELWKAIDQEKNRQENHIELIASENYTSNYVMHVQGSQLTNKYAEGYPEKRYYGGCKYVDIIEKLAIN
RAKKLFNADYANVQPHSGSQANFAVYTALLNPGDTILGMKLSHGGHLTHGSSVNFSGKTYNVIGYGVDKKGNIDYQEILT
LAKKYKPKMIIGGFSAYSGICNWSKMRDIADEINAYFVVDIAHVAGLIAANLYPNPIDYAHVVTSTTHKTLAGPRGGLIL
AKNGTNTFYKKINLSVFPGAQGGPLMHVIAAKAIAFKEALEPAFKIYQKQVIKNAQVMVQSFLKKDYQIVSGNTFNHLFL
LDLTSKNITGQEADIALGKCNITVNKNTIPNDLRSPFITSGIRIGTPAVTKRGFKENEMLQISDWIVHILNNIKDKNSLL
GIKNEVLKLCSKYPVYI

Sequences:

>Translated_417_residues
MFNTKIEFSKYDPELWKAIDQEKNRQENHIELIASENYTSNYVMHVQGSQLTNKYAEGYPEKRYYGGCKYVDIIEKLAIN
RAKKLFNADYANVQPHSGSQANFAVYTALLNPGDTILGMKLSHGGHLTHGSSVNFSGKTYNVIGYGVDKKGNIDYQEILT
LAKKYKPKMIIGGFSAYSGICNWSKMRDIADEINAYFVVDIAHVAGLIAANLYPNPIDYAHVVTSTTHKTLAGPRGGLIL
AKNGTNTFYKKINLSVFPGAQGGPLMHVIAAKAIAFKEALEPAFKIYQKQVIKNAQVMVQSFLKKDYQIVSGNTFNHLFL
LDLTSKNITGQEADIALGKCNITVNKNTIPNDLRSPFITSGIRIGTPAVTKRGFKENEMLQISDWIVHILNNIKDKNSLL
GIKNEVLKLCSKYPVYI
>Mature_417_residues
MFNTKIEFSKYDPELWKAIDQEKNRQENHIELIASENYTSNYVMHVQGSQLTNKYAEGYPEKRYYGGCKYVDIIEKLAIN
RAKKLFNADYANVQPHSGSQANFAVYTALLNPGDTILGMKLSHGGHLTHGSSVNFSGKTYNVIGYGVDKKGNIDYQEILT
LAKKYKPKMIIGGFSAYSGICNWSKMRDIADEINAYFVVDIAHVAGLIAANLYPNPIDYAHVVTSTTHKTLAGPRGGLIL
AKNGTNTFYKKINLSVFPGAQGGPLMHVIAAKAIAFKEALEPAFKIYQKQVIKNAQVMVQSFLKKDYQIVSGNTFNHLFL
LDLTSKNITGQEADIALGKCNITVNKNTIPNDLRSPFITSGIRIGTPAVTKRGFKENEMLQISDWIVHILNNIKDKNSLL
GIKNEVLKLCSKYPVYI

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=418, Percent_Identity=44.0191387559809, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI261862350, Length=418, Percent_Identity=44.0191387559809, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI261862348, Length=418, Percent_Identity=44.0191387559809, Blast_Score=358, Evalue=6e-99,
Organism=Homo sapiens, GI19923315, Length=418, Percent_Identity=44.0191387559809, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI261862346, Length=413, Percent_Identity=44.0677966101695, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=43.142144638404, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=41.3881748071979, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=67.7884615384615, Blast_Score=603, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25144732, Length=398, Percent_Identity=43.7185929648241, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI25144729, Length=399, Percent_Identity=43.609022556391, Blast_Score=323, Evalue=9e-89,
Organism=Saccharomyces cerevisiae, GI6319739, Length=437, Percent_Identity=42.1052631578947, Blast_Score=348, Evalue=9e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=397, Percent_Identity=42.0654911838791, Blast_Score=309, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24640005, Length=404, Percent_Identity=44.3069306930693, Blast_Score=344, Evalue=6e-95,
Organism=Drosophila melanogaster, GI221329721, Length=404, Percent_Identity=44.3069306930693, Blast_Score=343, Evalue=9e-95,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_BUCAP (Q8K9P2)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660625.1
- ProteinModelPortal:   Q8K9P2
- SMR:   Q8K9P2
- EnsemblBacteria:   EBBUCT00000000145
- GeneID:   1005482
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg278
- GeneTree:   EBGT00050000008150
- HOGENOM:   HBG301263
- OMA:   PEEMNID
- ProtClustDB:   PRK00011
- BioCyc:   BAPH198804:BUSG278-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR008767
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412
- TIGRFAMs:   TIGR01563

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46433; Mature: 46433

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNTKIEFSKYDPELWKAIDQEKNRQENHIELIASENYTSNYVMHVQGSQLTNKYAEGYP
CCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHCCCC
EKRYYGGCKYVDIIEKLAINRAKKLFNADYANVQPHSGSQANFAVYTALLNPGDTILGMK
CCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEE
LSHGGHLTHGSSVNFSGKTYNVIGYGVDKKGNIDYQEILTLAKKYKPKMIIGGFSAYSGI
ECCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHCC
CNWSKMRDIADEINAYFVVDIAHVAGLIAANLYPNPIDYAHVVTSTTHKTLAGPRGGLIL
CCHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCCCCCHHHEEECCCHHHHCCCCCCEEE
AKNGTNTFYKKINLSVFPGAQGGPLMHVIAAKAIAFKEALEPAFKIYQKQVIKNAQVMVQ
EECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFLKKDYQIVSGNTFNHLFLLDLTSKNITGQEADIALGKCNITVNKNTIPNDLRSPFITS
HHHHCCCEEECCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCHHHCCCHHHC
GIRIGTPAVTKRGFKENEMLQISDWIVHILNNIKDKNSLLGIKNEVLKLCSKYPVYI
CEEECCCHHHHCCCCCCCEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFNTKIEFSKYDPELWKAIDQEKNRQENHIELIASENYTSNYVMHVQGSQLTNKYAEGYP
CCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEECCHHHHHHHCCCC
EKRYYGGCKYVDIIEKLAINRAKKLFNADYANVQPHSGSQANFAVYTALLNPGDTILGMK
CCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEE
LSHGGHLTHGSSVNFSGKTYNVIGYGVDKKGNIDYQEILTLAKKYKPKMIIGGFSAYSGI
ECCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHCC
CNWSKMRDIADEINAYFVVDIAHVAGLIAANLYPNPIDYAHVVTSTTHKTLAGPRGGLIL
CCHHHHHHHHHHCCEEEEEEHHHHHHHHHHCCCCCCCCHHHEEECCCHHHHCCCCCCEEE
AKNGTNTFYKKINLSVFPGAQGGPLMHVIAAKAIAFKEALEPAFKIYQKQVIKNAQVMVQ
EECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFLKKDYQIVSGNTFNHLFLLDLTSKNITGQEADIALGKCNITVNKNTIPNDLRSPFITS
HHHHCCCEEECCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCHHHCCCHHHC
GIRIGTPAVTKRGFKENEMLQISDWIVHILNNIKDKNSLLGIKNEVLKLCSKYPVYI
CEEECCCHHHHCCCCCCCEEEHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438